Urinary CXCL9 and CXCL10 Levels and Acute Renal Graft Rejection.

BACKGROUND: Monitoring of chemokines, CXCL9 and CXCL10, in serum may present a non-invasive detection method for rejection. OBJECTIVE: To investigate the relationship between urinary levels of CXCL9 and CXCL10 and graft function following renal transplantation. METHODS: 75 living-related donor renal transplant recipients were studied. Urinary levels of chemokines were collected pre-operatively, on post-operative 1st day, 7th day, 1st month, 3rd month, and at the time of rejection. Chemokines levels were assayed using and enzyme-linked immunosorbent assay. RESULTS: Clinical variables were monitored. 10 (15%) patients had biopsy-proven rejection during the follow-up period. The urinary CXCL9 level in those with rejection was significantly higher than that in those with non-rejection group at the 1st day (p<0.001), 7th day (p<0.001), and at the time of rejection (p=0.002). The urinary CXCL10 level was also significantly higher in those with rejection compared with non-rejection group at 1st day (p<0.001), 7th day (p<0.001), and at the time of rejection (p=0.001). Serum creatinine level was strongly correlated with the urinary CXCL9 and CXCL10 levels at the time of rejection (r=0.615, p=0.002; and r=0.519, p=0.022, respectively). Among those with T cell-mediated rejections the mean urinary CXCL10 level increased to as high as 258.12 ng/mL. CONCLUSION: Urinary CXCL9 and CXCL10 levels might have a predictive value for T cell-mediated rejection in early post-transplantation period. Measurement of urinary CXCL9 and CXCL10 levels could provide an additional tool for the diagnosis of rejection.

Predictive Value of Interleukin 2 and Interleukin 8 on Early Rejection in Living Related Kidney Transplant Recipients.

INTRODUCTION: Early diagnosis of rejection in kidney transplant (KTx) recipients is of paramount importance for long-term graft survival. Cytokines play an important role in rejection via activating T cells. Neutrophil accumulation in the graft indicates cell-mediated rejection. Cellular infiltration is mediated through chemoattractant factors. The aim of this study was to investigate the relationship between graft function and serum levels of interleukin 2 (IL-2) and interleukin 8 (IL-8) in KTx. METHOD: Sixty-five patients undergoing KTx were enrolled in the study. Serum samples of IL-2 and IL-8 were collected the day before the operation, on postoperative days 1 and 7 day, and during the first and third month after the onset of rejection. The enzyme-linked immunosorbent assay method was used to determine the IL-2 and IL-8 values. RESULTS: A total of 9 (13.8%) patients had rejection documented on biopsy samples. Fifty-six patients had stable graft function (SGF). IL-2 and IL-8 values before KTx of both the rejected and SGF patients were not statistically different. Univariate analysis revealed that IL-2 and IL-8 were correlated with rejection (P = .046, P = .015). IL-8 levels were higher in the rejection group compared to the SGF group on the seventh day and first month postoperatively (P = .023, P = .038). The rejection group maintained higher levels of IL-8 for 11 days (range: 7-30) compared to the SGF group (P = .002) and the IL-8 levels correlated with serum creatinine levels (r = 0.621, P = .001). IL-2 levels were higher in the rejection group on days 1 and 7 compared to the SGF group (P = .042, P = .031). IL-2 and IL-8 levels were correlated with low eGFR in the third month in the rejection group (r = 0.421, P = .037; r = 0.518, P = .008). CONCLUSION: Determining the cytokine levels in the early post-KTx period may be helpful in tailoring immunosuppressive regimens in patients with a risk of rejection.

[Large Hibernoma of the Proximal Upper Arm and the Axilla - Literature Review and Case Description of a Very Rare Benign Soft Tissue Tumor]. / Großes Hibernom des proximalen Oberarms und der Axilla ­ Literaturüberblick und Fallbeschreibung eines sehr seltenen gutartigen Weichteiltumors.

