Degradation of SDS by psychrotolerant Staphylococcus saprophyticus and Bacillus pumilus isolated from Southern Ocean water samples.

Bioremediation of surfactants in water bodies holds significant ecological importance as they are contaminants of emerging concern posing substantial threats to the aquatic environment. Microbes exhibiting special ability in terms of bioremediation of contaminants have always been reported to thrive in extraordinary environmental conditions that can be extreme in terms of temperature, lack of nutrients, and salinity. Therefore, in the present investigation, a total of 46 bacterial isolates were isolated from the Indian sector of the Southern Ocean and screened for degradation of sodium dodecyl sulphate (SDS). Further, two Gram-positive psychrotolerant bacterial strains, ASOI-01 and ASOI-02 were identified with significant SDS degradation potential. These isolates were further studied for growth optimization under different environmental conditions. The strains were characterized as Staphylococcus saprophyticus and Bacillus pumilus based on morphological, biochemical, and molecular (16S RNA gene) characteristics. The study reports 88.9% and 93.4% degradation of SDS at a concentration of 100 mgL-1, at 20 °C, and pH 7 by S. saprophyticus ASOI-01 and B. pumilus ASOI-02, respectively. The experiments were also conducted in wastewater samples where a slight reduction in degradation efficiency was observed with strains ASOI-01 and ASOI-02 exhibiting 76.83 and 64.93% degradation of SDS respectively. This study infers that these bacteria can be used for the bioremediation of anionic surfactants from water bodies and establishes the potential of extremophilic microbes for the utilization of sustainable wastewater management.

Speciation of macro- and nanoparticles of Cr2O3 in Hordeum vulgare L. and subsequent toxicity: A comparative study.

Chromium (Cr) is reported to be hazardous to environmental components and surrounding biota when levels exceed allowable thresholds. As Cr is extensively utilized in different industries, thereby comprehensively studied for its toxicity. Along with Cr, the applications of nano-Cr or chromium oxide nanoparticles (Cr2O3-NPs) are also expanding; however, the literature is scarce or limited on their phytotoxicity. Thereby, the current work investigated the morpho-physiological insights of macro- and nanoparticles of Cr in Hordeum vulgare L. plants. The increased accumulation and translocation of Cr under the exposure of both forms disturbed the cellular metabolism that might have inhibited germination and growth as well as interfered with the photosynthesis of plants. The overall extent of toxicity was noticeably higher under nanoparticles' exposure than macroparticles of Cr. The potential cue for such phytotoxic consequences mediated by Cr nanoparticles could be an increased bioavailability of Cr ions which was also supported by their total content, mobility, and factor toxicity index. Besides, to support further these findings, synchrotron X-ray technique was used to reliably identify Cr-containing compounds in the plant tissues. The X-ray spectra of the near spectral region and the far region of the spectrum of K-edge of Cr were obtained, and it was established that the dominant crystalline phase corresponds to Cr2O3 (eskolaite) from the recorded observations. Thus, the obtained results would allow revealing the mechanism of macro- and nanoparticles of Cr induced impacts on plant at the tissue, cellular- and sub-cellular levels.

Morpho-biochemical Responses and Disturbed Redox Homeostasis in Barley Under Benzyl-butyl Phthalate Stress.

The present study is aimed to address the morphometric consequences, yield attributes, and biochemical responses of barley plants under the stress of an endocrine disruptor i.e., benzyl-butyl phthalate (BBP). The morphometric analyses (plant length, dry weight, and net primary productivity) revealed that the inhibition induced by BBP was concentration- and time-dependent. The seed weight and the number of seeds per spike have also significantly declined with an increase in BBP doses. Similarly, BBP exhibited significant alterations over the control in the biochemical indices viz., pigments, sugars, proteins, proline, malonaldehyde, and hydrogen peroxide contents of barley plants. Furthermore, BBP stress negatively influenced the activities of antioxidative enzymes viz., SOD, POD, CAT, APX, and GR of barley with an increase in doses and exposure durations due to the over-produced reactive oxygen species. The uptake and transport of BBP were determined and observed as a responsible cue for these toxicological implications in barley plants under BBP exposure. The correlation of barley plants' morpho-biochemical responses with BBP uptake and transport was also established using Pearson's correlation. Thus, this study indicated the toxicological behavior of meagerly explored phthalate (i.e., BBP) in the crop plant and these observations can be utilized for the generation of tolerant cultivars.

Genotoxic and morpho-physiological responses of ZnO macro- and nano-forms in plants.

