RESUMO
In current clinical practices related to orthopedics, dental, and cardiovascular surgeries, a number of biomaterial coatings, such as hydroxyapatite (HAp), diamond-like carbon (DLC), have been used in combination with metallic substrates (stainless steel, Ti6Al4V alloy, etc.). Although SiBCN coatings are widely explored in material science for diverse applications, their potential remains largely unexplored for biomedical applications. With this motivation, the present work reports the development of SiBxCyNzOm coatings on a Ti6Al4V substrate, employing a reactive radiofrequency (RF) magnetron sputtering technique. Three different coating compositions (Si0.27B0.10C0.31N0.07O0.24, Si0.23B0.06C0.21N0.22O0.27, and Si0.20B0.05C0.19N0.20O0.35) were obtained using a Si2BC2N target and varying nitrogen flow rates. The hydrophilic properties of the as-synthesized coatings were rationalized in terms of an increase in the number of oxygen-containing functional groups (OH and NO) on the surface, as probed using XPS and FTIR analyses. Furthermore, the cellular monoculture of SVEC4-10 endothelial cells and L929 fibroblasts established good cytocompatibility. More importantly, the coculture system of SVEC4-10 and L929, in the absence of growth factors, demonstrated clear cellular phenotypical changes, with extensive sprouting leading to tube-like morphologies on the coating surfaces, when stimulated using a customized cell stimulator (StimuCell) with 1.15 V/cm direct current (DC) electric field strength for 1 h. In addition, the hemocompatibility assessment using human blood samples revealed clinically acceptable hemolysis, less erythrocyte adhesion, shorter plasma recalcification, and reduced risk for thrombosis on the SiBxCyNzOm coatings, when compared to uncoated Ti6Al4V. Taken together, the present study unambiguously establishes excellent cytocompatibility, hemocompatibility, and defines the preangiogenic properties of SiBxCyNzOm bioceramic coatings for potential biomedical applications.
Assuntos
Ligas , Materiais Revestidos Biocompatíveis , Teste de Materiais , Titânio , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacologia , Ligas/química , Ligas/farmacologia , Titânio/química , Titânio/farmacologia , Humanos , Animais , Camundongos , Células Endoteliais/efeitos dos fármacos , Células Endoteliais/citologia , Linhagem Celular , Propriedades de Superfície , Fibroblastos/efeitos dos fármacos , Fibroblastos/citologia , Neovascularização Fisiológica/efeitos dos fármacosRESUMO
Silicon carbonitride films were deposited on Si (100), Ge (111), and fused silica substrates through the reactive magnetron sputtering of a SiC target in an argon-nitrogen mixture. The deposition was carried out at room temperature and 300 °C and at an RF target power of 50-150 W. An increase in the nitrogen flow rate leads to the formation of bonds between silicon and carbon atoms and nitrogen atoms and to the formation of SiCxNy layers. The as-deposited films were analyzed with respect to their element composition, state of chemical bonding, mechanical and optical properties, and wetting behavior. It was found that all synthesized films were amorphous and represented a mixture of SiCxNy with free carbon. The films' surfaces were smooth and uniform, with a roughness of about 0.2 nm. Depending on the deposition conditions, SiCxNy films within the composition range 24.1 < Si < 44.0 at.%, 22.4 < C < 56.1 at.%, and 1.6 < N < 51.9 at.% were prepared. The contact angle values vary from 37° to 67°, the hardness values range from 16.2 to 34.4 GPa, and the optical band gap energy changes from 1.81 to 2.53 eV depending on the synthesis conditions of the SiCxNy layers. Particular attention was paid to the study of the stability of the elemental composition of the samples over time, which showed the invariance of the composition of the SiCxNy films for five months.