Hibernomas are very rare benign soft tissue tumors arising from brown fat. Malignant transformation or metastases are unknown. Males seems to be affected more often. Most patients are aged 20 to 40, but patients with intraosseous hibernomas are older. In children, hibernomas are extremely rare. The tumors grow slowly and have usually been present for a few years on presentation. Hibernomas are typically located on the thigh, neck, axilla or in the peri- und interscapular region. Diagnostic work-up in symptomatic hibernomas usually includes conventional X-ray and magnetic resonance tomography (MRI) with contrast medium. Asymptomatic hibernomas are often found accidentally in the diagnostic work-up of other diseases. Important differential diagnoses are lipomas, well differentiated liposarcomas, rhabdomyomas, granular cell tumors and sebeceous adenomas. Incisional biopsy should be performed to allow definitive histological diagnosis before definitive therapy. According to the literature, histologically preserved hibernomas can be removed with curative intention and marginal resection. After complete tumor removal, local recurrence has not been described. The following article describes the case of a large hibernoma of the proximal arm, involving the axilla, and describes the epidemiology, clinical behavior, diagnostic work-up, therapy and prognosis of this very rare benign fatty soft tissue tumor, on the basis of a review of current literature.

Effect of Donor Nephrectomy Technique on Recipient Lymphatic Drainage.

AIM: We sought to evaluate the postoperative recipient lymphatic drainage depending on open donor nephrectomy (ODN) or laparoscopic (LDN) techniques. METHOD: Between March 2012 and August 2014, 58 patients underwent renal transplantation from living-related donors. Thirty donors underwent ODN (group 1), and 28 LDN (group 2). Operations were performed by the same surgeons. Both cranial and caudal drainage catheters for lymphatic leakage were placed preoperatively and all the recipients received tacrolimus, mycophenolate mofetil, and steroid as immunosuppressive regimen. None of the patients had coagulation abnormalities. RESULTS: All grafts were functioning during the early postoperative period and diuresis was ensured. No difference was observed on early postoperative period regarding to acute rejection (P = .329) or infection (P = .546). No difference was seen concerning mycophenolate mofetil and mycophenolate sodium regimens among the 2 groups (P = .227). In groups 1 and 2, the cranial drainage catheters were not taken out until postoperative days 5.5 ± 2.5 (range, 0-11) and 6.4 ± 3.8 (range, 0-14) and the caudal catheters stayed in place until days 8.8 ± 3.5 (range, 1-16) and 9.9 ± 5.9 (range, 3-22), respectively. No difference was found when comparing the cranial (P = .308) and caudal (P = .426) drainage periods. However, during clinical acute rejection episodes the cranial drainage period was longer in group 1 (P = .003). Three patients developed lymphoceles, 1 requiring drainage, in group 2. CONCLUSIONS: There seems to be no difference in recipient lymphatic drainage by donor nephrectomy technique. A laparoscopic procedure may be advantageous owing to shorter lymphatic drainage during clinical acute rejection episodes.

Alternative technique of cement augmentation of loosened pedicle screws -- technical note and presentation of two cases.

An alternative cement augmentation technique for pedicle screws is described, which was applied in two patients with mono- and bisegmental non-union after preceding multisegmental lumbar fusion. The correctly placed pedicle screws in S1 with diameters of 6 and 8.5 mm had severely enlarged the screw cavities due to segmental instability. Revision screws with 10 mm diameter demonstrated sufficient purchase only on the left side. Therefore, cement augmentation was performed for the right sided screws. After verification of intact pedicle borders, the cavity was filled up with PMMA bone cement. Afterwards, a Kirschner wire was positioned centrally, the hardening of the cement was awaited, the cement was gradually drilled and the screw was placed. In both patients, sufficient purchase of the cemented pedicle screws was documented. Screw insertion after awaiting the hardening of the bone cement in pedicles and vertebral bodies with huge defect situations seems to be an alternative to previous cement augmentation techniques of pedicle screws with the advantage, that the screws could be more easily unscrewed, if necessary.