In the current study, two plants, viz., Pisum sativum L. and Hordeum vulgare L., were exposed to nano- and macro-dispersed ZnO at 1, 10, and 30 times of maximal permissible concentration (MPC). The main objective of the study is to depict and compare the genotoxicity in terms of chromosomal anomalies, cytotoxicity (i.e., mitotic index), and phytotoxicity (viz., germination, morphometry, maximal quantum yield, and chlorophyll fluorescence imaging) of macro- and nano-forms of ZnO along with their accumulation and translocation. In the case of genotoxic and cytotoxic responses, the maximal effect was observed at 30 MPC, regardless of the macro- or nano-forms of ZnO. The phytotoxic observations revealed that the treatment with macro- and nano-forms of ZnO significantly affected the germination rate, germination energy, and length of roots and shoots of H. vulgare in a dose-dependent manner. The factor toxicity index of treated soil demonstrated that toxicity soared as concentrations increased and that at 30 MPC, toxicity was average and high in macro- and nano-dispersed ZnO, respectively. Furthermore, the photosynthetic parameters were observed to be negatively affected in both treatments, but the maximal effect was observed in the case of nano-dispersed form. It was noted that the mobility of nano-dispersed ZnO in the soil was higher than macro-dispersed. The increased mobility of nano-dispersed ZnO might have boosted their accumulation and translocation that subsequently led to the oxidative stress due to the accelerated production of reactive oxygen species, thus strengthen toxicity implications in plants.

A practical evaluation on integrated role of biochar and nanomaterials in soil remediation processes.

Soil decontamination and restoration continue to be a key environmental concern around the globe. The degradation of soil resources due to the presence of potentially toxic elements (PTEs) has a substantial influence on agricultural production, food security, and human well-being, and as a result, urgent action is required. PTEs pollution is not a threat to the agroecosystems but also a serious concern to human health; thereby, it needs to be addressed timely and effectively. Hence, the development of improved and cost-effective procedures to remove PTEs from polluted soils is imperative. With this context in mind, current review is designed to distinctly envisage the PTEs removal potential by the single and binary applications of biochar (BC) and nanomaterials (NMs).2 Recently, BC, a product of high-temperature biomass pyrolysis with high specific surface area, porosity, and distinctive physical and chemical properties has become one of the most used and economic adsorbent materials. Also, biochar's application has generated interest in a variety of fields and environments as a modern approach against the era of urbanization, industrialization, and climate change. Likewise, several NMs including metals and their oxides, carbon materials, zeolites, and bimetallic-based NMs have been documented as having the potential to remediate PTEs-polluted environments. However, both techniques have their own set of advantages and disadvantages, therefore combining them can be a more effective strategy to address the growing concern over the rapid accumulation and release of PTEs into the environment.

Plant growth-promoting rhizobacteria: a potential bio-asset for restoration of degraded soil and crop productivity with sustainable emerging techniques.

The rapid expansion of degraded soil puts pressure on agricultural crop yield while also increasing the likelihood of food scarcity in the near future at the global level. The degraded soil does not suit plants growth owing to the alteration in biogeochemical cycles of nutrients, soil microbial diversity, soil organic matter, and increasing concentration of heavy metals and organic chemicals. Therefore, it is imperative that a solution should be found for such emerging issues in order to establish a sustainable future. In this context, the importance of plant growth-promoting rhizobacteria (PGPR) for their ability to reduce plant stress has been recognized. A direct and indirect mechanism in plant growth promotion is facilitated by PGPR via phytostimulation, biofertilizers, and biocontrol activities. However, plant stress mediated by deteriorated soil at the field level is not entirely addressed by the implementation of PGPR at the field level. Thus, emerging methods such as CRISPR and nanotechnological approaches along with PGPR could manage degraded soil effectively. In the pursuit of the critical gaps in this respect, the present review discusses the recent advancement in PGPR action when used along with nanomaterials and CRISPR, impacting plant growth under degraded soil, thereby opening a new horizon for researchers in this field to mitigate the challenges of degraded soil.

Advances in characterization of probiotics and challenges in industrial application.

An unbalanced diet and poor lifestyle are common reasons for numerous health complications in humans. Probiotics are known to provide substantial benefits to human health by producing several bioactive compounds, vitamins, short-chain fatty acids and short peptides. Diets that contain probiotics are limited to curd, yoghurt, kefir, kimchi, etc. However, exploring the identification of more potential probiotics and enhancing their commercial application to improve the nutritional quality would be a significant step to utilizing the maximum benefits. The complex evolution patterns among the probiotics are the hurdles in their characterization and adequate application in the industries and dairy products. This article has mainly discussed the molecular methods of characterization that are based on the analysis of ribosomal RNA, whole genome, and protein markers and profiles. It also has critically emphasized the emerging challenges in industrial applications of probiotics.

