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Injury, tumors, ischemia, and lesions in the cerebellum show the involvement of this region in human speech. The association of the cerebellum with learned birdsong has only been identified recently. Cerebellar dysfunction in young songbirds causes learning disabilities, but its role in adult songbirds has not been established. The aim of this study was to investigate the role of the deep cerebellar nuclei (DCN) in adult birdsong. We created bilateral excitotoxic lesions in the DCN of adult male zebra finches (Taeniopygia guttata) and recorded their songs for up to 4 months. Using magnetic resonance imaging (MRI) and immunohistochemistry, we validated the lesion efficacy. We found that the song duration significantly increased from 14 weeks post-op; the increase in duration was caused by a greater number of introductory notes as well as a greater number of syllables sung after the introductory notes. On the other hand, the motif duration decreased from 8 weeks after DCN lesions were induced, which was due to faster singing of syllables, not changes in inter-syllable interval length. DCN lesions also caused a decrease in the fundamental frequency of syllables. In summary, we showed that DCN lesions influence the temporal and acoustic features of birdsong. These results suggest that the cerebellum influences singing in adult songbirds.
Assuntos
Tentilhões , Aves Canoras , Animais , Masculino , Cerebelo/diagnóstico por imagem , Comunicação , Aprendizagem , Vocalização AnimalRESUMO
We have studied the effects of dopamine antagonists and agonists on Japanese quail behavior in the spatial judgment task. Twenty-four Japanese quail hens were trained in the spatial discrimination task to approach the feeder placed in the rewarded location (Go response, feeder containing mealworms) and to not approach the punished location (No-Go response, empty feeder plus aversive sound). In a subsequent spatial judgment task, the proportion of Go responses as well as approach latencies to rewarded, punished, and three ambiguous locations (near-positive, middle, near-negative, all neither rewarded nor punished) were assessed in 20 quail hens that successfully mastered the discrimination task. In Experiment 1, each bird received five treatments (0.1 and 1.0 mg/kg of dopamine D1 receptor antagonist SCH 23390, 0.05 and 0.5 mg/kg of dopamine D2 receptor antagonist haloperidol, and saline control) in a different order, according to a Latin square design. All drugs were administered intramuscularly 15 min before the spatial judgment test, with 2 days break between the treatments. Both antagonists caused a significant dose-dependent increase in the approach latencies as well as a decrease in the proportion of Go responses. In Experiment 2, with the design analogous to Experiment 1, the hens received again five treatments (1.0 and 10.0 mg/kg of dopamine D1 receptor agonist SKF 38393, 1.0 and 10.0 mg/kg of dopamine D2 receptor agonist bromocriptine, and saline control), applied intramuscularly 2 h before the test. The agonists did not have any significant effect on approach latencies and the proportion of Go responses in the spatial judgment task, as compared to the saline control, except for 10.0 mg/kg SKF 38393, which caused a decrease in the proportion of Go responses. The approach latency and the proportion of Go responses were affected by the cue location in both experiments. Our data suggest that the dopamine D1 and D2 receptor blockade leads to a decrease in the reward expectation and the negative judgment of stimuli. The effect of dopamine receptor activation is less clear. The results reveal that dopamine receptor manipulation alters the evaluation of the reward and punishment in the spatial judgment task.
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The striatal region Area X plays an important role during song learning, sequencing, and variability in songbirds. A previous study revealed that neurotoxic damage within Area X results in micro and macrostructural changes across the entire brain, including the downstream dorsal thalamus and both the upstream pallial nucleus HVC (proper name) and the deep cerebellar nuclei (DCN). Here, we specify these changes on cellular and gene expression levels. We found decreased cell density in the thalamic and cerebellar areas and HVC, but it was not related to neuronal loss. On the contrary, perineuronal nets (PNNs) in HVC increased for up to 2 months post-lesion, suggesting their protecting role. The synaptic plasticity marker Forkhead box protein P2 (FoxP2) showed a bi-phasic increase at 8 days and 3 months post-lesion, indicating a massive synaptic rebuilding. The later increase in HVC was associated with the increased number of new neurons. These data suggest that the damage in the striatal vocal nucleus induces cellular and gene expression alterations in both the efferent and afferent destinations. These changes may be long-lasting and involve plasticity and neural protection mechanisms in the areas directly connected to the injury site and also to distant areas, such as the cerebellum.
