High Proliferative Placenta-Derived Multipotent Cells Express Cytokeratin 7 at Low Level.

The purpose of this study was to investigate the immunophenotypes and gene expression profile of high proliferative placenta-derived multipotent cells (PDMCs) population at different stages of culture. We demonstrated that the colonies resulting from single cells were either positive or negative for CK7, whereas only PDMC clones with weak CK7 expression (CK7low-clones) were highly proliferative. Interestingly, vimentin positive (Vim+) placental stromal mesenchymal cells did not express CK7 in situ, but double CK7+Vim+ cells detection in tissue explants and explants outgrowth indicated CK7 inducible expression in vitro. PCNA presence in CK7+Vim+ cells during placental explants culturing confirmed belonging of these cells to proliferative subpopulation. Transcription factors CDX2 and EOMES were expressed in both CK7low-clones and subset of stromal mesenchymal cells of first-trimester placental tissue in situ. Meanwhile, CK7low -clones and stromal mesenchymal cells of full-term placental tissue in situ expressed ERG heterogeneously. SPP1, COL2A1, and PPARG2 mesodermal-related genes expression by CK7low-clones additionally confirms their mesenchymal origin. Inherent stem cell-related gene expression (IFTM3, POU5F1, and VASA) in CK7low-clones might indicate their enrichment for progenitors. Finally, in CK7low-clones we observed expression of such trophoblast-associated genes as CGB types I and II, fusogenic ERVW-1, GCM1, and GATA3. Thus, our results indicate that PDMCs acquired the representative immunophenotype signature under culture conditions.

[Effects of the umbilical blood stem cells in experimental injury of myocardium].

Morphological, functional signs of myocardium affection and tolerance to physical loading in animals with isoproterenol-induced affection of myocardium after transplantation of a nuclear-containing cells (NCC) of umbilical blood were analyzed in comparison with a natural course of the model. There was proved, that a transplantation of a NCC promotes acceleration of processes of regeneration and restoration of the damaged myocardium in experimental animals. There were estimated the changes in localization of the transplanted NCC in the zone of damage, and capacity of the peripheral blood NCC, while they were transplanted, using intravenous injection, to migrate into the damage zone. The experimental data obtained need further complex analysis and permit to wait a positive effect while performing clinical investigations in the patients, suffering chronic diseases of the heart.

[Perspectives of application of allogeneic multipotent mesenchymal stem cells for the adipose transplant protection from tissue resorption].

The detection of possibility differentiation towards adipogenic trend of the cells, which were obtained from adipose tissue, and their impact on the processes of adipose transplant resorption, in condition of its allogenic transplantation, have had constituted the objective of the investigation. Combined transplantation of adipose transplant and multipotent mesenchymal stem cells, obtained from allogenic adipose tissue, causes the destruction-inflammatory processes activation in the adipose transplant, as well as the adipocytes survival worsening in a meanwhile - a significant deficiency in the transplant mass.

[Preservation of parenchymal and stromal progenitors in cryopreserved human fetal liver].

In this paper we show presents of viable population of hepatoblasts, endodermal blasts, endothelial and mesenchymal cells in the cryopreserved suspension cells of human fetal liver. Also we observed epithelial-mesenchymal transition of hepatoblasts in culture. We show that it's possible to apply the method of cryopreservation of hematopoietic cells of human fetal liver of the first gestation trimester for cryopreservation of parenchymal and stromal cells of fetal liver.

[Transplantation of the adipose tissue enriched by allogenic mesenchymal multipotent stem cells in experimental conditions].

There was conducted experimental investigation with objective to study up the influence of transplantation of a native allogenic multipotent mesenchymal stem cells on the processes of implantation and functioning of heterotopically transplanted autologous adipose tissue. In a cellular-tissue graft, in which native allogenic mesenchymal stem cells were applied as a cellular component, there was observed a worsening of the adipocytes survival indices as well as activation of destructive and inflammatory processes.

[Migration and differentiation of transplanted fetal neurogenic cells in animals with brain ischemia].

The migration, integration and differentiation of fetal neural progenitor cells (NPCs) in the ischemic brain have been studied. In our study the ischemic insult in FVB line mice was produced by occlusion of both carotid arteries during 20 min. A day after occlusion NPCs from GFP-transgenic 12.5 dpc embryos were suboccipitally transplanted to the ischemic brain. The migration and differentiation of GFP-positive NPCs in the recipient tissue were observed in different time points after their transplantation by immunohistochemical approaches using confocal scanning microscopy. It has been shown that GFP-positive NPCs survived, migrated and differentiated to the mature neurons and glial cells in CA1 area of hippocampus of ischemic animals.