RESUMO
Estimates of the intensity and abundance of species provide essential data for ecological, evolutionary and epidemiological studies of gastrointestinal nematode communities. These estimates are typically derived from the species composition of adult males when only males have readily scorable species-specific morphological traits. Such estimation assumes that all species in the community have the same adult sex ratio. We evaluated this assumption for the trichostrongyle nematodes Ostertagia gruehneri and Marshallagia marshalli in infracommunities in Svalbard reindeer by identifying to species adult females using a polymerase chain reaction assay. The proportion of males was found to be slightly higher in O. gruehneri than in M. marshalli. Evidence for seasonal variation and density dependence in the adult sex ratio was only found for O. gruehneri. Possible demographic mechanisms for such sex ratio variation are discussed, and stochastic models that generate density-dependent sex ratios proposed. Sex ratio variation caused substantial bias in some male-based estimates of intensity of infection, while substantial and consistent bias in estimates of abundances was only evident in late winter samples. Our results suggest that estimating sex ratios can be particularly important in individual host level studies of nematode species of low abundance.
Assuntos
Gastroenteropatias/veterinária , Nematoides/fisiologia , Infecções por Nematoides/veterinária , Rena/parasitologia , Animais , Feminino , Gastroenteropatias/parasitologia , Masculino , Infecções por Nematoides/parasitologia , Estações do Ano , Razão de MasculinidadeRESUMO
Mainland populations of Arctic reindeer and caribou Rangifer tarandus often undergo extensive movements, whereas populations on islands tend to be isolated and sedentary. To characterize the genetic consequences of this difference, levels of genetic diversity and subdivision of Svalbard reindeer (R. t. platyrhynchus) from two adjacent areas on Nordenskjiöldland, Spitsbergen were estimated using data from up to 14 microsatellites. The mean number of alleles per locus in Svalbard reindeer was 2.4 and mean expected heterozygosity per locus was 0.36. The latter value was significantly lower than in Canadian caribou and Norwegian reindeer but higher than in some other cervid species. Large samples of females (n = 743) and small samples of males (n = 38) from two sites approximately 45 km apart showed genetic subdivision, which could be due to local population fluctuations or limited gene flow. To our knowledge, this is the first study to report significant differentiation at microsatellite loci in Rangifer at such short geographical distances. Neither population showed genetic evidence for recent population bottlenecks when loci unbiased with respect to heterozygosity were analysed. In contrast, false signals of a recent bottleneck were detected when loci upwardly biased with respect to heterozygosity were analysed. Thus, Svalbard reindeer appeared to conform to the paradigm of island populations made genetically depauperate by genetic drift.
Assuntos
DNA/análise , Deriva Genética , Variação Genética , Repetições de Microssatélites/genética , Rena/genética , Alelos , Animais , Feminino , Frequência do Gene , Genética Populacional , Heterozigoto , Masculino , Dinâmica Populacional , SvalbardRESUMO
Even though theoretical models show that parasites may regulate host population densities, few empirical studies have given support to this hypothesis. We present experimental and observational evidence for a host-parasite interaction where the parasite has sufficient impact on host population dynamics for regulation to occur. During a six year study of the Svalbard reindeer and its parasitic gastrointestinal nematode Ostertagia gruehneri we found that anthelminthic treatment in April-May increased the probability of a reindeer having a calf in the next year, compared with untreated controls. However, treatment did not influence the over-winter survival of the reindeer. The annual variation in the degree to which parasites depressed fecundity was positively related to the abundance of O. gruehneri infection the previous October, which in turn was related to host density two years earlier. In addition to the treatment effect, there was a strong negative effect of winter precipitation on the probability of female reindeer having a calf. A simple matrix model was parameterized using estimates from our experimental and observational data. This model shows that the parasite-mediated effect on fecundity was sufficient to regulate reindeer densities around observed host densities.
