Assigning harvested waterfowl to geographic origin using feather δ2H isoscapes: What is the best analytical approach?

Establishing links between breeding, stopover, and wintering sites for migratory species is important for their effective conservation and management. Isotopic assignment methods used to create these connections rely on the use of predictable, established relationships between the isotopic composition of environmental hydrogen and that of the non-exchangeable hydrogen in animal tissues, often in the form of a calibration equation relating feather (δ2Hf) values derived from known-origin individuals and amount-weighted long-term precipitation (δ2Hp) data. The efficacy of assigning waterfowl to moult origin using stable isotopes depends on the accuracy of these relationships and their statistical uncertainty. Most current calibrations for terrestrial species in North America are done using amount-weighted mean growing-season δ2Hp values, but the calibration relationship is less clear for aquatic and semi-aquatic species. Our objective was to critically evaluate current methods used to calibrate δ2Hp isoscapes to predicted δ2Hf values for waterfowl. Specifically, we evaluated the strength of the relationships between δ2Hp values from three commonly used isoscapes and known-origin δ2Hf values three published datasets and one collected as part of this study, also grouping these data into foraging guilds (dabbling vs diving ducks). We then evaluated the performance of assignments using these calibrations by applying a cross-validation procedure. It remains unclear if any of the tested δ2Hp isoscapes better predict surface water inputs into food webs for foraging waterfowl. We found only marginal differences in the performance of the tested known-origin datasets, where the combined foraging-guild-specific datasets showed lower assignment precision and model fit compared to data for individual species. We recommend the use of the more conservative combined foraging-guild-specific datasets to assign geographic origin for all dabbling duck species. Refining these relationships is important for improved waterfowl management and contributes to a better understanding of the limitations of assignment methods when using the isotope approach.

Long-Distance Migration of the Globe Skimmer Dragonfly to Japan Revealed Using Stable Hydrogen (δ 2H) Isotopes.

The globe skimmer dragonfly, Pantala flavescens Fabricius (Odonata: Libellulidae), is a long-distance migrant, well adapted to exploiting ephemeral waterbodies. This species occurs in Japan every summer, but overwintering has only been recorded on subtropical Ishigaki Island. It is not known from where the summer immigrants originate, nor what proportion of the globe skimmers seen in Japan are of local origin. We analyzed stable hydrogen isotope (δ 2H) composition of wings of 189 P. flavescens captured at six sites in Japan from August to September in 2016 (n = 57) and from April to November in 2017 (n = 132). We determined that the majority of individuals were immigrants. Individuals of probable Japanese origin occurred only later in the year and were of lower mass on average than immigrants. Immigrants potentially originated from a broad area as far west as northern India and the Tibetan Plateau and, especially late in the season, as near as northcentral China and the Korean peninsula. However, for April samples, the most parsimonious interpretation suggested southern origins, in northern Myanmar to southern China, or possibly Borneo-Sulawesi. Our investigation underlines the power of combining stable isotope data with other information such as wind speed and direction, arrival dates, and body mass to estimate origins and to understand the life history of this and other insects.

Effects of agricultural intensification on nestling condition and number of young fledged of barn swallows (Hirundo rustica).

Farmland bird populations have declined with increasing agricultural intensification possibly due to putative reductions in prey insects and effects of pesticide exposure. Presence of agriculture may be especially relevant for aerial insectivorous songbirds whose primary diet is flying insects. Here, we investigated the effects of agricultural land use on nestling body condition, an important determinant of post-fledging survival, for barn swallows (Hirundo rustica), an aerial insectivore breeding within an agro-ecosystem in southern Ontario, Canada. Our scale-of-effect analysis revealed that nestling and pre-fledging body condition varied most strongly with the proportion of row crop within 100 m of the natal barn. Unexpectedly, this correlation was positive for both nestling body condition (2016 only) and for pre-fledging condition (2016 and 2017). We found a weak positive effect of row crop on number of young fledged. We speculate that the positive effects of agricultural row-cropping on condition and number of young fledged was due to higher prey availability and/or more open foraging habitat around barns surrounded by row crops. Alternatively, higher nestling condition in high agriculture environments could reflect an insurance policy to increase survival during the post-fledging period. Our results suggest that, in our southern Ontario study area, the degree of agricultural conversion does not negatively influence individual nestling condition and number of young produced for barn swallows. We recommend future research on this species to examine reproductive success in more intense agricultural landscapes and possible effects of pesticide exposure.