Dry Matter Yield Stability Analysis of Maize Genotypes Grown in Al Toxic and Optimum Controlled Environments.

Breeding for Al tolerance is the most sustainable strategy to reduce yield losses caused by Al toxicity in plants. The use of rapid, cheap and reliable testing methods and environments enables breeders to make quick selection decisions. The objectives of this study were to (i) identify high dry matter yielding and stable quality protein maize (QPM) lines grown under Al toxic and optimum conditions and (ii) compare the discriminating power of laboratory- and greenhouse-based testing environments. A total of 75 tropical QPM inbred lines were tested at seedling stage for dry matter yield and stability under optimum and Al toxic growing conditions across six laboratory- and greenhouse-based environments. The nutrient solution method was used for the laboratory trials, while the soil bioassay method was used for the greenhouse trials. A yield loss of 55% due to Al toxicity was observed, confirming the adverse effects of Al toxicity on maize productivity. The ANOVA revealed the presence of genetic variation among the set of genotypes used in this study, which can be exploited through plant breeding. Seventeen stable and high-yielding lines were identified and recommended. Greenhouse-based environments were more discriminating than laboratory environments. Therefore, we concluded that greenhouse environments are more informative than laboratory environments when testing genotypes for Al tolerance.

SNP-based assessment of genetic purity and diversity in maize hybrid breeding.

Assessment of genetic purity of parental inbred lines and their resultant F1 hybrids is an essential quality control check in maize hybrid breeding, variety release and seed production. In this study, genetic purity, parent-offspring relationship and diversity among the inbred lines were assessed using 92 single-nucleotide polymorphism (SNP) markers. A total of 188 maize genotypes, comprising of 26 inbred lines, four doubled haploid (DH) lines and 158 single-cross maize hybrids were investigated in this study using Kompetitive Allele Specific Polymerase Chain Reaction (KASP) genotyping assays. The bi-allelic data was analyzed for genetic purity and diversity parameters using GenAlex software. The SNP markers were highly polymorphic and 90% had polymorphic information content (PIC) values of > 0.3. Pairwise genetic distances among the lines ranged from 0.05 to 0.56, indicating a high level of dissimilarity among the inbred lines. A maximum genetic distance of (0.56) was observed between inbred lines CKDHL0089 and CML443 while the lowest (0.05) was between I-42 and I-40. The majority (67%) of the inbred lines studied were genetically pure with residual heterozygosity of <5%, while only 33% had heterozygosity levels of >5%. Inbred lines, which were not pure, require purification through further inbreeding. Cluster analysis partitioned the lines into three distinct genetic clusters with the potential to contribute new beneficial alleles to the maize breeding program. Out of the 68 hybrids (43%) that passed the parent-offspring test, seven hybrids namely; SCHP29, SCHP95, SCHP94, SCHP134, SCHP44, SCHP114 and SCHP126, were selected as potential candidates for further evaluation and release due to their outstanding yield performance.