Nanobionics in Crop Production: An Emerging Approach to Modulate Plant Functionalities.

The "Zero Hunger" goal is one of the key Sustainable Development Goals (SDGs) of the United Nations. Therefore, improvements in crop production have always been a prime objective to meet the demands of an ever-growing population. In the last decade, studies have acknowledged the role of photosynthesis augmentation and enhancing nutrient use efficiency (NUE) in improving crop production. Recently, the applications of nanobionics in crop production have given hope with their lucrative properties to interact with the biological system. Nanobionics have significantly been effective in modulating the photosynthesis capacity of plants. It is documented that nanobionics could assist plants by acting as an artificial photosynthetic system to improve photosynthetic capacity, electron transfer in the photosystems, and pigment content, and enhance the absorption of light across the UV-visible spectrum. Smart nanocarriers, such as nanobionics, are capable of delivering the active ingredient nanocarrier upon receiving external stimuli. This can markedly improve NUE, reduce wastage, and improve cost effectiveness. Thus, this review emphasizes the application of nanobionics for improving crop yield by the two above-mentioned approaches. Major concerns and future prospects associated with the use of nanobionics are also deliberated concisely.

Nanotechnology in the Restoration of Polluted Soil.

The advancements in nanoparticles (NPs) may be lighting the sustainable and eco-friendly path to accelerate the removal of toxic compounds from contaminated soils. Many efforts have been made to increase the efficiency of phytoremediation, such as the inclusion of chemical additives, the application of rhizobacteria, genetic engineering, etc. In this context, the integration of nanotechnology with bioremediation has introduced new dimensions for revamping the remediation methods. Hence, advanced remediation approaches combine nanotechnological and biological remediation methods in which the nanoscale process regulation supports the adsorption and deterioration of pollutants. Nanoparticles absorb/adsorb a large variety of contaminants and also catalyze reactions by lowering the energy required to break them down, owing to their unique surface properties. As a result, this remediation process reduces the accumulation of pollutants while limiting their spread from one medium to another. Therefore, this review article deals with all possibilities for the application of NPs for the remediation of contaminated soils and associated environmental concerns.

Uptake of a plasticizer (di-n-butyl phthalate) impacts the biochemical and physiological responses of barley.

BACKGROUND: DBP is one of the most commonly used plasticizers for imparting desirable properties to polymers. The introduction of phthalates is reported to have occurred in the late 1920s, and there has been a significant rise in their release into the environment in past decades due to a lack of covalent bonding with the parent matrix. Because of their numerous applications in day-to-day life, phthalates have become ubiquitous and also classified as endocrine disruptors. Hence, several studies have been conducted to investigate the phthalate-mediated toxicities in animals; however, plants have not been explored to the same amount. METHODS: Therefore, in the present study, the accumulation and translocation along with morpho-physiological perturbations in barley plants after 15, 30, 60, and 120 days of exposure to di-n-butyl phthalate (DBP) are investigated using standard protocols. RESULTS: The maximal accumulation and translocation of DBP in the roots and shoots of barley plants was observed after 60 days of exposure. The exposure of DBP from 15 to 120 days was recorded to decline all the morphological indices (i.e., dry weight, net primary productivity, seed number per spike, and seed weight) of barley plants. The pigments content declined under DBP treatment for all exposure durations except 120 days exposure. Carbohydrate content increased after 15-30 days of exposure afterward it was observed to be decreased under 60 and 120 days of exposure. The protein content was declined in DBP stressed plants for 15-120 days. Proline content was increased in all exposure durations and maximal percent increase was recorded in 120 days of exposure. MDA content showed an increase at earlier exposure durations then followed by a decline in long-term exposure. Hydrogen peroxide content increased at all exposure durations. There were significant alterations observed in the activities of all antioxidative enzymes in comparison to the control. Furthermore, DBP stressed plants after 60 days were analyzed for the macromolecular variations using Fourier transform infrared spectroscopy (FTIR). CONCLUSION: Thus, the outcomes of the current work provide an appraisal of phthalates' uptake and translocation mediated phytotoxic responses in barley plants. These observations can help in developing genetically modified edible plants that are resistant to phthalates uptake, thereby ensuring food security.

Microplastic Pollution: An Emerging Threat to Terrestrial Plants and Insights into Its Remediation Strategies.

