A cavity ringdown spectrometer for methane isotope analysis using a 1.65 µm distributed feedback diode laser with fiber optical feedback loop.

The development of a 1.65 µm cavity ringdown methane spectrometer for methane isotope analysis is reported. In order to reduce the laser linewidth, simple optical feedback with an 11 m external fiber cavity using a retroreflector was implemented and it improved the sensitivity. The detection limit at the ppt level for both 12CH4 and 13CH4 concentrations at 100 Torr gas pressure was evaluated from the Allan-Werle plot calculated from the dataset obtained at the fixed laser frequency. In contrast, the detection limit estimated from the baseline noise on the absorption spectrum was a few ppb for both methane isotopologues due to the periodic background oscillations that remained even after baseline correction. The system demonstrated the direct measurement of ambient methane in atmospheric room air, and the estimated 13CH4 ratio as well as the methane concentration were in good agreement with the reference values of ambient air.

Unique distribution of deep groundwater bacteria constrained by geological setting.

We collected groundwater samples at depths of up to 482 m from three boreholes drilled into sedimentary rock within two formations in Hokkaido, Japan. The prokaryotic community in each subsurface groundwater sample was analysed by microscopic counts and cloning-sequencing the 16S rRNA genes. On total direct counts, there were between 4.61 × 10(4) and 5.06 × 10(6) prokaryote cells ml(-1) in the samples, which is similar to the numbers observed at the marine subsurface. However, the vertical distribution of the prokaryotes did not show a simple decrease in abundance with increasing depth. A high abundance of cells with significant amounts of RNA was identified in the domain Bacteria using fluorescence in situ hybridization, with a high frequency of dividing cells at the transition zone between the two sedimentary rock formations. Cloning-sequencing analysis showed the predominance of γ-Proteobacteria at this transition zone at 281-312 m. The horizontal heterogeneity of the microbial distribution in the subsurface environment was also demonstrated by a relatively high density of members of the domain Archaea in borehole HDB-4, drilled only 1.5 km northeast of HDB-6 and in the same formation.