Assuntos
Ostertagia/fisiologia , Rena/fisiologia , Rena/parasitologia , Reprodução , Doenças dos Animais/tratamento farmacológico , Doenças dos Animais/parasitologia , Animais , Anti-Helmínticos/uso terapêutico , Fertilidade , Interações Hospedeiro-Parasita , Ostertagia/efeitos dos fármacos , Ostertagia/isolamento & purificação , Ostertagíase/tratamento farmacológico , Ostertagíase/parasitologia , Ostertagíase/veterinária , Densidade Demográfica , Estações do Ano , Processos Estocásticos , Taxa de Sobrevida , Fatores de Tempo , Tempo (Meteorologia)RESUMO
The gastrointestinal nematode Ostertagia gruehneri is a parasite of reindeer that can have a significant impact on host population dynamics. To gain a better understanding of the population dynamics of O. gruehneri, we parameterise a model for its fecundity that describes the observed seasonal and intensity dependent pattern of faecal egg counts well. The faecal egg count model is combined with a model for the seasonal faecal production rate of Svalbard reindeer to obtain quantitative estimates of the fecundity of O. gruehneri. The model is used to evaluate the relative contribution to pasture contamination of variation in the abundance of O. gruehneri and variation in reindeer densities. It is concluded that due to the intensity dependence in nematode fecundity, variation in reindeer population densities is likely to be the most important of these factors for pasture contamination.
Assuntos
Ostertagia/fisiologia , Ostertagíase/parasitologia , Ostertagíase/veterinária , Rena/parasitologia , Animais , Fezes/parasitologia , Feminino , Fertilidade/fisiologia , Interações Hospedeiro-Parasita , Masculino , Modelos Biológicos , Contagem de Ovos de Parasitas/veterinária , Dinâmica Populacional , Fatores de TempoRESUMO
Stability of trichostrogylid populations indicates that some form of density-dependent regulation occurs which could act through fecundity. We present evidence for intraspecific density-dependent effects in 1 of 2, dominant, abomasal nematodes species (Ostertagia gruehneri) of Svalbard reindeer (Rangifer tarandus platyrhynchus). We found evidence in O. gruehneri, for density-dependent regulation of female worm length in April, July and October 1999. However, it is only in July that female worm length explains the variation in the number of eggs in utero which is also related to egg production per female worm only in this month and not at other times of the year. The seasonal pattern in faecal egg output in this species focuses egg production in the summer months when conditions are favourable to transmission. In contrast, we found no evidence in the other common species (Marshallagia marshalli) for density-dependent regulation of female worm length during or the number of eggs in utero. Faecal egg output in M. marshalli was positively related to worm burden but not to the mean number of eggs in utero. Neither inter-specific interactions nor host body condition appeared to influence worm fecundity. The contrasting patterns of density-dependent regulation of fecundity provides further evidence for divergent life-histories in this nematode community.
Assuntos
Abomaso/parasitologia , Ostertagia/fisiologia , Rena/parasitologia , Trichostrongyloidea/fisiologia , Animais , Fezes/parasitologia , Feminino , Fertilidade , Masculino , Ostertagíase/veterinária , Contagem de Ovos de Parasitas/veterinária , Dinâmica Populacional , Estações do Ano , Gastropatias/parasitologia , Gastropatias/veterinária , Trichostrongyloidea/isolamento & purificação , Tricostrongiloidíase/veterináriaRESUMO
There are only a few recent studies that have demonstrated senescence in ungulates and nothing is known regarding how patterns of senescence may vary as a function of density Senescence in general is linked to the cost of reproduction, which probably increases with density in ungulates and may differ between the sexes. Further, senescence in ungulates is also regarded to be a function of tooth wear rates. As density dependence and sexual differences in food choice have been well documented, this may lead to different tooth wear rates and, thus, possibly density-dependent and sex-specific patterns of senescence. We therefore investigated the effects of age, sex, density and their possible interactions on the variability of body weight in 29,047 red deer harvested during 1965-1998 from Norway, out of which 380 males and 1452 females were eight years or older. There was clear evidence that spatio-temporal variation in density correlated negatively with body weights. In addition, there was evidence of senescence in both male and female red deer. Age at onset of senescence in females was after 20 years of age and independent of population density. In males, the onset and rate of senescence increased with increasing population density. The onset of senescence for males was at ca. 12 years of age at low density, but decreased to approximately ten years of age at high density. The pattern of density-dependent senescence in males, but not that in females, can be explained if (i) the cost of reproduction increases with density more strongly in male than in female red deer, and/or (ii) tooth wear rates are density dependent in males, but not in females. We discuss the ability of these two different, not mutually exclusive hypotheses in explaining the observed pattern of senescence.
