Intimate Partner and Sexual Violence, Reproductive Coercion, and Reproductive Health Among American Indian and Alaska Native Women: A Narrative Interview Study.

Background: The disproportionately high prevalence of poor reproductive and sexual health outcomes among American Indian and Alaska Native (AI/AN) women is related to histories of colonization, oppression, and structural racism. Intimate partner violence (IPV) and sexual violence (SV) contribute to these health outcomes. Materials and Methods: Narrative interviews were conducted with AI/AN women from four tribal reservation communities. Interviews explored connections among sexual and reproductive health, IPV, SV, reproductive coercion (RC), and pregnancy experiences as well as women's experiences of healing and recovery. Results: Among the 56 women interviewed (aged 17-55 years, 77% were aged 40 years and younger), all described multiple exposures to violence and highlighted lack of disclosure related to sexuality, childhood abuse, SV, and historical trauma. Access to confidential reproductive health services and contraceptive education was limited. Almost half (45%) reported experiencing RC in their lifetime. Use of substances occurred in both the context of SV and for surviving after exposure to violence. Women underscored the extent to which IPV, SV, and RC are embedded in histories of colonization, racism, and ongoing oppression. Interventions that incorporate AI/AN traditions, access to culturally responsive reproductive health and advocacy services, organizations, and services that have AI/AN personnel supporting survivors, public discussion about racism, abuse, sexuality, and more accountable community responses to violence (including law enforcement) are promising pathways to healing and recovery. Conclusions: Findings may advance understanding of AI/AN women's reproductive health in the context of historical trauma and oppression. Intervention strategies that enhance resiliency of AI/AN women may promote reproductive health.

When psychologists take a stand: Barriers to trauma response services and advocacy for American Indian communities.

American Indians and other Indigenous groups face numerous traumas resulting from civil rights infractions. Often, these infractions began in historical contexts and continue into modern experiences. The Dakota Access Pipeline (DAPL) and subsequent activist response at Standing Rock are a prime example of how movements asserting human rights are met with resistance from dominant majority groups. Generally, this resistance turns violent as police and security responses from the dominant culture escalate despite the peaceful nature of demonstrations. This impacts mental health among oppressed populations. As #NoDAPL progressed, Indigenous psychologists felt moved to "Stand with Standing Rock" by supporting Water Protectors in need of trauma services. This was met with institutional and systemic barriers to treatment and lack of support from key professional organizations. This article addresses barriers faced by mental health professionals providing services on the frontlines and lists potential resolutions including the development of a crisis response team, infrastructure for communication with individuals onsite, culturally congruent healing, community-building, and prayer. The article serves as a case study for trauma provision among American Indian communities in crisis and is a reminder of the resilience and empowerment that occurs when Indigenous peoples from all over the world come together for a shared cause. This case study format can be a guiding example for service providers choosing to take a stand with marginalized communities asserting social change.

Place of Strength: Indigenous Artists and Indigenous Knowledge is Prevention Science.

The Place of Strength (PoS) project represents an effort to Indigenize program evaluation with Indigenous communities by melding art with prevention science. We propose that Native artists as evaluators: (a) opens avenues of communication for Indigenous perspectives; (b) provides opportunities to capture spiritual, relational, and emotional impacts of prevention programming; and (c) maintains Indigenous processes, language, and values at the center of knowledge production. The New Mexico Tribal Prevention Project (NMTPP) funded seven Southwestern tribes to develop substance abuse prevention programs. In response to their expressed negative experiences with evaluation of prevention strategies, NMTPP piloted PoS. PoS shifted systematic knowledge paradigms to Tribal thought, values, and perspectives embodied in art. Art exists in Native communities as a way of documenting lifeways and historical experiences through various cultural forms. We share the process of collaborating with Native artists to document the impact of substance abuse prevention initiatives through their art within a community context. We offer concepts derived from this project as a community psychology model for re-conceptualizing evaluation utilizing Indigenous knowledge.

Community-based participatory research (CBPR): Towards equitable involvement of community in psychology research.