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There are two most heavily used markers of cell proliferation, thymidine analogues 5-bromo-2'-deoxyuridine (BrdU) and 5-ethynyl-2'-deoxyuridine (EdU) that are incorporated into the DNA during its synthesis. In neurosciences, they are often used consecutively in the same animal to detect neuronal populations arising at multiple time points, their migration and incorporation. The effectivity of these markers, however, is not well established. Here, we studied the effectivity of equimolar doses of BrdU and EdU to label new cells and looked for the dose that will label the highest number of proliferating cells in the neurogenic ventricular zone (VZ) of adult songbirds. We found that, in male zebra finches (Taeniopygia guttata), the equimolar doses of BrdU and EdU did not label the same number of cells, with BrdU being more effective than EdU. Similarly, in liver, BrdU was more effective. The saturation of the detected brain cells occurred at 50 mg/kg BrdU and above 41 mg/kg EdU. Higher dose of 225 mg/kg BrdU or the equimolar dose of EdU did not result in any further significant increases. These results show that both markers are reliable for the detection of proliferating cells in birds, but the numbers obtained with BrdU and EdU should not be compared.
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Neurogenesis takes part in the adult songbird brain and new neurons are integrated into the forebrain including defined areas involved in the control of song learning and production. It has been suggested that the new neurons in the song system might enable vocal variability. Here, we examined the basal levels of neurogenesis in two songbird species, zebra finch ( Taeniopygia guttata) and Bengalese finch ( Lonchura striata var. domestica), which do not learn new song elements as adults but differ in the level of song sequence variability. We found that Bengalese finches had less linear and stereotyped song sequence and a higher number of newborn cells in the neurogenic subventricular zone (SVZ) as well as the number of newly born neurons incorporated into the vocal nucleus HVC (used as a proper name) in comparison to zebra finches. Importantly, this vocal sequence variability in Bengalese finches correlated with the number of new neurons in the vocal nucleus HVC and more plastic song was associated with higher neuronal incorporation. In summary, our data support the hypothesis that newly generated neurons facilitate behavioural variability.
Assuntos
Neurogênese , Neurônios/fisiologia , Aves Canoras/fisiologia , Vocalização Animal/fisiologia , Animais , Tentilhões/fisiologia , MasculinoRESUMO
Similar to human speech, bird song is controlled by several pathways including a cortico-basal ganglia-thalamo-cortical (C-BG-T-C) loop. Neurotoxic disengagement of the basal ganglia component, i.e. Area X, induces long-term changes in song performance, while most of the lesioned area regenerates within the first months. Importantly however, the timing and spatial extent of structural neuroplastic events potentially affecting other constituents of the C-BG-T-C loop is not clear. We designed a longitudinal MRI study where changes in brain structure were evaluated relative to the time after neurotoxic lesioning or to vocal performance. By acquiring both Diffusion Tensor Imaging and 3-dimensional anatomical scans, we were able to track alterations in respectively intrinsic tissue properties and local volume. Voxel-based statistical analyses revealed structural remodeling remote to the lesion, i.e. in the thalamus and, surprisingly, the cerebellum, both peaking within the first two months after lesioning Area X. Voxel-wise correlations between song performance and MRI parameters uncovered intriguing brain-behavior relationships in several brain areas pertaining to the C-BG-T-C loop supervising vocal motor control. Our results clearly point to structural neuroplasticity in the cerebellum induced by basal ganglia (striatal) damage and might point to the existence of a human-like cerebello-thalamic-basal ganglia pathway capable of modifying vocal motor output.