Assuntos
Cervos/fisiologia , Envelhecimento/fisiologia , Animais , Peso Corporal , Cervos/anatomia & histologia , Feminino , Geografia , Análise dos Mínimos Quadrados , Masculino , Modelos Biológicos , Modelos Estatísticos , Noruega , Densidade Demográfica , Caracteres SexuaisRESUMO
Large-scale climatic fluctuations, such as the North Atlantic Oscillation (NAO), have been shown to affect many ecological processes. Such effects have been typically assumed to be linear. Only one study has reported a nonlinear relation; however, that nonlinear relation was monotonic (that is, no reversal). Here we show that there is a strong nonlinear and non-monotonic (that is, reversed) effect of the NAO on body weight during the subsequent autumn for 23,838 individual wild red deer (Cervus elaphus) and 139,485 individual domestic sheep (Ovis aries) sampled over several decades on the west coast of Norway. These relationships are, at least in part, explained by comparable nonlinear and non-monotonic relations between the NAO and local climatic variables (temperature, precipitation and snow depth). The similar patterns observed for red deer and sheep, the latter of which live indoors during winter and so experience a stable energy supply in winter, suggest that the (winter) climatic variability (for which the index is a proxy) must influence the summer foraging conditions directly or indirectly.
Assuntos
Clima , Cervos , Ovinos , Animais , Animais Domésticos , Animais Selvagens , Peso Corporal , Comportamento Alimentar , Feminino , Masculino , Noruega , Estações do AnoRESUMO
The observation that the total abundance of adult nematodes in the abomasum of Svalbard reindeer increases between October and April suggests adaptation to cope with the Arctic winter. Here we investigate the extent to which selection has led to similar life-history strategies in the 3 most numerous trichostrongyle species. The life-histories are found to differ markedly. We use flexible statistical models for the abundance and dispersion of parasites in the host population. One of the taxa, Marshallagia marshalli, was most abundant and had its highest egg output in the winter. In contrast, the abundance of the most common taxa, Ostertagia gruehneri, m. gruehneri was stable or declined from autumn to late winter, and the closely related taxa, O. gruehneri, m. arcticus, showed a similar over winter drop. The faecal egg output of these 2 taxa was highest in summer, as found in temperate trichostrongyle species. Despite the apparent contamination of summer pastures with O. gruehneri, calves showed negligible burdens until their second summer and the abundance of infection reached an asymptote within their third year. In contrast, the abundance of M. marshalli in calves showed a rapid increase over the first summer and by late winter was similar to peak levels found in adults (8000 worms). This increase could not be accounted for by the developing abomasum larvae population and is therefore evidence for transmission over the winter for this taxa. While M. marshalli showed little between-year variation, O. gruehneri showed 2-fold fluctuation in the abundance of infection. O. gruehneri may therefore play a role in the fluctuating population dynamics of the host. Since there was no apparent decline in abundance with host age in any of the 3 taxa there was no evidence of reindeer mounting an immune response.
Assuntos
Abomaso/parasitologia , Modelos Biológicos , Rena/parasitologia , Trichostrongyloidea/fisiologia , Tricostrongiloidíase/veterinária , Fatores Etários , Animais , Anti-Helmínticos/uso terapêutico , Antibacterianos/uso terapêutico , Regiões Árticas/epidemiologia , Distribuição Binomial , Fezes/parasitologia , Feminino , Macrolídeos , Masculino , Noruega/epidemiologia , Ostertagia/crescimento & desenvolvimento , Ostertagia/fisiologia , Ostertagíase/epidemiologia , Ostertagíase/parasitologia , Ostertagíase/veterinária , Contagem de Ovos de Parasitas/veterinária , Dinâmica Populacional , Prevalência , Distribuição Aleatória , Estações do Ano , Trichostrongyloidea/crescimento & desenvolvimento , Tricostrongiloidíase/epidemiologia , Tricostrongiloidíase/parasitologiaRESUMO
Plant phenology of Luzula heathland plots in Spitsbergen (78°N) was manipulated by adding or removing snow, which altered the time for plots (2 m×2 m; n=10) to become snow-free. A 2-week difference in snowmelt, equivalent to approximately one-sixth of the growing season, was achieved between advanced (first to be snow-free) and delayed (last to be snow-free) treatments, which influenced plant biomass and plant quality. Nitrogen content of the forage species decreased with time after snowmelt, whereas C:N ratio increased. Manipulation of snowmelt led to a shift in "phenological time", without altering these plant quality parameters as such. Early in the growing season, Svalbard reindeer (Rangifer tarandus platyrhynchus) selected the advanced plots which had been snow-free for longest, presumably because of the greater biomass of both Luzula confusa and Salix polaris, major components of reindeer diet at that time of the year. Moreover, the proportion of live Luzula leaves was highest in advanced plots, relative to both unmanipulated control and delayed plots. In contrast, plant quality, measured as nitrogen content and C:N ratio of leaves, was lowest in the preferred plots. Phenolic content did not differ among treatments, and is therefore unlikely to play a role in reindeer selection for plots with early snowmelt. Unlike in temperate regions, where selection for plant quality seems to be of major importance, selection for plant quantity might be an outcome of generally low levels of plant biomass and high forage quality during the growing season in the high Arctic. Reindeer selection for high plant biomass is likely to lead to a more favourable nitrogen and energy return than selection for high plant quality.
