RESUMO
A binding enzyme-linked immunosorbent assay (ELISA) has been developed for measuring nanogram concentrations of semisynthetic pneumocandin antifungal agents in human plasma. Semisynthetic pneumocandin L-733,560 was conjugated to succinylated hemocyanin by water-soluble carbodiimide and was used as an immunogen to produce polyclonal antibodies in rabbits. Pneumocandins were used to directly coat the wells of a microtiter plate, and quantitation was achieved by using rabbit polyclonal antibodies to pneumocandin L-733,560 and goat anti-rabbit immunoglobulin G conjugated to either alkaline phosphatase or horseradish peroxidase. Maximum binding of L-733,560 and most related analogs to the wells of the microtiter plate was found to occur in the first 5 min of incubation at 4 degrees C. Once bound to the plate, these pneumocandins could not be removed from the plate, either by treatment with 4.0 to 6.0 M urea or by treatment with 4.0 to 6.0 M guanidine hydrochloride for 24 h at 4 degrees C. The binding ELISA is linear with drug concentration and can detect levels of L-733,560 as low as 5 ng/ml in human plasma. The assay is also useful for quantitating plasma levels of related semisynthetic pneumocandins including clinical candidate MK-0991.
Assuntos
Antibacterianos/sangue , Antifúngicos/sangue , Ensaio de Imunoadsorção Enzimática/métodos , Peptídeos Cíclicos/sangue , Peptídeos , Animais , Antibacterianos/imunologia , Antibacterianos/farmacocinética , Anticorpos/imunologia , Especificidade de Anticorpos , Antifúngicos/imunologia , Antifúngicos/farmacocinética , Relação Dose-Resposta Imunológica , Equinocandinas , Humanos , Peptídeos Cíclicos/imunologia , Peptídeos Cíclicos/farmacocinética , CoelhosRESUMO
A soluble enzyme amylopectin synthase (UDP-glucose-alpha 1,4-glucan alpha-4-glucosyltransferase) which transfers glucose from uridine 5'-diphosphate glucose (UDP-glucose) to a primer to form alpha-1,4-glucosyl linkages has been identified in the extracts of unsporulated oocysts of Eimeria tenella. UDP-glucose and not ADP-glucose was the most active glucosyl donor. Corn amylopectin, rabbit liver glycogen, oyster glycogen and corn starch served as primers; the latter two were less efficient. The enzyme has an apparent pH optimum of 7.5 and exhibited typical Michaelis-Menten kinetics with dependence on both the primer and substrate concentrations. The Michaelis constants (Km), with respect to UDP-glucose, was 0.5 mM; and 0.25 mg/ml and 1.25 mg/ml with respect to amylopectin and rabbit liver glycogen. The product formed by the reaction was predominantly a glucan containing alpha-1,4 linkages. The specificity of the enzyme suggests that this enzyme is similar to glycogen synthase in eukaryotes and has been designated as amylopectin synthase (UDP-glucose-alpha-1,4-glucosetransferase EC 2.4.1.11).
Assuntos
Amilopectina/biossíntese , Eimeria tenella/enzimologia , Glicogênio Sintase/metabolismo , Animais , Galinhas , Glucose/metabolismo , Concentração de Íons de Hidrogênio , Cinética , Coelhos , Especificidade por Substrato , Temperatura , VerdurasRESUMO
A cDNA clone, SO7', from an Eimeria tenella cDNA library was inserted into the high-expression vector pJC264 and was expressed in Escherichia coli as a fusion protein, CheY-SO7', with a molecular mass of approximately 36 kDa. By using the purified recombinant antigen to immunize young chicks, it was demonstrated that a single dose, without adjuvant, not only protected against severe coccidiosis induced by infection with E. tenella but also protected chicks challenged with the heterologous species Eimeria acervulina, E. maxima, and E. necatrix. By using rabbit antiserum raised against recombinant CheY-SO7', Western blot (immunoblot) analysis of sporulated oocysts of all seven major species of chicken coccidia showed that all species tested contained proteins characteristic of the B class of antigens, of which CheY-SO7' is representative. It seems likely that a single B antigen could protect chickens against severe coccidiosis caused by infection with any of these Eimeria species. Although chicks exposed to prolonged, natural infection develop antibodies to B antigen, active immunization of young chicks with a protective dose of CheY-SO7' does not elicit a humoral antibody response, suggesting that the partial protection results from cell-mediated effector mechanisms. In addition, the cross-protective nature of the immunity indicates that the response to B antigen is different from that induced by natural infection, which elicits a species-specific immunity. To date, the protection induced by B antigen immunization, although remarkable for a single recombinant protein, is not sufficient to compete with prophylactic chemotherapy.