[Adjacent segment movement after monosegmental total disc replacement and monosegmental fusion of segments L4/5]. / Anschlusssegmentbeweglichkeit nach monosegmentaler Bandscheibenprothesenimplantation und monosegmentaler Fusion Segment L4/5.

BACKGROUND: Preservation of movement at the treated segment and possible reduction of adjacent segment effects is assumed to be an advantage of non-fusion technologies over fusion. The aim of this study was to compare the segmental range of motion (ROM) at the operative level, the cranial and caudal adjacent levels and the global lumbar spine ROM (L2-S1) after monosegmental fusion and total disc replacement (TDR). PATIENTS AND METHODS: Radiographic data was collected from 27 patients with level 1 degenerative disc disease operated at level L4/5. The ROM was assessed at the index level (L4/5), the cranial and caudal adjacent level and for the lumbar spine (L2-S1). RESULTS: In the TDR group no significant changes of lumbar spine ROM (L2-S1) and segmental ROM (index level, cranial and caudal adjacent level) were noticed. In the fusion group there was a significant reduction of lumbar ROM (L2-S1) and index level ROM. Additionally the relative ROM in the adjacent caudal segment significantly increased while no changes were seen in the cranial segment. CONCLUSION: The relative ROM was significantly increased in monosegmental fusion at level L4/5 compared to TDR. To what extent this fact may result in early adjacent segment degeneration in cases of fusion compared to TDR is still unknown.

[Clinical results of surgically treated paediatric fractures of the medial humeral epicondyle]. / Klinische Ergebnisse bei operativ versorgten kindlichen Frakturen des Epicondylus humeri ulnaris.

OBJECTIVES: Fractures of the medial humeral epicondyle represent approximately 10 % of all paediatric elbow fractures. material and methods: Surgically treated paediatric fractures of the medial humeral epicondyle were analysed retrospectively for their epidemiological, clinical and surgical parameters. Re-evaluation included clinical function, satisfaction, pain level and MAYO elbow performance score (MEPS). RESULTS: 22 children could be included. A re-evaluation of 91 % of our patients after a follow-up of ∅ 4 years (range: 1-9 years; median: 4 years) after initial surgery was possible. Subjective outcome and objective function were good with moderate limitations. 85 % of our patients showed good to excellent results in the MEPS. CONCLUSION: Regarding our own good clinical results and potentially lower rates of pseudarthrosis - in comparison to conservatively treated patients - by trend we recommend internal fixation in paediatric fractures of the medial humeral epicondyle. However, operative or conservative treatment must be indicated individually together with the patient and his/her parents.

[Results of surgically treated paediatric supracondylar humeral fractures]. / Behandlungsergebnisse operativ versorgter kindlicher suprakondylärer Humerusfrakturen.

BACKGROUND: About 80 % of all paediatric distal humerus fractures are supracondylar fractures. PATIENTS AND METHODS: Surgically treated children with supracondylar humeral fractures between 2000-2008 were analysed retrospectively and re-evaluated for function, satisfaction, pain level and with the MAYO elbow performance score (MEPS). RESULTS: 46 patients were included. Open fractures (2 %) and vessel (0 %) or nerve (4 %) lacerations were rare, additional forearm fractures frequent (15 %). Surgery was done by closed/open reduction and crossed K-wire pinning. Main complications were movement restriction and K-wire migration. All fractures healed. 72 % of patients could be re-evaluated Ø 51 months after surgery. The mean differences between non-affected and affected elbows showed 8° for flexion, 1° for extension, 1° for pronation and 0° for supination. In 88 % excellent or good results could be measured with the MEPS. CONCLUSION: Regarding bony healing in all patients, well manageable complications, mostly excellent or good results in the MEPS and good function, crossed K-wire pinning after closed/open reduction is a safe standard surgical procedure for this type of fracture.

Is heparinization necessary in the early postoperative period of renal transplantation from cadaveric donors?