Community-based participatory research (CBPR) answers the call for more patient-centered, community-driven research approaches to address growing health disparities. CBPR is a collaborative research approach that equitably involves community members, researchers, and other stakeholders in the research process and recognizes the unique strengths that each bring. The aim of CBPR is to combine knowledge and action to create positive and lasting social change. With its origins in psychology, sociology, and critical pedagogy, CBPR has become a common research approach in the fields of public health, medicine, and nursing. Although it is well aligned with psychology's ethical principles and research aims, it has not been widely implemented in psychology research. The present article introduces CBPR to a general psychology audience while considering the unique aims of and challenges in conducting psychology research. In this article, we define CBPR principles, differentiate it from a more traditional psychology research approach, retrace its historical roots, provide concrete steps for its implementation, discuss its potential benefits, and explore practical and ethical challenges for its integration into psychology research. Finally, we provide a case study of CBPR in psychology to illustrate its key constructs and implementation. In sum, CBPR is a relevant, important, and promising research framework that may guide the implementation of more effective, culturally appropriate, socially just, and sustainable community-based psychology research. (PsycINFO Database Record (c) 2018 APA, all rights reserved).

Effectiveness of smartphone technologies on glycaemic control in patients with type 2 diabetes: systematic review with meta-analysis of 17 trials.

Patient education and behavioural interventions for self-management of type 2 diabetes mellitus (T2DM) are effective but place demands on manpower resources. This systematic review aimed to investigate the effectiveness of smartphone technologies (STs) for improving glycaemic control among T2DM patients. CENTRAL, MEDLINE, Embase, CINAHL and ScienceDirect were searched through December 2016. Randomized controlled trials comparing STs with usual diabetes care among T2DM patients and reporting change in glycated haemoglobin (HbA1c) level were included. Seventeen trials (2,225 participants) were included. There was a significant reduction in HbA1c (pooled weighted mean difference: -0.51%; 95% confidence interval: -0.71% to -0.30%; p < 0.001), favouring ST intervention. The pooled weighted mean difference was -0.83% in patients with T2DM <8.5 years and -0.22% in patients with T2DM ≥8.5 years, with significant subgroup difference (p = 0.007). No subgroup differences were found among different follow-up durations, trial locations, patients' age, healthcare provider contract time, baseline body mass index and baseline HbA1c. Compared with usual diabetes care, STs improved glycaemic control among T2DM patients, especially for patients at earlier disease stages (duration of diagnosis <8.5 years). STs could be a complement or alternative to labour-intensive patient education and behavioural interventions, but more studies on up-to-date technologies are needed.

Exposure to crises and resiliency of health care workers in Singapore.

BACKGROUND: Health care workers are exposed to various work-related traumatic incidents and crises, so building emotional resiliency is important. AIMS: To examine exposure to work-related crises and resiliency of health care workers in public hospitals in Singapore. METHODS: We sent questionnaires to health care workers in seven public hospitals. Participation was voluntary and anonymous. We asked about mental health training and exposure to work-related and personal crises. We measured resiliency using a pilot 5-point Likert questionnaire reflecting resistance and resilience constructs. RESULTS: We received 496 responses, a response rate of 58%. More than 70% of hospital staff experienced aggression or violence from patients and relatives, and about a third experienced significant personal crises, most commonly interpersonal conflicts. Those with mental health training were twice as likely to be resistant (OR = 1.8, 95% CI 1.2-2.7) and resilient (OR = 1.9, 95% CI 1.3-2.7) and also more likely to have experienced sudden/unexpected patient deaths (OR = 2.7, 95% CI 1.9-4.0) and aggression or violence from patients and relatives (OR = 5.1, 95% CI 3.0-8.7). CONCLUSIONS: Mental health training appears to improve individuals' perception of resistance and resilience. Hospitals should consider providing mental health and crisis intervention training to improve the emotional resiliency of health care workers.

Cryptococcus neoformans activates RhoGTPase proteins followed by protein kinase C, focal adhesion kinase, and ezrin to promote traversal across the blood-brain barrier.