RESUMO
The data set covers the results of a study of 96 samples of peat bog soil from the fall place of the first stage of the Cyclone-3 launch vehicle contained unburned toxic rocket fuel 1,1-dimethylhydrazine (UDMH) in the European North of Russia. Soil samples were taken during a helicopter expedition to the "Koida" fall region of Plesetsk Cosmodrome operation zone in October 2015 at different distances from the center of the fall site and from different soil horizons. Samples were analyzed by liquid chromatography with amperometric detection and gas chromatography - tandem mass spectrometry. The contents of UDMH and the ten most important products of its transformations (methylhydrazine, hydrazine, 1,1,4,4-tetramethyltetrazene, formaldehyde, acetaldehyde and furaldehyde N,N-dimethylhydrazones, 1-formyl-2,2-dimethylhydrazine, N,N-dimethylformamide, N-nitrosodimethylamine, and 1-methyl-1H-1,2,4-triazole) were determined. The obtained data reflect the spatial distribution, migration and transformation of UDMH in the fall places of rocket stages under conditions of subarctic which is discussed in related research article "Migration and transformation of 1,1-dimethylhydrazine in peat bog soil of rocket stage fall site in Russian North" [1]. They can be further used for understanding the UDMH transformation pathways in soils rich in organic matter and assessment of environmental impact of space rocket activities in high latitudes.
RESUMO
An ingress of highly toxic rocket fuel 1,1-dimethylhydrazine (UDMH) and its transformation products into environment represents a serious negative impact on the ecosystem, as well as human health. The present research demonstrates the first data on the spatial distribution and quantification of UDMH and its main transformation products (methylhydrazine, hydrazine, 1,1,4,4-tetramethyltetrazene, formaldehyde, acetaldehyde and furaldehyde N,N-dimethylhydrazones, 1-formyl-2,2-dimethylhydrazine, N,N-dimethylformamide, N-nitrosodimethylamine, and 1-methyl-1H-1,2,4-triazole) in the peat bog soil of the fall site in subarctic region. One hundred samples of peat bog soil and one sample of surface water were analyzed by the developed earlier methodology. The considerable amounts of UDMH and most of its transformation products were found at distances of not >10 m from the center of the fall site. The maximum concentration of UDMH was found near the center, where maximal permissible concentration (MPC) was exceeded 2400-fold. The greatest pollution was observed in the surface soil layer, while methylhydrazine, 1-methyl-1H-1,2,4-triazole, 1-formyl-2,2-dimethylhydrazine, formaldehyde and acetaldehyde N,N-dimethylhydrazones, and N,N-dimethylformamide were the major UDMH transformation products. With increasing distance from the center, the composition of the transformation products changes in favor of the last three compounds. Formaldehyde N,N-dimethylhydrazone and N,N-dimethylformamide are present in all soil samples and can be considered as reliable markers of contamination with rocket fuel. The surface water of the peat bog contained four UDMH transformation products in considerable concentrations, including extremely toxic N-nitrosodimethylamine. The processes of migration and transformation of UDMH in peat bog soil differ considerably from those in sandy soils. This is due to cold climate of subarctic zone, the reducing environment of peat bog, and strong binding of hydrazines to organic matter of peat, which prevents migration of pollutants and contributes to the long-term maintenance of high levels of soil pollution.
RESUMO
Treatment of cancer patients remains a serious medical problem and the development of alternative treatment strategies is therefore of great importance. In this connection, we developed a new bacterial-based, anticancer method. Ten cancer patients (three males, seven females) were involved in this study. Bacterial suspension of stationary phase Bacillus oligonitrophilus KU-1 was used as a remedy for peroral administration. In five patients, side effects (sicchasia, slight blood, and intracranial pressure gain) were detected, but all patients showed significant delay of lethal outcome without serious side effects. In conclusion, the suggested method was, in our opinion, a good alternative to conventional chemo- and radiotherapy techniques. In order to evaluate its efficiency for various tumors, a double-blind, placebo-controlled, multicenter study is needed.
Assuntos
Antineoplásicos/administração & dosagem , Bacillus , Neoplasias/mortalidade , Neoplasias/terapia , Probióticos/administração & dosagem , Administração Oral , Adulto , Idoso , Antineoplásicos/uso terapêutico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias/microbiologia , Probióticos/uso terapêutico , Taxa de Sobrevida , Fatores de TempoRESUMO
Chemotherapy and radiotherapy remain mostly palliative methods for metastatic cancer treatment. Limitations in efficacy and safety of established treatments continue to underline the need for improved treatments for malignancy. Results with some probiotics with antitumor activity have been promising. Here, we report that oral reception of Bacillus oligonitrophilus KU-1 resulted in the prolongation of lives in cancer patients with terminal prognosis and stabilization of cancer growth. The theoretical basis for the phenomena observed is discussed.