RESUMO
In organotypic tissue culture, the effect of the 20 coded amino acids combinations, in the effective concentration of 0.05 ng/ml, on the cell proliferation development in the spleen, myocardium, pancreas and brain cortex explants in 24 months rats was investigated. The combinations of two amino acids (stimulating and inhibiting proliferation) produced in the tissue culture the stimulating effect, exceeding by 8-10% as compared to the isolating effect of the stimulating amino acid. The results obtained form the base for the further synthesis of new effective dipeptides to be used to increase the regeneration of tissues when aging.
Assuntos
Aminoácidos , Apoptose/efeitos dos fármacos , Proliferação de Células/efeitos dos fármacos , Fatores Etários , Aminoácidos/farmacocinética , Aminoácidos/farmacologia , Animais , Encéfalo , Linhagem Celular , Fígado , Miocárdio , Pâncreas , Ratos , Ratos Wistar , Transdução de Sinais , Técnicas de Cultura de Tecidos/métodosRESUMO
The aspects of the regulatory effect of 20 coded amino acids on the basic cellular processes--proliferation and apoptosis--are discussed. This effect is performed due to the regulation by the amino acids of the specific genes at the levels of the transcription and translation. This leads to the triggering of the regulation of any cellular processes. The investigations in organotypic culture of the tissues of the different genesis have demonstrated that the different amino acids perform the cell proliferation or apoptosis. The group of low molecular mass hydrophile amino acids with the charge chains stimulated the cell proliferation in the tissues of mesodermal genesis. The other group of high molecular mass hydrophobe amino acids stimulated the cell proliferation in the tissues of ectodermal genesis. Thus, the coded amino acids are not only the structural elements of the proteins, but can participate in the regulation of the specific genes, controlling the cellular cycle. The number of the active amino acids is decreased in 2.7 times in the explants from the old rats, as compared to the young, reflecting the disturbances in the amino acid transport and gene expression by the aging.
Assuntos
Envelhecimento/genética , Aminoácidos/metabolismo , Apoptose/genética , Proliferação de Células , Regulação da Expressão Gênica , Biossíntese de Proteínas/genética , Fatores Etários , Aminoácidos/química , Aminoácidos/genética , Animais , Código GenéticoRESUMO
The effect of 20 coded amino acids and synthetic bioregulatory tripeptides, in the effective concentration 0.05 ng/ml, was investigated in organotypic tissue culture on the cell proliferation development in the cartilage tissue explants in young 3 and old 24 months old rats. The number of the amino acid stimulated proliferation was decreased in the tissue culture from the old rats in 6 times, as compared to the young rats. The tripeptides demonstrated the stimulated proliferation effect in the tissue culture both from the young and old animals, what is a base for the further study as the geroprotective preparations.
Assuntos
Envelhecimento/efeitos dos fármacos , Envelhecimento/patologia , Aminoácidos/farmacologia , Cartilagem/efeitos dos fármacos , Oligopeptídeos/farmacologia , Animais , Apoptose/efeitos dos fármacos , Cartilagem/patologia , Proliferação de Células/efeitos dos fármacos , Masculino , Ratos , Ratos Wistar , Técnicas de Cultura de TecidosAssuntos
Aminoácidos/farmacologia , Aprendizagem por Associação/efeitos dos fármacos , Abelhas/fisiologia , Aminoácidos/metabolismo , Animais , Aprendizagem por Associação/fisiologia , Abelhas/metabolismo , Comportamento Alimentar/efeitos dos fármacos , Memória de Longo Prazo/efeitos dos fármacos , Memória de Curto Prazo/efeitos dos fármacosRESUMO
The effect of 20 coded amino acids in concentration 10(-12) M was investigated in organotypic tissue culture on the cell proliferation development in myocardium, pancreas, brain cortex tissue (the tissues of meso-, ento- and ectodermal genesis accordingly) explants in 3- and 24-months old rats. The different effect was observed in the tissues of different genesis. The group of low molecular mass hydrophile amino acids with the charge chains stimulated the cell proliferation in the young and old rats. The other group of high molecular mass hydrophobe amino acids stimulated the cell proliferation in the young and old rats brain cortex. The number of active amino acids decreased significantly in the old rats--2 times in the entodermal tissues and 4 times in the meso- and ectodermal tissues, reflecting the disturbances of the amino acid transport by aging.
Assuntos
Envelhecimento/metabolismo , Aminoácidos/metabolismo , Proliferação de Células/efeitos dos fármacos , Fatores Etários , Aminoácidos/genética , Aminoácidos/farmacologia , Animais , Apoptose , Encéfalo/citologia , Encéfalo/efeitos dos fármacos , Encéfalo/metabolismo , Código Genético , Masculino , Miocárdio/citologia , Miocárdio/metabolismo , Técnicas de Cultura de Órgãos , Pâncreas/citologia , Pâncreas/efeitos dos fármacos , Pâncreas/metabolismo , Ratos , Ratos WistarRESUMO
The effect of the cyclophosphane and amino acids with side charge radicales (basic--lysine, arginine, acid--asparagine and glutamic acid) on the cell proliferation and apoptosis development in lymphoide tissue explants in young rats (at age 3 months old) and old rats (at age 24 months old) was investigated in organotypic tissue culture. All amino acids in concentration of 10(-12) M stimulated proliferation in explants' growth zone of young rats and two amino acids (arginine, glutamic acid)--of old rats. Cyclophosphane in concentration of 1 mg/ml inhibited the explant cell proliferation. A delay of the cyclophosphane inhibitory effect was observed under the combined administration into the culture of cyclophosphane with each of the amino acids having a stimulated effect. Thus, the amino acids can possess a protective effect by action of DNA synthesis inhibitors both in young and old rats.