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The waste management sector is moving towards sustainable approaches for facilitating resource-recovery possibilities. Agriculture residue (rice straw), cow dung (cattle waste), and clam shells from the ocean are the primary waste materials possessing a huge value addition opportunity. In this study, the effective usage of rice straw and anaerobic sludge from cow dung for bio-energy production was studied. Cow dung was initially anaerobically processed for the generation of biomethane and sludge in a digester for a retention time of 40 days. The anaerobic sludge with rice straw was hydrothermally processed in varying proportions of 1 : 0, 0 : 1, 1 : 1,1 : 2, 2 : 1, 3 : 1, 1 : 3 and temperatures of 240-360 °C for 1 hour with varying biomass loads of 50, 75, 100, 125, and 150 g. Additionally, clam shells, one of the best bioresources, were used as a catalyst in the hydrothermal process at concentrations of 0.2-1 wt%. The maximum bio-oil produced was 36.23 wt% at a temperature of 320 °C, with a biomass load of 100 g, mixed proportion of 2 : 1 and catalyst loading of 0.6 wt%. The produced bio-oil comprised hydrocarbons, aldehydes, and carboxylic acids, as confirmed through GC-MS. In the anaerobic study, ≈0.018 m3 cumulative gas was produced at a retention time of 40 days. The biochar had a higher carbon content and its feasibility for further usage shows promise towards sustainability.
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The Schockley-Quisser (SQ) limit of 28.64% is distant from the Sb2S3 solar cells' record power conversion efficiency (PCE), which is 8.00%. Such poor efficiency is mostly owing to substantial interface-induced recombination losses caused by defects at the interfaces and misaligned energy levels. The endeavor of this study is to investigate an efficient Sb2S3 solar cell structure via accurate analytical modeling. The proposed model considers different recombination mechanisms such as non-radiative recombination, Sb2S3/CdS interface recombination, Auger, SRH, tunneling-enhanced recombination, and their combined impact on solar cell performance. This model is verified against experimental work (Glass/ITO/CdS/Sb2S3/Au) where a good coincidence is achieved. Several parameters effects such as thickness, doping, electronic affinity, and bandgap are scrutinized. The effect of both bulk traps located in CdS and Sb2S3 on the electrical outputs of the solar cell is analyzed thoroughly. Besides, a deep insight into the effect of interfacial traps on solar cell figures of merits is gained through shedding light into their relation with carriers' minority lifetime, diffusion length, and surface recombination velocity. Our research findings illuminate that the primary contributors to Sb2S3 degradation are interfacial traps and series resistance. Furthermore, achieving optimal band alignment by fine-tuning the electron affinity of CdS to create a Spike-like conformation is crucial for enhancing the immunity of the device versus the interfacial traps. In our study, the optimized solar cell configuration (Glass/ITO/CdS/Sb2S3/Au) demonstrates remarkable performance, including a high short-circuit current (JSC) of 47.9 mA/cm2, an open-circuit voltage (VOC) of 1.16 V, a fill factor (FF) of 54%, and a notable improvement in conversion efficiency by approximately 30% compared to conventional solar cells. Beyond its superior performance, the optimized Sb2S3 solar cell also exhibits enhanced reliability in mitigating interfacial traps at the CdS/Sb2S3 junction. This improved reliability can be attributed to our precise control of band alignment and the fine-tuning of influencing parameters.
