Mechanisms behind the varying severity of Aleutian mink disease virus: Comparison of three farms with a different disease status.

Aleutian mink disease virus (AMDV) is distributed widely among mink farms and wild mustelids despite ongoing attempts to stop the spread. The severity of Aleutian disease (AD) varies from subclinical to fatal but the reasons for its varying severity are complex and unclear. Recently, breeding of tolerant mink has drawn attention as the possible solution to reduce the effects of AD in farms. The aim of this study was to gather information on the effects of breeding based on overall health, production traits, and antibody titer on AD severity by comparing a positive farm (farm 1) that has been breeding for tolerance in mink to an infected farm without tolerance selection, and an AMDV-free farm. During the 2.5-year follow-up, the mink in farm 1 remained mostly free of clinical AD, had normal pelt quality and litter size, and had low virus copy numbers in tissues and low antibody titers in ELISA. In histopathological studies, most of the farm 1 mink had no/mild lesions in their kidneys. 29-43% of the mink were ELISA negative but PCR positive throughout the follow-up and frequent changes in virus strains and coinfections were observed. Several differences in gene expression between animals from different farms were also detected. These results indicate that the disease burden of AMDV can be reduced, with seemingly normal health and production rates, despite continual circulation of ADMV in cases where eradication attempts are unsuccessful.

The effect of mutation on an aggregation-prone protein: An in vivo, in vitro, and in silico analysis.

Aggregation of initially stably structured proteins is involved in more than 20 human amyloid diseases. Despite intense research, however, how this class of proteins assembles into amyloid fibrils remains poorly understood, principally because of the complex effects of amino acid substitutions on protein stability, solubility, and aggregation propensity. We address this question using ß2-microglobulin (ß2m) as a model system, focusing on D76N-ß2m that is involved in hereditary amyloidosis. This amino acid substitution causes the aggregation-resilient wild-type protein to become highly aggregation prone in vitro, although the mechanism by which this occurs remained elusive. Here, we identify the residues key to protecting ß2m from aggregation by coupling aggregation with antibiotic resistance in E. coli using a tripartite ß-lactamase assay (TPBLA). By performing saturation mutagenesis at three different sites (D53X-, D76X-, and D98X-ß2m) we show that residue 76 has a unique ability to drive ß2m aggregation in vivo and in vitro. Using a randomly mutated D76N-ß2m variant library, we show that all of the mutations found to improve protein behavior involve residues in a single aggregation-prone region (APR) (residues 60 to 66). Surprisingly, no correlation was found between protein stability and protein aggregation rate or yield, with several mutations in the APR decreasing aggregation without affecting stability. Together, the results demonstrate the power of the TPBLA to develop proteins that are resilient to aggregation and suggest a model for D76N-ß2m aggregation involving the formation of long-range couplings between the APR and Asn76 in a nonnative state.

Corrigendum: The Impact of a Dissonance-Based Eating Disorders Intervention on Implicit Attitudes to Thinness in Women of Diverse Sexual Orientations.

[This corrects the article DOI: 10.3389/fpsyg.2019.02611.].

The Impact of a Dissonance-Based Eating Disorders Intervention on Implicit Attitudes to Thinness in Women of Diverse Sexual Orientations.

Dissonance-based body image programs have shown long-term effectiveness in preventing eating disorders and reducing risk factors for eating disorders in women. Here we report on the potential for one such intervention to impact on implicit attitudes toward thinness as well as an explicit measure of eating attitudes, across a sexually diverse group of young women. The Succeed Body Image Programme was adapted to remove heteronormative assumptions and was delivered to a final sample of 56 undergraduate women who reported their sexual orientation as either "predominantly heterosexual" (our term; 1 or 2 on a 7-point Kinsey scale, n = 38) or non-heterosexual (3-7 on the Kinsey scale, n = 18). Before and after the intervention, they completed the Eating Attitudes Test-26, and an associative reaction time task based on the Implicit Association Test, in which bodies of low and higher weight were paired with socially desirable or undesirable traits. A total of 37 predominantly heterosexual women completed a control intervention in which they read NHS leaflets on eating disorders and healthy weight. Results showed that the intervention made predominantly heterosexual participants less prone, versus control, to associating thinness with positive traits on the IAT and all women completing the intervention reported a lower level of disordered eating attitudes at post- than pre-test. Non-heterosexual women, however, showed a non-significant increase in thin-bias on the IAT, perhaps due to their low baseline. These results imply that intensive dissonance-based programs can change attitudes at the automatic, implicit level as well as merely giving women tools to overcome those implicit attitudes.

