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1.
Nutrients ; 16(16)2024 Aug 13.
Artigo em Inglês | MEDLINE | ID: mdl-39203808

RESUMO

This randomised double-blind placebo-controlled trial evaluated the efficacy and safety of fermented gold kiwi (FGK) in improving gastrointestinal health. A total of 100 participants were enrolled and randomly assigned to treatment or placebo groups. Over 8 weeks, the participants consumed an FGK or placebo preparation daily. Primary outcomes included changes in gastrointestinal symptoms assessed using the Gastrointestinal Symptom Rating Scale (GSRS) and the Korean version of the Nepean Dyspepsia Index (NDI-K), as well as quality of life assessed using the Functional Dyspepsia-related Quality of Life questionnaire. The FGK group showed significant improvements in GSRS and NDI-K total and subdomain scores compared with the placebo group. Moreover, the quality of life scores were significantly better in the FGK group than in the placebo group. Safety evaluations revealed no significant adverse events or clinically meaningful changes upon assessing laboratory test results. This study demonstrated that FGK is a safe and effective dietary supplement for improving gastrointestinal health in adults with gastrointestinal symptoms.


Assuntos
Dispepsia , Qualidade de Vida , Humanos , Método Duplo-Cego , Feminino , Masculino , Adulto , Pessoa de Meia-Idade , Dispepsia/tratamento farmacológico , Alimentos Fermentados , Resultado do Tratamento , Suplementos Nutricionais , Fermentação
2.
Lipids Health Dis ; 23(1): 192, 2024 Jun 22.
Artigo em Inglês | MEDLINE | ID: mdl-38909257

RESUMO

BACKGROUND: Overweight, often known as obesity, is the abnormal and excessive accumulation of fat that exposes the health of a person at risk by increasing the likelihood that they may experience many chronic conditions. Consequently, obesity has become a global health threat, presenting serious health issues, and attracting a lot of attention in the healthcare profession and the scientific community. METHOD: This study aims to explore the anti-adipogenic properties of 7-MEGA™ in an attempt to address obesity, using both in vitro and in vivo research. The effects of 7MEGA™ at three distinct concentrations were investigated in obese mice who were given a high-fat diet (HFD) and 3T3-L1 adipocytes. RESULTS: 7MEGA™ decreased the total fat mass, overall body weight, and the perirenal and subcutaneous white adipose tissue (PWAT and SWAT) contents in HFD mice. Additionally, 7MEGA™ showed promise in improving the metabolic health of individuals with obesity and regulate the levels of insulin hormone, pro-inflammatory cytokines and adipokines. Furthermore, Peroxisome proliferator-activated receptors (PPAR) α and γ, Uncoupling Protein 1 (UCP-1), Sterol Regulatory Element-Binding Protein 1 (SREBP-1), Fatty Acid-Binding Protein 4 (FABP4), Fatty Acid Synthase (FAS), Acetyl-CoA Carboxylase (ACC), Stearoyl-CoA Desaturase-1 (SCD-1) and CCAAT/Enhancer-Binding Protein (C/EBPα) were among the adipogenic regulators that 7MEGA™ could regulate. CONCLUSION: In summary, this study uncovered that 7MEGA™ demonstrates anti-adipogenic and anti-obesity effects, suggesting its potential in combating obesity.


Assuntos
Células 3T3-L1 , Adipócitos , Adipogenia , Dieta Hiperlipídica , Camundongos Endogâmicos C57BL , Obesidade , Animais , Dieta Hiperlipídica/efeitos adversos , Adipogenia/efeitos dos fármacos , Obesidade/metabolismo , Camundongos , Adipócitos/efeitos dos fármacos , Adipócitos/metabolismo , Masculino , PPAR gama/metabolismo , PPAR gama/genética , Proteína de Ligação a Elemento Regulador de Esterol 1/metabolismo , Proteína de Ligação a Elemento Regulador de Esterol 1/genética , Estearoil-CoA Dessaturase/metabolismo , Estearoil-CoA Dessaturase/genética , Camundongos Obesos , Proteínas de Ligação a Ácido Graxo/metabolismo , Proteínas de Ligação a Ácido Graxo/genética , Adipocinas/metabolismo , Fármacos Antiobesidade/farmacologia , Proteína Desacopladora 1/metabolismo , Proteína Desacopladora 1/genética , Tecido Adiposo Branco/metabolismo , Tecido Adiposo Branco/efeitos dos fármacos , Proteínas Estimuladoras de Ligação a CCAAT
3.
Biomed Pharmacother ; 175: 116700, 2024 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-38703505