Cryptococcus neoformans is an opportunistic fungal pathogen that causes meningoencephalitis. Previous studies have demonstrated that Cryptococcus binding and invasion of human brain microvascular endothelial cells (HBMEC) is a prerequisite for transmigration across the blood-brain barrier. However, the molecular mechanism involved in the cryptococcal blood-brain barrier traversal is poorly understood. In this study we examined the signaling events in HBMEC during interaction with C. neoformans. Analysis with inhibitors revealed that cryptococcal association, invasion, and transmigration require host actin cytoskeleton rearrangement. Rho pulldown assays revealed that Cryptococcus induces activation of three members of RhoGTPases, e.g. RhoA, Rac1, and Cdc42, and their activations are required for cryptococcal transmigration across the HBMEC monolayer. Western blot analysis showed that Cryptococcus also induces phosphorylation of focal adhesion kinase (FAK), ezrin, and protein kinase C α (PKCα), all of which are involved in the rearrangement of host actin cytoskeleton. Down-regulation of FAK, ezrin, or PKCα by shRNA knockdown, dominant-negative transfection, or inhibitors significantly reduces cryptococcal ability to traverse the HBMEC monolayer, indicating their positive role in cryptococcal transmigration. In addition, activation of RhoGTPases is the upstream event for phosphorylation of FAK, ezrin, and PKCα during C. neoformans-HBMEC interaction. Taken together, our findings demonstrate that C. neoformans activates RhoGTPases and subsequently FAK, ezrin, and PKCα to promote their traversal across the HBMEC monolayer, which is the critical step for cryptococcal brain infection and development of meningitis.

An association between floor vacuuming and dust-mite and serum eosinophil cationic protein in young asthmatics.

UNLABELLED: Although vacuum cleaning is recommended to reduce allergen levels and improve asthma and allergic rhinitis symptoms, some studies suggest it may increase allergen load in homes. We conducted a cross-sectional study to determine if home floor vacuuming was associated with sensitization to dust-mites and cockroaches, and serum eosinophil cationic protein (ECP), a biomarker for atopy, in 102 physician-diagnosed spirometry-confirmed asthmatics. We collected data on floor type, floor cleaning method and frequency, asthma severity, allergy medications used, serum ECP and skin prick tests (SPT) to three dust-mites [Dermatophagoides pteronyssisinus (Der p), Dermatophagoides farinae (Der f) and Blomia tropicalis (Blo t)] and two cockroaches [Periplanata americana (Per a) and Blatella germanica (Bla g)]. Those who vacuumed had increased sensitization to three dust-mites [adjusted ORs (95%CI) = Der p: 26.6 (1.8-405.2); Der f: 44.8 (3.2-620.9); Blo t: 14.1 (1.8-108.1)] but not to cockroaches, adjusted for cleaning frequency and other methods of floor cleaning. Subjects who vacuumed their floor had higher levels of serum ECP than those who did not [adjusted median difference (95%CI): 9.4 (1.1-17.7)], adjusted for use of nasal corticosteroids among those with allergic rhinitis. Vacuuming is associated with increased sensitization to dust-mite allergens and higher serum ECP. PRACTICAL IMPLICATIONS: We found an association between floor vacuuming and increased sensitization to dust-mite allergens and higher levels of an atopy biomarker. Current recommendations to use vacuuming to control allergen exposure and allergic conditions may need to be reconsidered until further studies are performed.

Characterization of the recognition and functional heterogeneity exhibited by cytokine-induced killer cell subsets against acute myeloid leukaemia target cell.

The polyclonal cytokine-induced killer (CIK) cells exhibit potent cytotoxicity against a variety of tumour cells including autologous and allogeneic acute myeloid leukaemic (AML) targets. At maturity, three lymphocyte subsets: CD3(-) CD56(+), CD3(+) CD56(-) and CD3(+) CD56(+), constitute the bulk of the CIK cell culture. The CD3(-) CD56(+) subset behaves like classical natural killer (NK) cells where cytotoxicity is potentiated by blocking the human leucocyte antigen Class I molecules in the AML targets. Both the CD3(+) CD56(+) and CD3(+) CD56(-) subsets, though known to kill autologous and allogeneic targets to a comparable degree and therefore non-major histocompatibility complex (MHC)-restricted, nevertheless require the presence of the MHC molecule on the target, which interacts with their CD3-T-cell receptor complex. Although CIK cells are often termed 'NK-like' T cells, we have demonstrated that the well-characterized NK receptors KIR, NKG2C/E, NKG2D and DNAM-1 are not involved in the process of AML recognition for the CD3(+) CD56(-) and CD3(+) CD56(+) subsets. The CD3(+) CD56(+) and CD3(+) CD56(-) subsets express a polyclonal and comparable TCRVbeta repertoire in a Gaussian distribution. The CD3(+) CD56(+) subset kills AML targets more efficiently than its CD3(+) CD56(-) counterpart because of the presence of a higher proportion of CD8(+) cells. The CD3(+) CD56(+) subset comprise more terminally differentiated late effector T cells that bear the CD27(+) CD28(-) or CD27(-) CD28(-) phenotype, with a higher granzyme A content. In comparison, the phenotype of the CD3(+) CD56(-) subset is consistent with early effector T cells that are CD27(+) CD28(+) and CD62L(+), known to be less cytotoxic but possess greater proliferative potential.