Restriction Fragment Length Polymorphism-based Genotyping of Toxoplasma gondii from Autopsy-Proven Cases of Acquired Immunodeficiency Syndrome-associated Cerebral Toxoplasmosis.

CONTEXT: Published data on genetic characterization of Toxoplasma gondii (T.gondii) from clinical cases of toxoplasmosis from India is lacking. AIMS: The present study was aimed at identifying genetic types of T. gondii in fatal cases of cerebral toxoplasmosis (CT) associated with HIV, from India. SETTINGS AND DESIGN: Archived tissues of CT were obtained postmortem from 25 acquired immunodeficiency syndrome patients between 2000 and 2014. SUBJECTS AND METHODS: Direct amplification of eight different loci, namely, SAG1, 5'-3'SAG2, Alt. SAG2, SAG3, BTUB, GRA6, C22-8, and L358 followed by restriction fragment length polymorphism was used to genotype the parasite. RESULTS: The canonical Types I, II, or III were not found in our study. More than 96% of the cases harbored atypical genotypes-likely recombinants of the canonical types; one case closely corresponded to Type II genotype. CONCLUSIONS: Thus, a majority of T. gondii causing CT in South India belonged to a noncanonical lineage. These nonarchetypal genotypes differed from the conventional Types I, II, and III and caused devastating severity in patients with CT in the background of HIV. These results are a step further to deciphering the population genetics of this important zoonotic parasitic infection in Indian patients, information that has thus far been lacking.

Genetic characterization of Toxoplasma gondii from autopsy proven cases of AIDS associated cerebral toxoplasmosis in South India.

Toxoplasma gondii (T.gondii) infection can be devastating in the immunodeficient causing high morbidity and mortality. Due to limited availability of both diagnostic facilities and Highly Active Antiretroviral Therapy (HAART), toxoplasmosis continues to be a significant problem amongst Acquired Immuno Deficiency Syndrome (AIDS) patients in India. While scanty literature is available on T. gondii isolates in animals in India, little is known about the genetic diversity of the parasite in humans. Therefore, the present study investigated the genetic diversity of T. gondii in 25 confirmed cases of cerebral toxoplasmosis developing on the background of human immunodeficiency virus (HIV) infection/AIDS. PCR DNA sequencing was performed at four important genetic loci of T. gondii: BTUB, GRA6, alternative SAG2 (alt SAG2) and SAG3 on DNA from tissues obtained at postmortem. The amplified products from all the cases were successfully sequenced except at one locus for one case. Results of the present study suggest that majority of the patients (22/25; 88%) in South India are infected with strains that are recombinants of type II/III and/or strains representing T. gondii different from the archetypal lineages I, II, and III. In addition, clonal types III, MAS, and MAS variant genotypes were encountered. No clonal type I or II was seen in the present study. In addition, variants were observed at alt SAG2 and SAG3 but BTUB and GRA6 were highly conserved. Single nucleotide polymorphisms were observed mainly at two loci which are coding for surface antigens at alt SAG2 and SAG3. In conclusion, the present study reveals genetic diversity in India amongst strains of T. gondii from clinical cases of toxoplasmosis which is in accordance with other recent studies showing a high rate of genetic diversity in this parasite across the globe. There is a need to genotype T. gondii from different forms of toxoplasmosis in humans in India.

The Chennai Declaration: a roadmap to tackle the challenge of antimicrobial resistance.