Assuntos
Gânglios da Base , Cerebelo , Imagem Ecoplanar/métodos , Tentilhões/fisiologia , Atividade Motora/fisiologia , Plasticidade Neuronal/fisiologia , Tálamo , Vocalização Animal/fisiologia , Animais , Gânglios da Base/diagnóstico por imagem , Gânglios da Base/patologia , Gânglios da Base/fisiologia , Cerebelo/diagnóstico por imagem , Cerebelo/patologia , Cerebelo/fisiologia , Imagem de Tensor de Difusão/métodos , Estudos Longitudinais , Masculino , Tálamo/diagnóstico por imagem , Tálamo/patologia , Tálamo/fisiologiaRESUMO
Neurological insults affect both, brain structure and behavior. The injury-induced brain plasticity and associated changes in behavior are difficult to study using classical histological methods. The magnetic resonance imaging (MRI), however, enables repeated inspection of the brain in the same individual. Here we took advantage of the songbird model with discrete brain circuitry controlling song learning and production and assessed if a conventional MRI is suitable to detect even relatively small brain changes. Our aim was to monitor injury and the following regeneration in the striatal vocal nucleus Area X that controls vocal learning in juveniles and affects song in adult songbird zebra finch (Taeniopygia guttata). The regeneration process was detected using T2-weighted images and validated by immunohistochemical (IHC) staining up to 6 months after the injury. Despite the small volume of the zebra finch brain, a satisfactory signal-to-noise ratio was achieved with reasonably short measurement times. No significant difference was found between the measurements of the lesion size obtained by MRI and IHC staining. Our data show that the non-invasive MRI technique can reliably measure and quantify the regeneration process even in a relatively small part of the brain and that the avian striatum progressively regenerates after its neurotoxic injury.
Assuntos
Envelhecimento/patologia , Lesões Encefálicas/diagnóstico por imagem , Lesões Encefálicas/patologia , Corpo Estriado/diagnóstico por imagem , Corpo Estriado/lesões , Regeneração Nervosa/fisiologia , Animais , Lesões Encefálicas/fisiopatologia , Corpo Estriado/patologia , Tentilhões/anatomia & histologia , Tentilhões/lesões , Aumento da Imagem , Imageamento por Ressonância Magnética , Masculino , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
Songbirds, like humans, learn vocalizations and their striatum recruits new neurons in adulthood. Injury in striatal vocal nucleus Area X, involved in song learning and production in songbirds, is followed by massive regeneration. The newborn neurons arise from the subventricular zone (SVZ) rich in dopamine D3 receptors (D3Rs). The aim of this study was to investigate whether the D3Rs affect the rate of neuronal recovery in Area X. Male zebra finches (Taeniopygia guttata) received bilateral neurotoxic lesion of Area X and were implanted with osmotic minipumps containing D3R agonist 7-OH-DPAT, antagonist U99194, or saline. Treatment with 7-OH-DPAT but not U99194 led to significant reduction of lesion size and increased numbers of migrating neuroblasts and newborn cells in the Area X. These cells were detected in the lesion border as well as the lesion center. Lesion also led to increased mRNA expression of the D3Rs in the neurogenic SVZ and in the nucleus robustus arcopallialis (RA) involved in song production. Moreover, lesion alone prolonged the song duration and this may be facilitated by D3Rs in RA. Parallel lesion and stimulation of D3Rs prolonged it even more, while blocking of D3Rs abolished the lesion-induced effect. These data suggest that D3R stimulation after striatal injury accelerates the striatal recovery and can cause behavioral alterations.
Assuntos
Proteínas Aviárias/metabolismo , Corpo Estriado/lesões , Corpo Estriado/metabolismo , Tentilhões/fisiologia , Receptores de Dopamina D3/metabolismo , Vocalização Animal/fisiologia , Animais , Proteínas Aviárias/agonistas , Proteínas Aviárias/antagonistas & inibidores , Movimento Celular/efeitos dos fármacos , Movimento Celular/fisiologia , Corpo Estriado/efeitos dos fármacos , Corpo Estriado/patologia , Modelos Animais de Doenças , Dopaminérgicos/farmacologia , Ácido Ibotênico , Indanos/farmacologia , Masculino , Células-Tronco Neurais/efeitos dos fármacos , Células-Tronco Neurais/metabolismo , Células-Tronco Neurais/patologia , Neurogênese/efeitos dos fármacos , Neurogênese/fisiologia , Plasticidade Neuronal/efeitos dos fármacos , Plasticidade Neuronal/fisiologia , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Neurônios/patologia , RNA Mensageiro/metabolismo , Receptores de Dopamina D3/agonistas , Receptores de Dopamina D3/antagonistas & inibidores , Recuperação de Função Fisiológica/efeitos dos fármacos , Recuperação de Função Fisiológica/fisiologia , Nicho de Células-Tronco/efeitos dos fármacos , Nicho de Células-Tronco/fisiologia , Tetra-Hidronaftalenos/farmacologia , Vocalização Animal/efeitos dos fármacosRESUMO
A pallial-basal-ganglia-thalamic-pallial loop in songbirds is involved in vocal motor learning. Damage to its basal ganglia part, Area X, in adult zebra finches has been noted to have no strong effects on song and its function is unclear. Here we report that neurotoxic damage to adult Area X induced changes in singing tempo and global syllable sequencing in all animals, and considerably increased syllable repetition in birds whose song motifs ended with minor repetitions before lesioning. This stuttering-like behavior started at one month, and improved over six months. Unexpectedly, the lesioned region showed considerable recovery, including immigration of newly generated or repaired neurons that became active during singing. The timing of the recovery and stuttering suggest that immature recovering activity of the circuit might be associated with stuttering. These findings indicate that even after juvenile learning is complete, the adult striatum plays a role in higher level organization of learned vocalizations.