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Due to ever-increasing global energy demands and dwindling resources, there is a growing need to develop materials that can fulfil the World's pressing energy requirements. Electrochemical energy storage devices have gained significant interest due to their exceptional storage properties, where the electrode material is a crucial determinant of device performance. Hence, it is essential to develop 3-D hierarchical materials at low cost with precisely controlled porosity and composition to achieve high energy storage capabilities. After presenting the brief updates on porous carbons (PCs), then this review will focus on the nitrogen (N) doped porous carbon materials (NPC) for electrochemical supercapacitors as the NPCs play a vital role in supercapacitor applications in the field of energy storage. Therefore, this review highlights recent advances in NPCs, including developments in the synthesis of NPCs that have created new methods for controlling their morphology, composition, and pore structure, which can significantly enhance their electrochemical performance. The investigated N-doped materials a wide range of specific surface areas, ranging from 181.5 to 3709â m2 g-1 , signifies a substantial increase in the available electrochemically active surface area, which is crucial for efficient energy storage. Moreover, these materials display notable specific capacitance values, ranging from 58.7 to 754.4â F g-1 , highlighting their remarkable capability to effectively store electrical energy. The outstanding electrochemical performance of these materials is attributed to the synergy between heteroatoms, particularly N, and the carbon framework in N-doped porous carbons. This synergy brings about several beneficial effects including, enhanced pseudo-capacitance, improved electrical conductivity, and increased electrochemically active surface area. As a result, these materials emerge as promising candidates for high-performance supercapacitor electrodes. The challenges and outlook in NPCs for supercapacitor applications are also presented. Overall, this review will provide valuable insights for researchers in electrochemical energy storage and offers a basis for fabricating highly effective and feasible supercapacitor electrodes.
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Over the past several years, wearable electrophysiological sensors with stretchability have received significant research attention because of their capability to continuously monitor electrophysiological signals from the human body with minimal body motion artifacts, long-term tracking, and comfort for real-time health monitoring. Among the four different sensors, i.e., piezoresistive, piezoelectric, iontronic, and capacitive, capacitive sensors are the most advantageous owing to their reusability, high durability, device sterilization ability, and minimum leakage currents between the electrode and the body to reduce the health risk arising from any short circuit. This review focuses on the development of wearable, flexible capacitive sensors for monitoring electrophysiological conditions, including the electrode materials and configuration, the sensing mechanisms, and the fabrication strategies. In addition, several design strategies of flexible/stretchable electrodes, body-to-electrode signal transduction, and measurements have been critically evaluated. We have also highlighted the gaps and opportunities needed for enhancing the suitability and practical applicability of wearable capacitive sensors. Finally, the potential applications, research challenges, and future research directions on stretchable and wearable capacitive sensors are outlined in this review.
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Dispositivos Eletrônicos Vestíveis , Eletrodos , Humanos , Monitorização Fisiológica , Movimento (Física)RESUMO
Polyimide-silica (PI-Silica) composites are of tremendous research interest as high-performance materials because of their excellent thermal and mechanical properties and chemical resistance to organic solvents. Particularly, the sol-gel method of fabricating such composites is popular for manipulating their properties. In this work, PI-silica composite films are synthesized by the sol-gel method and thermal imidization from the solution mixtures of hydrolyzed tetraethoxysilane (TEOS) (or glycidoxypropyltrimethoxysilane (GPMS)) modified silica and an aromatic polyamic acid (PAA) based on 3,3',4,4'-biphenyl tetracarboxylic dianhydride (BPDA)-p-phenylenediamine (PDA). The phase morphology of composites is found to be controlled by the substitution of TEOS with GPMS. Solid-state NMR spectroscopy is used to confirm the structural components of silica and GPMS-modified silica, whereas FT-IR results confirm the complete imidization of polyimide and composite film and suggest successful incorporation of Si-O-Si bonds into polyimide. The thermal, optical transmittance, and dielectric constant characterizations of pure polyimide and composite films are also carried out. Thermal stability of pure polyimide is found to be increased significantly by the addition of silica, whereas the partial substitution of TEOS with GPMS decreases the thermal stability of the composite, due to the presence of the alkyl organic segment of GPMS. The optical transmittance and dielectric constant of the composite films are controlled by manipulating the GPMS content.