"A Roadmap to Tackle the Challenge of Antimicrobial Resistance - A Joint meeting of Medical Societies in India" was organized as a pre-conference symposium of the 2 nd annual conference of the Clinical Infectious Disease Society (CIDSCON 2012) at Chennai on 24 th August. This was the first ever meeting of medical societies in India on issue of tackling resistance, with a plan to formulate a road map to tackle the global challenge of antimicrobial resistance from the Indian perspective. We had representatives from most medical societies in India, eminent policy makers from both central and state governments, representatives of World Health Organization, National Accreditation Board of Hospitals, Medical Council of India, Drug Controller General of India, and Indian Council of Medical Research along with well-known dignitaries in the Indian medical field. The meeting was attended by a large gathering of health care professionals. The meeting consisted of plenary and interactive discussion sessions designed to seek experience and views from a large range of health care professionals and included six international experts who shared action plans in their respective regions. The intention was to gain a broad consensus and range of opinions to guide formation of the road map. The ethos of the meeting was very much not to look back but rather to look forward and make joint efforts to tackle the menace of antibiotic resistance. The Chennai Declaration will be submitted to all stake holders.

Response of cricket and spider motion-sensing hairs to airflow pulsations.

Closed-form analytical solutions are presented for the angular displacement, velocity and acceleration of motion-sensing filiform hairs exposed to airflow pulsations of short time duration. The specific situations of interest correspond to a spider intentionally moving towards a cricket, or an insect unintentionally moving towards or flying past a spider. The trichobothria of the spider Cupiennius salei and the cercal hairs of the cricket Gryllus bimaculatus are explored. Guided by earlier work, the spatial characteristics of the velocity field due to a flow pulsation are approximated by the local incompressible flow field due to a moving sphere. This spatial field is everywhere modulated in time by a Gaussian function represented by the summation of an infinite Fourier series, thus allowing an exploration of the spectral dependence of hair motion. Owing to their smaller total inertia, torsional restoring constant and total damping constant, short hairs are found to be significantly more responsive than long hairs to a flow pulsation. It is also found that the spider trichobothria are underdamped, while the cercal hairs of the cricket are overdamped. As a consequence, the spider hairs are more responsive to sudden air motions. Analysis shows that while two spiders of different characteristic sizes and lunge velocities can generate pulsations with comparable energy content, the associated velocity fields display different patterns of spatial decay with distance from the pulsation source. As a consequence, a small spider lunging at a high velocity generates a smaller telltale far-field velocity signal than a larger spider lunging at a lower velocity. The results obtained are in broad agreement with several of the observations and conclusions derived from combined flow and behavioural experiments performed by Casas et al. for running spiders, and by Dangles et al. for spiders and a physical model of spiders lunging at crickets.

Postprandial lipaemia in patients with impaired fasting glucose, impaired glucose tolerance and diabetes mellitus.

AIMS: To compare the postprandial lipid responses in subjects with prediabetes (IFG and IGT), newly detected diabetes mellitus (NDDM) and normal glucose tolerance (NGT). METHODS: Postprandial lipid responses to a standard oral fat challenge was studied in forty-four subjects who were divided after an OGTT into NGT, pure impaired fasting glucose (PIFG), pure impaired glucose tolerance (PIGT) and NDDM. RESULTS: There was a significantly higher postprandial triglyceride (PPTg) response with a higher PPTg area under curve (p=0.004) and peak PPTg levels (p=0.003) in patients with NDDM but not with either PIFG (p>0.05) or PIGT (p>0.05) when compared with NGT. Overall, PPTg responses correlated significantly with fasting plasma glucose (p=0.001) and 2h plasma glucose (p=0.001) but not with age, sex, body mass index, waist, or insulin resistance. CONCLUSION: Subjects with newly detected diabetes mellitus displayed postprandial hypertriglyceridemia after a standard oral fat meal challenge while no such abnormality could be demonstrated in subjects with IFG or IGT. This defect is probably related to glycemic status and insulin resistance.

Primary chondrosarcoma of the breast: a case report.

