Proposal of new convenient extractant for assessing phytoavailability of heavy metals in contaminated sandy soil.

The aim of the study was to compare the usefulness of 1 M HCl with aqua regia, EDTA, and CaCl2 for the extraction of phytoavailable forms of Cu, Ni, and Zn on coarse-textured soils contaminated with these metals. Two microplot experiments were used for the studies. Reed canary grass (Phalaris arundinacea), maize (Zea mays), willow (Salix viminalis), spartina (Spartina pectinata), and miscanthus (Miscanthus × giganteus) were used as test plants. They were grown on soil artificially spiked with Cu, Ni, and Zn. The experimental design included a control and three increasing doses of metals. Microplots (1 m2 × 1 m deep) were filled with sandy soil (clay-6%, pH 5.5, Corg-0.8%). Metals in the form of sulfates were dissolved in water and applied to the plot using a hand liquid sprayer. During the harvest, samples were collected from aboveground parts, roots, and the soil and then tested for their Cu, Zn, and Ni contents. The metal content of the soil was determined using four tested extractants. It was found that Cu and Ni were accumulated in roots in bigger amounts than Zn. The usefulness of the extractants was evaluated based on the correlation between the content of metals in the soil and the plant (n = 32). This study demonstrated that 1 M HCl, aqua regia, and EDTA were more efficient or equally useful for the assessment of the phytoavailability of Cu, Ni, and Zn as CaCl2. Due to the ease of performing determinations and their low cost, 1 M HCl can be recommended to assess the excess of Cu, Ni, and Zn in the coarse-textured soils.

Phytoremediation potential of Miscanthus × giganteus and Spartina pectinata in soil contaminated with heavy metals.

The aim of this work was to assess the suitability of Miscanthus × giganteus and Spartina pectinata link to Cu, Ni, and Zn phytoremediation. A 2-year microplot experiment with the tested grasses growing on metal-contaminated soil was carried out. Microplots with cement borders, measuring 1 × 1 × 1m, were filled with Haplic Luvisols soil. Simulated soil contamination with Cu, Ni, and Zn was introduced in the following doses in mg kg(-1): 0-no metals, Cu1-100, Cu2-200, Cu3-400, Ni1-60, Ni2-100, Ni3-240, Zn1-300, Zn2-600, and Zn3-1200. The phytoremediation potential of grasses was evaluated using a tolerance index (TI), bioaccumulation factor (BF), bioconcentration factor (BCF), and translocation factor (TF). S. pectinata showed a higher tolerance to soil contamination with Cu, Ni, and Zn compared to M. × giganteus. S. pectinata was found to have a high suitability for phytostabilization of Zn and lower suitability of Cu and Ni. M. × giganteus had a lower phytostabilization potential than S. pectinata. The suitability of both grasses for Zn phytoextraction depended on the age of the plants. Both grasses were not suitable for Cu and Ni phytoextraction. The research showed that one-season studies were not valuable for fully assessing the phytoremediation potential of perennial plants.

Effect of peat on the accumulation and translocation of heavy metals by maize grown in contaminated soils.

Incorporation of organic materials into soil improves the soil sorption capacity, while limiting the mobility of metals in soil and their availability to plants. These effects can be taken advantage for remediation of soils polluted with heavy metals. The objective of this study is to assess the remediatory potential of peat applied to soils with concomitant pollution with Cd, Pb, and Zn. Two 1-year experiments were run in microplots in which maize was grown as the test plant. The following treatments were compared on two soils (sandy soil and loess): (1) control, (2) heavy metals (HM), (3) HM + peat in a single dose, and (4) HM + peat in a double dose. Maize was harvested in the maturity stage; the biomass of roots and aerial parts, including grain and cobs, was measured. Besides, concentration of metals in all those plant parts and the net photosynthetic rate and transpiration rate were determined. The approach of using peat in soil remediation led to satisfactory results on sandy soil only. The application of peat to sandy soil caused significant changes in the accumulation of the metals and their translocation from roots to other parts of plants, which resulted in a higher intensity of photosynthesis and an increase in the maize biomass compared to the HM treatment.

[Parvovirus B19 DNA testing in Polish blood donors, 2004-2010]. / Badanie DNA parvowirusa B19 (B19V) u Polskich dawcoów krwi, 2004-2010.