Assuntos
Gânglios da Base/fisiologia , Aves Canoras/fisiologia , Vocalização Animal/fisiologia , Animais , Córtex Cerebral/fisiologia , Aprendizagem/fisiologia , Neurônios/fisiologiaRESUMO
Based on quantitative cluster analyses of 52 constitutively expressed or behaviorally regulated genes in 23 brain regions, we present a global view of telencephalic organization of birds. The patterns of constitutively expressed genes revealed a partial mirror image organization of three major cell populations that wrap above, around, and below the ventricle and adjacent lamina through the mesopallium. The patterns of behaviorally regulated genes revealed functional columns of activation across boundaries of these cell populations, reminiscent of columns through layers of the mammalian cortex. The avian functionally regulated columns were of two types: those above the ventricle and associated mesopallial lamina, formed by our revised dorsal mesopallium, hyperpallium, and intercalated hyperpallium; and those below the ventricle, formed by our revised ventral mesopallium, nidopallium, and intercalated nidopallium. Based on these findings and known connectivity, we propose that the avian pallium has four major cell populations similar to those in mammalian cortex and some parts of the amygdala: 1) a primary sensory input population (intercalated pallium); 2) a secondary intrapallial population (nidopallium/hyperpallium); 3) a tertiary intrapallial population (mesopallium); and 4) a quaternary output population (the arcopallium). Each population contributes portions to columns that control different sensory or motor systems. We suggest that this organization of cell groups forms by expansion of contiguous developmental cell domains that wrap around the lateral ventricle and its extension through the middle of the mesopallium. We believe that the position of the lateral ventricle and its associated mesopallium lamina has resulted in a conceptual barrier to recognizing related cell groups across its border, thereby confounding our understanding of homologies with mammals.
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Aves/anatomia & histologia , Cérebro/anatomia & histologia , Cérebro/metabolismo , Proteínas do Tecido Nervoso/metabolismo , Animais , Contagem de Células , Expressão Gênica , Imageamento Tridimensional , Proteínas do Tecido Nervoso/genética , Neuroimagem , Neurônios/metabolismo , Especificidade da EspécieRESUMO
Song learning and production have many parallels with speech and the mechanisms of their control have been studied extensively. There is an increasing amount of evidence that the dopaminergic system is involved in song learning and maintenance. Dopamine receptors show distinct expression in most of the song nuclei and the highest levels in Area X of the striatum. Here we have investigated whether the mRNA expressions for D1A, D1B, and D2 receptors in Area X are associated with quantitative and/or qualitative characteristics of zebra finch song. We found that quantitative parameters of song such as the amount of songs sang, motif duration, and numbers of distinct syllables and/or notes per motif did not correlate with expression of D1A, D1B nor D2 receptors in Area X or surrounding striatum. However, the mean accuracy of the song correlated negatively with D1A receptor expression levels and the sequential match correlated positively with D2 receptor expression levels in Area X relative to the surrounding striatum. These data suggest that dopamine receptor densities in Area X are associated with song variability.
