Analysis and effective separation of toxic pollutants from water resources using MBBR: Pathway prediction using alkaliphilic P. mendocina.

Azo dyes are highly toxic, which acts as a notable mutagen and carcinogen. This has a significant effect on human health, plants, animals, aquatic and terrestrial environments. Thus, the degradation of the azo dyes is exclusively studied using the conventional methods of which biodegradation is an eco-friendly approach. Hence, the present study is focused on the elucidation of reactive mixed azo dye degradation pathway using MBBR and laccase enzyme produced by an alkaliphilic bacterium P. mendocina. Synthetic wastewater treatment performed using MBBR was very effective which reduced the COD and BOD to 90 mg/L and 460 mg/L. The potential degrader P. mendocina was isolated and laccase enzyme was screened. Finally, the degradation pathway was elucidated. The in silico toxicity analysis predicted Reactive Red and Reactive Brown as developmental toxicants during Reactive Black as Developmental non-toxicant. Docking studies were performed to understand interaction of laccase with compounds evolved from dyes.

Decolorization of azo dyes (Direct Blue 151 and Direct Red 31) by moderately alkaliphilic bacterial consortium

Abstract Removal of synthetic dyes is one of the main challenges before releasing the wastes discharged by textile industries. Biodegradation of azo dyes by alkaliphilic bacterial consortium is one of the environmental-friendly methods used for the removal of dyes from textile effluents. Hence, this study presents isolation of a bacterial consortium from soil samples of saline environment and its use for the decolorization of azo dyes, Direct Blue 151 (DB 151) and Direct Red 31 (DR 31). The decolorization of azo dyes was studied at various concentrations (100–300 mg/L). The bacterial consortium, when subjected to an application of 200 mg/L of the dyes, decolorized DB 151 and DR 31 by 97.57% and 95.25% respectively, within 5 days. The growth of the bacterial consortium was optimized with pH, temperature, and carbon and nitrogen sources; and decolorization of azo dyes was analyzed. In this study, the decolorization efficiency of mixed dyes was improved with yeast extract and sucrose, which were used as nitrogen and carbon sources, respectively. Such an alkaliphilic bacterial consortium can be used in the removal of azo dyes from contaminated saline environment.

Decolorization of azo dyes (Direct Blue 151 and Direct Red 31) by moderately alkaliphilic bacterial consortium.

Removal of synthetic dyes is one of the main challenges before releasing the wastes discharged by textile industries. Biodegradation of azo dyes by alkaliphilic bacterial consortium is one of the environmental-friendly methods used for the removal of dyes from textile effluents. Hence, this study presents isolation of a bacterial consortium from soil samples of saline environment and its use for the decolorization of azo dyes, Direct Blue 151 (DB 151) and Direct Red 31 (DR 31). The decolorization of azo dyes was studied at various concentrations (100-300mg/L). The bacterial consortium, when subjected to an application of 200mg/L of the dyes, decolorized DB 151 and DR 31 by 97.57% and 95.25% respectively, within 5 days. The growth of the bacterial consortium was optimized with pH, temperature, and carbon and nitrogen sources; and decolorization of azo dyes was analyzed. In this study, the decolorization efficiency of mixed dyes was improved with yeast extract and sucrose, which were used as nitrogen and carbon sources, respectively. Such an alkaliphilic bacterial consortium can be used in the removal of azo dyes from contaminated saline environment.