RESUMO
The study aimed to compare plasma progesterone concentrations, rectal ultrasonography and plasma concentrations of pregnancy-specific protein B (PSPB) used for pregnancy diagnosis in reindeer. A total of 1,595 blood plasma samples were collected between 1991 and 1996 from 3 semidomestic reindeer (Rangifer tarandus tarandus) herds on the Norwegian mainland (Magerøy, Sørøy, Filefjell) and from 92 wild Svalbard reindeer (Rangifer tarandus platyrhynchus). Samples were collected between January and late April. Plasma levels of progesterone and PSPB were measured and used as indicators of pregnancy. In addition, animals from the Filefjell herd and the Svalbard reindeer were investigated using transrectal ultrasound. The results showed that plasma progesterone lower than 7 nmol l-1 rarely occurs in females diagnosed pregnant either by ultrasound or by observing a calf at foot 7 months after blood sampling. A very good agreement was found between plasma progesterone and PSPB when used for pregnancy diagnosis. On the Norwegian mainland, but not to the same extent on Svalbard, a high proportion of females with a high progesterone concentration was diagnosed not pregnant by ultrasound. This probably reflects a high rate of false negative diagnoses by the ultrasound method rather than false positives in the progesterone analysis.
Assuntos
Proteínas da Gravidez/sangue , Testes de Gravidez/veterinária , Prenhez/sangue , Progesterona/sangue , Rena/sangue , Útero/diagnóstico por imagem , Animais , Ensaio de Imunoadsorção Enzimática , Feminino , Noruega , Gravidez , Testes de Gravidez/métodos , Reto , Sensibilidade e Especificidade , Svalbard , UltrassonografiaRESUMO
Living in the high Arctic, the Svalbard reindeer (Rangifer tarandus platyrhynchus) and its trichostrongyle nematodes experience a long cold winter from October to late May/early June. Over this period, transmission would be expected to be low. However, in culled reindeer the abundance of infection increased from autumn to late winter, providing evidence for continued transmission within this period. To our knowledge this is the first time this has been demonstrated in a climate with temperatures consistently below 0 degrees C. In one winter (1996-1997), the average fraction of nematodes found as larvae in the abomasal mucosa increased from around 10% to 50% between October and March. This suggests that arrested development took place throughout the winter. We found no evidence for an efficient acquired immune response towards the nematodes. The abundance of infection did not tend to decrease with increasing host age after an earlier peak, but levelled off instead, as predicted by a simple immigration-death model. In the late winter when the nutritional plane is low, both adult reindeer and calves had high worm burdens at intensities that may affect their condition and fitness.
Assuntos
Infecções por Nematoides/veterinária , Rena/parasitologia , Gastropatias/veterinária , Abomaso/parasitologia , Fatores Etários , Animais , Regiões Árticas , Feminino , Larva , Masculino , Infecções por Nematoides/transmissão , Contagem de Ovos de Parasitas , Estações do Ano , Gastropatias/parasitologiaRESUMO
Sexual dimorphism results from dichotomous selection on male and female strategies of growth in relation to reproduction. In polygynous mammals, these strategies reflect sexual selection on males for access to females and competitive selection on females for access to food. Consequently, in such species, males display rapid early growth to large adult size, whereas females invest in condition and early sexual maturity at the expense of size. Hence, the magnitude of adult size dimorphism should be susceptible to divergence of the sexes in response to environmental factors differentially influencing their growth to reproduction. We show that divergent growth of male and female red deer after 32 years of winter warming and 15 years of contemporaneously earlier plant phenology support this prediction. In response to warmer climate during their early development, males grew more rapidly and increased in size, while female size declined. Conversely, females, but not males, responded to earlier plant phenology with increased investment in condition and earlier reproduction. Accordingly, adult size dimorphism increased in relation to warmer climate, whereas it declined in relation to forage quality. Thus, the evolutionary trajectories of growth related to reproduction in the sexes (i) originate from sexual and competitive selection, (ii) produce sexual size dimorphism, and (iii) are molded by environmental variation.