RESUMO

Late-onset hypogonadism (LOH) is an age-related disease in men characterized by decreased testosterone levels with symptoms such as decreased libido, erectile dysfunction, and depression. Thymus quinquecostatus Celakovski (TQC) is a plant used as a volatile oil in traditional medicine, and its bioactive compounds have anti-inflammatory potential. Based on this knowledge, the present study aimed to investigate the effects of TQC extract (TE) on LOH in TM3 Leydig cells and in an in vivo aging mouse model. The aqueous extract of T. quinquecostatus Celakovski (12.5, 25, and 50 µg/mL concentrations) was used to measure parameters such as cell viability, testosterone level, body weight, and gene expression, via in vivo studies. Interestingly, TE increased testosterone levels in TM3 cells in a dose-dependent manner without affecting cell viability. Furthermore, TE significantly increased the expression of genes involved in the cytochrome P450 family (Cyp11a1, Cyp17a1, Cyp19a1, and Srd5a2), which regulate testosterone biosynthesis. In aging mouse models, TE increased testosterone levels without affecting body weight and testicular tissue weight tissue of an aging animal group. In addition, the high-dose TE-treated group (50 mg/kg) showed significantly increased expression of the cytochrome p450 enzymes, similar to the in vitro results. Furthermore, HPLC-MS analysis confirmed the presence of caffeic acid and rosmarinic acid as bioactive compounds in TE. Thus, the results obtained in the present study confirmed that TQC and its bioactive compounds can be used for LOH treatment to enhance testosterone production.


Assuntos
Envelhecimento , Extratos Vegetais , Testículo , Testosterona , Thymus (Planta) , Animais , Testosterona/sangue , Masculino , Envelhecimento/efeitos dos fármacos , Envelhecimento/metabolismo , Camundongos , Extratos Vegetais/farmacologia , Testículo/efeitos dos fármacos , Testículo/metabolismo , Thymus (Planta)/química , Células Intersticiais do Testículo/efeitos dos fármacos , Células Intersticiais do Testículo/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Linhagem Celular , Hipogonadismo/tratamento farmacológico , Modelos Animais de Doenças
4.
Nutrients ; 16(2)2024 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-38257104

RESUMO

7-MEGATM is a food product made from purified Alaska pollack fish oil containing palmitoleic acid (16:1), commonly referred to as omega-7. We sought to quantitatively evaluate whether this substance inhibits skin aging. A total of 101 middle-aged females were randomly allocated to the intervention (N = 50) or placebo group (N = 51). Each participant was advised to take either 500 mg of 7-MEGATM or a placebo twice daily for 12 weeks. The primary outcomes were the degree of improvement in wrinkles and the degree of moisture filling after consumption for 12 weeks compared to baseline. The secondary outcomes were improvement in skin wrinkles; moisture changes at 4 and 8 weeks from baseline; changes in transdermal water loss, skin elasticity, the melanin index, the erythema index, and the Global Photo Damage Score. We found a significant improvement in skin wrinkles and elasticity at 12 weeks in the 7-MEGATM-consuming group compared to that in the placebo group; skin moisture, elasticity, and the melanin index were also improved. No supplement-related adverse reactions were observed and 7-MEGATM was identified as safe. 7-MEGATM was effective for human skin function in terms of wrinkles, moisture, elasticity, and melanin production and may be useful as a skin nutritional supplement.