INTRODUCTION: Venous thromboemboli and bleeding are the complications that threaten the graft and patient's life in the early postoperative period after cadaveric renal transplantation. For this reason, heparin administration after renal transplantation should be administered carefully. The aim of this study was to evaluate the necessity for heparinization after cadaveric renal transplantation. METHODS: Between March 2009 and October 2010, we formed 2 study groups among 50 recipients who underwent either cadaveric (n = 25) or living donor transplantations (n = 25). We did not observe any risk factors for thromboembolism while group 1 did not undergo heparinization, group 2 received a prophylactic dose of low-molecular weight heparin for 1 week. Doppler ultrasonography (USG) was performed between postoperative 24-48 hours to examine the transplanted kidney vessels, and in one group 1 case for a bilateral lower extremity venous system examination. We were also compared postoperative thromboembolic and hemorrhagic complications, lymphorrhagia, and serum creatinine levels. RESULTS: The female/male ratios in group 1 and 2 were 14/11 and 8/17 with mean ages of 36.7 (range, 17-51) and 35.9 (range, 17-59) years, respectively. The mean preoperative serum creatinine levels were 7.9 ± 2.9 mg/dL and 6.8 ± 2.4 mg/dL, and at postoperative week 1, they were 5.1 ± 4.3 mg/dL and 1.2 ± 0.5 mg/dL, respectively. We did not encounter any partial or total thrombus upon doppler USG studies for renal and lower extremity venous systems. No clinical symptoms of pulmonary emboli were detected in any patients. Only 1 subject group 2 experienced massive postoperative bleeding. CONCLUSION: Herein, we have reported that, except for the patients with risk factors for venous thromboemboli, heparinization was not necessary in the early postoperative period and did not add benefits to outcomes of cadaveric renal transplant recipients.

[Non-union of pediatric lateral humeral condyle following post-traumatic cubitus valgus]. / Pseudarthrose des kindlichen Condylus radialis humeri mit posttraumatischem Cubitus valgus.

Non-union of the pediatric lateral humeral condyle following post-traumatic cubitus valgus is a severe complication after inadequate treatment. We report on a then 14-year-old male patient who developed an increasingly defective position in terms of cubitus valgus after conservative treatment of a lateral humeral condyle fracture. After performing screw osteosynthesis and achieving consolidation, a successful supracondylar dome osteotomy was performed which led to relief from pain and free range of motion of the elbow joint.

[Perioperative morbidity in lumbar disc replacement]. / Perioperative Morbidität bei lumbaler Bandscheibenprothesenimplantation.

AIM: For several years now interbody fusion has been the gold standard procedure for treating degenerative disc disease. The problem of adjacent disc degeneration after interbody arthrodesis led to the development of non-fusion techniques. The device which best represents the philosophy of spine arthroplasty is the total lumbar disc replacement (TDR). An analysis of the perioperative morbidity of lumbar disc replacement was carried out in the current study. METHOD: 66 patients underwent lumbar disc replacement between 2001 and 2007. 78 protheses were implanted. Retrospectively patient-related variables (comorbidity, prior surgeries), perioperative blood loss, number and levels operated on, operation duration and technical and general complications were analysed. RESULTS: 54 patients had mono- and 12 patients bisegmental TDR. The mean operation time was 112 minutes with an average blood loss of 560 mL. Neither the type of comorbidity, prior surgery, operation duration, nor level operated on had an influence on the occurrence of perioperative morbidity. A significant influence could be shown for the number of levels operated on and the intraoperative blood loss. General complications were seen in 6 persons (9%) with urinary tract infection, technical complications occurred in 4 persons (6%) with severe blood loss (> 1500 mL) and erythrocyte/plasma substitution, 1 person (1.5%) with an injury of the iliac vein, 1 person (1.5%) with retrograde ejaculation and 1 person (1.5%) with dural tear and consecutive epidural infection. CONCLUSION: In our study the perioperative morbidity of lumbar disc replacement was similar to the data published so far and seems to be comparable with the perioperative morbidity of lumbar interbody fusion.

Screw loosening after posterior dynamic stabilization--review of the literature.