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Encéfalo/fisiologia , Dopamina/metabolismo , Tentilhões/fisiologia , Aprendizagem/fisiologia , Receptores Dopaminérgicos/metabolismo , Canto , Animais , Masculino , Distribuição TecidualRESUMO
Dopamine is a key neuromodulatory transmitter in the brain. It acts through dopamine receptors to affect changes in neural activity, gene expression, and behavior. In songbirds, dopamine is released into the striatal song nucleus Area X, and the levels depend on social contexts of undirected and directed singing. This differential release is associated with differential expression of activity-dependent genes, such as egr1 (avian zenk), which in mammalian brain are modulated by dopamine receptors. Here we cloned from zebra finch brain cDNAs of all avian dopamine receptors: the D1 (D1A, D1B, D1D) and D2 (D2, D3, D4) families. Comparative sequence analyses of predicted proteins revealed expected phylogenetic relationships, in which the D1 family exists as single exon and the D2 family exists as spliced exon genes. In both zebra finch and chicken, the D1A, D1B, and D2 receptors were highly expressed in the striatum, the D1D and D3 throughout the pallium and within the mesopallium, respectively, and the D4 mainly in the cerebellum. Furthermore, within the zebra finch, all receptors, except for D4, showed differential expression in song nuclei relative to the surrounding regions and developmentally regulated expression that decreased for most receptors during the sensory acquisition and sensorimotor phases of song learning. Within Area X, half of the cells expressed both D1A and D2 receptors, and a higher proportion of the D1A-only-containing neurons expressed egr1 during undirected but not during directed singing. Our findings are consistent with hypotheses that dopamine receptors may be involved in song development and social context-dependent behaviors.
Assuntos
Proteínas Aviárias/metabolismo , Encéfalo/fisiologia , Tentilhões/metabolismo , Neurônios/fisiologia , Receptores de Dopamina D1/metabolismo , Receptores de Dopamina D2/metabolismo , Animais , Proteínas Aviárias/genética , Galinhas/genética , Galinhas/metabolismo , Feminino , Tentilhões/genética , Masculino , Receptores de Dopamina D1/genética , Receptores de Dopamina D2/genética , Receptores de Dopamina D3/genética , Receptores de Dopamina D3/metabolismo , Receptores de Dopamina D4/genética , Receptores de Dopamina D4/metabolismo , Receptores de Dopamina D5/genética , Receptores de Dopamina D5/metabolismo , Homologia de Sequência , Comportamento Social , Especificidade da Espécie , Vocalização Animal/fisiologiaRESUMO
Dopamine function in birdsong has been studied extensively in recent years. Several song and auditory nuclei are innervated by midbrain dopaminergic fibers and contain neurons with various dopamine receptors. During sexually motivated singing, activity of midbrain dopaminergic neurons in the ventral tegmental area and dopamine release in the striatal Area X, involved in song learning and maintenance, are higher. In this review we provide an overview of the dopaminergic system and neurotransmission in songbirds and the outline of possible involvement of dopamine in control of song learning, production, and maintenance. Based on both behavioral and computational biology data, we describe several models of song learning and the proposed role of dopamine in them. Special attention is given to possible role of dopamine in incentive salience (wanting) and reward prediction error signaling during song learning and maintenance, as well as the role of dopamine-mediated synaptic plasticity in reward processing. Finally, the role of dopamine in determination of personality traits in relation to birdsong is discussed.
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Dopamina/fisiologia , Aprendizagem/fisiologia , Vocalização Animal/fisiologia , Animais , Encéfalo/anatomia & histologia , Encéfalo/fisiologia , Vias Neurais/anatomia & histologia , Vias Neurais/fisiologia , Plasticidade Neuronal/fisiologia , Reforço Psicológico , RecompensaRESUMO
Although the avian brain dopamine system and its functions have been studied much less than the mammalian one, there is an increasing interest in the role of dopamine and its receptors in a wide variety of motor, cognitive and emotional functions in birds with implications for basic research, medicine or agriculture. Pharmacological characterisation of the avian dopamine receptors has had little attention. In this paper we characterise the two classes of dopamine receptors in Japanese quail brain by radioligand binding techniques using [(3)H]SCH 23390 (D(1)) and [(3)H]spiperone (D(2)). Association, dissociation and saturation analyses showed that the binding of both radioligands is time- and concentration-dependent, saturable and reversible. Apparent dissociation constants determined for [(3)H]SCH 23390 and [(3)H]spiperone from concentration isotherms were 1.07 and 0.302 nM and the maximum binding capacities were 89.3 and 389.3 fmol per mg of protein, respectively. Using competitive binding studies with a spectrum of dopamine and other neurotransmitter receptor agonists/antagonists, the [(3)H]SCH 23390 and [(3)H]spiperone binding sites were characterised pharmacologically. Pharmacological profiles of quail dopamine receptors showed a high degree of pharmacological homology with other vertebrate dopamine receptors. The data presented extend the knowledge of kinetics and pharmacology of D(1)- and D(2)-like dopamine receptors in birds, provide data for avian psychopharmacological and comparative studies and represent an important complement to studies using cell expression systems.