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Here, we report a semiempirical quantum chemistry computational approach to understanding the electrocatalytic reaction mechanism (ERM) of a metallic supramolecular polymer (SMP) with nitrite through UV/vis spectral simulations of SMP with different metal oxidation states before and after interactions with nitrite. In one of our recent works, by analyzing the electrochemical experimental data, we showed that computational cyclic voltammetry simulation (CCVS) can be used to predict the possible ERM of heterometallo-SMP (HMSMP) during electrochemical oxidation of nitrite (Islam T.ACS Appl. Polym. Mater.2020, 2( (2), ), 273-284). However, CCVS cannot predict how the ERM happens at the molecular level. Thus, in this work, we simulated the interactions between the repeating unit (RU) of the HMSMP polyNiCo and nitrite to understand how the oxidation process took place at the molecular level. The RU for studying the ERM was confirmed through comparing the simulated UV/vis and IR spectra with the experimental spectra. Then, the simulations between the RU of the polyNiCo and various species of nitrite were done for gaining insights into the ERM. The simulations revealed that the first electron transfer (ET) occurred through coordination of NO2 - with either of the metal centers during the two-electron-transfer oxidation of nitrite, while the second ET followed a ligand-ligand charge transfer (LLCT) and metal-ligand charge transfer (MLCT) pathway between the NO2 species and the RU. This ET pathway has been proposed by analyzing the transition states (TSs), simulated UV/vis spectra, energy of the optimized systems, and highest occupied molecular orbital-lowest occupied molecular orbital (HOMO-LUMO) interactions from the simulations between the RU and nitrite species.
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The title hemihydrate, C12H9N5·0.5H2O, was isolated from the condensation reaction of quinoline-2-carbaldehyde with 4-amino-4H-1,2,4-triazole. The Schiff base mol-ecule adopts an E configuration about the C=N bond and is approximately planar, with a dihedral angle between the quinoline ring system and the 1,2,4-triazole ring of 12.2â (1)°. In the crystal, one water mol-ecule bridges two Schiff base mol-ecules via O-Hâ¯N hydrogen bonds. The Schiff base mol-ecules are inter-connected by π-π stacking inter-actions [centroid-centroid distances of 3.7486â (7) and 3.9003â (7)â Å] into columns along [10].
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In this study, conjugated conducting polyaniline was fabricated onto titania nanoparticles (PANI-TiO2 NPs) using a microwave-accelerated reaction system. The synthesized nanoparticles were characterized using the techniques of electron microscopy (e.g., FE-SEM and TEM), X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), and ultraviolet-visible (UV-Vis) spectrometry. An ultrasensitive sensor using the electrochemical (I-V) approach was fabricated using a thin film of PANI-TiO2 NPs on a glassy carbon electrode (GCE), and it was found to be selective towards 1,2-diaminobenzene (1,2-DAB) in a buffer phase. From current versus concentration studies, the calibration curve was plotted to estimate the sensor's analytical parameters. The highest sensitivity (19.8165 µA µM-1 cm-2) and lowest detection limit (0.93 ± 0.05 pM) were obtained from the electrochemical assessment by applying a signal-to-noise ratio of 3. A linear calibration plot was attained over a large range of concentration (LDR: 1.0 pM to 0.01 mM). The selective 1,2-DAB sensor was found to be efficient and reproducible in performance, yielding significant results with a fast response time (12.0 s). Therefore, the overall results of the 1,2-DAB chemical sensor suggest that this detection approach might be an easy way to develop an efficient electrochemical sensor for the protection of the environment as well as for use in the healthcare field on a broad scale.
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Limited blood supply and rapid tumor metabolism within solid tumors leads to nutrient starvation, waste product accumulation and the generation of pH gradients across the tumor mass. These environmental conditions modify multiple cellular functions, including metabolism, proliferation, and drug response. However, capturing the spatial metabolic and phenotypic heterogeneity of the tumor with classic in vitro models remains challenging. Thus, in this work a microfluidic tumor slice model was developed to study cell behavior under metabolic starvation gradients. The presented microdevice comprises a central chamber where tumor cells were cultured in a 3D collagen hydrogel. A lumen on the flank of the chamber was used to perfuse media, mimicking the vasculature. Under these circumstances, tumor cell metabolism led to the generation of viability, proliferation and pH gradients. The model decoupled the influence of oxygen from other nutrients, revealing that cell necrosis at the core of the model could be explained by nutrient starvation. The microdevice can be disassembled to retrieve the cells from the desired locations to study molecular adaptions due to nutrient starvation. When exposed to these pH gradients and low nutrient conditions, cancer cells showed multiple changes in their gene expression profile depending on their distance from the lumen. Those cells located further from the lumen upregulated several genes related to stress and survival response, whereas genes related to proliferation and DNA repair were downregulated. This model may help to identify new therapeutic opportunities to target the metabolic heterogeneity observed in solid tumors.
