Anticoagulation activity of sulfated carboxymethyl cellulose/Azadirachta indica leaf powder-based bio-composite.

Polymeric bio-composites synthesized via a green approach using natural herbs have fascinating anticoagulant activity due to their eco-friendly and non-toxic behavior towards various physical and chemical actions. Herein, we introduce a simple and eco-friendly approach for the fabrication of a new hybrid type of bio-composite based on sulfated carboxymethyl cellulose (S-CMC) and Azadirachta indica leaf powder (S-CMC/NLP). First, a non-toxic sulfating agent called N(SO3Na)3 was used to modify carboxymethyl cellulose into S-CMC. With an ion exchange capacity of 0.25 meq. g-1, the level of sulfation (%) of S-CMC (modified polysaccharide) was measured to be 12.01%. Three types of S-CMC/NLP bio-composites were developed by varying the concentration of NLP. FE-SEM, EDX, and XRD were used to characterize the structural features of S-CMC/NLP bio-composites. FTIR spectroscopy indicated that the S-CMC/NLP bio-composite possesses COO-, -OH and SO3- groups, suggesting the structural similarity to heparin. In addition, the anticoagulant effect of the S-CMC/NLP bio-composite was investigated using PT and APTT assays. The APTT investigation confirmed that following the intrinsic pathway of the coagulation system, 2-NLP/S-CMC bio-composite dose-dependently (0.045-0.28 mg mL-1) prolonged the time of blood coagulation compared to control (pure plasma). The S-CMC/NLP bio-composite showed its potential as a new, safe, and effective candidate for anticoagulant activity.

Effect of carbon dot-doped Ti-MOF on CMC/Agar film and active packaging application on storage quality of fruits.

Ti-metal organic framework (Ti-MOF) doped with carbon dots (CDs) with enhanced antibacterial potential was synthesized using solvothermal-assisted mechanical stirring and used for the fabrication of CMC/Agar-based active packaging films. The incorporation of CD@Ti-MOF not only improved the tensile strength of the CMC/Agar film by 17.4% but also exhibited strong antioxidant activity with 100% of ABTS and 57.8% of DPPH radical scavenging using 0.64 cm2/mL of CMC/Agar/CD@Ti-MOF film. Furthermore, water vapor permeability, oxygen permeability, and ultraviolet light-blocking ability (95.7% of UV-B and 84.7% of UV-A) were improved significantly. The CMC/Agar/CD@Ti-MOF film showed strong antibacterial activity and could inhibit the progress of E. coli up to 8.2 Log CFU/mL and completely stopped the growth of L.monocytogenes after 12 h of incubation. Additionally, CMC/Agar/CD@Ti-MOF film extended the shelf life of cherry tomatoes preserved at 4 °C and delayed the quality degradation, maintaining the visual aspects of the packaging.

A review of starch/polyvinyl alcohol (PVA) blend film: A potential replacement for traditional plastic-based food packaging film.

In recent years, the development of environmentally friendly packaging materials using biodegradable polymers has emerged as a key challenge for scientists and consumers in response to resource depletion and environmental issues caused by plastic packaging materials. Starch and polyvinyl alcohol (PVA) are being recognized as excellent candidates for producing biodegradable food packaging films. Polymer blending has emerged as a practical approach to overcome the limitations of biopolymer films by developing films with unique properties and enhancing overall performance. This review briefly introduces the molecular structure and properties of starch and PVA, summarizes the common preparation methods and properties of starch/PVA blend films, and focuses on different strategies used to enhance starch/PVA blend films, including nanoparticles, plant extracts, and cross-linking agents. Additionally, this study summarizes the application of starch/PVA blend films as active and smart packaging in food preservation systems. This study demonstrates that starch and PVA blends have potential in manufacturing biodegradable food films with excellent properties due to their excellent compatibility and intermolecular interactions, and can be used as packaging films for a variety of foods to extend their shelf life.

Carboxymethyl cellulose/gelatin film incorporated with eggplant peel waste-derived carbon dots for active fruit packaging applications.

Carbon dots (CDs) were derived using eggplant peel by a hydrothermal approach and incorporated into the carboxymethyl cellulose (CMC) and gelatin (Gel) blend to develop sustainable and functional packaging films for fruit preservation. The CD was uniformly dispersed within the CMC/Gel blend to form a dense and continuous film and fashioned a strong interaction with the polymer chain, increasing the tensile strength of the film by 5.0-16.0 %. Also, with the impregnation of CDs, the UV-blocking potential of the CMC/Gel film was greatly improved to the extent of blocking 94.3 % of UV-B and 72.5 % of UV-A, while the water vapor permeability slightly decreased (by 2.7-5.4 %), and the water contact angle of the film marginally expand (by 6.2-19.1 %). The CMC/Gel film with 3 wt% of CD added depicted strong antioxidant efficacy of 100 % against ABTS and 59.1 % against DPPH and displayed strong antibacterial action that inhibited the progress of Listeria monocytogenes and Escherichia coli by 99.8 %. In addition, when table grapes were packaged using a CMC/Gel composite film containing CD and stored at 4 °C for 24 days, the fruits packed with the composite film maintained excellent external quality and extended the shelf life.

Application of bacteriophages in biopolymer-based functional food packaging films.