Assuntos
Cervos/anatomia & histologia , Cervos/fisiologia , Meio Ambiente , Ração Animal , Animais , Constituição Corporal , Peso Corporal , Clima , Feminino , Análise dos Mínimos Quadrados , Masculino , Plantas , Análise de Regressão , Reprodução , Caracteres Sexuais , Comportamento Sexual AnimalRESUMO
We present a model on plant-deer climate interactions developed for improving our understanding of the temporal dynamics of deer abundance and, in particular, how intrinsic (density-dependent) and extrinsic (plants, climate) factors influence these dynamics. The model was tested statistically by analysing the dynamics of five Norwegian red deer populations between 1964 and 1993. Direct and delayed density-dependence significantly influenced the development of the populations: delayed density-dependence primarily operated through female density, whereas direct density-dependence acted through both female and male densities. Furthermore, population dynamics of Norwegian red deer were significantly affected by climate (as measured by the global weather phenomenon, the North Atlantic Oscillation: NAO). Warm, snowy winters (high NAO) were associated with decreased deer abundance, whereas the delayed (two-year) effect of warm, snowy winters had a positive effect on deer abundance. Our analyses are argued to have profound implications for the general understanding of climate change and terrestrial ecosystem functioning.
Assuntos
Cervos , Genética Populacional , Modelos Biológicos , Modelos Teóricos , Animais , Clima , Feminino , Masculino , Noruega , Dinâmica PopulacionalRESUMO
The variability of two fitness-related phenotypic traits (body weight and a mandibular skeletal ratio) was analysed among cohorts and age-classes of red deer in Norway. Phenotypic variation among cohorts was pronounced for calves, yearlings and reproductively mature adults. Fluctuations in cohort-specific mean body weights and skeletal ratios of adults correlated with global climatic variation in winter conditions influenced by the North Atlantic Oscillation while cohorts were in utero. Red deer born following warm winters were smaller than those born after cold winters, and this inter-cohort variability persisted into adulthood. Phenotypic variation among cohorts of red deer influenced by climate change may pose consequences for fitness of cohorts since body size and condition contribute to reproductive success and survival in male and female red deer. In particular, the recent trend of increasingly warm winters in northern Europe and Scandinavia may lead to reduced body size and fecundity of red deer, and perhaps other ungulates, in those areas.
Assuntos
Clima , Cervos/anatomia & histologia , Efeito Estufa , Animais , Peso Corporal , Osso e Ossos/anatomia & histologia , Cervos/crescimento & desenvolvimento , Cervos/fisiologia , Feminino , Fertilidade , Masculino , Mandíbula/anatomia & histologia , Noruega , Fenótipo , Gravidez , Estações do AnoRESUMO
Prostaglandin PGF2 alpha seems to act satisfactorily as a birth inducing agent in red deer (Cervus elaphus). The progress of actual delivery and lactation in the experiments of induced parturition corresponds with observations from natural conditions. The pattern of response time to the birth inducing agent agrees with experience from domesticated species, being longest in females with the least developed fetuses. Complications such as those reported from practice with induced parturition in domestic species were not experienced in the three experiments on red deer. Applications of prostaglandin PGF2 alpha prior to approximately 18-20 days preterm probably reduce chances for the offspring to survive. This seemingly being due to ontogenetical immaturity in the fetus, and deficient hormonal development in the mother, necessary for normal lactogenesis.
Assuntos
Cervos/fisiologia , Trabalho de Parto/efeitos dos fármacos , Prostaglandinas F/farmacologia , Animais , Peso ao Nascer/efeitos dos fármacos , Dinoprosta , Eletrocardiografia , Feminino , Coração/efeitos dos fármacos , Frequência Cardíaca/efeitos dos fármacos , GravidezRESUMO
Radio transmitters were used to record the ECG signals of young red deer calves (Cervus elaphus) when they were exposed to various stimuli which elicited the immobility or "freezing" response. When the calves were exposed to stimuli likely to occur in the deer's natural habitat, the heart rate decreased by 25 to 85% of the pre-exposure rate. The calves displayed a more pronounced bradycardia during their first days of life than at a later age. There are also indications that repeated exposure to the same stimulus will cause a gradual waning both of the bradycardia and of the behavioural response.