Effect of basic fibroblast growth factor on angiogenesis and bone regeneration in non-critical-size bone defects in rat calvaria.

We used microcomputed tomography (micro-CT) to evaluate the effects of basic fibroblast growth factor (FGF-2) contained in absorbable collagen sponges on angiogenesis and bone regeneration in rat calvarial non-critical-size bone defects. Two symmetrical non-critical-size calvarial bone defects (diameter, 2.7 mm) were created in male Fisher rats. An absorbable collagen sponge with or without FGF-2 (0.1% or 0.3%) was implanted into each defect. Blood vessel volume and bone volume were calculated using software. On day 28, blood vessel volume and bone volume were significantly greater in the 0.3% and 0.1% FGF-2 groups than in the control group. FGF-2 concentration-dependently increased blood vessels and bone formation in non-critical-size bone defects in rat calvaria.

Effect of a chitosan sponge impregnated with platelet-derived growth factor on bone augmentation beyond the skeletal envelope in rat calvaria.

We evaluated the ability of platelet-derived growth factor (PDGF) to promote bone augmentation beyond the skeletal envelope in rat calvaria. The calvariae of 14 rats were exposed, and two plastic caps-one with 0.03% PDGF and a chitosan sponge and one with a chitosan sponge alone-were placed. Microcomputed tomography and histologic sections were used to determine the amount of bone augmentation within the plastic caps. Bone volume was calculated using measurement software. Bone volume and amount of bone augmentation were significantly greater in the PDGF group than in the control group. In conclusion, a chitosan sponge containing 0.03% PDGF enhanced bone formation beyond the skeletal envelope in rat calvaria.

FGF-2 angiogenesis in bone regeneration within critical-sized bone defects in rat calvaria.

OBJECTIVE: This study examined the effect of basic fibroblast growth factor (FGF)-2 with an absorbable collagen sponge on angiogenesis and bone regeneration in rat calvarial critical-sized bone defects using microcomputed tomography. METHODS: Forty 7-week-old male Fischer rats were used. The symmetrical critical-sized calvarial bone defects (5 mm diameter) were created. An absorbable collagen sponge with or without FGF-2 (0.1% or 0.3%) was implanted into each bone defect. RESULTS: Blood vessel and bone volumes were significantly higher in the 0.3% FGF-2 group compared with the control and 0.1% FGF-2 groups on day 28. Significantly more osteoblast- and osteoclast-like cells were seen in the 0.3% FGF-2 group. CONCLUSIONS: Thus, FGF-2 increased blood vessel and bone formation in rat calvarial critical-sized bone defects.