UNLABELLED: Since 2004 Polish blood donors have been tested for parvovirus B19 (B19V) DNA. The screening testing has been performed in donors of plasma for fractionation and anti-D and anti-HBs production and donors of erythrocytes used for immunization. AIM is to present methods of the testing, quality control and results in period 2004-2010. MATERIAL AND METHODS: Testing was performed in individual donation testing (IDT) in Regional Blood Transfusion Center (RBTC) in Lublin or in pools of 24 in Institute of Haematology and Transfusion Medicine in Warsaw (IHTM). Quantitative testing with real-time PCR was preceded with nucleic acid isolation on silica based methods (Prepito Viral DNA/RNA, Chemagen and QIAamp DNA Mini Kit, QIAGEN). Amplification was performed initially with home made method and later with commercial assay (Artus Parvo B19 RG PCR Kit on Rotor Gene 6 000). In total 17 625 donations were tested: 8 539 in pools and 9 090 individually. Beside routine external quality control programmes in which both laboratories participated (Proficiency Study VQC,Amsterdam, Holand; EQA Programe, Glasgow, Scotland), panel containing negative samples, positive with very high DNA B 19V level and plasma infected with genotype 2 was prepared for RBTC in Lublin. RESULTS: B19V infection frequency was 1:980 donations, low viraemic donations were detected most frequently (1:1 037). It was identified only one donation with DNA load that could cause potential health risk for plasma product recipients (1:17 625). In one of the donors B 19V DNA was observed for 3 years and 3 months. In acute or persistent phase of infection no clinical or laboratory symptoms (morphology of peripheral blood, ALT) were observed. Due to risk of underestimation of viral load connected with viral genome polymorphism all donations with B19V positive result were not allowed to be clinically used.

[Age and effects of long-term cardiac training in patients with ischemic heart disease]. / Wiek a efekty dlugoterminowego treningu kardiologicznego u pacjentów z choroba niedokrwienna serca.

UNLABELLED: Ongoing with age decline of physical capacity reflects permanent processes of aging occurring in organism. It leads to a successive reduction of physical activity level, resulting with time in restrictions of independent life ability, which then causes anxiety and progressing social isolation. THE AIM: Evaluation of relation between age and effort tolerance in patients with chronic ischemic heart disease and comparison of cardiac rehabilitation effects in two patient groups at significantly various age. MATERIAL AND METHOD: The study comprised 103 patients (69 males and 34 females) at the mean age of 61.2 +/- 0,8 years. The patients were referred to rehabilitation because of undergone invasive treatment of IHD, using CABG (44 pts) and PTCA (48 pts), or acute coronary syndromes (11 pts). The study group was divided to the two subgroups, "A" and "B", differing significantly (p < 0.01) from each other by age. "A" group was constituted by 30 the youngest patients, with the mean age of 51.6 +/- 0.5 yrs, whereas "B" group comprised 30 the oldest patients, with the mean age of 70.9 +/- 0.6 yrs. The examined groups were comparable as to the drug treatment, clinical status, echocardiographic parameters and BMI values. During the observation period no changes in treatment and diet were made. The all patients were subjected to six-month cardiac rehabilitation, consisting of cycle ergometer training (3 times/week) and generally improving exercises (2 times/week). The parameters analyzed were the values of metabolic equivalent (MET) obtained at the initial and the final exercise treadmill test, likewise the delta of MET. RESULTS: For a population of 103 patients with IHD, the negative, statistically significant correlation Pearson's coefficient between age and MET values of initial and final exercise tests and insignificant Pearson's coefficient between age and values of MET delta were obtained. Comparison analysis of the mean MET of initial and final exercise test and the mean MET delta did not show any significant differences between the both examined "A" (young) and "B" (old) groups. CONCLUSIONS: In the examined patients with IHD, there were observed a negative, significant correlation between age and effort tolerance before and after the cardiac rehabilitation cycle, and a lack of significant correlation between age and delta of effort tolerance. There was found no considerable difference concerning a delta of effort tolerance between the patients with IHD falling into the young and the old groups.

[Hypotensive effect of 6-months cardiac rehabilitation in elderly patients]. / Efekt hipotensyjny szesciomiesiecznej rehabilitacji kardiologicznej u chorych w podeszlym wieku.

UNLABELLED: Both effective treatment of arterial hypertension and prevention from its complications are considered very important issues, especially in a population of elderly people, in whom hypertension occurs along with other risk factors, resulting in increase of incidence of myocardial infarction and stroke. THE AIM: There were assessed an influence of 6-month ambulatory cardiac rehabilitation on level of resting and peak systolic and diastolic arterial pressure in elderly patients with chronic ischemic heart disease (IHD) and arterial hypertension (AH), likewise relations between the pressure alterations and the age of the examined patients. MATERIAL AND METHOD: A group of 70 patients with IHD and AH treated invasively was subjected to a rehabilitation program. Group A consisted of 37 patients with the mean age of 69.0 +/- 0.5 years, and group B of 33 patients with the mean age of 51.5 +/- 0.5 years. The patients from the both groups differed significantly only as to the age, whereas they were comparable regarding current clinical status, echocardiographical parameters, BMI values and applied pharmacological treatment. During a follow-up period, neither drug regimen, established at least three months earlier, nor a diet were not modified. The all patients underwent a 6-month cardiac rehabilitation, combining exercises on cycle ergometers (3 times per week) and generally improving training (2 times per week). There were analyzed: the mean values of resting and of peak systolic and diastolic blood pressure at the beginning of the rehabilitation and upon completion of the program. RESULTS: Comparison of the patients from groups A and B did not reveal any significant differences concerning the initial mean values of both resting and peak pressure (systolic and diastolic). Just after the rehabilitation finishing, in A group, a statistically significant reduction of resting systolic and diastolic pressure, likewise insignificant reduction of peak systolic and diastolic pressure were observed. Contrarily, in B group, it was noted a significant reduction of resting systolic and diastolic pressure and insignificant reduction of peak diastolic pressure. In this group, yet, an insignificant increase of peak systolic pressure was found. In the both groups, there was no statistically significant correlation between the age and the delta values of resting and peak systolic and diastolic pressure. CONCLUSION: Long-term ambulatory cardiac rehabilitation caused a profitable blood pressure lowering in patients with IHD and AH, regardless of the age. A changes intensity of resting and of peak blood pressure did not show a dependence on the patients' age.

