Analyzing the Influence of Conservation Tillage and Manure on Soil Parameter Modulations in Croplands.

Nowadays, when the human impact on the environment becomes prominent daily, specific steps are needed to mitigate or halt those changes. By far, agricultural land is most affected by the degradation process, leading to soil erosion and decreased soil quality. Sustainable measures are needed to find a solution to that problem. This study, located in an agricultural area in northwestern Croatia, gives an insight into how different tillage systems (conventional and conservation) with the addition of manure will affect soil physicochemical properties, hydrology response, and overall yield. To assess hydrological response, a rainfall simulator was used; meanwhile, soil samples were taken to determine bulk density, soil water content, water-stable aggregates, and soil organic matter. Soil water content did not show significant differences, whereas bulk density and penetration resistance yielded significantly higher values at 15-30 cm depth compared to 0-15 cm depth. Also, the conservation manure recorded lower bulk density and penetration resistance values than conventional tilled treatments. Water-stable aggregates and soil organic matter were severely affected by manure addition and yielded an increase after harvest. Hydrological response was delayed for the treatments with manure addition. Crop yield was also significantly higher for the conventional treatment with manure addition, whereas the control plots had lower yields. The results of this study show the positive aspects of conservation tillage, especially with manure addition, where soil quality is preserved or even increased.

Spatio-temporal monitoring of mercury and other stable metal(loid)s and radionuclides in a Croatian terrestrial ecosystem around a natural gas treatment plant.

The natural gas industry bears a certain contamination risk to human and biota due to, among others, mercury, arsenic, and naturally occurring radioactive material content in gas. We tracked multiple stabile metal(loid)s and radionuclides within the natural gas treatment plant Molve, Croatia, ecosystem during the last decade through a comprehensive monitoring of soil, earthworms, moss, livestock (blood, milk, hair, urine, and feces from cows), and wildlife animals (brain, muscle, liver, and kidney of European hare and pheasant). The level of mercury and other stable metal(loid)s has shown temporal variation, but without an obvious trend. The found spatial differences in soil and earthworms were based on the differing soil characteristics of the sampled locations and exceeded the maximal allowable concentration of arsenic and zinc for agricultural soil. The status of essential copper, selenium, and zinc in cows, hares, and pheasants inclined towards deficiency. The measured stable metal(loid) levels in soil and animal samples were generally in the same range of values reported in earlier decades from the same area or non-polluted areas across Europe. The consumption of local cow and game products (e.g., milk, meat) can be considered safe for human health, although game offal is advised to be avoided as a food item due to the low risk of lead and cadmium's adverse effects. Although the activity concentrations of some radionuclides in moss were higher than reported for pristine areas, transfer from soil to moss was assessed as average (except for lead-210). Radiological risk to human and biota around the gas treatment plant Molve was estimated as negligible.

Agriculture management and seasonal impact on soil properties, water, sediment and chemicals transport in a hazelnut orchard (Croatia).

Soil and water conservation practices are key to agroecosystems sustainability and avoiding diffuse pollution. Here, we compare the impacts of different types of mulch, barley straw (Straw), wooden chips (Chip) and tillage (Till) on vegetation mulch cover (VMC); soil properties, bulk density (BD), mean weight diameter (MWD), water stable aggregates (WSA), soil water content (SWC), soil organic matter (SOM), pH and total phosphorous (P), potassium (K), calcium (Ca), chromium (Cr), manganese (Mn), iron (Fe), nickel (Ni), copper (Cu), Zinc (Zn) and lead (Pb). We also assessed the ponding time (PT), runoff time (RT), runoff, sediment concentration (SC), sediment loss (SL) and chemicals transport (the same studied in soil). A set of rainfall simulation experiments (90 in total) was applied in the different Spring, Summer, and Fall treatments. The results showed that mulch increased VMC in all the seasons, while other properties (BD; MWD, WSA SOM, pH) were not affected, especially in Spring. The biggest impact was observed in Fall, especially in the Till plot, due to the tillage practices applied in Summer. Mulch increased PT, RT and reduced runoff, SL and chemicals transport. Chemical losses were very much associated with SL, and the concentration of P and metals in soil depended on soil Ca and pH. SWC, MWD and runoff were inversely related to PT, RT and SC. Finally, BD, VMC and SOM were highly associated. Overall, tillage practices dramatically impact SL, and diffuse pollution and urgent measures are needed to reverse this. Mulching is excellent and cost-effective to mitigate the impacts of agriculture on land degradation and diffuse pollution.