Mammary sarcomas are uncommon tumors. Of these pure chondrosarcoma without any other area of epithelial or mesenchymal differentiation feature as even rarer. This excludes tumors like malignant cystosarcoma phyllodes and metaplastic carcinoma where malignant cartilaginous areas may be present. Further primary chondrosarcoma arises from breast stroma and not from underlying bone or cartilage. Only five cases of pure and primary chondrosarcoma have been reported so far. The sixth case is reported here.

Clozapine impact on clinical outcomes and aggression in severely ill adolescents with childhood-onset schizophrenia.

OBJECTIVE: To evaluate the impact of clozapine on aggressive behaviour and clinical outcomes in children and adolescents with schizophrenia or schizoaffective disorder. METHODS: We reviewed the charts of 6 children and adolescents who were admitted consecutively to a long-term care facility for clinical outcomes, including seclusion and restraints incidents prior to and during clozapine treatment. We also present a representative case history. RESULTS: We noted clinically significant improvements in social interaction and decreases in the number of violent episodes and homicidal or suicidal thoughts. The global assessment of functioning (GAF) scores improved significantly. Weight gain was significant. CONCLUSIONS: These cases illustrate the benefits of clozapine treatment in refractory childhood-onset schizophrenia. Outcomes are similar to those described in adults. Even though open data limit conclusions from this study, it is pertinent that there was a clinically significant improvement in aggressive behaviours. This may be particularly important for improved morale of patients, their families, and treating staff. It may also be helpful in discharge to a less restrictive environment.

Models for environmentally induced eating disorders: dietary hyperphagia and anorexia nervosa.

The two protocols in this unit provide suggestions for constructing models of eating disorders that are at the opposite ends of the spectrum: dietary hyperphagia and anorexia nervosa. The greatest degree of dietary hyperphagia is induced by giving rats or mice access to a daily choice of highly palatable foods (e.g., chocolate or bread) in addition to their regular chow. Like humans, rats overeat and actually develop physiological requirements for these foods. This model can be used to test the effects of putative anorectic agents on both acute and chronic administration regimens. The second protocol describes a model of compulsive behavior that results in profound weight loss, which is produced by moderate food deprivation along with continuous access to exercise wheels.

Adenocarcinoma renal: factores pronósticos: Experiencia de 10 años del club urológico del sur de la Provincia de Buenos Aires (C.U.S.P.B.A) / Renal adenocarcinoma: prognostic factors: 10 years experience of the Provincia Buenos Aires urologic Club

Objetivo: Establecer factors pronósticos en el adenocarcinoma renal.Material y método: Se realizó un estudio retrospectivo de todos los casos de adenocarcinoma renal operados por los miembros del Club urológico del sur de la provincia de Buenos Aires que participaron en este estudio, entre los años 1986 y 1996. Resultados: Se revisaron 231 casos de carcinoma renal operados en ese período. La edad fue de 62+= 11,63 por ciento fueron hombres. La sobrevida a 5 años fue : 100 por ciento estadio I, 77 por ciento estadio II, 51 por ciento estadio III y, 0 por ciento estadio IV; 32 por ciento fueron incidentales. Los pacientes < de 60 años y los tumores > de 5 cm presentaronmenor sobrevida. Conclusiones: Los tumores de bajo estadio (I y II), < de 5 cm, y en pascientes mayores de 60 años tienen mejor pronóstico en cuanto a la sobrevida global

Interstitial laser hyperthermia, a new method in the management of fibroadenoma of the breast: A pilot study.

