[Comparative immunochemical and immunoallergic characteristics of staphylococci of different pathogenicity. II. Diagnostic value of extracts of Staphylococcus aureus and Staphylococcus epidermidis in delayed type allergic reactions]. / SRavitel'naia immunokhimicheskaia i immunoallergologicheskaia kharakteristika stafilokokkov razlichnoi patogennosti. Soobshchenie II. Diagnosticheskaia tsennost' razlichnykh ékstraktov Staphylococcus aureus i Staphylococcus epidermidis v allergicheskikh reaktsiiakh zamedlennogo tipa.

A total of 20 different allergens extracted from various staphylococcal strains were studied in the delayed hypersensitivity reactions in experiments on guinea-pigs. The most intensive dermatoallergic reactions were observed after the injection of the cytoplasmic fraction, acidic and alkaline extracts; reactions to the allergen of Ando-Verzhikovsky were less pronounced. The cytoplasmic fraction and the allergen of ando-Verzhikovsky were more active in the reaction of leukocyte migration inhibition in the blood. Both common and individual allergenic components were detected. No correlation between the results of the immunochemical and allergological studies of staphylococcal extracts was revealed.

[Comparative immunochemical and immunoallergologic characteristics of staphylococci varying in pathogenicity. I. Immunochemical characteristics of the antigenic composition of S. aureus and S. epidermidis]. / Sravnitel'naia immunokhimicheskaia i immunoallergologicheskaia kharakteristika stafilokokkov razlichnoi patogennosti. Soobshchenie I. Immunokhimicheskaia kharakteristika antigennogo sostava S. aureus i S. epidermidis.

Chemical extracts and fractions prepared from pathogenic and nonpathogenic staphylococcal strains were studied with immunodiffusion. Antigens were detected reacting with both homologous and heterologous antisera against the intact coccal cells. The allergens obtained by acidic and alkaline extraction were highly active antigens. The preparation of S. epidermidis obtained by the method of Ando-Verzhikovsky had the lowest antigen level.

[Increased streptomycin sensitivity and its associations with microbial allergy]. / Izuchenie povyshennoi chuvstvitel'nosti k streptomitsinu i sochetanii ee s mikrobnoi allergiei.

Regularities of streptomycin hypersensitivity development and its association with microbial allergy were studied on 75 guinea pigs. A model of retarded allergy was obtained by the animal sensitization with streptomycin in doses of 20 000 gamma per 1 kg of the body weight. Two procedures were used for the animal sensitization, i.e. with the use of the Freund adjuvant or without it. Positive skin-allergic tests to streptomycin (mainly 24-hour) were registered 2 weeks after discontinuation of the sensitization and persisted for the whole observation period (up to 6--8 weeks). The tests for the leucocyte migration were also positive and precipitating antibodies were found in the serum according to the Hoigné method. Simultaneous sensitization with streptomycin and staphylococci resulted in some suppression of the development of retarded hypersensitivity to the microbial antigen. Subsequent sensitization at first with staphylococci and then with streptomycin favoured a mutual increase in the allergic reconstruction to both antigens. The histomorphological studies confirmed the data of the immunoallergological examination.