[Effect of long-term cardiac training on lipids concentration in patients with chronic heart ischemic disease treated with simvastatin]. / Wplyw dlugotrwalego treningu kardiologicznego na stezenie lipidów u chorych z przewlekla choroba niedokrwienna serca leczonych simwastatyna.

UNLABELLED: Dyslipidemia worsens a prognosis in patients with chronic heart ischemic disease, who underwent myocardial infarction. Therefore, new methods, besides drugs, are being sought, for optimizing a serum concentration of lipid fractions. THE AIM: An effect of 6-month of ambulatory long-term cardiac rehabilitation on the lipidogram fractions concentration in patients with chronic heart ischemic disease treated with simvastatin, as well as a correlation between lipids changes and cardiac training intensity was assessed. MATERIAL AND METHOD: Rehabilitation was performed in 66 patients with previous myocardial infarction treated invasively (27 CABG and 39 PTCA), who constituted group I. A control group (group II) consisted of 32 patients with previous myocardial infarction also treated invasively (24 CABG and 8 PTCA), but not subjected to rehabilitation. The two analyzed groups did not differ significantly from each other as to age, applied drug regimen, current clinical status, echocardiographic parameters and BMI values. Group I was subjected to 6-month cardiac rehabilitation program, comprising 45-minute training on cycle ergometer (three times per week) and generally improving exercises (2 times per week). Blood concentration of lipidogram fractions was assessed: total cholesterol (TC), HDL- and LDL-cholesterol, and triglicerides (TG) at the onset and upon completion of the rehabilitation cycle. RESULTS: The both patient groups were comparable concerning the initial concentration of the lipid fractions. After finishing the rehabilitation program, in the group I, a statistically significant reduction of TC, LDL and TG concentration was found out. In addition, a significant increase of HDL concentration was noted. In contrary, in the group II (without rehabilitation), the only significant change concerned a concentration of HDL fraction, which decreased. Furthermore, in the group I, it was noted a negative, statistically significant correlation between intensity of cardiac training, expressed by training workload and delta of work, and a difference in blood concentration of triglicerides, measured just before the training onset and after the program was finished. CONCLUSION: It was revealed that long-term ambulatory cardiac rehabilitation has a profitable effect on serum concentration of the all lipid fractions in patients with chronic heart ischemic disease cured with simvastatin, regardless of training intensity. It was also ascertained that an extent of changes in triglicerides serum concentration was related to an intensity of the cardiac training applied to the patients.

[Interrelation between arterial hypertension and body mass index in patients with concomitant ischemic heart disease treated with cardiac rehabilitation]. / Zaleinosc cisnienia tetniczego od indeksu masy ciala u pacjentów z choroba niedokrwienna serca poddanych rehabilitacji kardiologicznej.

A goal of the study was to investigate the interrelations between body mass index (BMI) and arterial pressure level and its alterations induced by a long-term cardiac rehabilitation. In the patients with arterial hypertension with concomitant ischemic heart disease (IHD). The study group consisted of 73 patients (46 males and 27 females), with age range 54-64 years (the mean: 59.59 +/- 0.67 years), subjected to the 6-month ambulatory supervised cardiac rehabilitation, which comprised stamina training with cycloergometer combined with generally improving exercises. Arterial pressure level was measured in the resting state (just before the training onset) and at the interval training peak (peak pressure) during the consecutive three initial and three final training sessions. Upon completion of the rehabilitation cycle, statistically significant reduction of resting systolic pressure: 116.85 +/- 1.68 mmHg vs 105.12 +/- 1.43 mmHg (p<0.01) and insignificant reduction of resting diastolic pressure 79.85 +/- 1.24 mmHg vs 77.44 +/- 1.21 mmHg (NS) were observed. Additionally, insignificant lowering both systolic and diastolic peak pressure was noted, respectively: 119.45 +/- 1.92 vs 118.75 +/- 2.3 mmHg and 78.63 +/- 1.22 mmHg vs 76.96 +/- 1.30 mmHg. Correlation analysis concerning the values of resting systolic and diastolic pressure, likewise of peak systolic and diastolic pressure, on the one hand, and the values of body mass index, on the other hand, yielded in the all considered cases the statistically insignificant positive Pearson's coefficients. The further dependence analyzed, between the delta of resting and peak pressure, systolic and diastolic, and the body mass index values was featured by the insignificant statistically negative correlation coefficients. In result of the 6-month rehabilitation, 36 out of 73 patients demonstrated BMI value reduction by 0.65 +/- 0.33, which did not affect significantly an extent of the arterial pressure lowering. Therefore, in the examined patient group with hypertension and IHD, body mass index turned out to determine neither the initial nor the after-rehabilitation pressure levels, both systolic and diastolic.