Assuntos
Envelhecimento da Pele , Feminino , Humanos , Pessoa de Meia-Idade , Suplementos Nutricionais , Elasticidade , Melaninas , Pele , Método Duplo-Cego
5.
Prev Nutr Food Sci ; 19(4): 343-7, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-25580400

RESUMO

The present study investigated the effects of Vaccinium uliginosum L. (bilberry) on the learning and memory impairments induced by amyloid-ß protein (AßP) 1-42. ICR Swiss mice were divided into 4 groups: the control (Aß40-1A), control with 5% bilberry group (Aß40-1B), amyloid ß protein 1-42 treated group (Aß1-42A), and Aß1-42 with 5% bilberry group (Aß1-42B). The control was treated with amyloid ß-protein 40-1 for placebo effect, and Alzheimer's disease (AD) group was treated with amyloid ß-protein 1-42. Amyloid ß-protein 1-42 was intracerebroventricular (ICV) micro injected into the hippocampus in 35% acetonitrile and 0.1% trifluoroacetic acid. Although bilberry added groups tended to decrease the finding time of hidden platform, no statistical significance was found. On the other hand, escape latencies of AßP injected mice were extended compared to that of Aß40-1. In the Probe test, bilberry added Aß1-42B group showed a significant (P<0.05) increase of probe crossing frequency compared to Aß1-42A. Administration of amyloid protein (Aß1-42) decreased working memory compared to Aß40-1 control group. In passive avoidance test, bilberry significantly (P<0.05) increased the time of staying in the lighted area compared to AD control. The results suggest that bilberry may help to improve memory and learning capability in chemically induced Alzheimer's disease in experimental animal models.

6.
J Cosmet Dermatol ; 12(4): 287-95, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24305427

RESUMO

BACKGROUND: Epidermal and fibroblast growth factor (EGF and FGF1) proteins play an important role in the regeneration and proliferation of skin cells. EGF and FGF1 have considerable potential as possible therapeutic or cosmetic agents for the treatment of skin damage including wrinkles. OBJECTIVES: Using protein transduction domains (PTD), we investigated whether PTD-EGF and FGF1 transduced into skin cells and tissue. Transduced proteins showed protective effects in a UV-induced skin damage model as well as against skin wrinkles. METHODS: Transduced PTD-EGF and FGF1 proteins were detected by immunofluorescence and immunohistochemistry. The effects of PTD-EGF and FGF1 were examined by WST assay, Western blotting, immunohistochemistry, and skin wrinkle parameters. RESULTS: The PTD-EGF and FGF1 increased cell proliferation and collagen type 1 alpha 1 protein accumulation in skin tissue. Also, PTD-EGF and FGF1 inhibited UV-induced skin damage. Furthermore, topical application of PTD-EGF and FGF1 contained ampoules which were considered to improve the wrinkle parameters of human skin. CONCLUSION: These results show that PTD-EGF and FGF1 can be a potential therapeutic or cosmetic agent for skin damaged and injury including wrinkles and aging.


Assuntos
Fármacos Dermatológicos/farmacologia , Fator de Crescimento Epidérmico/farmacologia , Fator 1 de Crescimento de Fibroblastos/farmacologia , Envelhecimento da Pele/efeitos da radiação , Pele/efeitos dos fármacos , Raios Ultravioleta/efeitos adversos , Animais , Modelos Animais de Doenças , Humanos , Camundongos , Camundongos Endogâmicos C57BL , Resultado do Tratamento
7.
Inflamm Bowel Dis ; 15(8): 1164-73, 2009 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-19334074

RESUMO

BACKGROUND: 3,3-Diindolylmethane (DIM) is a major in vivo product of acid-catalyzed oligomerization of indole-3-carbinol (I3C) derived from Brassica food plants. Although DIM is known as a chemopreventive and chemotherapeutic phytochemical, the effects of DIM on inflammation in vivo are still unknown. In the present study we investigated the antiinflammatory effects of DIM on experimental colitis and colitis-associated colorectal carcinogenesis. METHODS: To determine if DIM has an antiinflammatory effect in vivo, we examined the therapeutic effects of DIM in dextran sodium sulfate (DSS)-induced experimental colitis and colitis-associated colon carcinogenesis induced by azoxymethane (AOM)/DSS in BALB/c mice. RESULTS: Treatment with DIM significantly attenuated loss of body weight, shortening of the colon, and severe clinical signs in a colitis model. This was associated with a remarkable amelioration of the disruption of the colonic architecture and a significant reduction in colonic myeloperoxidase activity and production of prostaglandin E(2), nitric oxide, and proinflammatory cytokines. Further, DIM administration dramatically decreased the number of colon tumors in AOM/DSS mice. CONCLUSIONS: These results suggest that DIM-mediated antiinflammatory action at colorectal sites may be therapeutic in the setting of inflammatory bowel disease and colitis-associated colon cancer.