Recently, food spoilage caused by pathogens has been increasing. Therefore, applying control strategies is essential. Bacteriophages can potentially reduce this problem due to their host specificity, ability to inhibit bacterial growth, and extend the shelf life of food. When bacteriophages are applied directly to food, their antibacterial activity is lost. In this regard, bacteriophage-loaded biopolymers offer an excellent option to improve food safety by extending their shelf life. Applying bacteriophages in food preservation requires comprehensive and structured information on their isolation, culturing, storage, and encapsulation in biopolymers for active food packaging applications. This review focuses on using bacteriophages in food packaging and preservation. It discusses the methods for phage application on food, their use for polymer formulation and functionalization, and their effect in enhancing food matrix properties to obtain maximum antibacterial activity in food model systems.

Sulfur quantum dots as sustainable materials for biomedical applications: Current trends and future perspectives.

Discovered over a decade ago, sulfur quantum dots (SQDs) have rapidly emerged as a sustainable, safe, and inexpensive quantum material. Sustainably synthesizing SQDs using sublimed sulfur powders, typically produced as waste in industrial petrochemical refining processes, has attracted researchers to use these functional quantum materials in various research fields. SQDs quickly found applications in various research fields, such as electronics, environmental sensing, food packaging, and biomedical engineering. Although low production yields, time-consuming and energy-intensive synthetic methods, and low photoluminescence quantum yield (PLQY) have been some problems, researchers have found ways to improve synthetic methods, develop passivating agents, and systematically modify reaction schemes and energy sources to achieve large-scale synthesis of stable SQDs with high PLQY. Nonetheless, SQDs have succeeded tremendously in biomedical and related applications due to their low toxicity, antibacterial and antioxidant properties, biocompatibility, appropriate cellular uptake, and photoluminescent properties. Although the bioimaging applications of SQDs have been extensively studied, their other reported properties indicate their suitability for use as antimicrobial agents, free radical scavengers, and drug carriers in other biomedical applications, such as tissue regeneration, wound healing, and targeted drug delivery.

Advances in sustainable food packaging applications of chitosan/polyvinyl alcohol blend films.

Researchers are addressing environmental concerns related to petroleum-based plastic packaging by exploring biopolymers from natural sources, chemical synthesis, and microbial fermentation. Despite the potential of individual biopolymers, they often exhibit limitations like low water resistance and poor mechanical properties. Blending polymers emerges as a promising strategy to overcome these challenges, creating films with enhanced performance. This review focuses on recent advancements in chitosan/polyvinyl alcohol (PVA) blend food packaging films. It covers molecular structure, properties, strategies for performance improvement, and applications in food preservation. The blend's excellent compatibility and intermolecular interactions make it a promising candidate for biodegradable films. Future research should explore large-scale thermoplastic technologies and investigate the incorporation of additives like natural extracts and nanoparticles to enhance film properties. Chitosan/PVA blend films offer a sustainable alternative to petroleum-based plastic packaging, with potential applications in practical food preservation.

Carrageenan-based active and intelligent packaging films integrated with anthocyanin and TiO2-doped carbon dots derived from sweet potato peels.

Carrageenan-based sustainable active and pH-dependent color-changing composite films were fabricated by blending anthocyanin extracted from sweet potato peel (SPA) with TiO2-doped carbon dots (Ti-CDs) prepared using the biowaste of SPA extraction. The SPA and Ti-CDs were compatible with the carrageenan matrix and were uniformly dispersed in the used polymer to form a homogeneous film with increased mechanical properties. The composite film added with SPA and 3 wt% Ti-CD showed 100 % UV protection, superb antioxidant (100 % DPPH and ABTS scavenging assay), and potent antibacterial activity (complete eradication of foodborne L.monocytogenes and E. coli strains after 3 h incubation). Additionally, the composite films showed distinguishable colorimetric responses to pH 7-12 buffers and volatile ammonia. The intelligent sensing ability of the composite film was assessed through shrimp freshness monitoring, and the film's hue shifted from pink (fresh shrimp) to yellow/brown (inedible shrimp) during storage. Shrimp packaging studies have shown that composite films retard the rate of food quality change during storage and are a good indicator of shrimp spoilage. Therefore, the designed film is expected to have high applicability as a chip, and quick on-site sensor that detects seafood quality in real-time, and a highly effective multifunctional film for better product quality preservation.

Sustainable packaging film based on cellulose nanofibres/pullulan impregnated with zinc-doped carbon dots derived from avocado peel to extend the shelf life of chicken and tofu.

A cellulose nanofiber (CNF)/pullulan (PUL) based multifunctional composite film was developed for active packaging applications by incorporating Zn-doped avocado-derived carbon dots (Zn-ACDs). The incorporation of Zn-ACDs improved the interfacial compatibility and produced a dense cross-sectional structure of the composite films. The Zn-ACDs added film showed no significant difference in water vapor permeability and surface hydrophilicity compared to the neat CNF/PUL film, but the tensile strength and elongation at break increased by ~45.4 % and ~64.1 %, respectively. The addition of 5 wt% Zn-ACDs to the CNF/PUL matrix resulted in 100.0 % UV blocking properties, excellent antioxidant activity (100.0 % for ABTS and 68.0 % for DPPH), and complete eradication of foodborne pathogens such as Listeria monocytogenes ATCC 15313 and Escherichia coli O157:H7 after 3 h of exposure. The CNF/PUL composite film with Zn-ACDs applied to the active packaging of chicken and tofu significantly reduced the total growth of aerobic microorganisms without significantly changing the actual color of the packaged chicken and tofu for 9 days at 10°C. This study demonstrates that CNF/PUL composite films with Zn-ACDs are a sustainable and environmentally friendly option for protecting food from microbial contamination.