PURPOSE OF THE STUDY: The malposition of pedicle screws in the lumbar spine is associated with a potential risk of iatrogenic injury of neurological structures but also with a decrease of biomechanical stability. The correct position of the pedicle screws of a dynamic stabilization device, as a long-term implant, seems to be of great importance. A high incidence of screw loosening could influence both the rate of revision surgeries and the clinical results.We compared screw loosening in our own patients with published data after navigated and non-navigated implantation of Dynesys. MATERIAL AND METHODS: Posterior instrumentation with the Dynesys system was performed in 19 patients after improvement of facet joint infiltrations. Seven (37%) patients underwent conventional surgery (group I), five (26%) were operated on using a CT-based navigation (group II) and seven (37%) using a fluoroscopic-based navigation (group III). Pre-operatively, the "Oswestry Low Back Pain Disability Questionnaire (OQ)" and the "Short Form 36 Health Survey Questionnaire (SF-36)" were used to obtain pain and functional scores. Furthermore, radiographs, MRI and CT examinations were performed before surgery. Screw position was analyzed on post-operative CT scans. At a minimum follow-up of twelve months, clinical examination, plain and dynamic X-rays were performed, and pain and functional scores (OQ, SF-36) were obtained. RESULTS: Pedicle perforation of minimum 2 mm was detected in two group I patients, in one group II and in two group III patients. Regarding OQ and SF-36, an improvement was observed in all patients, except for one column of SF-36. One patient (group I) underwent revision surgery due to symptomatic screw loosening and another patient (group III) due to persistent pain without signs of screw loosening. DISCUSSION: In former publications the majority of patients improved after Dynesys implantation with or without the use of navigation methods.Malposition of screws was not always followed by screw loosening. Revision surgery due to screw loosening, but without clinical symptoms, was not necessary in the majority of cases. CONCLUSIONS: It still remains unclear if screw loosening after Dynesys implantation influences the clinical results or the rate of revision surgery and if malposition of screws will be followed by a higher rate of screw loosening.

[Perioperative morbidity of lumbar stabilisation with Dynesys]. / Perioperative Morbidität bei Dynesys-Instrumentierung der Lendenwirbelsäule.

AIM: Several non-fusion technologies have been developed as an alternative to fusion procedures in the past decades, in order to avoid typical problems related to fusion. The Dynesys (Zimmer, USA), which was inaugurated 7 years ago, consists of pedicle screws, flexible spacers and cords and is intended to control segmental motion and to realign the lumbar spine. Since this device is new, it was the goal of this study to analyse the perioperative morbidity of lumbar stabilisation with Dynesys retrospectively. METHOD: 136 patients (74 men and 62 women) underwent posterior stabilisation of the lumbar spine with Dynesys between January 2002 and April 2006. We acquired retrospectively several patient-related variables, e.g. the number of instrumented and decompressed segments, the perioperative blood loss, the operation duration, prior surgeries and technical and general complications. RESULTS: The average age of the patients was 61.2 years (range: 33-84). 1.6 segments (range: 1-3) were instrumented and 1.2 segments (range: 0-3) were decompressed on average. The mean perioperative blood loss was 1100 ml (range: 50-3400) and the mean operation duration was 127.1 minutes (range: 30-270). Eighteen patients (13.2 %) had a urinary tract infection and 5 patients (3.7 %) had cardiovascular complications. Dura lesions were seen in 11 patients (8.1 %) with 1 patient having persistent bladder symptoms. Revision surgery was necessary in 3 patients (2.2 %) due to screw malposition with neurological deficits and radicular pain. Five patients (3.7 %) were revised due to postoperative seroma, 2 of them developing superficial infection. CONCLUSION: The perioperative morbidity of lumbar stabilisation with Dynesys seems to be similar to posterior fusion techniques. However, an additional morbidity due to interbody fusion, which may necessitate also autogenous bone, is avoided.

[Embolisation of aneurysmal bone cysts as a minimally invasive treatment option - presentation of two cases]. / Die Embolisation stammnaher aneurysmatischer Knochenzysten als minimalinvasive Therapieoption - zwei Fallbeispiele.

