A study exploring the autism awareness of first grade nursing and medical students in Istanbul, Turkey.

OBJECTIVE: To assess the level of awareness about childhood autism among first-grade nursing and medical students. METHODS: The descriptive study was conducted at Marmara University, Istanbul, Turkey, in December 2012, and comprised first-grade nursing and medical students. Data was collected using a self-administered questionnaire. Association between categorical variables was determined and p<0.05 was considered statistically significant. RESULTS: Of the 175 students, 138(78.9%) were aware of autism, 14(8%) of them being highly aware and 124(70.9%) moderately aware, whereas 37(21.1%) were not aware. There was a significant difference in the awareness level as far as gender was concerned as 102(82.9%) females and 36(69.2%) males were aware (p=0.043). Moreover, 104(59.4%) participants were aware that autism was a neurodevelopmental disorder, 62(67.4%) of them being nursing and 42(50.6%) being medical students (p<0.05). CONCLUSIONS: First-grade medical and nursing students could be considered relatively well aware of autism as their awareness level was in between that of the general public and healthcare professionals.

Chronic fluoxetine treatment changes S100B expression during postnatal rat brain development.

BACKGROUND: Fluoxetine, a selective serotonin reuptake inhibitor, is approved for treatment of childhood depression. In rats, fluoxetine influences neuronal development, but it is unclear whether it also influences glia development. S100B is a glia-derived calcium-binding protein, which may influence the development of serotonergic fibers and, vice versa, serotonin may influence the expression of S100B. OBJECTIVES: The purpose of this study was to investigate whether fluoxetine treatment influences the expression of S100B during postnatal development, and whether potential changes are regionally dependent upon the time frame of drug administration. METHODS: S100B gene expression and S100B protein expression in three different brain regions (frontal cortex, hippocampus, and striatum) were studied by real-time polymerase chain reaction (PCR) and immunohistochemistry, respectively. First, a short-term effect, 24 hours after a 14 day fluoxetine treatment (5 mg/kg/bw s.c.) of rats either from postnatal day (PD) 1 to 15, 21 to 35, or 50 to 64, was investigated. Then, the same treatment was used to analyze S100B gene and protein levels at PD 90 (long-term effect). RESULTS: At PD 90, a significant increase of gene and protein expression was observed in all regions if rats were treated during PDs 21-35, whereas treatment during other periods had no long-term effects. A short-term effect 24 hours after fluoxetine treatment was found for almost all development stages and regions, demonstrated by a significant increase of S100B. CONCLUSIONS: These results support recent research indicating a highly drug-sensitive period (i.e., periadolescence) of rat brain development. Therefore, further clinical studies should be performed to clarify whether such a sensitive period also exists in children.