Assuntos
Anticarcinógenos/uso terapêutico , Colite/prevenção & controle , Neoplasias do Colo/prevenção & controle , Indóis/uso terapêutico , Animais , Azoximetano/toxicidade , Peso Corporal/efeitos dos fármacos , Carcinógenos/toxicidade , Transformação Celular Neoplásica , Colite/induzido quimicamente , Colite/patologia , Neoplasias do Colo/induzido quimicamente , Neoplasias do Colo/patologia , Sulfato de Dextrana/toxicidade , Dinoprostona/metabolismo , Modelos Animais de Doenças , Camundongos , Camundongos Endogâmicos BALB C , NF-kappa B/metabolismo , Óxido Nítrico/metabolismo , Peroxidase/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Redução de Peso/efeitos dos fármacos
8.
Biochem Biophys Res Commun ; 381(4): 502-7, 2009 Apr 17.
Artigo em Inglês | MEDLINE | ID: mdl-19233127

RESUMO

Chrysin (5,7-dihydroxyflavone) is a natural flavone commonly found in many plants. It has previously been shown to be an anti-tumor agent. In this study, we investigated whether chrysin could alleviate the symptoms of dextran sodium sulfate (DSS)-induced colitis in mice and whether chrysin has an inhibitory effect on nuclear factor (NF)-kappaB activation in vitro. A significant blunting of weight loss and clinical signs was observed in DSS-exposed, chrysin-treated mice when compared to vehicle-treated mice. This was associated with a remarkable amelioration of the disruption of the colonic architecture, a significant reduction in colonic myeloperoxidase (MPO) activity, and a decrease in the production of inflammatory mediators such as nitric oxide (NO), prostaglandin (PG) E(2), and pro-inflammatory cytokines. In addition, chrysin inhibited tumor necrosis factor (TNF)-alpha-induced activation of NF-kappaB in IEC-6 cells. These findings suggest that chrysin exerts potentially clinically useful anti-inflammatory effects mediated through the suppression of NF-kappaB activation.


Assuntos
Colo/efeitos dos fármacos , Flavonoides/uso terapêutico , Doenças Inflamatórias Intestinais/tratamento farmacológico , Animais , Colo/metabolismo , Colo/patologia , Sulfato de Dextrana/toxicidade , Modelos Animais de Doenças , Proteínas I-kappa B/metabolismo , Mediadores da Inflamação/metabolismo , Doenças Inflamatórias Intestinais/induzido quimicamente , Doenças Inflamatórias Intestinais/patologia , Mucosa Intestinal/efeitos dos fármacos , Mucosa Intestinal/metabolismo , Mucosa Intestinal/patologia , Camundongos , Camundongos Endogâmicos BALB C , Transporte Proteico/efeitos dos fármacos , Redução de Peso/efeitos dos fármacos
9.
J Mol Med (Berl) ; 86(11): 1287-95, 2008 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-18825356

RESUMO

Licochalcone A (LicA), a major phenolic constituent of the licorice species Glycyrrhiza inflata, exhibits various biological properties, including chemopreventive, anti-bacterial, and anti-spasmodic activity. We report that LicA inhibits inflammatory reactions in macrophages and protects mice from endotoxin shock. Our in vitro experiments showed that LicA suppressed not only the generation of nitric oxide (NO) and prostaglandin (PG)E(2), but also the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 induced by lipopolysaccharide (LPS) in RAW264.7 cells. Similarly, LicA inhibited the production of inflammatory cytokines induced by LPS in RAW264.7 cells, including IL-1 beta and IL-6. In an animal model, LicA protected BALB/c mice from LPS-induced endotoxin shock, possibly through inhibiting the production of inflammatory cytokines and NO. Collectively, LicA inhibited the production of inflammatory mediators and may be a potential target for treatment of various inflammatory diseases.