Assuntos
Antígenos de Protozoários/imunologia , Galinhas , Coccidiose/veterinária , Eimeria/imunologia , Doenças das Aves Domésticas/prevenção & controle , Proteínas Recombinantes de Fusão/imunologia , Animais , Anticorpos Antiprotozoários/análise , Western Blotting , Galinhas/parasitologia , Coccidiose/prevenção & controle , Reações Cruzadas , Imunização , Proteínas Recombinantes de Fusão/isolamento & purificação , Especificidade da Espécie , Vacinas/imunologiaRESUMO
Chickens were partially protected against coccidiosis induced by Eimeria tenella by using extracts prepared from sonicated sporulated oocysts or from sonicated sporozoites. Following gel filtration of either extract, most of the protective antigens were confined to a single pool of proteins in the molecular mass range between 20 and 30 kDa. Further purification of proteins from the protective pool of sporulated oocyst extract yielded a protective polypeptide with a molecular mass of 26 kDa. An antiserum raised to this pool identified a polypeptide with a molecular mass of 22 kDa in the protective pool from a similarly prepared extract of sporozoites of E. tenella. In subsequent studies, this antiserum was used as an aid in cloning protective polypeptides.
Assuntos
Antígenos de Protozoários/imunologia , Coccidiose/prevenção & controle , Eimeria/imunologia , Animais , Anticorpos Antiprotozoários/imunologia , Antígenos de Protozoários/isolamento & purificação , Western Blotting , Galinhas , Cromatografia em Gel , Eletroforese em Gel de Poliacrilamida , Feminino , Peso Molecular , CoelhosRESUMO
The isolation in our laboratories of several antigens of interest from sporulated oocysts of Eimeria species by bioselective adsorption on matrices containing immobilized antigen-specific immunoglobulins IgG was initially unsuccessful. The preparations serving as source materials for these antigens contained low levels of the zwitterionic sulfobetaine detergent, Zwittergent 3-12. Since usually immunoaffinity processes are carried out in the presence of various detergents, we were surprised, subsequently, to find this detergent to be the cause of the problem in that it prevented antigen-antibody binding. These findings led us to study the potential role of Zwittergent 3-12 as an eluting agent from matrices holding bioselectively adsorbed materials. The results of seven case studies are presented in this paper and include experiments with beta-D-galactosidase adsorbed biospecifically and bioselectively on matrices via either specific antibody or inhibitor analogue. In all cases, Zwittergent 3-12 proved to be an effective desorbing agent.
Assuntos
Betaína/análogos & derivados , Cromatografia de Afinidade , Animais , Antígenos de Protozoários/isolamento & purificação , Western Blotting , Eimeria/imunologia , Eletroforese em Gel de Poliacrilamida , Focalização Isoelétrica , TensoativosRESUMO
Anti-idiotypic antibodies were raised in rabbits against four monoclonal antibodies with specificity for the surface antigenic determinants of Eimeria tenella sporozoites, the infective stage of the coccidial parasite. Two of the monoclonal antibodies (1073 and 15-1) transferred passive protection in chickens against E. tenella infection. The polyclonal anti-idiotype antibody preparations against protective monoclonal antibodies contained specificities for the paratope-associated idiotypes of these monoclonal antibodies, as assessed by the competitive inhibition of binding of the homologous idiotype-anti-idiotype by the sporozoite antigen. Competitive inhibition of binding of homologous idiotype-anti-idiotype by the parasite antigen was not observed when the anti-idiotype antibody preparations against monoclonal antibodies 1546 and 1096 were tested. The anti-idiotype 1073 and 15-1 antibodies functioned as surrogate antigens in vivo when used for vaccination of young chickens, as evidenced by the induction of partial protective immunity against subsequent challenge infection with virulent parasites and induction of antisporozoite antibodies. These data clearly support the view that anti-idiotypic antibodies raised against the paratope-associated idiotypes can mimic pathogen antigens and therefore can provide a possible alternative approach for the vaccination of chickens against coccidiosis.