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Agonistas de Dopamina/farmacologia , Agonistas de Dopamina/farmacocinética , Antagonistas de Dopamina/farmacologia , Antagonistas de Dopamina/farmacocinética , Receptores de Dopamina D1/metabolismo , Receptores de Dopamina D2/metabolismo , Animais , Coturnix , Prosencéfalo/efeitos dos fármacos , Prosencéfalo/metabolismo , Ensaio RadioliganteRESUMO
Social context has been shown to have a profound influence on brain activation in a wide range of vertebrate species. Best studied in songbirds, when males sing undirected song, the level of neural activity and expression of immediate early genes (IEGs) in several song nuclei is dramatically higher or lower than when they sing directed song to other birds, particularly females. This differential social context-dependent activation is independent of auditory input and is not simply dependent on the motor act of singing. These findings suggested that the critical sensory modality driving social context-dependent differences in the brain could be visual cues. Here, we tested this hypothesis by examining IEG activation in song nuclei in hemispheres to which visual input was normal or blocked. We found that covering one eye blocked visually induced IEG expression throughout both contralateral visual pathways of the brain, and reduced activation of the contralateral ventral tegmental area, a non-visual midbrain motivation-related area affected by social context. However, blocking visual input had no effect on the social context-dependent activation of the contralateral song nuclei during female-directed singing. Our findings suggest that individual sensory modalities are not direct driving forces for the social context differences in song nuclei during singing. Rather, these social context differences in brain activation appear to depend more on the general sense that another individual is present.
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Tentilhões/fisiologia , Comportamento Social , Percepção Visual , Vocalização Animal/fisiologia , Animais , Encéfalo/anatomia & histologia , Encéfalo/fisiologia , Mapeamento Encefálico , Cognição , Eletrofisiologia , Feminino , Tentilhões/anatomia & histologia , Genes Precoces/fisiologia , Masculino , Estimulação LuminosaRESUMO
In a well-studied model of social behaviour, male zebra finches sing directed song to court females and undirected song, used possibly for practice or advertisement. Although the two song types are similar, the level of neural activity and expression of the immediate early gene egr-1 are higher during undirected than during directed singing in the lateral part of the basal ganglia song nucleus AreaX (LAreaX) and its efferent pallial song nuclei lateral magnocellular nucleus of the anterior nidopallium (LMAN) and the robust nucleus of the arcopallium (RA). As social interactions are dependent on brain motivation systems, here we test the hypothesis that the midbrain ventral tegmental area-substantia nigra pars compacta (VTA-SNc) complex, which provides a strong dopaminergic input to LAreaX, is a source of this modulation. Using egr-1 expression, we show that GABAergic interneurons in VTA-SNc are more active during directed courtship singing than during undirected singing. We also found that unilateral removal of VTA-SNc input reduced singing-dependent gene expression in ipsilateral LAreaX during both social contexts but it did not eliminate social context differences in LAreaX. In contrast, such lesions reduced and eliminated the social context differences in efferent nuclei LMAN and RA, respectively. These results suggest that VTA-SNc is not solely responsible for the social context gene regulation in LAreaX, but that VTA-SNc input to LAreaX enhances the singing-regulated gene expression in this nucleus and, either through LAreaX or through direct projections to LMAN and RA, VTA-SNc is necessary for context-dependent gene regulation in these efferent nuclei.
