RESUMO
Introduction: Low back pain is the most common type of chronic pain. We examined pain-related behaviors across 18 weeks in rats that received injury to one or two lumbar intervertebral discs (IVD) to determine if multi-level disc injuries enhance/prolong pain. Methods: Twenty-three Sprague-Dawley adult female rats were used: 8 received disc puncture (DP) of one lumbar IVD (L5/6, DP-1); 8 received DP of two lumbar IVDs (L4/5 & L5/6, DP-2); 8 underwent sham surgery. Results: DP-2 rats showed local (low back) sensitivity to pressure at 6- and 12-weeks post-injury, and remote sensitivity to pressure (upper thighs) at 12- and 18-weeks and touch (hind paws) at 6, 12 and 18-weeks. DP-1 rats showed local and remote pressure sensitivity at 12-weeks only (and no tactile sensitivity), relative to Sham DP rats. Both DP groups showed reduced distance traveled during gait testing over multiple weeks, compared to pre-injury; only DP-2 rats showed reduced distance relative to Sham DP rats at 12-weeks. DP-2 rats displayed reduced positive interactions with a novel adult female rat at 3-weeks and hesitation and freezing during gait assays from 6-weeks onwards. At study end (18-weeks), radiological and histological analyses revealed reduced disc height and degeneration of punctured IVDs. Serum BDNF and TNFα levels were higher at 18-weeks in DP-2 rats, relative to Sham DP rats, and levels correlated positively with remote sensitivity in hind paws (tactile) and thighs (pressure). Discussion: Thus, multi-level disc injuries resulted in earlier, prolonged and greater discomfort locally and remotely, than single-level disc injury. BDNF and TNFα may have contributing roles.
RESUMO
Capillary assembly of liquid particles (CALP) is a microfabrication strategy for engineering arbitrarily shaped polymer colloids. The method entails depositing emulsion particles into patterned microarrays within a fluidic cell: coalescence, polymerization, and extraction of the deposited material engender faceted colloids. Herein, the versatility of CALP is demonstrated by using both consecutive assembly and heterogeneous coassembly to engineer geometrically diverse Janus and patchy colloids. Liquid particles (LPs) can be patterned laterally across the plane of the template by manipulating the capillary immersion force, liquid particle hardness, and rate of coalescence. Bilayers of different polymeric LPs and patchy microarrays are fabricated, comprising solid colloids made from various materials including poly(styrene), p-styryltrimethoxysilane, and iron oxide. Eleven different structures including concentric Janus squares, triblock ellipsoids, and planar tetramer and pentagonal patchy particles are described. All particles are fluorescently labeled, resist flocculation, withstand extended heating, and endure dispersion in organic solvent. Further crystallization and processing into colloid-based microscale devices is therefore anticipated. Heterogeneous CALP combines top-down microfabrication with bottom-up synthesis to engineer nonequilibrium particle structures that cannot be made with wet chemistry. CALP enables the design and fabrication of colloids with complex internal construction to target hierarchical functional materials. Ultimately, the integration of colloidal building blocks comprising multiple components that are independently addressable is crucial for the development of nano/micromaterials such as filtration devices, sensors, diagnostics, solid-state catalysts, and optical electronics.
RESUMO
Capillary assembly is a versatile method for depositing colloidal particles within templates, resulting in nano/microarrays and colloidal superstructures for optical, plasmonic, and sensory applications. Liquid particles (LPs), comprised of oligomerized 3-(trimethoxysilyl)propyl methacrylate, are herein shown to deposit into patterned cavities via capillary assembly. In contrast to solid colloids, LPs coalesce upon solvent evaporation and assume the geometry of the template. Incorporating small molecules such as dyes followed by LP solidification generates fluorescent polymer microarrays of any geometry. The LP size is inversely proportional to the quantity of deposited material and the convexity of the final polymer array. Cavity filling can be tuned by increasing the assembly temperature. Extraction of the polymerized regions produces solidified particles with faceted shapes including square prisms, trapezoids, and ellipsoids with sizes up to 14 µm that retain the shape of the cavity in which they are initially held. LP deposition thus presents a highly controllable fabrication scheme for geometrically diverse polymer microarrays and anisotropic colloids of any conceivable polygonal shape due to space filling of the template. The extension of capillary assembly to LPs that can be doped with small molecule dyes and analytes invaluably expands the synthetic toolbox for top-down, scalable, hierarchically engineered materials.