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Dispositivos Lab-On-A-Chip , Microfluídica/métodos , Linhagem Celular Tumoral , Proliferação de Células , Colágeno/química , Neoplasias do Colo/metabolismo , Neoplasias do Colo/patologia , Fase G1 , Humanos , Hidrogéis/química , Concentração de Íons de Hidrogênio , Microfluídica/instrumentação , Oxigênio/análise , TranscriptomaRESUMO
Heterogeneous populations within a tumor have varying metabolic profiles, which can muddle the interpretation of bulk tumor imaging studies of treatment response. Although methods to study tumor metabolism at the cellular level are emerging, these methods provide a single time point "snapshot" of tumor metabolism and require a significant time and animal burden while failing to capture the longitudinal metabolic response of a single tumor to treatment. Here, we investigated a novel method for longitudinal, single-cell tracking of metabolism across heterogeneous tumor cell populations using optical metabolic imaging (OMI), which measures autofluorescence of metabolic coenzymes as a report of metabolic activity. We also investigated whether in vivo cellular metabolic heterogeneity can be accurately captured using tumor-derived three-dimensional organoids in a genetically engineered mouse model of breast cancer. OMI measurements of response to paclitaxel and the phosphatidylinositol-3-kinase inhibitor XL147 in tumors and organoids taken at single cell resolution revealed parallel shifts in metaboltruic heterogeneity. Interestingly, these previously unappreciated heterogeneous metabolic responses in tumors and organoids could not be attributed to tumor cell fate or varying leukocyte content within the microenvironment, suggesting that heightened metabolic heterogeneity upon treatment is largely due to heterogeneous metabolic shifts within tumor cells. Together, these studies show that OMI revealed remarkable heterogeneity in response to treatment, which could provide a novel approach to predict the presence of potentially unresponsive tumor cell subpopulations lurking within a largely responsive bulk tumor population, which might otherwise be overlooked by traditional measurements.
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Neoplasias da Mama/diagnóstico por imagem , Neoplasias da Mama/tratamento farmacológico , Organoides/diagnóstico por imagem , Análise de Célula Única , Animais , Antineoplásicos/farmacologia , Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Proliferação de Células/genética , Modelos Animais de Doenças , Feminino , Humanos , Camundongos , Imagem Óptica , Organoides/metabolismo , Quinoxalinas/farmacologia , Sulfonamidas/farmacologia , Microambiente Tumoral/genéticaRESUMO
In this study, conducting polyaniline (PAni) and silicon dioxide (SiO2) nanocomposites (NCs) were synthesized for chemical sensing applications by microwave assisted reaction technique. Facile synthesis and characterization of the PAni-SiO2 nanocomposites were investigated in details and discussed in this report. For the potential application, 1,4-dioxane chemical sensor was fabricated with the PAni-SiO2 nanocomposites deposited onto glassy carbon electrode (GCE). A very thin uniform film was deposited onto GCE with nanocomposite by using conducting 5% nafion binder at room conditions. To evaluate the sensor analytical performances, a calibration plot such as current versus concentration of 1,4-dioxane was drawn and calculated the analytical parameters from the slope of calibration curve. Results are found as sensitivity (0.5934⯵Aµmol-1â¯L-2 cm-2), detection limit (16.0⯱â¯0.8â¯pmolâ¯L-1), and quantification limit (LOQ; 53.3⯱â¯1.5â¯pmolâ¯L-1) in this observation. Considering the linear region in calibration plot, the linear dynamic range of 1,4-dioxane chemical sensor was found (0.12â¯nmolâ¯L-1 â¼ 1.2â¯mmolâ¯L-1). Besides this, the proposed 1,4-dioxane chemical sensor was exhibited good reproducibility, long-term stability, high accuracy in detecting of 1,4-dioxane in real environmental samples. This research is to develop of a selective and an efficient electrochemical sensor. It might be a simple and easy way by applying electrochemical method to ensure the safe and sustainable green environment.