BACKGROUND AND OBJECTIVE: This study attempts to evaluate the effect of interstitial laser hyperthermia in breast fibroadenomas as an outpatient procedure. STUDY DESIGN/MATERIALS AND METHODS: In an uncontrolled prospective study, 27 patients younger than 35 years were subjected to laser phototherapy of their breast fibroadenomas. Under real-time ultrasound monitoring, Nd:YAG laser (1,064 nm wavelength) was used at 2 W for 300 sec (600 J) in a continuous wave mode to produce interstitial hyperthermia. Follow-ups were done at 2, 4, and 8 weeks. Subsequently, excision biopsy of residual lumps was performed. RESULTS: There was significant decrease in clinical and sonographic sizes (P < 0.001). Follow-up ultrasound showed a progressive change of hyperechoic texture, from a heterogeneous to a nearly homogeneous one. There were minimal scars (2-3 mm) and no keloid or abscess formation. CONCLUSION: Interstitial laser hyperthermia is a safe, precise, and minimally invasive outpatient procedure for in situ destruction of breast fibroadenomas.

Insuficiencia renal en pacientes nefrectomizados por cancer. Experiencia de diez años del Club Urológico del Sur de la Provincia de Buenos Aires / Renal failure in patients who undergone nephrectomy because of cancer. Experience of CUSPBA

Objetivo: Establecer la incidencia de insuficiencia renal posoperatoria en pacientes nefrectomizados por adenocarcinoma renal. Material y método: Se analizaron retrospectivamente todas las nefrectomías por cancer renal realizadas por los participantes en el estudio entre los años 1986 y 1996. Se evaluó la presencia de insuficiencia renal pre y posoperatoria tomando valores de creatinina de 1,5 mg/dl como límite superior. Se evaluó si hubiera sido posible realizar cirugía conservadora en cada caso. Resultados: se revisaron 231 casos de nefrectomía por cáncer. El 7,8 por ciento presentó insuficiencia reanl posoperatoria (3,9 por ciento transitoria y 3,9 por ciento definitiva) con creatininas>1,5 mg/dl. Sólo el 0,43 por ciento requirió hemodiálisis crónica. La falla renal se presentó a los 18,7 meses posoperatorios. Dentro del grupo donde se hubiera podido efectuar cirugía conservadora, el 2,1 por ciento presentó insuficiencia renal definitiva. Conclusiones: La incidencia de falla renal posnefrectomía es del 7,8 por ciento(3,9 definitiva y 3,9 por ciento transitoria). Sólo el 0,43 por ciento requiere hemodiálisis crónica

Safety and efficacy of ECT in patients with head injury: a case series.

Electroconvulsive therapy (ECT) is a safe and effective treatment for certain psychiatric disorders. Eleven patients who had sustained a closed head injury were treated with ECT for their psychiatric symptoms. Eight patients responded to an index course of ECT: 2 were partial responders and became responders during continuation ECT (cECT); 1 patient was a nonresponder. Post-ECT Neurobehavioral Cognitive Status Examination and Mini-Mental State Examination scores showed no significant decline (P > 0.70 and P > 0.89, respectively) from baseline. Eight patients received cECT, without any lasting cognitive side effects. These findings indicate that ECT can be used effectively in patients with a history of closed head injury, without adverse effects on cognitive functioning.

Breeding preferences of Anopheles culicifacies in the rice agro-ecosystem in Kheda district, Gujarat.

Breeding preferences of Anopheles culicifacies, a principle malaria vector, in the plains of India was studied in the rice agro-ecosystem of Kheda district in central Gujarat. Extensive breeding of this species was found in the rice field channels (20.83 per cent) and in rice fields (5.32 per cent). However, rice nurseries (0.91 per cent) and post-harvested rice fields (2.51 per cent) were less preferred. The species was found in abundance in newly transplanted rice fields and during early months of rice cultivation with a peak prevalence in the non-monsoon (Rabi) season. The breeding of An. culicifacies was inversely proportional and negatively correlated (r = -0.868; p < 0.05) with the height of the plants, whereas it showed a positive correlation (r = 0.779; p < 0.05) with the distance between plants. Rice fields near the villages supported maximum breeding of An. culicifacies (48 per cent) followed by the rice fields, 0.5 to one km away from the human habitation. Co-efficient of association (C8 index) revealed a positive association of the species with An. annularis, An. pallidus, An. subpictus and Cx. quinquefasciatus. However, it was negatively associated with An. nigerrimus, Cx. tritaeniorhynchus and Cx. vishnui sub groups.