Assuntos
Dopamina/metabolismo , Centro Vocal Superior/fisiologia , Mesencéfalo/fisiologia , Comportamento Social , Vocalização Animal , Adrenérgicos/farmacologia , Animais , Comportamento Animal , Proteína 1 de Resposta de Crescimento Precoce/genética , Proteína 1 de Resposta de Crescimento Precoce/metabolismo , Tentilhões , Lateralidade Funcional , Regulação da Expressão Gênica/fisiologia , Centro Vocal Superior/citologia , Centro Vocal Superior/efeitos dos fármacos , Centro Vocal Superior/lesões , Imuno-Histoquímica/métodos , Hibridização In Situ/métodos , Masculino , Mesencéfalo/efeitos dos fármacos , Vias Neurais/fisiologia , Neurônios/metabolismo , Oxidopamina/farmacologia , Tirosina 3-Mono-Oxigenase/metabolismo , Vocalização Animal/efeitos dos fármacos , Ácido gama-Aminobutírico/metabolismoRESUMO
The discrete neural network for songbird vocal communication provides an effective system to study neural mechanisms of learned motor behaviors in vertebrates. This system consists of two pathways--a vocal motor pathway used to produce learned vocalizations and a vocal pallial basal ganglia loop used to learn and modify the vocalizations. However, it is not clear how the loop exerts control over the motor pathway. To study the mechanism, we used expression of the neural activity-induced gene ZENK (or egr-1), which shows singing-regulated expression in a social context-dependent manner: high levels in both pathways when singing undirected and low levels in the lateral part of the loop and in the robust nucleus of the arcopallium (RA) of the motor pathway when singing directed to another animal. Here, we show that there are two parallel interactive parts within the pallial basal ganglia loop, lateral and medial, which modulate singing-driven ZENK expression of the motor pathway nuclei RA and HVC, respectively. Within the loop, the striatal and pallial nuclei appear to have opposing roles; the striatal vocal nucleus lateral AreaX is required for high ZENK expression in its downstream nuclei, particularly during undirected singing, while the pallial vocal lateral magnocellular nucleus of the anterior nidopallium is required for lower expression, particularly during directed singing. These results suggest a dynamic molecular interaction between the basal ganglia pathway and the motor pathway during production of a learned motor behavior.
Assuntos
Gânglios da Base/anatomia & histologia , Vias Eferentes/fisiologia , Tentilhões , Regulação da Expressão Gênica , Vocalização Animal/fisiologia , Animais , Gânglios da Base/patologia , Gânglios da Base/fisiologia , Proteína 1 de Resposta de Crescimento Precoce/genética , Proteína 1 de Resposta de Crescimento Precoce/metabolismo , Vias Eferentes/anatomia & histologia , Vias Eferentes/patologia , Corantes Fluorescentes/metabolismo , Masculino , Análise de RegressãoRESUMO
We believe that names have a powerful influence on the experiments we do and the way in which we think. For this reason, and in the light of new evidence about the function and evolution of the vertebrate brain, an international consortium of neuroscientists has reconsidered the traditional, 100-year-old terminology that is used to describe the avian cerebrum. Our current understanding of the avian brain - in particular the neocortex-like cognitive functions of the avian pallium - requires a new terminology that better reflects these functions and the homologies between avian and mammalian brains.
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Evolução Biológica , Encéfalo/fisiologia , Animais , Aves , Humanos , VertebradosRESUMO
When pigeons are repeatedly administered a dose of apomorphine they show an increasing behavioral response, much as rodents do. In birds this expresses itself in an augmented pecking response. This sensitization is assumed to be largely due to a conditioning process. Here we present evidence that sensitization is accompanied by an alteration of the D(1) to D(2) dopamine receptor densities. An experimental group of pigeons was repeatedly injected with apomorphine, and a control group with saline. The basal forebrain tissue, known to be rich in dopamine receptors, was subjected to binding assays using tritiated specific D(1) and D(2) dopamine receptor antagonists. There was a trend towards an increase in D(1) and a significant decrease in D(2) receptor densities in apomorphine-treated birds compared to the saline-treated controls. We conclude that extended apomorphine treatment modifies the D(1) dopamine receptor density in the opposite manner to the D(2) dopamine receptor density.