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d-galactosamine (d-GalN) is a well-known hepatotoxic agent that causes liver injury. Conversely, hepatic macrophages play a crucial role in maintaining liver tissue integrity. Macrophage functions were investigated in hepatic lesions induced by a single intraperitoneal injection of d-GalN (800 mg/kg body weight [BW]) in 6-week-old F344 rats. Blood and liver samples were examined at 8 hr and on 1, 2, 3, and 5 days postsingle injection (PSI). Hepatic lesions consisting of degeneration/sporadic foci of coagulation necrosis, inflammatory cell reaction, and reparative fibrosis were seen on PSI days 1 and 2, reflected by significantly increased serum levels of aspartate transaminase and alanine transaminase and upregulation of CD68 M1 (tumor necrosis factor-α, interleukin [IL]-6, and interferon-γ) and CD163 M2 (transforming growth factor-ß1, IL-10, monocyte chemoattractant protein-1, and IL-4) macrophage-related factors. Double immunofluorescence staining on PSI day 2 demonstrated that 82% of hepatic macrophages expressed of CD163/CD68 simultaneously; 65-75% of MHC class II macrophages showed co-expression of CD163 or CD68 and 95% CD204-expressing macrophages reacted to CD163 or CD68. These findings showed that both M1- and M2-macrophages contributed to the development of hepatic lesions induced by d-GalN and provided information about macrophage activation, indicating the importance of analysis of macrophage phenotypes for hepatotoxicity based on M1/M2-polarization.
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Doença Hepática Induzida por Substâncias e Drogas/etiologia , Galactosamina/toxicidade , Fígado/efeitos dos fármacos , Ativação de Macrófagos/efeitos dos fármacos , Macrófagos/efeitos dos fármacos , Animais , Antígenos CD/genética , Antígenos de Diferenciação Mielomonocítica/genética , Apoptose/efeitos dos fármacos , Doença Hepática Induzida por Substâncias e Drogas/imunologia , Doença Hepática Induzida por Substâncias e Drogas/patologia , Citocinas/genética , Fígado/imunologia , Fígado/patologia , Testes de Função Hepática , Ativação de Macrófagos/imunologia , Macrófagos/imunologia , Macrófagos/patologia , Masculino , Necrose , Ratos Endogâmicos F344 , Receptores de Superfície Celular/genéticaRESUMO
Nitric oxide (NO) is both an important regulatory molecule in biological systems and a toxic xenobiotic. Its oxidation products react with sulfhydryl groups and either nitrosylate or oxidize them. The aerobic reaction of NO supplied by diethylamine NONOate (DEA-NO) with pig kidney LLC-PK1 cells and Zn-proteins within the isolated proteome was examined with three fluorescent zinc sensors, zinquin (ZQ), TSQ, and FluoZin-3 (FZ-3). Observations of Zn2+ labilization from Zn-proteins depended on the specific sensor used. Upon cellular exposure to DEA-NO, ZQ sequestered about 13% of the proteomic Zn2+ as Zn(ZQ)2 and additional Zn2+ as proteome·Zn-ZQ ternary complexes. TSQ, a sensor structurally related to ZQ with lower affinity for Zn2+, did not form Zn(TSQ)2. Instead, Zn2+ mobilized by DEA-NO was exclusively bound as proteome·Zn-TSQ adducts. Analogous reactions of proteome with ZQ or TSQ in vitro displayed qualitatively similar products. Titration of native proteome with Zn2+ in the presence of ZQ resulted in the sole formation of proteome·Zn-ZQ species. This result suggested that sulfhydryl groups are involved in non-specific proteomic binding of mobile Zn2+ and that the appearance of Zn(ZQ)2 after exposure of cells and proteome to DEA-NO resulted from a reduction in proteomic sulfhydryl ligands, favoring the formation of Zn(ZQ)2 instead of proteome·Zn-ZQ. With the third sensor, FluoZin-3, neither Zn-FZ-3 nor proteome·Zn-FZ-3 was detected during the reaction of proteome with DEA-NO. Instead, it reacted independently with DEA-NO with a modest enhancement of fluorescence.
