An effective nano drug delivery and combination therapy for the treatment of Tuberculosis.

Drug resistance in tuberculosis is exacerbating the threat this disease is posing to human beings. Antibiotics that were once effective against the causative agent, Mycobacterium tuberculosis (Mtb), are now no longer usable against multi- and extensively drug-resistant strains of this pathogen. To address this issue, new drug combinations and novel methods for targeted drug delivery could be of considerable value. In addition, studies have shown that the use of the antidepressant drug fluoxetine, a serotonin reuptake inhibitor, can be useful in the treatment of infectious diseases, including bacterial infections. In this study, an isoniazid and fluoxetine-conjugated multi-walled carbon nanotube nanofluid were designed to increase drug delivery efficiency alongside eliminating drug resistance in vitro. The prepared nanofluid was tested against Mtb. Expression levels of inhA and katG mRNAs were detected by Real-time PCR. ELISA was applied to measure levels of cytokine secretion (TNF-α, and IL-6) from infected macrophages treated with the nano delivery system. The results showed that these nano-drug delivery systems are effective for fluoxetine at far lower doses than for free drugs. Fluoxetine also has an additive effect on the effect of isoniazid, and their concomitant use in the delivery system can have significant effects in treating infection of all clinical strains of Mtb. In addition, it was found that the expression of isoniazid resistance genes, including inhA, katG, and the secretion of cytokines TNFα and IL6 under the influence of this drug delivery system is well regulated. It was shown that the drug conjugation can improve the antibacterial activity of them in all strains and these two drugs have an additive effect on each other both in free and conjugated forms. This nano-drug delivery method combined with host targeted molecules could be a game-changer in the development of a new generation of antibiotics that have high therapeutic efficiencies, low side effects, and the potential to overcome the problem of drug resistance.

Effect of ZnO-based nanophotocatalyst on degradation of aniline.

In this research, a zinc oxide/copper oxide/graphene oxide (ZnO/CuO/GO) nanophotocatalyst was synthesized for photodegradation of aniline as a pollutant, upon exposure to ultraviolet light (UV). Three variables including initial aniline concentration, the nanophotocatalyst dosage, and pH were designed. The statistical test and optimal conditions were determined. The consequences specified that the optimum values of pH, initial aniline concentration, the dosage of nanophotocatalyst, and the reaction time were 6, 150 ppm, 1 g/L, and 3 h, respectively. The obtained results revealed that the photodegradation of aniline was enhanced with doping zinc oxide and CuO on the graphene oxide. Under optimal conditions, 97% photodegradation of aniline was observed. The mechanism of aniline degradation with nanophotocatalyst was evaluated by molecular dynamic (MD) graphs. The interactions between nanophotocatalysts and aniline were considered by energy, density graph.

The Applications of Carbon Nanotubes in the Diagnosis and Treatment of Lung Cancer: A Critical Review.

The importance of timely diagnosis and the complete treatment of lung cancer for many people with this deadly disease daily increases due to its high mortality. Diagnosis and treatment with helping the nanoparticles are useful, although they have reasonable harms. This article points out that the side effects of using carbon nanotube (CNT) in this disease treatment process such as inflammation, fibrosis, and carcinogenesis are very problematic. Toxicity can reduce to some extent using the techniques such as functionalizing to proper dimensions as a longer length, more width, and greater curvature. The targeted CNT sensors can be connected to various modified vapors. In this regard, with helping this method, screening makes non-invasive diagnosis possible. Researchers have also found that nanoparticles such as CNTs could be used as carriers to direct drug delivery, especially with chemotherapy drugs. Most of these carriers were multi-wall carbon nanotubes (MWCNT) used for cancerous cell targeting. The results of laboratory and animal researches in the field of diagnosis and treatment became very desirable and hopeful. The collection of researches summarized has highlighted the requirement for a detailed assessment which includes CNT dose, duration, method of induction, etc., to achieve the most controlled conditions for animal and human studies. In the discussion section, 4 contradictory issues are discussed which are invited researchers to do more research to get clearer results.

Role of Nanofluids in Drug Delivery and Biomedical Technology: Methods and Applications.

