Oxidative stress and DNA damage in horses naturally infected with Theileria equi.

The aim of this study was to determine the concentrations of oxidative stress parameters and DNA damage in horses infected by Theileria equi. Initial screening of 110 horses with duplex PCR enabled the selection of 30 infected horses with T. equi and 30 free of infection (control). Specimens from the 60 horses were further analysed by determining the following oxidative stress parameters: extent of haemolysis (EH), plasma free haemoglobin (PHb), catalase (CAT), Cu,Zn superoxide dismutase (SOD1), paraoxonase (PON1), nitrite (NO2-), total nitrate and nitrite (NOx), malondialdehyde (MDA) and free thiol groups (-SH). In addition, relative distribution of lactate dehydrogenase (LDH1-LDH5) activity and the DNA-damaging effects of T. equi infection were evaluated. Compared to control horses, horses infected with T. equi had significantly higher SOD1 activities (P <0.05) and PHb (P <0.01), NO2- (P <0.001), NOx (P <0.05) and MDA concentrations (P <0.001), and significantly lower EH (P <0.001), CAT (P <0.01) and PON1 (P <0.001) activities, and thiol group concentrations (P <0.05). The comet assay demonstrated significantly increased DNA damage in T. equi infected cells compared to non-infected cells (P <0.001). Infected horses had significantly increased LDH5 isoenzyme activities (P <0.05). There was higher production of ROS/RNS in T. equi-infected horses, which resulted in changes in osmotic fragility, damage to lipids, proteins and DNA, haemolysis and hepatocellular damage. Oxidative stress in horses naturally infected with T. equi could contribute to the pathogenesis of the infection.

Age-related changes in the articular cartilage of the stifle joint in non-working and working German Shepherd dogs.

The aims of this study were to define age-related histological changes in the articular cartilage of the stifle joint in non-chondrodystrophic dogs and to determine whether physical activity has a positive impact on preservation of cartilage structure during ageing. Twenty-eight German shepherd dogs were included in the study. These dogs had no evidence of joint inflammation as defined by clinical assessment, radiology and synovial fluid analysis (specifically absence of synovial fluid serum amyloid A). The dogs were grouped as young working (n » 4), young non-working (n » 5), aged working (n » 13) and aged non-working (n » 6) animals. Gross changes in the stifle joints were recorded and biopsy samples of femoral and tibial articular cartilage were evaluated for thickness; chondrocyte number, density, surface area and morphology; isogenous group morphology; tidemark integrity; subchondral bone structure; presence of proteoglycans/ glycosaminoglycans; and expression of type I, II and X collagens. The major age-related changes, not related to type of physical activity, included elevated chondrocyte density and thinning of tibial cartilage and increased chondrocyte surface area in the superficial and intermediate zone of the femoral cartilage. There was also expression of type X collagen in the femoral and tibial calcified and non-calcified cartilage; however, type X collagen was not detected in the superficial zone of old working dogs. Therefore, ageing, with or without physical activity, leads to slight cartilage degeneration, while physical activity modulates the synthesis of type X collagen in the superficial cartilage zone, partially preserving the structure of hyaline cartilage.

Relationship between overweight, obesity and socioeconomic factors of adolescents in Vojvodina, Serbia.

Obesity is a very important issue in developed societies and depends on many factors. The aim of this paper was to determine a possible relationship between overweight, obesity and socioeconomic factors among adolescents in Vojvodina. A cross-sectional anthropometric study was carried out from 2001 to 2004 in towns of Vojvodina, northern Serbia. The research included height and body weight measurements as well as body mass index (BMI) of 1236 schoolboys and 1414 schoolgirls aged 15-18 years. The socioeconomic factors (SES) included parents' level of education and monthly income per family member. The overweight prevalence of about 10% (8595) were recorded in both male and female adolescents. A significant correlation (p<0.01) between all socioeconomic factors was observed in male subjects, although there were no significant differences in the prevalence of overweight and obesity when different categories of subjects set by SES were compared. In females lower rates of the prevalence of overweight and obesity were detected in the subjects whose parents had a university education, but the difference was not significant. Regarding the prevalence of obesity, a significant difference was recorded between females with high and low incomes, those with a high income showing significantly greater prevalence of obesity than females coming from low income families. The results of the research indicate that in Vojvodina the family income is the only factor that significantly correlates with female obesity.

Momentum transfer from quantum vacuum to magnetoelectric matter.

A recent publication [Phys. Rev. Lett. 92, 020404 (2004)] raises the possibility of momentum transfer from zero-point quantum fluctuations to matter, controlled by applied electric and magnetic fields. We present a Lorentz-invariant description using field-theoretical regularization techniques. We find no momentum transfer for homogeneous media, but predict a very small transfer for a Casimir-type geometry.

Body height and weight of children in Novi Sad.

BACKGROUND: Height and weight are the most important measures of growth and development and indirect indicators of living conditions. The socio-economic conditions in Serbia have varied drastically. In 1990, a political and economic crisis started causing a rapid fall of standards of living. AIM: The study aimed to determine the height and weight of children aged 3-11 in the periods between 1971, 1981, 1991 and 2001 in Novi Sad, Serbia. SUBJECTS AND METHODS: This investigation was carried out in the same primary schools and pre-school institutions. The data included children of Serbian nationality born in Novi Sad whose parents were also born there and had the same socio-economic background. RESULTS: Positive changes in height and weight were recorded in the decades 1971-1981 and 1981-1991, except for the height of 8-year-old children. In the period 1991-2001, an increase in height was observed only at the age of 8 in boys and until the age of 9 in girls. As for weight, an increase was recorded in 9-year-old boys, while in girls it was present at all ages except for the ages of 7 and 10. Considering the period 1971-2001, positive changes in height were recorded from the age of 6 in boys and 5 in girls. The changes in weight were positive at all ages except the age of 5 in boys and after the age of 6 in girls. CONCLUSION: Lower values of height and weight recorded in 2001 are probably due to the changes in living conditions or they indicate that the acceleration reached its peak.

Jackson-Weiss syndrome registered in four successive generations. The facies of Crouzon's syndrome with foot abnormalities.

A family with 15 individuals in four successive generations affected by Jackson-Weiss syndrome, craniosynostosis with Crouzon-variant-like phenotype and feet's abnormalities, is presented. An autosomal dominant inheritance pattern with complete penetrance, variable expressivity, and wide intrafamilial variation, more among, less within the same generation, was observed. Concerning the frequency and severity of complications, the evolution of craniofacial deformities seems to parallel those described with Crouzon syndrome, suggesting the similar evaluation and management.