Nerve sheath tumor in the forearm of a common marmoset (Callithrix jacchus).

Tumors of the skin and subcutaneous tissues are uncommon in marmosets. In this report, we describe the gross, histopathology, and immunohistochemical findings of a nerve sheath tumor that arose in the left forearm of an adult female marmoset (Callthrix jacchus).

Changes in local tissue microenvironment in response to subcutaneous long-acting delivery of tenofovir alafenamide in rats and non-human primates.

Several implantable long-acting (LA) delivery systems have been developed for sustained subcutaneous administration of tenofovir alafenamide (TAF), a potent and effective nucleotide reverse transcriptase inhibitor used for HIV pre-exposure prophylaxis (PrEP). LA platforms aim to address the lack of adherence to oral regimens, which has impaired PrEP efficacy. Despite extensive investigations in this field, tissue response to sustained subcutaneous TAF delivery remains to be elucidated as contrasting preclinical results have been reported in the literature. To this end, here we studied the local foreign body response (FBR) to sustained subdermal delivery of three forms of TAF, namely TAF free base (TAFfb), TAF fumarate salt (TAFfs), and TAFfb with urocanic acid (TAF-UA). Sustained constant drug release was achieved via titanium-silicon carbide nanofluidic implants previously shown to be bioinert. The analysis was conducted in both Sprague-Dawley (SD) rats and rhesus macaques over 1.5 and 3 months, respectively. While visual observation did not reveal abnormal adverse tissue reaction at the implantation site, histopathology and Imaging Mass Cytometry (IMC) analyses exposed a local chronic inflammatory response to TAF. In rats, UA mitigated foreign body response to TAF in a concentration-dependent manner. This was not observed in macaques where TAFfb was better tolerated than TAFfs and TAF-UA. Notably, the level of FBR was tightly correlated with local TAF tissue concentration. Further, regardless of the degree of FBR, the fibrotic capsule (FC) surrounding the implants did not interfere with drug diffusion and systemic delivery, as evidenced by TAF PK results and fluorescence recovery after photobleaching (FRAP).

Implantable niche with local immunosuppression for islet allotransplantation achieves type 1 diabetes reversal in rats.

Pancreatic islet transplantation efficacy for type 1 diabetes (T1D) management is limited by hypoxia-related graft attrition and need for systemic immunosuppression. To overcome these challenges, we developed the Neovascularized Implantable Cell Homing and Encapsulation (NICHE) device, which integrates direct vascularization for facile mass transfer and localized immunosuppressant delivery for islet rejection prophylaxis. Here, we investigated NICHE efficacy for allogeneic islet transplantation and long-term diabetes reversal in an immunocompetent, male rat model. We demonstrated that allogeneic islets transplanted within pre-vascularized NICHE were engrafted, revascularized, and functional, reverting diabetes in rats for over 150 days. Notably, we confirmed that localized immunosuppression prevented islet rejection without inducing toxicity or systemic immunosuppression. Moreover, for translatability efforts, we showed NICHE biocompatibility and feasibility of deployment as well as short-term allogeneic islet engraftment in an MHC-mismatched nonhuman primate model. In sum, the NICHE holds promise as a viable approach for safe and effective islet transplantation and long-term T1D management.

Single-dose Diazepam Administration Improves Pairing Success of Unfamiliar Adult Male Rhesus Macaques (Macaca mulatta).

Social housing is one of the best forms of environmental enhancement for nonhuman primates, and current research into pair compatibility and introduction techniques focuses on improving safety and outcome. The gradual steps method (GS), which is widely used for introducing indoor-housed macaques, involves an initial phase of limited physical contact to allow animals to acclimate to one another prior to full contact. A safer, more efficacious introduction method is needed. The administration of diazepam, a sedating anxiolytic medication, is known to increase affiliative behavior in familiar, socially housed rhesus macaques. We hypothesized that administration of a single dose of diazepam prior to full contact introduction without a protected contact phase would improve the success rate of isosexual introductions of unfamiliar macaques as compared with the success rate of GS. We administered 3.2 mg/kg oral diazepam to 34 adult male rhesus macaques (Macaca mulatta) 30-45 min prior to introduction into full contact. Pairs were deemed successful after 14 consecutive days of compatible full-contact housing. Behavioral data collected during these introductions was compared with data collected on 58 adult males during social introductions using GS. Sixteen of 17 introductions (94%) employing diazepam were successful. This success rate was significantly higher than the 45% success rate of introductions using GS. We also found that a longer duration of single housing and increased age were predictive of pair failure in animals introduced using GS. Our results suggest that diazepam administration prior to full contact introductions increases the success rate of male social introductions.

Tracheal trauma in rhesus macaques (Macaca mulatta).

Tracheal disruption is a previously unreported complication of nonhuman primate social trauma. Two cases were identified in rhesus macaques with subcutaneous emphysema. These cases resolved with medical management and demonstrate that the combined use of radiography and tracheoscopy allows rapid assessment and diagnosis of tracheal trauma in nonhuman primates.