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Corantes Fluorescentes/metabolismo , Hidrazinas/metabolismo , Doadores de Óxido Nítrico/metabolismo , Óxido Nítrico/metabolismo , Proteoma/metabolismo , Espectrometria de Fluorescência/métodos , Zinco/metabolismo , Animais , Corantes Fluorescentes/análise , Células LLC-PK1 , Metaloproteínas/análise , Metaloproteínas/metabolismo , Compostos Policíclicos/análise , Compostos Policíclicos/metabolismo , Proteoma/análise , Proteômica/métodos , Quinolonas/análise , Quinolonas/metabolismo , Compostos de Sulfidrila/análise , Compostos de Sulfidrila/metabolismo , Suínos , Compostos de Tosil/análise , Compostos de Tosil/metabolismo , Zinco/análiseRESUMO
Groundwater quality is often evaluated using microbial indicators. This study examines data from 12 international groundwater studies (conducted 1992-2013) of 718 public drinking-water systems located in a range of hydrogeological settings. Focus was on testing the value of indicator organisms for identifying virus-contaminated wells. One or more indicators and viruses were present in 37 and 15% of 2,273 samples and 44 and 27% of 746 wells, respectively. Escherichia coli (E. coli) and somatic coliphage are 7-9 times more likely to be associated with culturable virus-positive samples when the indicator is present versus when it is absent, while F-specific and somatic coliphages are 8-9 times more likely to be associated with culturable virus-positive wells. However, single indicators are only marginally associated with viruses detected by molecular methods, and all microbial indicators have low sensitivity and positive predictive values for virus occurrence, whether by culturable or molecular assays, i.e., indicators are often absent when viruses are present and the indicators have a high false-positive rate. Wells were divided into three susceptibility subsets based on presence of (1) total coliform bacteria or (2) multiple indicators, or (3) location of wells in karst, fractured bedrock, or gravel/cobble settings. Better associations of some indicators with viruses were observed for (1) and (3). Findings indicate the best indicators are E. coli or somatic coliphage, although both indicators may underestimate virus occurrence. Repeat sampling for indicators improves evaluation of the potential for viral contamination in a well.
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Presently there is no established cell line or small animal model that allows for the detection of infectious human norovirus. Current methods based on RT-PCR and RT-qPCR detect both infectious and non-infectious virus and thus the conclusions that may be drawn regarding the public health significance of positive findings are limited. In this study, PMA RT-PCR and RT-qPCR assays were evaluated for selective detection of infectious poliovirus, murine norovirus (MNV-1), and Norwalk virus. Viruses were inactivated using heat, chlorine, and ultraviolet light (UV). Infectious and non-infectious viruses were treated with PMA before RT-PCR and RT-qPCR. PMA RT-PCR was able to differentiate selectively between infectious and heat and chlorine inactivated poliovirus. PMA RT-PCR was able to differentiate selectively between infectious and noninfectious murine norovirus only when inactivated by chlorine. However, PMA RT-PCR could not differentiate infectious Norwalk virus from virus particles rendered non-infectious by any treatment. PMA RT-PCR assay was not able to differentiate between infectious and UV inactivated viruses suggesting that viral capsid damage may be necessary for PMA to enter and bind to the viral genome. PMA RT-PCR on naked MNV-1 and Norwalk virus RNA suggest that PMA RT-PCR can be used to detect intact, potentially infectious MNV-1 and Norwalk viruses and can be used to exclude the detection of free viral RNA by PCR assay.
