Isolation and characterization of anaerobic bacteria with fiber degradation potential from faeces of Boselaphus tragocamelus grazing on semi arid Indian conditions.

Hindgut of wild ruminants harbours diversified anaerobic bacteria with promising fiber degrading ability. Fibrolytic enzyme activity is strongly influenced by diet and host species which till date remains unexplored for harnessing their optimum benefits. The present study was conducted to isolate and characterize fiber degrading anaerobic bacteria from faeces of wild blue bull (Boselaphus tragocamelus) inhabiting in semiarid regions. A total of 167 isolates were obtained from 85 faecal samples using M-10 medium, on the basis of clear zones formed on Congo red plates 20 isolates were selected and designated as NLG1-20 for microscopic and biochemical characterization. Further, molecular confirmation was done by PCR analysis with universal 16S rDNA primers. All isolates were obligatory anaerobes except, NLG4, NLG19 and NLG20. Majority of the sugars tested were utilized by most of the isolates except arabinose. Fibrolytic enzyme activities revealed that NLG1 had highest endoglucanase activity, NLG13 had highest exoglucanase activity while NLG8 showed maximum xylanase activity. In case of FPase assay, highest and lowest values were observed in isolate NLG11 (8.96 U/mL) and NLG8 (5.58 U/mL), respectively. Phylogenetic analysis of the isolates revealed a highly diverse group of microbes mainly belonging to the family Paenibacillaceae which have not been previously characterized in ruminants for fiber degradation. Therefore, results obtained in the present study indicated that the screened isolates showed promising fiber degrading potential in terms of filter paper assay and fibrolytic enzyme activity which can be explored further for improving lignocellulose digestibility in ruminants as an additive.

Utilization of fruit and vegetable waste as an alternative feed resource for sustainable and eco-friendly sheep farming.

Globally, 10-20% of horticultural wastes are disposed in landfills leading to environmental pollution. Recycling these wastes as animal feedstuff will lessen food-feed competition and minimize environmental hazards. The present study was undertaken to determine the nutritional quality of fresh fruit and vegetable waste (F&VW) and their dietary inclusion on nutrient utilization, antioxidant status, greenhouse gases (GHG) emissions and potable water sparing efficacy in sheep. Three dietary combinations were formulated i.e. control (C):70% Cenchrus ciliaris hay +30% concentrate mixture (CM), diet with fruit waste (FWD):70% Cenchrus ciliaris hay +20% CM +10% FW and diet with vegetable waste (VWD):70% Cenchrus ciliaris hay +20% CM +10% VW for in vitro and in vivo evaluation of these wastes as potential livestock feed. Twenty-one adult ewes were allocated into 3 groups C, FWD and VWD and fed on the above three diets. Dry matter and crude protein digestibility were significantly enhanced by 5.5 and 7.2%; 7.3 and 7.6% in F&VW supplemented groups, respectively, without affecting feed intake. Plasma total antioxidant capacity (TAC) was improved by 32.2 and 26.3% in F&VW supplemented groups. Inclusion of F&VW biomass reduced annual methane (CH4) and nitrous oxide (N2O) emissions (kg CO2eq/sheep) by 3.12 and 4.55%; 15.18 and 14.92% and thus contributed to lowering of global warming potential by 4.00 and 5.27%, respectively. Furthermore, there was a net reduction of potable water consumption by 21.78 and 13.92% in F&VW supplemented groups, respectively. Therefore, it can be concluded that F&VW can be a potential feedstuff for ruminants and its efficient reuse would minimize environmental impacts associated with disposal of such waste in the landfills.

Effect of drinking earthen pot water on physiological response and behavior of sheep under heat stress.

Heat stress is a major limiting factor for animal welfare and sheep production. Traditionally in India, the villagers used to keep their drinking water in the earthen pot to make it cold during summer. The cold drinking water (24-28 °C) during summer gives a feeling of relief from the heat. Therefore, the present study was carried out to assess the effect of drinking earthen pot water on physiological response and behavior of sheep under heat stress for one month. For this purpose, eighteen Avishaan rams were selected from the experimental animal flock and they were equally divided into three groups; viz., control (CON), heat stress (HS) and heat stress with earthen pot water (HSC). The animals of HS and HSC were exposed to higher ambient temperatures to induce heat stress inside the psychometric chamber. The animals of CON and HS were provided with ad-libitum water of their ambient temperature whereas; HSC groups were provided with ad-libitum cold water (24-28 °C) earthen pot water. All the animals were offered with 400 gm concentrate mixture and ad-libitum Cenchrus hay. The bodyweight of HS rams was significantly reduced (P < 0.05) at the end of the experimental period as compared to their initial body weight. The total roughage and dry matter intake was significantly higher (P < 0.01) in HSC rams as compared to HS rams. The plasma thyroxine concentration was significantly lower (P < 0.05) in HSC as compared with HS group. The rumination time significantly reduced (P < 0.05) in HSC group. However, The blood biochemical did not differ among the groups. Therefore, it may be concluded that Avishaan rams have the ability to adapt to heat stress. Nevertheless, the availability of earthen pot cold drinking water under heat stress reduced their body weight loss, improves their metabolic activity and ultimately improves their welfare.

Effect of complete feed block with tree leaves rich in hydrolysable and condensed tannins on nutrient utilization, rumen fermentation and growth performance of lambs.

Thirty lambs (Avishaan genotype, 10 weeks old, 14.0 ± 0.2 kg live weight) were randomly assigned into three equal treatment groups to study the effect of complete feed blocks (CFB; concentrate and roughage at 70:30 ratio) with three different tanniniferous tree leaves on intake and utilization of nutrients, rumen fermentation, microbial protein synthesis and growth performance. The treatments were T1 (Control), CFB1 with Vigna sinensis hay; T2, CFB2 with Acacia nilotica leaves rich in hydrolysable tannins (HT); and T3, CFB3 with Ziziphus nummularia leaves rich in condensed tannins (CT). The three CFBs were fed ad libitum to the respective groups of lambs for a period of 12 weeks. There was lower (p < .05) intake of dry matter (DM), total carbohydrates (TCHO) and fibre components in T2 compared with T1 and T3. However, the digestibility of nutrients except crude protein (CP) was higher in T2. Diet had no effect (p > .05) on the LW gain in lambs. Amongst the three groups, T3 showed enhanced N utilization with a comparable microbial protein synthesis, the lowest being in T2. The T2 group of lambs had higher propionate and lower non-glucogenic: glucogenic short-chain fatty acids ratio. It may be concluded that tanniniferous tree leaves at 30% of total mixed ration can meet the requirement of nutrients for desired post-weaning growth.