AIM: The aim of this study was to evaluate embolisation as a therapy option for aneurysmal bone cysts of the trunk. METHOD: Case reports about two males with intermittent pseudo-radicular lumboischialgia and coxalgia are discussed. RESULTS: The diagnostic work-up and biopsies verified an aneurysmal bone cyst in both males. In one patient the tumour-like lesion was localised in the fifth lumbar vertebral body, in the other in the left ischium and pubis. Arterial embolisation was performed in both cases. Follow-up at 6 and 24 months after embolisation showed a significant increase of sclerosis and a reduced volume of the cysts. CONCLUSION: In accord with literature data, arterial embolisation seems to be a sufficient and minimally invasive therapy option in aneurysmal bone cysts of the spine and the pubis.

[The prognostic influence of primary tumour and region of the affected spinal segment in 217 surgical patients with spinal metastases of different entities]. / Der prognostische Einfluss von Primärtumor und Höhe des befallenen Wirbelsäulenabschnitts bei 217 operativen Patienten mit Wirbelsäulenmetastasen unterschiedlicher Entität.

AIM: A retrospective study to evaluate the prognostic influence of the primary tumour and the anatomic level of spinal metastases was carried out. MATERIAL AND METHODS: Between January 1984 and May 2005, 217 patients were surgically treated because of spinal metastases. The prognostic influence for the survival was analysed for the entity of the primary tumour and the localisation of the spinal metastases. RESULTS: The median survival of the study group was 8.0 months (range: 0-191.5 months). Mamma carcinoma was the most frequent primary tumour with 62 cases (28.6 %). The spinal level of the metastases did not influence the postoperative survival (p = 0.9058). The entity of the primary tumour showed a significant influence for the postoperative survival (p < 0.0001). CONCLUSION: In spinal metastases, the entity of the primary tumour was of prognostic value; the localisation of the spinal metastases at different spinal levels did not influence the postoperative survival. Therefore, the evaluation of the primary tumour is mandatory for an estimation of the expected survival.

Cystatin-C and creatinine as indices of glomerular filtration rate in the immediate follow-up of renal transplant patients.

Serum cystatin-C (cys-C), creatinine (Cr), C-reactive protein (CRP) and amyloid A have been shown to provide useful information for renal function following transplantation. In this study, we wanted to evaluate the impact of these parameters as markers of the glomerular filtration rate (GFR) on the third and seventh days of the post-transplantation period. Cys-C was determined by the particle-enhanced immunoturbidimetric assay, and serum amyloid A (SAA) by the sandwich-enzyme immunoassay kit. Cr and CRP concentrations were measured by the Cobas Integra 400 autoanalyser. The patients (n=35) were followed with daily repetitive measurements of serum Cr and urine output per hour, and with Doppler ultrasonography against the risk of rejection. Statistical evaluations were made using the ANOVA and Pearson's test. Serum cys-C and Cr levels on both the 3rd and 7th days after transplantation were lower than those of pretransplantation values (P<0.001). The Cr/cys-C ratio was decreased on the 3rd day of the post-transplantation period, and kept declining on the 7th day. This ratio was high only in the patient with an acute rejection episode. None of the patients with high pretransplant CRP levels had a rejection episode during a six-month follow-up. SAA concentrations were found to be higher than the pretransplant values in the early post-transplant period. Cys-C had good sensitivity to estimate the renal function in the very early period of transplantation, but its value as a marker of GFR was decreased at the end of first week. As none of the 34 patients had a rejection episode, the observed rise in SAA and CRP levels is not specific to rejection.

[The effects of application of rectal naproxen on postoperative analgesia, sedation and morphine use in heart surgery operations]. / Kalp cerrahisinde rektal naproksenin postoperatif analjezi, sedasyon ve morfin kullanimi üzerine etkileri.