Redefining the role of metallothionein within the injured brain: extracellular metallothioneins play an important role in the astrocyte-neuron response to injury.

A number of intracellular proteins that are protective after brain injury are classically thought to exert their effect within the expressing cell. The astrocytic metallothioneins (MT) are one example and are thought to act via intracellular free radical scavenging and heavy metal regulation, and in particular zinc. Indeed, we have previously established that astrocytic MTs are required for successful brain healing. Here we provide evidence for a fundamentally different mode of action relying upon intercellular transfer from astrocytes to neurons, which in turn leads to uptake-dependent axonal regeneration. First, we show that MT can be detected within the extracellular fluid of the injured brain, and that cultured astrocytes are capable of actively secreting MT in a regulatable manner. Second, we identify a receptor, megalin, that mediates MT transport into neurons. Third, we directly demonstrate for the first time the transfer of MT from astrocytes to neurons over a specific time course in vitro. Finally, we show that MT is rapidly internalized via the cell bodies of retinal ganglion cells in vivo and is a powerful promoter of axonal regeneration through the inhibitory environment of the completely severed mature optic nerve. Our work suggests that the protective functions of MT in the central nervous system should be widened from a purely astrocytic focus to include extracellular and intra-neuronal roles. This unsuspected action of MT represents a novel paradigm of astrocyte-neuronal interaction after injury and may have implications for the development of MT-based therapeutic agents.

Does high body mass index increase the risk of miscarriage after spontaneous and assisted conception? A meta-analysis of the evidence.

OBJECTIVE: To investigate the association between obesity and miscarriage. DESIGN: Meta-analysis. SETTING: The Academic Unit of Reproductive and Developmental Medicine, The University of Sheffield, United Kingdom. PATIENT(S): Obese and overweight patients who had miscarriage after spontaneous or assisted conception, compared with patients with a normal body mass index. INTERVENTION(S): A systematic review was conducted for all relevant articles in MEDLINE from 1964 to September 2006 and in EMBASE from 1974 to September 2006, using a combination of the following search terms: obesity/obes*/obes$/BMI, miscarriage/abortion/pregnancy, IVF, clomifene/clomiphene, gonadotrophins/gonadotrop*/gonadotrop$. MAIN OUTCOME MEASURE(S): Pregnancy loss at <20 weeks of gestation. RESULT(S): Sixteen studies were included in the meta-analysis. Patients with a body mass index of > or =25 kg/m(2) had significantly higher odds of miscarriage, regardless of the method of conception (odds ratio, 1.67; 95% confidence interval, 1.25-2.25). Subgroup analysis from a limited number of studies suggested that this group of women may also have significantly higher odds of miscarriage after oocyte donation (odds ratio, 1.52; 95% confidence interval, 1.10-2.09) and ovulation induction (odds ratio, 5.11; 95% confidence interval, 1.76-14.83). There was no evidence for increased odds of miscarriage after IVF-intracytoplasmic sperm injection. CONCLUSION(S): There is evidence that obesity may increase the general risk of miscarriage. However, there is insufficient evidence to describe the effect of obesity on miscarriage in specific groups such as those conceiving after assisted conception.

Treatment effects of Fränkel functional regulator III in children with class III malocclusions.

The purpose of this study was to evaluate the skeletal and dental effects produced by the Fränkel functional regulator III appliance in growing children with Class III malocclusions. Thirty preadolescents (initial mean age, 8.0 +/- 1.2 years; mean treatment duration, 1.3 +/- 0.6 years) treated with the Fränkel functional regulator III appliance were compared with 20 matched untreated Class III controls (initial mean age 8.2 +/- 1.1 years; mean observation period, 1.5 +/- 0.6 years). The treatment effects were mainly from backward and downward rotation of the mandible and linguoversion of the mandibular incisors.

In vitro and in vivo evaluation of a technetium-99m-labeled 2-nitroimidazole (BMS181321) as a marker of tumor hypoxia.

