Corrigendum to ''Sampling protocol for the determination of nutrients and contaminants in fish and other seafood - The EAF-Nansen programme" [MethodsX 7 (2020) 101063].

[This corrects the article DOI: 10.1016/j.mex.2020.101063.].

Sampling protocol for the determination of nutrients and contaminants in fish and other seafood - The EAF-Nansen Programme.

Seafood plays a central role in global food and nutrition security. However, there is a lack of data on the concentration of nutrients and contaminants in fish and other seafood, especially in low- and middle-income countries. In order to assess the potential risks and benefits associated with seafood intake, reliable and up-to-date food composition data is crucial. The quality of food composition data is affected by several factors, such as sampling protocols and the suitability and quality of the methods applied for sample preparation and analysis. In this paper, we describe the sampling methodology and protocols related to the sampling of fish and other seafood and the corresponding analytical methods used to analyse the nutrient and contaminant content of such species. For nutrients, the determination of protein, fat, ash, energy, fatty acids, cholesterol, and amino acids is described, in addition to analyses for determination of the vitamin and mineral content in fish and other seafood. For contaminants, analyses for the determination of organic pollutants and microplastics are described. The methodology described in this paper is used for sampling data through scientific surveys in low- and middle-income countries with research vessel Dr. Fridtjof Nansen under the EAF-Nansen Programme. The Programme aims to improve knowledge on the nutritional composition of fish and ensure the fish is safe to consume.•In this paper, we describe the sampling protocols used for sampling fish and other seafood during scientific surveys under the EAF-Nansen Programme.•This paper describes the methodology and quality control for analysing nutrients and contaminants in fish and other seafood.

Dietary vitamin A supplementation ameliorates the effects of poly-aromatic hydrocarbons in Atlantic salmon (Salmo salar).

Several studies have reported on the interaction between vitamin A (VA) and aryl hydrocarbon receptor (AhR)-binding toxicants, including poly-aromatic hydrocarbons (PAHs). In aquaculture, the use of plant oils in novel aquafeeds can increase PAH levels while simultaneously lowering natural VA background levels, causing the need to supplement plant oil-based feeds with synthetic VA. To study dietary VA-PAH interactions, Atlantic salmon (initial weight 195±0.15g) were fed four identical plant-based diets that were supplemented with PAHs (100 and 10mgkg(-1) benzo[a]pyrene (BaP) and phenanthrene (Phe), respectively) or VA (retinyl acetate 8721IUkg(-1)) separately or combined for 2.5 months in a 2×2 factorial design, with triplicate net-pens per diet. Dietary PAH significantly reduced hepatic VA storage, and VA-enriched diets restored hepatic VA. There was a significant PAH-VA interaction effect on hepatic BaP, but not Phe, accumulation, with reduced hepatic BaP concentrations in fish fed VA+PAH compared to fish fed PAH alone. Concurrently, PAH and VA significantly interacted in their effects on CYP1A phase I biotransformation as observed from increased ethoxyresorufin-O-deethylase (EROD) activity, increased CYP1A protein concentration, and elevated transcription (cyp1a1 gene expression) in fish fed PAH+VA compared to PAH alone. Dietary VA supplementation alone had no significant effect on CYP1A phase I biotransformation. Metabolomic assessment showed that dietary VA caused a restoration of metabolic intermediates involved in energy metabolism that were affected by dietary PAH. Moreover, a PAH-induced growth inhibition was partially ameliorated by dietary VA supplementation. In conclusion, dietary VA interacted with PAH toxicity on the level of CYP1A-mediated detoxification, hepatic PAH accumulation, energy allocation, and growth.

Sensitivity of Atlantic salmon (Salmo salar) to dietary endosulfan as assessed by haematology, blood biochemistry, and growth parameters.

The presence of the organochlorine pesticide endosulfan in the water column confers a significant direct risk to the biota therein, yet relatively little is known regarding the toxic impact of dietborne endosulfan to aquatic organisms. Pre-smolt Atlantic salmon (Salmo salar) were fed at levels of technical endosulfan equal to the European Union regulatory limit (5 microg kg(-1)) and at levels 10 and 100 times greater, for a total of 49 days with haematology (e.g. erythrocyte count, haemoglobin, haematocrit, white blood cell composition), blood biochemistry (e.g. serum aminotransferase, plasma ions) and growth parameters (e.g. condition factor) recorded at days 0, 14, 35, and 49. Toxicological assessment of the individual alpha and beta-isomers that comprise technical endosulfan was also attempted. No mortality was observed in any group during the experiment. In the groups exposed to the control, 5, and 50 microg kg(-1) technical endosulfan feeds no significant alterations in any measured parameters were determined at any time point. No differences were observed between the technical mixture and the individual alpha and beta-isomer treatment groups. Condition factor was significantly reduced in fish exposed to 500 microg kg(-1) at day 49, while haematocrit, haemoglobin and mean corpuscular haemoglobin were significantly elevated after 35 days of exposure to the same dietary level, but returned to control levels by day 49. The present study shows that with regards to acute toxicity, Atlantic salmon are able to tolerate dietary technical endosulfan levels up to 500 microg kg(-1).