[Application of tissue-marking dyes for pathologic examination of surgical and autopsy specimens]. / Primenenie markirovochnykh krasitelei pri patologo-anatomicheskom issledovanii operatsionnogo materiala.

OBJECTIVE: To assess the applicability of imported tissue-marking dyes and the samples of experimental dyes and decorative acrylic paints to mark the resection margins of a surgical specimen. MATERIAL AND METHODS: Three sets of tissue-marking dyes: 2 imported sets and 1 experimental set, each containing red, orange, yellow, green, blue, purple, and black dyes, and a set of decorative acrylic paints containing black, blue, light blue, green, yellow, ocher, orange, magenta, and purple dyes. The experimental dyes and imported ones were used to mark tonsillar fragments obtained at tonsillectomy. The set of experimental dyes and that of decorative acrylic paints were used to stain the fragments of autopsy specimens (the skeletal muscles, pancreas, and large bowel). The tissues obtained at autopsy were marked before and after fixation in 40% formalin for 30 min and 24 hours. The specimens were subjected to standard tissue processing. Paraffin blocks were cut into 5-µm sections that were stained with hematoxylin and eosin. To estimate resection margin marking, each specimen was examined by 7 researchers who independently assessed the covering ability of a dye and its color in the paraffin block and microslides. RESULTS: All researchers correctly identified purple, black, and green colors from the three sets of dyes in the surgical tonsillar specimen. When examining the autopsy specimens, all the experts correctly recognized magenta and green decorative acrylic paints and black and blue experimental dyes. The time of fixation and the type of tissue did not affect the color of a dye in the paraffin block and tissue specimen. CONCLUSION: Some of experimental dyes and decorative acrylic paints are highly competitive with imported tissue-marking dyes in their characteristics, are correctly recognized in the block and tissue specimen under a microscope, and can be proposed to mark the resection margins of the examined tissues.

[Morphological characteristics of the right gastroepiploic artery and its use in myocardial revascularization]. / Morfologicheskaia kharakteristika pravoi zheludochno-sal'nikovoi arterii i ee ispol'zovanie pri revaskuliarizatsii miokarda.

The results of morphological and morphometrical investigation of the biopsy and autopsy material from 35 right gastroepiploic arteries were analyzed. Histological and histochemical methods were used. The anatomo-topographic and morphometrical characteristics of the right gastroepiploic artery allow it to be used for gastro-coronary shunting. It makes the possibilities of the aorto-arterial revascularization of the myocardium substantially wider.