Assuntos
Anticorpos Antiprotozoários/imunologia , Antígenos de Protozoários/imunologia , Coccidiose/prevenção & controle , Eimeria/imunologia , Idiótipos de Imunoglobulinas/imunologia , Doenças das Aves Domésticas/prevenção & controle , Vacinas/imunologia , Animais , Anticorpos Anti-Idiotípicos/imunologia , Anticorpos Monoclonais/imunologia , Especificidade de Anticorpos , Galinhas , Imunização Passiva , CamundongosAssuntos
Anticorpos Anti-Idiotípicos/imunologia , Anticorpos Monoclonais/imunologia , Galinhas/imunologia , Eimeria/imunologia , Idiótipos de Imunoglobulinas/imunologia , Animais , Formação de Anticorpos , Especificidade de Anticorpos , Hipersensibilidade Tardia/imunologia , Imunidade Celular , Imunização PassivaRESUMO
Rabbits fed trinitrophenylated bovine serum albumin (TNP-BSA) generated fewer anti-TNP plaque-forming cells but greater numbers of hapten (TNP)-augmentable IgM and IgG PFC following immunization with TNP-Ficoll or TNP-Brucella abortus than did animals not previously fed antigen. Spleen and mesenteric and bronchial lymph nodes were similarly affected. In addition more auto-anti-idiotype (Id) antibody (anti-anti-TNP) was eluted by hapten from spleen cells of antigen-fed rabbits than from spleen cells of control rabbits not prefed antigen. Gel filtration studies ruled out the possibility that the Id binding activity in the eluates was due to immune complexes. The isotype of the anti-Id was IgG except in one rabbit where it was IgM. The results are consistent with the interpretation that the production of auto-anti-Id antibody is one of the factors responsible for the specific depression of the IgM and IgG immune responses which follows antigen feeding. In contrast the antigen feeding resulted in priming for an IgA anti-TNP response without detectable hapten-augmentable IgA PFC.
Assuntos
Anticorpos Anti-Idiotípicos/biossíntese , Antígenos/administração & dosagem , Autoanticorpos/biossíntese , Linfócitos B/metabolismo , Tolerância Imunológica , Idiótipos de Imunoglobulinas/imunologia , Administração Oral , Animais , Linfócitos B/imunologia , Brucella abortus/imunologia , Sistema Livre de Células , Ficoll/análogos & derivados , Ficoll/imunologia , Técnica de Placa Hemolítica , Imunoglobulina A/biossíntese , Imunoglobulina G/biossíntese , Coelhos , Soroalbumina Bovina/imunologia , Baço , Trinitrobenzenos/imunologiaRESUMO
A single-step procedure has been developed to isolate pili from enterotoxigenic Escherichia coli strain B44 of calf origin. Pili, removed from the bacteria by heat shock treatment, were allowed to aggregate at 4 degrees C for 16 h. The precipitated pili, isolated by centrifugation, had typical pili morphology as shown by electron microscopy; ability to bind pig brush border; molecular weight greater than 6 million; and predominance of hydrophobic amino acids. On sodium dodecyl sulfate gel electrophoresis, the subunit pilin migrated as a single polypeptide of molecular weight 17,000.
Assuntos
Fracionamento Celular/métodos , Escherichia coli/ultraestrutura , Fímbrias Bacterianas/ultraestrutura , Adesividade , Aminoácidos/análise , Animais , Antígenos de Bactérias/isolamento & purificação , Eletroforese em Gel de Poliacrilamida , Escherichia coli/imunologia , Fímbrias Bacterianas/análise , Técnicas In Vitro , Intestino Delgado/microbiologia , Microscopia Eletrônica , Microvilosidades/microbiologia , SuínosRESUMO
An antigenic complex has been isolated in a highly purified from from the Melvin strain of Neisseria gonorrhoeae. The complex has a molecular weight of 9.3 x 10(6) and on sodium dodecyl sulfate-polyacrylamide gel electrophoresis was found to consist of several subunits; the most predominant had the following molecular weights: 110,000, 94,000, 68,000, a smear containing (52,000, 48,000, and 44,000), 42,000, 36,000, 29,000, 28,000, 26,000, and 12,000 comprising 89% of the total protein. With the exception of the subunit of molecular weight 110,000, no change in the content or the mobility of other subunits was observed when beta-mercaptoethanol was omitted from the denaturation solution of sodium dodecyl sulfate electrophoresis. Amino acid analysis of the complex showed a predominance of hydrophobic amino acids. These data implicated noncovalent interactions between the subunits. When the cells were labeled with fluorescamine it was possible to obtain a fluorescent complex with identical properties. Among several buffers used for the isolation of the complex, 0.2 M tris(hydroxymethyl)aminomethane buffer (pH 7.5) gave maximum yield with low amounts of lipopolysaccharide and phospholipid; the choice of the buffer for column chromatography did not seem to make any difference. The high protein content and low amounts of lipopolysaccharide and phospholipid are characteristic properties of the complex.
