Effects of Coriander on the Repair Process of Experimentally-induced Periodontitis in Rats.

The aim of this study was to evaluate the effects of Coriandrum sativum L. (CSL) seed extract on gingival levels of antioxidant enzymes, pro-inflammatory cytokines and on alveolar bone and attachment levels after experimental periodontitis induction in rats and compare it with low-dose doxycycline (LDD). Forty adult male Wistar Albino rats were divided randomly into 5 groups as follows: 1 = periodontally healthy (control); 2 = periodontitis; 3 = periodontitis + CSL (32 mg/kg); 4 = periodontitis + CSL (200 mg/kg); and 5 = periodontitis + LDD (6 mg/kg). Gingival superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) levels were evaluated by enzyme-linked immunosorbent assay. The presence of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), and interleukin-1ßeta (IL-1ß) immunoreactivity was detected immunohistochemically. Alveolar bone area in the furcation space (ABA), alveolar bone loss (ABL), and attachment loss (AL) were evaluated histomorphometrically. The SOD level was lower in group 5 than in groups 2, 3, and 4. The IL-1ß level was highest in group 4. The TNF-α level was statistically higher in groups 2 and 4 than in groups 1, 3, and 5. The IL-6 level was highest in group 4. Its level was higher in groups 2 and 3 than in group 5. ABA was less in groups 2, 3, and 4 compared to groups 1 and 5. ABL was less in group 5 than in groups 2, 3, and 4. AL was greater in group 4 than in group 5. The use of 200 mg/kg CSL showed a pro-inflammatory effect and IL-1ß and TNF-α levels decreased after 32 mg/kg CSL application in the treatment of periodontitis.

Evaluation of Clinical Parameters and Levels of Proinflammatory Cytokines in the Crevicular Fluid Around Dental Implants in Patients with Type 2 Diabetes Mellitus.

PURPOSE: Diabetes mellitus is considered a relative contraindication for dental implant therapy, depending on levels of glycemic control. The purpose of this research was to evaluate the peri-implant conditions and measure the levels of interleukin 1 beta (IL-1ß) and tumor necrosis factor alpha (TNF-α) in the peri-implant crevicular fluid (PICF) in patients with glycemic-controlled type 2 diabetes (T2DM). MATERIALS AND METHODS: Thirteen patients with well-controlled T2DM and seven systemically healthy patients were recruited for this study. Clinical measurements were recorded, and samples of gingival crevicular fluid (GCF) and PICF were collected from the sulci of both adjacent teeth and implants at baseline (after implant placement), 1 month, 4 months, and 7 months. Levels of IL-1ß and TNF-α in the GCF and PICF were analyzed by enzyme-linked immunosorbent assay. Standardized intraoral radiographs were taken at baseline (after implant placement), 4 months, and 7 months. Implant stability was assessed by resonance frequency analysis at baseline, at 1 month, and at 4 months. RESULTS: Glycosylated hemoglobin levels in the T2DM group were significantly increased at 7 months compared to baseline levels. Implant stability quotient levels at 4 months were increased significantly compared to baseline in both groups. Alveolar bone levels around implants were statistically lower at 4 and 7 months compared to baseline and statistically decreased at 7 months compared to 4 months in the T2DM group. There were no significant differences between groups in bone levels around implants, and no significant differences were found in levels of IL-1ß and TNF-α at either teeth or implants or between the groups. CONCLUSION: The results suggest that dental implant therapy can be offered to patients with well-controlled T2DM, as there were no significant differences between control and diabetic patients in terms of clinical parameters or GCF and PICF cytokine levels.