[Assesment of the specific oxidase activity of ceruloplasmin in pregnant women].

The specific oxidase activity of ceruloplasmin (activity per unit mass of enzyme protein) was studied in plasma of pregnant women with gestosis, feto-placental insufficiency, postmature and in normal pregnancy. The specific oxidase activity decreased in all groups with pathology. The ceruloplasmin concentration decreased in the postmature group.

[Dynamics of oxidase activity in blood plasma upon intravenous and intramuscular administration of ceruloplasmin]. / Dinamika izmeneniia oksidaznoi aktivnosti plazmy pri razlichnikh sposobakh vvedeniia preparata Tseruplazmin.

Oxidase activity dynamics in the blood plasma of test animals was studied upon intravenous and intramuscular injections of ceruloplasmin. It was found that the enzymatic activity does not reflect the content of drug in the blood plasma and is not always correlated with the amount of exogenously introduced drug. Intramuscular injections can also be used for the administration of ceruloplasmin.

[Antioxidant and antihypoxic effects of the ceruloplasmin preparation in the hypobaric hypoxia model]. / Izuchenie antioksidantnogo i antigipoksicheskogo deistviia preparata tseruloplazmin na modeli gipobaricheskoi gipoksii.

The antioxidant and antihypoxant effects of the drug ceruloplasmin were studied on an experimental model of hypobaric hypoxia in rats. Two forms of the drug characterized by low and high enzymatic activity were administered (intramuscularly or intraperitoneally) 20 min or 1 day before hypobaric hypoxia test. The drug effect was independent of the enzymatic activity level. The maximum activity of ceruloplasmin was observed when the drug was administered one day before test. Analyses showed that the drug activated superoxide dismutase, while not changing the catalase activity.

[Modeling of vitreoretinal proliferation]. / Modelirovanie vitreoretinal'noi proliferatsii.

Complexes of endogenous immunopeptides produced by autologous peripheral blood lymphocytes were injected to the vitreous body of 17 chinchilla rabbits (34 eyes). The agents injected were autosupernatant. L fraction containing molecular substances with mol. weight of 40,000 to 60,000 D, and M fraction containing substances with mol. weight of 15,000 to 30,000 D. Clinical and morphologic studies demonstrated that injection of endogenous immunopeptides led to active lymphoid inflammatory infiltration associated with proliferation and formation of new vessels. In contrast to the M fraction, the exudative processes triggered by the L fraction were more definitely localized and associated with the appearance of fibroplastic cords (the proliferative component). The models created may be used for the development of methods for prevention and treatment of vitreoretinal proliferation.

Natural complex of cytokines is a potent stimulant to posttraumatic regeneration in rabbit cornea.

We elaborated an original technique based on local application of natural complex of cytokines (NCC) secreted by autologous peripheral leukocytes. In this study, we evaluated NCC influence on the healing of penetrating corneal wounds. NCC was derived from supernatants of PHA-stimulated rabbit peripheral leukocytes. Biological tests revealed the presence of IL-1, TNF, IL-6, MIF, and LIF in the complex. Chinchilla rabbits with standard penetrating corneal wounds received daily NCC instillation. The controls were instilled with cultural media 199 with antibiotics. At 24 hours, 3, 7, 14 and 30 days post-wounding, the rabbits were euthanized. We performed morphometry of corneal cross-sections stained with hematoxylin and eosin. In NCC-treated animals, we observed more vigorous migration and activation of neutrophils and macrophages followed by augmented resorption of fibrin. The later post-injury period (14-30 days) was marked with complete healing of the endothelial defect (in the controls, the mass of proliferating cells projected into the anterior eye chamber) and mature scar tissue with a higher content of fibrous component. NCC-treated eye scars were 1.6-fold thinner than the controls'. Local application of NCC promotes effective healing of posttraumatic cornea. It regulates all stages of regeneration and prevents rude scarring.

[Mechanism regulating healing of perforating wounds of the cornea by natural cytokine complex]. / Mekhanizm reguliatsii zazhivleniia pronikaiushchikh ranenii rogovitsy s pomoshch'iu estestvennogo kompleksa tsitokinov.

Effects of a natural complex of cytokines secreted by peripheral blood leukocytes on the healing of perforating wounds of the cornea were studied in 28 rabbits (14 animals in control and 14 in experimental groups). The process of healing of similar cut wounds of the cornea during local application of cytostatics was assessed by morphological and morphometric methods on days 1, 3, 7, 14, and 30 after the injury. Two periods, early and late, were distinguished in the morphogenesis of healing of a cut wound of the cornea under the effect of natural cytokines. The cytokines enhanced neutrophil migration to the wound canal in the early period of morphogenesis, this accelerating fibrin resorption, which was followed by filling of the wound canal with keratoblastic proliferate. In the late period of morphogenesis of wound healing (days 14-30) the functional activity of fibroblasts was increased, this being conducive to activation of collagenization of cicatricial tissue and resorption of the cicatrix as a result of a possible increase of collagenase release under the effect of cytokines. The possible mechanism of regulating effect of cytokines on corneal repair is discussed.

[The natural cytokine complex in treatment of penetrating wounds of the rabbit cornea in an experiment]. / Estestvennyi kompleks tsitokinov v lechenii pronukaiushchikh ranenii rogovitsy glaza krolika v éksperimente.

The influence of cytokine complex and its fractions, produced by peripheral blood mononuclear cells on the healing of rabbit corneal wounds has been investigated. The cytokine complex affected on the early stage of wound healing, and was more effective than fractions of this complex. The delay of regeneration and as a result--formation of more thin scar have been observed.