UNLABELLED: The 99mTc-labeled 2-nitroimidazole derivative BMS181321, previously studied in experimental models of myocardial and cerebral ischemia, has been evaluated in single-cell and tumor models. METHODS: Accumulation of BMS181321 was studied in aerobic and hypoxic (<10 PPM O2) suspension cultures of Chinese hamster ovary (CHO) cells at 37 degrees C and the oxygen dependency and stability of the accumulated radioactivity determined. Biodistribution studies of the tracer after intravenous injection in C3H mice bearing three different murine solid tumors were performed noninvasively using a gamma camera, as well as invasively by determining blood and tissue levels of radioactivity from 10 min to 24 hr after injection. RESULTS: Accumulation of BMS181321 in aerobic cells in vitro equilibrated within 5 min at a approximately 10-fold level over the external medium. Hypoxic cells showed a linear increase in radioactivity up to 4 hr for cell densities < or = 1 x 10(6)/ml. At higher cell densities (2-4 x 10(6)/ml) there was substantial depletion of radioactivity from the growth medium and increased alteration in the chemical state of the tracer that remained. Low O2 levels (approximately 40 ppm) inhibited the maximal accumulation rate by 50%. Approximately 30% of radioactivity accumulated under hypoxic conditions remained cell-associated after 24 hr. Following intravenous injection, the tracer rapidly distributed throughout the mouse and was predominately cleared through the hepatobiliary system. Blood levels of radioactivity cleared quickly and plateaued at approximately 4% of the total dose from 2-24 hr. Absolute uptake in the tumors was highest 10 min after injection, and the tumor-to-muscle activity ratios increased and plateaued from 4-8 hr at values of 3.5-4.0. Two drugs which affect blood flow and increase hypoxic cell fraction in these tumors, hydralizine and nitro-L-arginine, significantly increased levels of BMS181321 radioactivity over control levels with minimal effects on normal tissue retention. CONCLUSION: These results suggest BMS181321 or an analog of it will be a useful agent to investigate the status of hypoxia in solid tumors experimentally and potentially in the clinic.

Occluded infrainguinal grafts: when to choose lytic therapy versus a new bypass graft.

BACKGROUND: The published results of thrombolysis for occluded bypass grafts, including a prospective multicenter trial, have been disappointing, prompting many investigators to proceed directly to a new bypass rather than attempt graft salvage. Our series of 61 occluded grafts treated with lytic therapy, however, identified a subgroup that justified attempted graft salvage. METHOD: The grafts consisted of saphenous vein 59% (36/61), other vein (arm or composite vein) 21% (13/61), and polytetrafluoroethylene (PTFE) 29% (13/61). The data analysis was designed to identify the graft subgroup that would benefit from lytic therapy by using cumulative survival analysis techniques with the Wilcoxon (Gehan) test for univariate analysis and Cox proportional hazards model for multivariate analysis. Specific variables examined by univariate analysis were graft age (defined as the interval from initial bypass to graft lysis), graft material, graft type (femoropopliteal versus femorotibial), diabetes, symptoms (claudication versus salvage) and duration of occlusion prior to lysis. RESULTS: Complete lysis was achieved in 72% (44/61) of the grafts, and 86% (38/44) had an underlying stenosis that was treated by percutaneous balloon angioplasty (28/38) or surgery (7/38). Three stenotic outflow lesions were not treated. Cumulative 5-year patency for all 61 grafts was 23% +/- 0.075 (SE). Only graft age < 10 versus > 10 months was significant (P < 0.004) by univariate analysis, and it was also the only significant variable found by multivariate analysis; it indicated a 1.58 increase in relative risk of occlusion for the younger grafts. The combination of a saphenous vein graft that was also > 10 months old resulted in a 45% 5-year patency, compared to 21% for < 10-month-old saphenous vein grafts (P < 0.008). A review of 161 bypass grafts performed at our institution over the past 10 years revealed a 52% 5-year secondary patency in previously bypassed limbs, which varied with graft material (67% saphenous, 50% alternative vein, 31% prosthetic). Lysed graft patency was comparable to that of a second bypass using other veins or PTFE conduit. CONCLUSIONS: In the absence of an intact saphenous vein for a second bypass, thrombolytic therapy is an alternative to surgery.

Research: conception to completion.