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Azidas/farmacologia , Enterovirus/efeitos dos fármacos , Enterovirus/genética , Norovirus/efeitos dos fármacos , Norovirus/genética , Propídio/análogos & derivados , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Animais , Infecções por Caliciviridae/diagnóstico , Infecções por Caliciviridae/virologia , Infecções por Enterovirus/diagnóstico , Infecções por Enterovirus/virologia , Humanos , Propídio/farmacologia , RNA Viral/genética , Reação em Cadeia da Polimerase em Tempo Real/métodos , Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos , Ensaio de Placa Viral , Inativação de Vírus/efeitos dos fármacos , Inativação de Vírus/efeitos da radiaçãoRESUMO
The U.S. Environmental Protection Agency's information collection rule requires the use of 1MDS electropositive filters for concentrating enteric viruses from water, but unfortunately, these filters are not cost-effective for routine viral monitoring. In this study, an inexpensive electropositive cartridge filter, the NanoCeram filter, was evaluated for its ability to concentrate enteroviruses and noroviruses from large volumes of water. Seeded viruses were concentrated using the adsorption-elution procedure. The mean percent retention of seeded polioviruses by NanoCeram filters was 84%. To optimize the elution procedure, six protocols, each comprising two successive elutions with various lengths of filter immersion, were evaluated. The highest virus recovery (77%) was obtained by immersing the filters in beef extract for 1 minute during the first elution and for 15 min during the second elution. The recovery efficiencies of poliovirus, coxsackievirus B5, and echovirus 7 from 100-liter samples of seeded tap water were 54%, 27%, and 32%, respectively. There was no significant difference in virus recovery from tap water with a pH range of 6 to 9.5 and a water flow rate range of 5.5 liters/min to 20 liters/min. Finally, poliovirus and Norwalk virus recoveries by NanoCeram filters were compared to those by 1MDS filters, using tap water and Ohio River water. Poliovirus and Norwalk virus recoveries by NanoCeram filters from tap and river water were similar to or higher than those by the 1MDS filters. These data suggest that NanoCeram filters can be used as an inexpensive alternative to 1MDS filters for routine viral monitoring of water.
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Enterovirus/isolamento & purificação , Filtração/métodos , Norovirus/isolamento & purificação , Virologia/métodos , Microbiologia da Água , Concentração de Íons de Hidrogênio , RNA Viral/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Estados UnidosRESUMO
A study was conducted to examine the role of aquatic plants used in constructed wetlands on the survival of enteric bacteria and viruses. Four small-scale wetland systems, receiving fresh water and two other wetland systems, receiving secondary unchlorinated sewage were used in this study. Fresh water and secondary sewage without the presence of any aquatic plants were used as controls. Escherichia coli, Salmonella typhimurium, bacteriophage MS-2 and poliovirus were added to the waters collected from the wetlands and controls. The presence of aquatic plants significantly increased the die-off of both bacteria in fresh water and secondary sewage. No significant difference in the die-off of E. coli and S. typhimurium was observed in water from wetlands with different types of plants in freshwater. However, there was a significant difference in the die-off of E. coli in water with aquatic plants when sewage was used. The presence of the plants significantly increased the inactivation of MS-2 and poliovirus. Additional work on the survival of E. coli indicated that the plausible mechanism of bacterial die-off in constructed wetlands is through increased microbial competition or predation.