Recently, suspensions of several nanoparticles or nanocomposites have attained a vast field of application in biomedical research works in some specified conditions and clinical trials. These valuable suspensions, which allow the nanoparticles to disperse and act in homogenous and stable media, are named as nanofluids. Several studies have introduced the advantages of nanofluids in biomedical approaches in different fields. Few review articles have been reported for presenting an overview of the wide biomedical applications of nanofluids, such as diagnosis and therapy. The review is focused on nanosuspensions, as the nanofluids with solid particles. Major applications are focused on nanosuspension, which is the main type of nanofluids. So, concise content about major biomedical applications of nanofluids in drug delivery systems, imaging, and antibacterial activities is presented in this paper. For example, applying magnetic nanofluid systems is an important route for targeted drug delivery, hyperthermia, and differential diagnosis. Also, nanofluids could be used as a potential antibacterial agent to overcome antibiotic resistance. This study could be useful for presenting the novel and applicable methods for success in current medical practice.

Cyanobacteria as an eco-friendly resource for biofuel production: A critical review.

Cyanobacteria are photosynthetic microorganisms which can be found in various environmental habitats. These photosynthetic bacteria are considered as promising feedstock for the production of the third- and the fourth-generation biofuels. The main subject of this review is highlighting the significant aspects of the biofuel production from cyanobacteria. The most recent investigations about the extraction or separation of the bio-oil from cyanobacteria are also adduced in the present review. Moreover, the genetic engineering of cyanobacteria for improving biofuel production and the impact of bioinformatics studies on the designing better-engineered strains are mentioned. The large-scale biofuel production is challenging, so the economic considerations to provide inexpensive biofuels are also cited. It seems that the future of biofuels is strongly dependent to the following items; understanding the metabolic pathways of the cyanobacterial species, progression in the construction of the engineered cyanobacteria, and inexpensive large-scale cultivation of them.

Modeling and optimization of thermal conductivity and viscosity of MnFe2O4 nanofluid under magnetic field using an ANN.

This research investigates the applicability of an ANN and genetic algorithms for modeling and multiobjective optimization of the thermal conductivity and viscosity of water-based spinel-type MnFe2O4 nanofluid. Levenberg-Marquardt, quasi-Newton, and resilient backpropagation methods are employed to train the ANN. The support vector machine (SVM) method is also presented for comparative purposes. Experimental results demonstrate the efficacy of the developed ANN with the LM-BR training algorithm and the 3-10-10-2 structure for the prediction of the thermophysical properties of nanofluids in terms of the significantly superior accuracy compared to developing the correlation and employing SVM regression. Moreover, the genetic algorithm is implemented to determine the optimal conditions, i.e., maximum thermal conductivity and minimum nanofluid viscosity, based on the developed ANN.

Carbon nanotubes: A review of novel strategies for cancer diagnosis and treatment.

Recently, carbon nanotubes (CNTs) have attracted a lot of attention in the field of cancer diagnosis and therapy. The probability of variable functionalization, compatibility with biological systems, and the thermodynamic properties of CNTs might be the main reasons for such vast investigations. The aim of this study is to review the conducted researches in this field. Studies regarding to the contribution of CNTs in the diagnosis of cancer are reviewed, and the more accurate techniques which need lowest amounts of samples are introduced. In the case of cancer therapy, the studies are categorized in two main classes based on the conducted therapy strategies, including targeted drug delivery systems (DDS), and thermal ablation. In both schemes, use of chemical conjugation is the crucial parts of the studies, which is also emphasized in this review. It is tried to classify the researches based on the cancer type, and introduce the novel strategies developed for both diagnosis and treatment of cancer. The focus is mostly on the biomedical aspect of the methodologies and the subsequent biological responses of the cancer cells to the conducted procedures.

Biomimetics in drug delivery systems: A critical review.

Today, the advanced drug delivery systems have been focused on targeted drug delivery fields. The novel drug delivery is involved with the improvement of the capacity of drug loading in drug carriers, cellular uptake of drug carriers, and the sustained release of drugs within target cells. In this review, six groups of therapeutic drug carriers including biomimetic hydrogels, biomimetic micelles, biomimetic liposomes, biomimetic dendrimers, biomimetic polymeric carriers and biomimetic nanostructures, are studied. The subject takes advantage of the biomimetic methods of productions or the biomimetic techniques for the surface modifications, similar to what accrues in natural cells. Moreover, the effects of these biomimetic approaches for promoting the drug efficiency in targeted drug delivery are visible. The study demonstrates that the fabrication of biomimetic nanocomposite drug carriers could noticeably promote the efficiency of drugs in targeted drug delivery systems.