Temporal impacts of pile burning on vegetation regrowth and soil properties in a Mediterranean environment (Croatia).

Burning slash piles is common in the Mediterranean regions to manage the unwanted biomass. This work aims to study the effect of two experimental pile burns ((moderate (MS) and high severity (HS)) conducted in Croatia on vegetation and soil compared to an unburned treatment (UB). We studied vegetation cover (VC), soil water repellency (SWR), mean weight diameter (MWD), water-stable aggregates (WSA), pH, electrical conductivity (EC), soil organic matter (SOM), the total content of calcium (Ca), aluminium (Al), silicon (Si), and iron (Fe), available phosphorus (P2O5) and potassium (K2O). The impacts of the burn were studied 5 days after burning (DAB), 3, 8, 12, and 15 months after burn (MAB). The results showed that VC was significantly higher in UB than in MS 5 DAB-15MAB. SWR was significantly higher in the smallest soil fractions (<0.25 mm) in burn treatments than in the UB on 5 DAB and 3 MAB. Also, 3 MAB, MWD and WSA were significantly lower in the burned treatments than in UB. pH was significantly higher in HS than in UB 3, 12 and 15 MAB, while EC was significantly higher in HS than in UB, 5 DAB, 3 and 15 MAB. MS and HS did not significantly affect SOM content. Total Ca was significantly higher in burn treatments than in UB 5 DAB-12 MAB. Total Al and Si were significantly higher in UB than in HS (5 DAB-15MAB), while total Fe was significantly higher in UB than in HS 5 DAB, and 8-15 MAB. P2O5 was significantly higher in HS than in UB from 3 to 15 MAB, while K2O was significantly higher in MS and HS than in UB 5 DAB. Soils need more time to recover from pile burning disturbance, likely due to the soil poor natural conditions, therefore this type of management is detrimental.

Carbapenem-Resistant Acinetobacter baumannii Recovered from Swine Manure.

Recently, concerns have been raised about the possibility of Acinetobacter baumannii transmission between animals and humans. So far, A. baumannii has been reported in animals with which people can come into contact. The presence of this pathogen in animal manure presents an equally important public health risk. In this study, we report the finding of two A. baumannii isolates in swine manure from a Croatian pig farm. Both isolates shared features with the widespread human clinical isolates: affiliation to the international clonal lineage 2 (ST-195), carbapenem, and extensive drug resistance and the plasmid-located acquired blaOXA-23 gene. These two A. baumannii isolates survived anaerobic conditions, competition with other microorganisms, and elevated concentrations of heavy metals in the stagnant swine manure for at least 2 weeks. These findings call for bacteriological analysis and disinfection of liquid swine manure before its application as a fertilizer in traditional extensive agriculture.

Presence of carbapenem-resistant bacteria in soils affected by illegal waste dumps.

The carbapenem-resistant bacteria (CRB) are currently at the top of the WHO priority list of bacteria that pose the greatest threat to human health. Considering that soil is one of the important environments for the emergence of antibiotic-resistant bacteria, we isolated and quantified cultivable CRB in soils across Croatia, including ones affected by illegal dumps.We cultivated CRB at two temperatures, distinguishing between the intrinsically resistant CRB (37°C, mostly Stenotrophomonas spp.) and the ones that are presumably human-associated and clinically relevant (42°C, Acinetobacter sp., Enterobacteriaceae, Burkholderia spp.). Our study demonstrated that distinguishing between the two offers a better insight into the diversity of CRB in the environment. The ones cultivated at 37°C were found in almost all soil samples, while the presumably clinically relevant ones were absent from uncontaminated pasture and grassland, indicating that human-associated CRB are unlikely to be found in soils spared from anthropogenic influence.

