RESUMO
To better understand the molecular mechanisms related to growth promotion in the early developmental stages of Eustoma grandiflorum (Raf.) Shinn. under end-of-day far-red light (EOD-FR) treatment, we analyzed the leaf transcriptome of treated (EOD) and untreated plants (Cont) by using RNA-seq technology. EOD-FR treatment for only about 2 weeks in regions with limited sunshine during winter resulted in significantly higher internode length between the 3rd and 4th nodes on the main stem in EOD than in Cont. Among the differentially expressed genes (DEGs) related to synthesis or transport of auxin, higher levels of YUCCA (CL6581) and PIN4 (CL6181) were noted after treatment in EOD than in Cont in the leaf. In addition, high expression levels of GA20ox (Unigene11862) related to gibberellin (GA) synthesis and transcription factor bHLH 135 (CL7761) were observed in the stem of EOD, 3 h after treatment. A vertical section of the stem showed that the pith length of cells at the 4th node was longer in EOD than in Cont. Collectively, these results suggested that EOD-FR treatment increased the expression of DEGs related to GA and auxin biosynthesis, bHLH transcription factor, and internodal cell elongation along the longitudinal axis of Eustoma plants.
Assuntos
Regulação da Expressão Gênica de Plantas/efeitos da radiação , Luz , Traqueófitas/crescimento & desenvolvimento , Traqueófitas/genética , Biologia Computacional/métodos , Perfilação da Expressão Gênica , Ontologia Genética , Sequenciamento de Nucleotídeos em Larga Escala , Folhas de Planta/genética , Caules de Planta/genética , Reprodutibilidade dos Testes , TranscriptomaRESUMO
It is very important for advanced cancer patients who receive palliative care to select appropriate places to stay during the end-of-life period. We reviewed the cases of 147 patients who were referred to palliative care services from our hospital. Forty-four patients(30%)were referred to palliative care facilities, and 103 patients(70%)received palliative care in their homes. Fifty-two patients(47%)died at a palliative care facility, 41(37%)died at home, and 18(16%)died at our hospital. The proportion of patients who died at home was three times the number of people who wanted to die at home, according to a Japan National questionnaire. To increase cooperation between regional hospitals and home palliative care services, we attended meetings and conferences conducted by the Nishinomiya City Medical Association. As a result of these activities, we think that the number of patients who die at home has increased.