Assuntos
Anti-Inflamatórios não Esteroides/farmacologia , Chalconas/farmacologia , Glycyrrhiza/química , Lipopolissacarídeos/farmacologia , Choque Séptico/tratamento farmacológico , Animais , Anti-Inflamatórios não Esteroides/uso terapêutico , Linhagem Celular , Chalconas/isolamento & purificação , Chalconas/uso terapêutico , Inflamação/tratamento farmacológico , Inflamação/metabolismo , Mediadores da Inflamação/metabolismo , Camundongos , NF-kappa B/fisiologia , Choque Séptico/metabolismo , Choque Séptico/mortalidade , Proteínas Quinases p38 Ativadas por Mitógeno/fisiologia
10.
Int Immunopharmacol ; 8(12): 1695-702, 2008 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-18773974

RESUMO

Piceatannol (3,5,3',4'-tetrahydroxy-trans-stilbene; PIC) is a polyphenol found in grapes. It is known as a protein kinase inhibitor that modifies multiple cellular targets, exerting immunosuppressive and antitumorigenic activities in several cell lines. The purpose of the present work was to evaluate the anti-inflammatory effect of PIC on dextran sulfate sodium (DSS)-induced colitis. Experimental colitis was induced in BALB/c mice by dissolving 5% DSS in their drinking water for 7 days. PIC (1, 2.5, 5, or 10 mg/kg body weight) was administrated daily per oral route for 7 days. A significant blunting of weight loss and clinical signs was observed in DSS-exposed, PIC-treated mice when compared to vehicle-treated mice. This was associated with a remarkable amelioration of the disruption of the colonic architecture, a significant reduction in colonic myeloperoxidase (MPO) activity, and a decrease in production of inflammatory mediators such as nitric oxide (NO), prostaglandin (PG) E2, and pro-inflammatory cytokines. The present data indicate that further evaluation of the potential of PIC as an agent for the prevention and/or treatment of inflammatory bowel diseases in human clinical studies is warranted.


Assuntos
Colite/tratamento farmacológico , Estilbenos/uso terapêutico , Vitis/química , Animais , Colite/induzido quimicamente , Colite/imunologia , Colite/patologia , Colo/imunologia , Sulfato de Dextrana/toxicidade , Feminino , Imuno-Histoquímica , Mediadores da Inflamação/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , NF-kappa B/análise , Peroxidase/metabolismo , Fator de Transcrição STAT3/análise
11.
Biochem Biophys Res Commun ; 345(3): 1215-23, 2006 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-16716255

RESUMO

Licorice, the roots of Glycyrrhiza inflata, is used by practitioners of alternative medicine to treat individuals with gastric or duodenal ulcers, bronchitis, cough, arthritis, adrenal insufficiency, and allergies. We investigated the anti-inflammatory properties of 4 licorice extracts: extracts of roasted licorice obtained by ethanol (rLE) or water extraction (rLW) and extracts of raw licorice obtained by ethanol (LE) or water extraction (LW). rLE demonstrated strong anti-inflammatory activity through its ability to reduce nitric oxide and prostaglandin E(2) production in the LPS-stimulated mouse macrophage cell, RAW264.7. It also inhibited the production of pro-inflammatory cytokines and CD14 expression on the LPS-stimulated RAW264.7 cells. Further study indicated that LPS-induced degradation and phosphorylation of Ikappa-Balpha, along with DNA-binding of NF-kappaB, was significantly inhibited by rLE exposure in RAW264.7 cells. In the murine model, we found that in vivo exposure to rLE-induced an increase in the survival rate, reduced plasma levels of TNF-alpha and IL-6, and increased IL-10 production in LPS-treated mice. Collectively, these data suggest that the use of rLE may be a useful therapeutic approach to various inflammatory diseases.


Assuntos
Anti-Inflamatórios/farmacologia , Glycyrrhiza/metabolismo , Lipopolissacarídeos/metabolismo , Macrófagos/metabolismo , Animais , Dinoprostona/metabolismo , Feminino , Proteínas I-kappa B/metabolismo , Inflamação , Receptores de Lipopolissacarídeos/biossíntese , Camundongos , Camundongos Endogâmicos ICR , Inibidor de NF-kappaB alfa , NF-kappa B/metabolismo , Extratos Vegetais
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