Microplastics (MPs) are ubiquitous and constitute a global hazard to the environment because of their robustness, resilience, and long-term presence in the ecosystem. For now, the majority of research has primarily focused on marine and freshwater ecosystems, with just a small amount of attention towards the terrestrial ecosystems. Although terrestrial ecosystems are recognized as the origins and routes for MPs to reach the sea, there is a paucity of knowledge about these ecological compartments, which is necessary for conducting effective ecological risk assessments. Moreover, because of their high persistence and widespread usage in agriculture, agribusiness, and allied sectors, the presence of MPs in arable soils is undoubtedly an undeniable and severe concern. Consequently, in the recent decade, the potential risk of MPs in food production, as well as their impact on plant growth and development, has received a great deal of interest. Thus, a thorough understanding of the fate and risks MPs, as well as prospective removal procedures for safe and viable agricultural operations in real-world circumstances, are urgently needed. Therefore, the current review is proposed to highlight the potential sources and interactions of MPs with agroecosystems and plants, along with their remediation strategies.

Metal(loid) nanosorbents in restoration of polluted soils: geochemical, ecotoxicological, and remediation perspectives.

Nowadays, the applications of nanomaterials (NMs) are becoming the edge over others and referred as one of the pillars of emerging science and technology. Thereby, a wide array of NMs have been developed along with the products that can be used for the reclamation of contaminated terrestrial ecosystems. The NMs got a great consideration due to their peculiar characteristics and high efficacy. Therefore, this review addresses in depth the ability of metal(loid) NMs as nanosorbents along with their applications in soil remediation. Adsorption is commonly employed for the elimination of innumerable contaminants because of low expenses, reliability, and convenience. The first emphasis of this work will be the use of nanoscale meta(loid) adsorbents for contaminated soil remediation along with their geochemistry. Because NMs mediated soil remediation promises more efficient and cost-effective than conventional methods and can enhance the probability of in situ contaminants remediation. However, the extensive usage of NMs is enhancing their concentrations in the environment and get a route to enter the surrounding flora and fauna that can induce serious concerns due to the lack of absolute understanding regarding NMs interactions with living organisms. Therefore, the second focus of this work will be on the ecotoxicological impacts with special attentions on morpho-physiological alterations in edible plants.

Effects of Silicon and Silicon-Based Nanoparticles on Rhizosphere Microbiome, Plant Stress and Growth.

Silicon (Si) is considered a non-essential element similar to cadmium, arsenic, lead, etc., for plants, yet Si is beneficial to plant growth, so it is also referred to as a quasi-essential element (similar to aluminum, cobalt, sodium and selenium). An element is considered quasi-essential if it is not required by plants but its absence results in significant negative consequences or anomalies in plant growth, reproduction and development. Si is reported to reduce the negative impacts of different stresses in plants. The significant accumulation of Si on the plant tissue surface is primarily responsible for these positive influences in plants, such as increasing antioxidant activity while reducing soil pollutant absorption. Because of these advantageous properties, the application of Si-based nanoparticles (Si-NPs) in agricultural and food production has received a great deal of interest. Furthermore, conventional Si fertilizers are reported to have low bioavailability; therefore, the development and implementation of nano-Si fertilizers with high bioavailability could be crucial for viable agricultural production. Thus, in this context, the objectives of this review are to summarize the effects of both Si and Si-NPs on soil microbes, soil properties, plant growth and various plant pathogens and diseases. Si-NPs and Si are reported to change the microbial colonies and biomass, could influence rhizospheric microbes and biomass content and are able to improve soil fertility.

Coping with the Challenges of Abiotic Stress in Plants: New Dimensions in the Field Application of Nanoparticles.

Abiotic stress in plants is a crucial issue worldwide, especially heavy-metal contaminants, salinity, and drought. These stresses may raise a lot of issues such as the generation of reactive oxygen species, membrane damage, loss of photosynthetic efficiency, etc. that could alter crop growth and developments by affecting biochemical, physiological, and molecular processes, causing a significant loss in productivity. To overcome the impact of these abiotic stressors, many strategies could be considered to support plant growth including the use of nanoparticles (NPs). However, the majority of studies have focused on understanding the toxicity of NPs on aquatic flora and fauna, and relatively less attention has been paid to the topic of the beneficial role of NPs in plants stress response, growth, and development. More scientific attention is required to understand the behavior of NPs on crops under these stress conditions. Therefore, the present work aims to comprehensively review the beneficial roles of NPs in plants under different abiotic stresses, especially heavy metals, salinity, and drought. This review provides deep insights about mechanisms of abiotic stress alleviation in plants under NP application.

Accumulation, morpho-physiological and oxidative stress induction by single and binary treatments of fluoride and low molecular weight phthalates in Spirodela polyrhiza L. Schleiden.

