RESUMO
Immediate-early response 5 (IER5) is a growth factor-inducible protein with homology to the N-terminus of IER2. Deletion analysis shows that a large region of IER5, including the N-terminal region, is involved in cell growth and stress resistance. The N-terminal region mediates IER5 oligomerization and binding to the B55 regulatory subunit of protein phosphatase 2A (PP2A). IER5 physically interacts with the PP2A target proteins ribosomal protein S6 kinase (S6K) and heat shock factor 1 (HSF1), and the interactions are essential for the reduced phosphorylation of S6K and HSF1. Our data indicate that oligomeric IER5 regulates PP2A activity and cell growth.
Assuntos
Proteínas de Ligação a DNA/metabolismo , Proteínas Imediatamente Precoces/metabolismo , Proteína Fosfatase 2/metabolismo , Proteínas Quinases S6 Ribossômicas/metabolismo , Fatores de Transcrição/metabolismo , Proliferação de Células , Células HeLa , Fatores de Transcrição de Choque Térmico , Humanos , Proteínas Imediatamente Precoces/química , Fosforilação , Ligação Proteica , Multimerização Proteica , Proteína Fosfatase 2/química , Estrutura Quaternária de Proteína , Subunidades Proteicas/metabolismoRESUMO
Heat shock factor 1 (HSF1) is the master transcriptional regulator of chaperone genes. HSF1 regulates the expression of the immediate-early response gene IER5, which encodes a protein that has roles in the stress response and cell proliferation. Here, we have shown that IER5 interacts with protein phosphatase 2A (PP2A) and its B55 regulatory subunits. Expression of IER5 and B55 in cells leads to HSF1 dephosphorylation and activation of HSF1 target genes. The B55 subunits directly bind to HSF1. These results suggest that IER5 functions as a positive feedback regulator of HSF1 and that this process involves PP2A/B55 and HSF1 dephosphorylation.