In this study, effects and side effects of application of rectal naproxen, combined with patient controlled intravenous morphine analgesia, were investigated in the elective coronary bypass operations for postoperative pain control, sedation and opioid use. Following the ethical committee approval and individual patient self consent, 40 patients, who underwent coronary artery bypass surgery were included in the study. A double blind study was performed by administering rectal naproxen to group N (n = 20) and placebo to group P (n = 20), at the end of the operation. Doses were repeated at the 12th hour postoperatively. Patient controlled intravenous morphine analgesia was performed to all patients for postoperative 24 hours. Postoperative pain and sedation levels were assessed, the side effects were noted. There was no difference between two groups with respect to their demographic features duration of surgery, extubation time and side effects (p > 0.05). With respect to group P, decrease in opioid use, better sedation and decrease in pain scores during both resting and coughing was seen in group N (p < 0.05). In conclusion, analgesia applied by addition of rectal naproxen to opioids achieved better pain management in selected patients after cardiac surgery.

Design and implementation of a random neural network routing engine.

Random neural network (RNN) is an analytically tractable spiked neural network model that has been implemented in software for a wide range of applications for over a decade. This paper presents the hardware implementation of the RNN model. Recently, cognitive packet networks (CPN) is proposed as an alternative packet network architecture where there is no routing table, instead the RNN based reinforcement learning is used to route packets. Particularly, we describe implementation details for the RNN based routing engine of a CPN network processor chip: the smart packet processor (SPP). The SPP is a dual port device that stores, modifies, and interprets the defining characteristics of multiple RNN models. In addition to hardware design improvements over the software implementation such as the dual access memory, output calculation step, and reduced output calculation module, this paper introduces a major modification to the reinforcement learning algorithm used in the original CPN specification such that the number of weight terms are reduced from 2n/sup 2/ to 2n. This not only yields significant memory savings, but it also simplifies the calculations for the steady state probabilities (neuron outputs in RNN). Simulations have been conducted to confirm the proper functionality for the isolated SPP design as well as for the multiple SPP's in a networked environment.

Urologic complications of extravesical ureteroneocystostomy in renal transplantation from living related donors.

INTRODUCTION: Reconstruction of the urinary system during renal transplantation is usually performed with antirefluxive ureteroneocystostomy techniques and extravesical methods are usually preferred. MATERIAL AND METHODS: Between 1983 and 1997, 241 renal transplantations from living donors were performed at our institution. A variation of the Lich-Gregoir technique was used as the ureteroneocystostomy method in all cases. RESULTS: A total of 12 (4.9%) urologic complications were observed. Urinary fistula developed in 5 (2%) cases and were explored surgically during the early postoperative period. Postoperative vesicoureteral reflux (VUR) to the transplanted kidney was identified in 7 (2.9%) cases. Either endoscopic or surgical interventions resolved VUR in 4 cases while the other 3 did not need further treatment. No ureteral stenosis was observed. There was no loss of graft due to urologic complications. CONCLUSIONS: Urologic complications after renal transplantation are reported to be about 7% and ischemia is blamed as the major contributing factor. Preparation of the native ureter during donor nephrectomy and preservation of distal periureteral fatty tissue, anastomosis technique variations in vascular anatomy, rejections and medications are the major factors determining the ischemia.

In vitro effects of peroxynitrite on human spermatozoa.

In the present study, the in vitro effects of peroxynitrite on sperm motility, lipid peroxidation and sulfhydryl content were examined. Sperm percentage motility and movement characteristics were assessed by a computer-assisted system. Lipid peroxidation was measured by determining malondialdehyde levels using the thiobarbituric acid (TBA) method. Sperm sulfhydryl content was measured by a spectrophotometric method based on reduction of 5,5'-dithiobis-(2-nitrobenzoic acid) by sulfhydryl groups. Percentage motility, movement characteristics and sulfhydryl content decreased significantly in peroxynitrite-treated samples compared to decomposed peroxynitrite-treated samples. Lipid peroxidation in peroxynitrite-treated samples was significantly higher than in decomposed peroxynitrite-treated samples. These results indicate that peroxynitrite anion may cause sperm dysfunction through lipid peroxidation stimulation and total SH depletion.