Lack of association between the methylenetetrahydropholate reductase gene A1298C polymorphism and neural tube defects in a Turkish study group.

The etiology underlying neural tube defects (NTDs) is not fully understood and is believed to involve a complex milieu of genetic and environmental factors. The A1298C polymorphism in the methylenetetrahydropholate reductase gene (MTHFR) has been associated with mild risk for NTDs. In this study, the genotype distribution of the MTHFR gene A1298C polymorphism and the levels of serum homocysteine, vitamin B12, and folate were evaluated in 33 children with NTDs, their mothers, and 46 healthy controls. Genotyping of the A1298C polymorphism was performed by real-time polymerase chain reaction. The A and C allele frequencies in children with NTDs and their mothers were similar to controls (P = 0.160). The 1298AA and 1298CC genotype frequencies (P = 0.551 and 0.062, respectively) in children with NTDs and their mothers did not differ from controls. On the other hand, the 1298AC genotype frequencies in children with NTDs and their mothers were significantly different from controls (P = 0.025). The genotype frequency of 1298AC was lower in children with NTDs than in controls. There was no significant association between clinical distribution of NTDs and 1298AA/AC/CC genotypes (P > 0.05). Serum vitamin B12 levels were higher in children with NTDs than their mothers and controls (P = 0.001). There were no differences among serum homocysteine and folate levels in all groups (P = 0.494 and 0.141, respectively). Both genetic and nutritional factors are important in the etiology of NTDs. Thus, the A1298C polymorphism cannot be regarded as a major risk factor for NTDs.

Land use change analysis of Beykoz-Istanbul by means of satellite images and GIS.

Management and planning of the natural environment requires spatially accurate and timely information on land use patterns. With repetitive satellite coverage, the rapid evolution of computer technology and the integration of satellite and spatial data, the development of land use applications have become ubiquitous. The integration of Remote Sensing (RS) and Geographic Information Systems (GIS) has been widely applied and recognized as a powerful and effective tool in detecting land use change in urban areas. This paper presents the land use change analysis of the Beykoz region, which is the second largest administrative district of Istanbul. Land use changes and their impacts are monitored using Landsat (MSS - TM) and Spot 5 satellite data in the period of 1975-2001. The independent classification of each satellite image was used as a change analysis method and the resulting images were analyzed with GIS techniques. The results showed that forest area of Beykoz decreased from 80.55% to 70.5% between 1975 and 1984 and during the 1984-2001 periods, the forested area decreased from 70.5% to 68.86% and the urban growth rate was 4.65%.