Assuntos
Antígenos de Bactérias/isolamento & purificação , Proteínas de Bactérias/imunologia , Neisseria gonorrhoeae/imunologia , Aminoácidos/análise , Proteínas de Bactérias/isolamento & purificação , Cromatografia de Afinidade , Cromatografia em Gel , Lipopolissacarídeos/análise , Substâncias Macromoleculares , Proteínas de Membrana/imunologiaRESUMO
A lipopolysaccharide was isolated from Neisseria meningitidis group B by phenol/water extraction and purified by differential ultracentrifugation. This preparation exhibited endotoxic properties as shown by the limulus-lysate assay. Mild acid hydrolysis of the lipopolysaccharides yielded a lipid A fraction and a polysaccharide fraction. The lipid A fraction contained fatty acids, phosphorus and glucosamine. Analysis of the polysaccharide fraction revealed the presence of glucose, galactose, glucosamine, 2-keto-3-deoxyoctonic acid and phosphorus. There was no heptose.
Assuntos
Lipopolissacarídeos/análise , Neisseria meningitidis/análise , Ácidos Graxos/análise , Lipídeo A/análise , Monossacarídeos/análiseAssuntos
Hipersensibilidade Tardia , Bainha de Mielina/imunologia , Proteínas do Tecido Nervoso/imunologia , Neurite (Inflamação)/imunologia , Animais , Modelos Animais de Doenças , Cobaias , Imunidade Celular , Ativação Linfocitária , Nervos Periféricos/patologia , Polirradiculopatia/imunologia , Coelhos/imunologia , Nervo Isquiático/imunologia , Testes CutâneosRESUMO
The P0, P1, and P2 proteins were isolated from rabbit sciatic nerve and demonstrated to have molecular weights of 30,000, 18,200, and 12,000, respectively, by polyacrylamide disk gel electrophoresis in the presence of sodium dodecyl sulfate. The P1 protein characterized by peptide mapping, optical rotatory dispersion and encephalitogenic activity appears to be quite similar to the CNS myelin basic protein. The P2 protein is distinctly different from the P1 protein as characterized by peptide mapping and optical rotatory dispersion. It appears to have a distinct secondary structure, predominantly of beta-configuration. The P0 protein is distinctly different from either of the basic proteins, especially with respect to its marked insolubility in aqueous solutions. It contains more than 1.0 mole of hexosamine which is not present in either the P1 or P2 protein. Both the P0 and P2 proteins failed to produce any evidence of experimental allergic encephalomyelitis or neuritis when injected into guinea pigs or monkeys. In contrast, the P1 protein produces experimental allergic encephalomyelitis in both species.
Assuntos
Bainha de Mielina/análise , Proteínas do Tecido Nervoso/isolamento & purificação , Animais , Antígenos , Fenômenos Químicos , Química , Eletroforese Descontínua , Encefalomielite Autoimune Experimental/etiologia , Cobaias , Haplorrinos , Hexosaminas , Hipersensibilidade/etiologia , Métodos , Peso Molecular , Proteínas do Tecido Nervoso/análise , Neurite (Inflamação)/etiologia , Dispersão Óptica Rotatória , Conformação Proteica , Coelhos , Nervo Isquiático/análise , Dodecilsulfato de SódioRESUMO
A 17-residue peptide (Peptide Y) was isolated from the COOH-terminal end of the basic protein of bovine myelin by peptic digestion. This peptide induced experimental allergic encephalomyelitis in the rhesus monkey. Treatment of Peptide Y with cyanogen bromide released three amino acids from the COOH-terminal end and resulted in a tetradecapeptide (Peptide M) which was also encephalitogenic in the rhesus monkey. The sequence of Peptide M is: Phe-Lys-LEU-Gly-Gly-Arg-Asp-Ser-Arg-Ser-Gly-Ser-Pro-Met. Thus a major disease-inducing site active in the rhesus monkey is contained within a 14-residue peptide localized near the COOH-terminal end of the protein. This peptide differs markedly in location and sequence from the 9-residue peptide shown to contain the encephalitogenic determinant for the guinea pig.