UNLABELLED: A lack of theory-based nursing research exists in the area of vascular disease. Nursing research is critical to evaluate the effectiveness of specific nursing interventions with the patient population with peripheral vascular disease. This knowledge will strengthen the scientific basis for nursing practice. For the purpose of facilitating increased participation in nursing research by members of the Society for Vascular Nursing, the authors presented the following article, " RESEARCH: Conception to Completion," at the Tenth Annual Symposium in Chicago. The author's current research entitled, "The Effects of Nurse Follow-Up on Increasing Self Care in Patients With Peripheral Vascular Disease: A Prospective Randomized Study," is used as a prototype to illustrate the conceptual, empiric, and interpretive phases of the research process. As the Society for Vascular Nursing moves into its second decade, members will become more focused on research that will generate new knowledge and validate current practices as vascular specialists. There is a great need for the generation and dissemination of knowledge that will help nurses better understand adaptation to health and illness. From this knowledge must come clinical strategies that assist people to promote their own health or cope with illness and its effects on their lives. Vascular specialists must be at the forefront of this effort. Furthermore, the era of cost containment has resulted in increasing pressure to document what nurses do and the differences they make in the outcomes of patient care. The most important outcome of clinical nursing research is the potential benefit it offers to patients. This article will review the steps in conducting nursing research.

Acanthocheilonema viteae (Dipetalonema viteae) in mice: differences in the relative binding of microfilarial surface-specific antibody may explain the contrasting response phenotypes of BALB/c and C57BL/10.

Experiments were carried out to obtain additional data concerning the role of IgM antibodies, specific for the cuticular surface of the microfilariae (mf) of A. viteae, in clearing microfilaraemia from high- and low-responder mice infected by transplanted adult worms. Although BALB/c mice, which sustain a chronic microfilaraemia, produced IgM mf surface-specific antibodies, the binding to target mf was weak when compared to that of antibodies from the serum of the resistant C57BL/10 mice. Furthermore, antibodies from BALB/c mice were not as efficient as those from C57BL/10 mice in promoting the adherence of immune or control leukocytes to mf in vitro. Evidence is provided to show that mf shed surface bound antibody. Although the results do not establish conclusively the mechanism underlying the contrasting response phenotypes of C57BL/10 and BALB/c mice, they provide support for the involvement of antibody in controlling microfilaraemia and suggest that quantitative and qualitative differences in the amount and affinity of IgM antibody specific for the mf surface, together with the natural tendency of the mf to shed surface bound antibody at 37 degrees C, may combine to allow the former strain to clear microfilaraemia efficiently whilst the latter sustains a chronic infection.

Assessment of patient laboratory data in the acutely ill.

Laboratory test results are a valuable source of information. Nurses need to assess laboratory test results as part of the physical assessment of their patients. Comparison of laboratory test results and changes with abnormal physical findings provides the basis for changes in the nursing care plan. Progressive monitoring of laboratory results and prompt interventions might lessen the seriousness of the health problem. In acute care units, the initial group of laboratory tests serves as a baseline for assessing additional test results. Several reference values should be remembered, particularly the electrolytes (potassium, sodium, and calcium), glucose, BUN, creatinine, and albumin. Specific group profiles assist in identifying and in monitoring the patient's health status. Incorporating laboratory test results into the plan and evaluation of care will result in safer and more effective patient care. Referring to laboratory test findings and comparing them with physical assessment findings are required for the delivery of professional nursing care.

Prenatal sensitisation in experimental filariasis: observations on Acanthocheilonema viteae infections in mice.

The probability that in utero exposure to filarial antigens may influence the outcome of a subsequent infection has been investigated using a laboratory model whereby BALB/c mice are implanted with adult, female Acanthocheilonema viteae in order to generate a high-level, long lasting microfilaraemia. When infected using this procedure, BALB/c and (BALB/c x B10) F1 mice can be defined as susceptible and resistant respectively in terms of the microfilaraemia produced. By using microfilaraemic BALB/c mice as mothers, BALB/c and F1 offspring were exposed to the possibility of in utero infection. The finding of microfilariae in foetal tissues and their presence in the blood of two week old mice confirmed the transplacental transmission of parasites in both cases, BALB/c and F1 progeny born to microfilaraemic mothers failed to support a full infection from the L3 stage; similarly, progeny implanted with female worms were as sus-ceptible and resistant respectively as unexposed BALB/c and F1 controls. Spleen cells from in utero exposed, two week old BALB/c and F1 mice recognised filarial antigens in lymphocyte proliferation assays, as did their microfilaraemic mothers. In Western Blot studies, sera from such mice and from foetal, in utero exposed BALB/c mice recognised the same spectrum of A. viteae antigens as their mothers, which strongly suggests the transplacental transfer of maternal antibody. These results demonstrate that the A. viteae-mouse model may be useful in studying transplacental transmission and prenatal sensitisation in experimental filariasis.