Mercury in Eisenia fetida and soil in the vicinity of a natural gas treatment plant in northern Croatia.

In the last two decades (1990-2012), as part of a mercury monitoring programme, earthworms and soils have been collected from four locations in the vicinity of a natural gas production and treatment plant near the village of Molve, Croatia. The aim of this study was to determine the concentration of mercury in the collected samples, monitor its changes over a longer period of time and determine the bioaccumulation of total mercury in earthworms (Eisenia fetida) from the soil. Total mercury concentrations in earthworms from the surroundings of four boreholes (Molve 9-12) ranged within 0.195-1.050, 0.129-1.0, 0.229-1.236 and 0.223-0.799 µg g-1 dry weight, while total mercury concentrations in different soil types at the same locations within 0.055-0.350, 0.035-0.250, 0.031-0.240 and 0.071-0.475 µg Hg g-1 of soil. The calculated mercury bioaccumulation factor ranged between 0.9 and 17.5. Mercury levels in soil and earthworms, as a tool for soil pollution assessment, suggested low mercury exposure and risks for human health in the monitored area.

Sulphur cycling between terrestrial agroecosystem and atmosphere.

Central gas station of the natural gas borehole system Podravina is located near the village Molve. It delivers more than a quarter of total energy used in Croatia to its consumers. Over the years, adapting technology to increasingly demanding and rigorous standards in environmental protection has become paramount. Yet, despite all the industry has undertaken to address the risk of harmful substances entering the food chain, a multidisciplinary research team of independent scientists monitors the content of specific substances in all components of the ecosystem. This paper presents measurements of total sulphur contents in soil surface [(0 to 3) cm] and subsurface [(3 to 8) cm] layers (study period: autumn 2006 - spring 2010) and in plants (study period: spring 2000 - spring 2010), and the concentration of gaseous sulphur compounds in the air. Concentrations of hydrogen sulphide (H2S) and mercaptans (RSH) were measured from the summer of 2002 until the autumn of 2010, while concentrations of sulphur dioxide (SO2) were measured from the spring of 2008 until the autumn of 2010. The paper also shows total annual atmospheric sulphur (S-SO4) deposition at Bilogora measuring station (study period: 2001 - 2010). Average monthly concentrations of H2S in air varied between 0.2 µg m-3 and 2.0 µg m-3, RSH between 0.1 µg m-3 and 24.5 µg m-3, and SO2 between 0.4 µg m-3 and 2.8 µg m-3 depending on the location and the season of sampling. Mean values of total sulphur in soil and in Plantago lanceolata plant ranged between 610 mg kg-1 and 1,599 mg kg-1 and between 3,614 mg kg-1 and 4,342 mg kg-1, respectively, depending on the soil type, location, and sampling depth. Average values of total sulphur mass ratio for all examined single soil samples (n=80) were 1,080 mg kg-1 for both studied layers, and 4,108 mg kg-1 for all analysed plant samples (n=85). Average total annual atmospheric sulphur deposition at Bilogora measuring station was 6.3 kg of S-SO4 per hectare.

Arsenic in air and soil in the vicinity of the central gas station Molve, Croatia.

Spatial and temporal distribution of arsenic levels in soil during the 9 year monitoring period was investigated on four different soil types in the area of the gas borehole system Podravina in Croatia. Arsenic levels in the PM(10) particle fraction were measured periodically at the same locations for 3 years. Arsenic levels in soil significantly depended on soil types. Elevated levels were found on gleysol vertic, at two sampling sites, with values exceeding 30 mg/kg of arsenic in soil. Arsenic levels in air were low and they were not significantly different between sampling sites, suggesting that gas borehole activities have no influence on arsenic levels in the environment.