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1.
Biol Reprod ; 100(2): 535-546, 2019 02 01.
Artigo em Inglês | MEDLINE | ID: mdl-30252024

RESUMO

A stable system for producing sterile domesticated fish is required to prevent genetic contamination to native populations caused by aquaculture escapees. The objective of this study was to develop a system to mass produce stock for aquaculture that is genetically sterile by surrogate broodstock via spermatogonial transplantation (SGTP). We previously discovered that female medaka carrying mutations on the follicle-stimulating hormone receptor (fshr) gene become sterile. In this study, we demonstrated that sterile hybrid recipient females that received spermatogonia isolated from sex-reversed XX males (fshr (-/-)) recovered their fertility and produced only donor-derived fshr (-) X eggs. Natural mating between these females and fshr (-/-) sex-reversed XX males successfully produced large numbers of sterile fshr (-/-) female offspring. In conclusion, we established a new strategy for efficient mass production of sterile fish. This system can be applied to any aquaculture species for which SGTP and methods for producing sterile recipients can be established.


Assuntos
Infertilidade/veterinária , Oryzias/genética , Oryzias/fisiologia , Receptores do FSH/genética , Espermatogônias/fisiologia , Animais , Inibidores da Aromatase/farmacologia , Feminino , Deleção de Genes , Regulação da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica/genética , Genótipo , Hibridização Genética , Infertilidade/genética , Masculino
2.
Sci Rep ; 7: 43185, 2017 03 03.
Artigo em Inglês | MEDLINE | ID: mdl-28256523

RESUMO

The medaka (Oryzias latipes) is a teleost model distinguished from other model organisms by the presence of inbred strains, wild stocks, and related species. Cryopreservation guarantees preservation of these unique biological resources. However, because of their large size, cryopreservation techniques for their eggs and embryos have not been established. In the present study, we established a methodology to produce functional gametes from cryopreserved testicular cells (TCs). Whole testes taken from medaka were cryopreserved by vitrification. After thawing, the cells dissociated from cryopreserved testicular tissues were intraperitoneally transplanted into sterile triploid hatchlings. Some cells, presumably spermatogonial stem cells, migrated into the genital ridges of recipients and resulted in the production of eggs or sperm, based on sex of the recipient. Mating of recipients resulted in successful production of cryopreserved TC-derived offspring. We successfully produced individuals from the Kaga inbred line, an endangered wild population in Tokyo, and a sub-fertile mutant (wnt4b-/-) from cryopreserved their TCs. This methodology facilitates semi-permanent preservation of various medaka strains.


Assuntos
Transplante de Células/métodos , Criopreservação/métodos , Células Germinativas/fisiologia , Células Germinativas/efeitos da radiação , Oryzias/crescimento & desenvolvimento , Testículo/citologia , Vitrificação , Animais , Masculino
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