RESUMO
The essence of studies was that the disease is simulated in 12-day albino mice subcutaneously infected with Hantaan virus, strain 76-118 in a dose of 10 LD50. As an efficiency index, the study of drugs uses major (death protection coefficient, mean animal lifetime) and auxiliary (virological: pathogen accumulation in the brain tissues of deceased animals) parameters, biochemical (the levels of creatinine, urea, alanine aminotransferase, aspartate aminotransferase, malonic dialdehyde), hematological (count of leukocytes, leukogram) ones; as well as interferon status (the levels of circulatory interferon, leukocytic production of alpha- and gamma-interferons). The procedure for simulating the disease caused by Hantaan virus on an experimental animal is used to choose effective drugs against the pathogen of hemorrhagic nephrosonephritis.
Assuntos
Modelos Animais de Doenças , Vírus Hantaan , Febre Hemorrágica com Síndrome Renal , Camundongos , Alanina Transaminase/sangue , Animais , Animais Recém-Nascidos , Antivirais/isolamento & purificação , Antivirais/farmacologia , Aspartato Aminotransferases/sangue , Encéfalo/virologia , Creatinina/sangue , Suscetibilidade a Doenças , Avaliação Pré-Clínica de Medicamentos , Cobaias , Vírus Hantaan/efeitos dos fármacos , Febre Hemorrágica com Síndrome Renal/sangue , Febre Hemorrágica com Síndrome Renal/imunologia , Febre Hemorrágica com Síndrome Renal/virologia , Interferons/biossíntese , Dose Letal Mediana , Contagem de Leucócitos , Ativação Linfocitária , Linfócitos/imunologia , Malonatos/sangue , Coelhos , Ratos , Ureia/sangueRESUMO
The antiviral effectiveness of the combined and single use of superlow-dose amixine and virasole on the course of experimental hemorrhagic fever with renal syndrome was studied in sucking albino mice parenterally infected with their virus Hantaan. The co-administration of virasole and amixine was shown to protect 52% of the infected animals from death, which is superior to the effect of their monotherapy. The combined use of the drugs substantially prolongs the survival of albino mice after their infection and the level of brain viral reproduction suppression ( delta = 3.21 g) in the experimental group as compared to the controls and to the mice given only one of the drugs.
Assuntos
Antivirais/uso terapêutico , Vírus Hantaan , Febre Hemorrágica com Síndrome Renal/tratamento farmacológico , Ribavirina/uso terapêutico , Tilorona/uso terapêutico , Animais , Animais Lactentes , Antivirais/administração & dosagem , Encéfalo/virologia , Esquema de Medicação , Avaliação Pré-Clínica de Medicamentos , Quimioterapia Combinada , Febre Hemorrágica com Síndrome Renal/virologia , Ribavirina/administração & dosagem , Tilorona/administração & dosagemRESUMO
Efficacy of drug therapy of Omsk hemorrhagic fever (OHF) was tested experimentally using cell culture and laboratory animals infected with the OHF virus strain Ondatra. Screening tests showed that high concentrations of Virazol or interferon inducers Larifan and Rifastin caused moderately pronounced suppression of virus reproduction in cell culture. Realdiron was found to be a high-efficacy preparation causing complete inhibition of virus reproduction in cell. Larifan demonstrated the highest antiviral efficacy against OHF virus in experiments with laboratory animals. This drug prevented the death of 65% infected mice and significantly decreased infection process severity in rabbits.