RESUMO
The article considers molecular genetic characteristic of RNA of human enterovirus detected in bio-test from child with serous meningitis. The nucleotide sequence of genome DNA is analyzed. In 98% it is identical to corresponding nucleotide sequences of strains of human enterovirus A serotype 71 detected in China.
Assuntos
Enterovirus Humano A/isolamento & purificação , Infecções por Enterovirus/virologia , RNA Viral/isolamento & purificação , Sequência de Bases , China , Diagnóstico , Enterovirus Humano A/genética , Infecções por Enterovirus/diagnóstico , Infecções por Enterovirus/genética , Infecções por Enterovirus/mortalidade , Humanos , Meningite/mortalidade , Meningite/virologia , Filogenia , RNA Viral/genética , Federação RussaRESUMO
The article presents data concerning analysis of bioassays under suspicion of influenza A(H1N1)pdm09. The technique of back transcription polymerase chain reaction and sequencing was applied. The separation of native influenza virus A was implemented using passaging through developing chick embryos.
Assuntos
Hemaglutininas Virais/genética , Vírus da Influenza A Subtipo H1N1/genética , Influenza Humana/virologia , RNA Viral/isolamento & purificação , Animais , Embrião de Galinha , Hemaglutininas Virais/isolamento & purificação , Sequenciamento de Nucleotídeos em Larga Escala , Humanos , Vírus da Influenza A Subtipo H1N1/isolamento & purificação , Vírus da Influenza A Subtipo H1N1/patogenicidade , Vírus da Influenza A Subtipo H1N1/ultraestrutura , Influenza Humana/genética , Influenza Humana/patologia , Microscopia Eletrônica , RNA Viral/genéticaRESUMO
Experiments indicated that the argasid ticks Alveonasus lahorensis were highly susceptible to West Nile virus when inoculated in the hemocoel. The virus concentration in the ticks reached high values when very low doses (0.01 PFU) of the pathogen were administered. The ticks kept at 3.0 +/- 1.0 degrees C retained the pathogen up to 116 days (a follow-up period). The infection rate of the ticks depending on the virus dose administered was in the range from 12 to 80%. The contaminated specimens successfully transmitted the virus to rabbits by blood suckling. The findings suggest that the argasid ticks may be involved in the preservation of West Nile virus in the interepidemic period and be responsible for the outbreak of this infection in summer and autumn months.