Assuntos
Apomorfina/farmacologia , Columbidae/fisiologia , Receptores de Dopamina D1/agonistas , Receptores de Dopamina D2/agonistas , Telencéfalo/efeitos dos fármacos , Animais , Ligação Competitiva/efeitos dos fármacos , Ligação Competitiva/fisiologia , Columbidae/anatomia & histologia , Condicionamento Psicológico/efeitos dos fármacos , Condicionamento Psicológico/fisiologia , Corpo Estriado/efeitos dos fármacos , Corpo Estriado/metabolismo , Dopamina/metabolismo , Agonistas de Dopamina/farmacologia , Antagonistas de Dopamina/farmacologia , Atividade Motora/efeitos dos fármacos , Atividade Motora/fisiologia , Vias Neurais/efeitos dos fármacos , Vias Neurais/metabolismo , Plasticidade Neuronal/efeitos dos fármacos , Plasticidade Neuronal/fisiologia , Ensaio Radioligante , Agregação de Receptores/efeitos dos fármacos , Agregação de Receptores/fisiologia , Receptores de Dopamina D1/metabolismo , Receptores de Dopamina D2/metabolismo , Transmissão Sináptica/efeitos dos fármacos , Transmissão Sináptica/fisiologia , Telencéfalo/metabolismoRESUMO
The standard nomenclature that has been used for many telencephalic and related brainstem structures in birds is based on flawed assumptions of homology to mammals. In particular, the outdated terminology implies that most of the avian telencephalon is a hypertrophied basal ganglia, when it is now clear that most of the avian telencephalon is neurochemically, hodologically, and functionally comparable to the mammalian neocortex, claustrum, and pallial amygdala (all of which derive from the pallial sector of the developing telencephalon). Recognizing that this promotes misunderstanding of the functional organization of avian brains and their evolutionary relationship to mammalian brains, avian brain specialists began discussions to rectify this problem, culminating in the Avian Brain Nomenclature Forum held at Duke University in July 2002, which approved a new terminology for avian telencephalon and some allied brainstem cell groups. Details of this new terminology are presented here, as is a rationale for each name change and evidence for any homologies implied by the new names. Revisions for the brainstem focused on vocal control, catecholaminergic, cholinergic, and basal ganglia-related nuclei. For example, the Forum recognized that the hypoglossal nucleus had been incorrectly identified as the nucleus intermedius in the Karten and Hodos (1967) pigeon brain atlas, and what was identified as the hypoglossal nucleus in that atlas should instead be called the supraspinal nucleus. The locus ceruleus of this and other avian atlases was noted to consist of a caudal noradrenergic part homologous to the mammalian locus coeruleus and a rostral region corresponding to the mammalian A8 dopaminergic cell group. The midbrain dopaminergic cell group in birds known as the nucleus tegmenti pedunculopontinus pars compacta was recognized as homologous to the mammalian substantia nigra pars compacta and was renamed accordingly; a group of gamma-aminobutyric acid (GABA)ergic neurons at the lateral edge of this region was identified as homologous to the mammalian substantia nigra pars reticulata and was also renamed accordingly. A field of cholinergic neurons in the rostral avian hindbrain was named the nucleus pedunculopontinus tegmenti, whereas the anterior nucleus of the ansa lenticularis in the avian diencephalon was renamed the subthalamic nucleus, both for their evident mammalian homologues. For the basal (i.e., subpallial) telencephalon, the actual parts of the basal ganglia were given names reflecting their now evident homologues. For example, the lobus parolfactorius and paleostriatum augmentatum were acknowledged to make up the dorsal subdivision of the striatal part of the basal ganglia and were renamed as the medial and lateral striatum. The paleostriatum primitivum was recognized as homologous to the mammalian globus pallidus and renamed as such. Additionally, the rostroventral part of what was called the lobus parolfactorius was acknowledged as comparable to the mammalian nucleus accumbens, which, together with the olfactory tubercle, was noted to be part of the ventral striatum in birds. A ventral pallidum, a basal cholinergic cell group, and medial and lateral bed nuclei of the stria terminalis were also recognized. The dorsal (i.e., pallial) telencephalic regions that had been erroneously named to reflect presumed homology to striatal parts of mammalian basal ganglia were renamed as part of the pallium, using prefixes that retain most established abbreviations, to maintain continuity with the outdated nomenclature. We concluded, however, that one-to-one (i.e., discrete) homologies with mammals are still uncertain for most of the telencephalic pallium in birds and thus the new pallial terminology is largely devoid of assumptions of one-to-one homologies with mammals. The sectors of the hyperstriatum composing the Wulst (i.e., the hyperstriatum accessorium intermedium, and dorsale), the hyperstriatum ventrale, the neostriatum, and the archistriatum have been renamed (respectively) the hyperpallium (hypertrophied pallium), the mesopallium (middle pallium), the nidopallium (nest pallium), and the arcopallium (arched pallium). The posterior part of the archistriatum has been renamed the posterior pallial amygdala, the nucleus taeniae recognized as part of the avian amygdala, and a region inferior to the posterior paleostriatum primitivum included as a subpallial part of the avian amygdala. The names of some of the laminae and fiber tracts were also changed to reflect current understanding of the location of pallial and subpallial sectors of the avian telencephalon. Notably, the lamina medularis dorsalis has been renamed the pallial-subpallial lamina. We urge all to use this new terminology, because we believe it will promote better communication among neuroscientists. Further information is available at http://avianbrain.org