The present study examined the interactive effects of fluoride and phthalates on their uptake, generation of reactive oxygen species and activation of antioxidative defence responses in Spirodela polyrhiza L. Schleiden. A hydroponic study was conducted in which S. polyrhiza cultured in Hoagland's nutrient medium, was exposed to fluoride (50 ppm) and different concentrations viz., 75, 150 300 ppm of diethyl phthalate (DEP) and diallyl phthalate (DAP) individually as well as in combination for the time period of 24, 72, 120 and 168 h respectively. A significant decline in fresh weight, dry to fresh weight ratio, total chlorophyll, carotenoid content and increased anthocyanin content was observed. Fluoride and phthalates was found to be readily accumulated by S. polyrhiza in all the exposure periods. Interestingly, when binary treatments were given in nutrient medium, uptake of both fluoride and phthalate was found to be influenced by each other. In combined treatments, DEP stimulated fluoride uptake, while its own uptake was restricted by fluoride. In contrary to this, fluoride stimulated DAP uptake. Moreover, combined stress further caused significant decrement in carbohydrate, protein content and increment in MDA levels, phenolic content and electrolyte leakage. Nevertheless, phthalates showed more pronounced oxidative stress and growth inhibition compared to fluoride. To cope up with the oxidative damage, enhanced level of antioxidant enzymatic activities was observed in S. polyrhiza under both fluoride and phthalate stress as compared to control. Scanning electron microscope imaging of leaf stomata revealed that combined stress of fluoride with phthalates caused distortion in the shape of guard cells. Confocal micrographs confirmed the generation of reactive oxygen species, cell damage, disruption in membrane integrity, and enhanced levels of glutathione in plant cells. This study focussed on ecotoxicological and interactive significance of fluoride led phthalate uptake or vice versa which was also assumed to confer tolerance attributes.

Modulation of biochemical and physiological parameters in Hordeum vulgare L. seedlings under the influence of benzyl-butyl phthalate.

BACKGROUND: Phthalates are man-made chemical compounds with numerous applications especially known for their use as plasticizers. They have weak bonding to the polymeric matrix or products in which they are used. Owing to this reason, they are readily released into the environment which makes them ubiquitous. The agricultural soils are also reported to be polluted with phthalates up to a considerable extent which causes adverse effects on flora and fauna. A few studies have been conducted on phthalate-induced phytotoxicity, which has revealed that phthalates affect the quality and yield of edible plants. In the last decades, some crops were analyzed for phthalate-induced adversities; among them, barley was the least explored. METHODS: The present study has investigated the impact of benzyl-butyl phthalate (BBP) on barley (Hordeum vulgare L.) seedlings to address the biochemical, physiological consequences, and toxicological implications. After the exogenous exposure of BBP (viz. 0, 25, 50, 100, 200, 400, 800, 1,600 mg/L) for 7 days, barley seedlings were analyzed for different indices. RESULTS: The exposure of BBP mediated a significant (p ≤ 0.05, 0.01) overall elevation in the contents of pigment, proline, soluble protein, carbohydrate, hydrogen peroxide (H2O2), and malondialdehyde (MDA) in shoots and roots of barley seedlings. The activities of superoxide dismutase (SOD), guaiacol peroxidase (POD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) were also stimulated significantly in shoots and roots of seedlings against BBP stress except for SOD activity which declined in the roots. The polyphenols (non-enzymatic antioxidants) content was also altered in all the treated concentrations as compared to the control. Furthermore, BBP caused stomatal abnormalities, induced cytotoxicity, and loss of plasma membrane integrity. CONCLUSIONS: BBP disturbed the normal physiology of barley which could also affect the yield of the crop under field conditions.

Multi-residue pesticides analysis in water samples using reverse phase high performance liquid chromatography (RP-HPLC).

India is one of the leading suppliers of agrochemicals and has the largest pesticide industry in Asia. Among various Indian states, Punjab is the primary user of pesticides. Presence of pesticide residue in water and food products of Punjab is well documented. The present study was designed to envisage the level of pesticide contamination in pond water of eleven villages of Amritsar district of Punjab, India. A rapid and concurrent method for the identification and quantification of pesticides in water samples was developed and validated. The method validation parameters exhibited high sensitivity of the developed method and the proficiency for the identification and quantification of pesticide residues in water samples. The RP-HPLC method described here •is a novel method which is applicable for simple, rapid and precise detection of pesticides.•40.02% of water samples were found contaminated with multi-residue pesticides.•carbofuran was the most abundant pesticide which was present in 18.18% samples.