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Escherichia coli/crescimento & desenvolvimento , Levivirus/crescimento & desenvolvimento , Plantas , Poliovirus/crescimento & desenvolvimento , Salmonella typhimurium/crescimento & desenvolvimento , Microbiologia da Água , Áreas Alagadas , Humanos , Esgotos/microbiologia , Esgotos/virologia , Poluição da ÁguaRESUMO
Sedimentation is thought to be one of the mechanisms of microbial reduction from wetlands used for wastewater treatment. This study compared the occurrence and survival of enteric indicator microorganisms and pathogens in the water column and sediments of two constructed surface flow wetlands in Arizona. On a volume/wet weight basis the concentration of fecal coliforms and coliphage in the water column and sediment was similar. However, on a volume/dry weight basis the numbers were one to two orders of magnitude higher in the sediment. Giardia cyst and Cryptosporidium oocyst concentrations were one to three orders of magnitude greater in the sediment compared to the water column. The die-off rates of all the bacteria and coliphage were greater in the water column than the sediment. The die-off rates of fecal coliforms in the water and sediment were 0.256log(10)day(-1) and 0.151log(10)day(-1), respectively. The die-off rates of Salmonella typhimurium in the water and sediment were 0.345log(10)day(-1) and 0.312log(10)day(-1), respectively. The die-off rates of naturally occurring coliphage in water column and sediment were 0.397log(10)day(-1) and 0.107log(10)day(-1), respectively, and the die-off rates of and PRD-1 in water and sediment were 0.198log(10)day(-1) and 0.054log(10)day(-1), respectively. In contrast Giardia die-off in the sediment was greater compared to the water column. The die-off rates of Giardia in water and sediment were 0.029log(10)day(-1) and 0.37log(10)day(-1), respectively. Coliphage survived the longest of any group of organisms in the sediment and the least in the water column. In contrast Giardia survived best in the water column and least in the sediment.
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Cryptosporidium/isolamento & purificação , Enterobacteriaceae/isolamento & purificação , Giardia/isolamento & purificação , Salmonella typhimurium/isolamento & purificação , Microbiologia da Água , Purificação da Água/métodos , Animais , Cryptosporidium/patogenicidade , Ecossistema , Enterobacteriaceae/patogenicidade , Filtração , Sedimentos Geológicos/análise , Giardia/patogenicidade , Oocistos , Salmonella typhimurium/patogenicidade , Movimentos da ÁguaRESUMO
Noroviruses (NVs) are the most common cause of acute nonbacterial gastroenteritis worldwide. An international workshop of 36 people from 5 countries was held on 20-22 March 2002, in Voorhees, New Jersey, to identify barriers to the development of methods for detection of NVs in water and to develop a multiyear research agenda to coordinate efforts to advance the development of methods for detection of NVs. The workshop focused on 3 areas: (1) collection, concentration, and processing of samples; (2) molecular methods for detection and confirmation of NVs; and (3) the detection of infectious NVs through cell culture, infectivity surrogates, and human volunteers. Twelve research projects were developed by the workshop participants.
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Norovirus/isolamento & purificação , Saúde Pública/educação , Microbiologia da Água , Abastecimento de Água/normas , Animais , Infecções por Caliciviridae/diagnóstico , Infecções por Caliciviridae/prevenção & controle , Europa (Continente) , Gastroenterite/diagnóstico , Gastroenterite/prevenção & controle , Técnicas Genéticas , Humanos , Cooperação Internacional , Avaliação das Necessidades , Norovirus/patogenicidade , Saúde Pública/normas , RNA Viral/análise , Manejo de Espécimes/normas , Estados Unidos , Microbiologia da Água/normasRESUMO
The potential for public health risks associated with intrusion of contaminants into water supply distribution systems resulting from transient low or negative pressures is assessed. It is shown that transient pressure events occur in distribution systems; that during these negative pressure events pipeline leaks provide a potential portal for entry of groundwater into treated drinking water; and that faecal indicators and culturable human viruses are present in the soil and water exterior to the distribution system. To date, all observed negative pressure events have been related to power outages or other pump shutdowns. Although there are insufficient data to indicate whether pressure transients are a substantial source of risk to water quality in the distribution system, mitigation techniques can be implemented, principally the maintenance of an effective disinfectant residual throughout the distribution system, leak control, redesign of air relief venting, and more rigorous application of existing engineering standards. Use of high-speed pressure data loggers and surge modelling may have some merit, but more research is needed.