RESUMO
In the biomedical field, silicon-based materials are widely used as implants, biomedical devices, and drug delivery systems. Although these materials show promise for implant technologies and clinical applications, many of them fail to simultaneously possess key properties, such as mechanical stability, biostability, stretchability, cell adhesiveness, biofilm inhibition, and drug delivery ability. Therefore, there is considerable need for the development and improvement of new biomaterials with improved properties. In this context, we describe the synthesis of a new hybrid nanocomposite material that is prepared by incorporating bifunctional nanomaterials onto glass and polydimethylsiloxane surfaces. The results show that our hybrid nanocomposite material is elastic, stretchable, injectable, biostable, has pH-controlled drug delivery ability, and display improved cell adhesion and proliferation and, at the same time, impacted bacterial biofilm formation on the respective surfaces.
Assuntos
Bactérias/metabolismo , Biofilmes , Sistemas de Liberação de Medicamentos , Nanoestruturas/química , Impressão Tridimensional , Silício/química , Aderência Bacteriana/efeitos dos fármacos , Materiais Biocompatíveis , Adesão Celular , Proliferação de Células , Dimetilpolisiloxanos/química , Fibroblastos/metabolismo , Vidro/química , Humanos , Concentração de Íons de Hidrogênio , Luminescência , Nanocompostos , Nylons/química , Próteses e Implantes , Staphylococcus epidermidis/efeitos dos fármacos , Estresse Mecânico , Propriedades de Superfície , Fatores de TempoRESUMO
We have synthesized a series of new fluorescent boron systems 1a-c and 2a-d based on nitrogen (NNN) or nitrogen and oxygen (ONO)-containing tridentate ligands. These novel dyes are characterized by high thermal and chemical stability. They show large Stokes shifts (mostly above 3200 cm(-1)) and quantum yields in solution and in the solid state up to 40%. The easy, modular synthesis facilitates the convenient variation of the axial substituent on the central boron atom, allowing the functionalization of this dye for biochemical use. Introducing a long alkyl chain with a phenyl spacer at this axial position enables the self-assembly of the boron compound 2d to form a fluorescent vesicle, which is able to encapsulate small molecules such as sulforhodamine. Additionally, boron compound 2d was found to serve as a dye for cell imaging since it has the capability of binding to the nuclear membranes of HeLa cells. With phospholipids such as DOPC, giant unilamelar vesicles (GUV) are formed. These results demonstrate the wide applicability of this new boron system in supramolecular and medicinal chemistry.