RESUMO
The effect of repetitive pulsed X-ray (4 ns pulse duration, 300 kV accelerating voltage; 2.5 kA electron beam current) on the antioxidant enzyme activity in mouse liver mitochondria has been investigated. The mitochondrial suspension was exposed to single 4000 pulse X-ray radiation with repetition rates ranging between 10 and 22 pps (pulsed dose was 0.3-1.8 x 10(-6) Gy/pulse, the total absorbed dose following a single exposure was 7.2 x 10(-3) Gy). It was shown that a short-time exposure to X-ray radiation changes the antioxidant enzyme activity in mouse liver mitochondria. The greatest effect was observed in the changes of the activity of the metal-containing enzymes: superoxide dismutase and glutathione peroxidase. The effect depends on the pulse repetition frequency and radiation dose.
Assuntos
Antioxidantes/metabolismo , Glutationa Peroxidase/metabolismo , Mitocôndrias Hepáticas/efeitos da radiação , Superóxido Dismutase/metabolismo , Animais , Catalase/metabolismo , Catalase/efeitos da radiação , Glutationa Peroxidase/efeitos da radiação , Camundongos , Mitocôndrias Hepáticas/enzimologia , Doses de Radiação , Superóxido Dismutase/efeitos da radiação , Raios XRESUMO
The effect of 13 Hz repetition rate X-ray pulses with 3 x 10(-6)-1.5 x 10(-4) Gr per pulse dose during 5 minute on drosophila's larvae and on pupae vas investigated. It was shown that the effect depends on drosophila's age as well as on X-ray dose and manifests itself in variation of life expectancy and fertility.
Assuntos
Drosophila melanogaster/crescimento & desenvolvimento , Drosophila melanogaster/efeitos da radiação , Animais , Relação Dose-Resposta à Radiação , Fertilidade/efeitos da radiação , Larva/crescimento & desenvolvimento , Larva/efeitos da radiação , Expectativa de Vida , Raios XRESUMO
Combined effect of 460-MHz microwave irradiation and increased (up to 40 degrees C) temperature on Drosophila embryos of definite age was studied. It was demonstrated that the effect of 5-min exposures to non-modulated microwaves with 6 W/kg SAR accompanied with heating is only a little stronger than at normal temperature (24.5 degrees C). Irradiation with pulse-modulated microwaves with pulse repetition rates of 6, 10, 16, and 22 p.p.s. with average SAR of 0.12 W/kg (pulsed SAR 3 W/kg) combined with increased temperature caused some changes in PID dependent on the pulse rate. At 6 and 22 p.p.s, the increase in PID was close to that observed at normal temperature while at 10 and 16 p.p.s. the microwave irradiation did not produce any noticeable effect on development of the Drosophilas.
Assuntos
Drosophila/efeitos da radiação , Campos Eletromagnéticos , Embrião não Mamífero/efeitos da radiação , Fatores Etários , Animais , Drosophila/embriologia , Micro-Ondas , Doses de Radiação , Temperatura , Fatores de TempoRESUMO
Effect of electromagnetic radiation 460 MHz with 2.5-40 Hz pulse modulation rate on Drosophila embryos of 15 h 10 m age was studied. It was demonstrated that a 5-min irradiation with 0.12 W/kg average SAR (3 W/kg pulsed SAR) alters the Drosophila percentage of interrupted development. The effect strength depended on the modulation rate with a pronounced decrease at 10 and 16 Hz. A hypothesis about the presence of thermal and non-thermal mechanisms of action of pulse-modulated microwave radiation diversely effecting the embryos has been put forward and grounded.