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1.
Mol Biol Rep ; 51(1): 793, 2024 Jul 13.
Artigo em Inglês | MEDLINE | ID: mdl-39001985

RESUMO

BACKGROUND: Recurrent miscarriage (RM) is defined as the occurrence of at least two or three subsequent miscarriages within the 20th -24th weeks of pregnancy. The primary objective of this study was to investigate whether histidine-rich glycoprotein C633T single nucleotide polymorphism (HRG C633T SNP) statistically correlates with the occurrence of RM among Iranian women. METHODS AND RESULTS: Blood samples from 200 women were taken at the outset of the study. Then, the blood samples of 100 women who had a record of RM (case group) were compared with the other 100 women's blood samples who had no record of RM (control group). Following DNA extraction, the polymorphism of histidine-rich glycoprotein C633T (HRG C633T) for every case was specified and all women were genotyped by the amplification-refractory mutation system (ARMS) method. The results of the study revealed that there was a statistically significant difference between T/T genotype (OR = 3.5, CI (1.39-8.77), p = 0.007), and C/T genotype (OR = 1.83, CI (0.99-3.37), p = 0.05) in the case and control groups. Also, a statistically significant association was observed in T allelic frequency in the RM participants compared to the control group (OR = 2.01, CI (1.31-3.09), p = 0.01). CONCLUSIONS: The present study determined that there was a statistically significant relationship between HRG C633T SNP and increased RM regarding allelic and genotypical aspects. Moreover, it became apparent that women with homozygous T/T genotype were more susceptible to the risk of RM.


Assuntos
Aborto Habitual , Frequência do Gene , Predisposição Genética para Doença , Polimorfismo de Nucleotídeo Único , Proteínas , Adulto , Feminino , Humanos , Gravidez , Aborto Habitual/genética , Alelos , Estudos de Casos e Controles , Frequência do Gene/genética , Estudos de Associação Genética , Genótipo , Irã (Geográfico) , Polimorfismo de Nucleotídeo Único/genética , Proteínas/genética
2.
Biomed Res Int ; 2024: 2501086, 2024.
Artigo em Inglês | MEDLINE | ID: mdl-38659607

RESUMO

Purpose: Recurrent miscarriage (RM) is a significant reproductive concern affecting numerous women globally. Genetic factors are believed to play a crucial role in RM, making the histidine-rich glycoprotein (HRG) gene, a topic of interest due to its potential involvement in angiogenesis. This study is aimed at investigating the association between the HRG rs10770 genotype and RM. Method: Blood samples were collected from a total of 200 women at the beginning of the study. Subsequently, a comparative analysis was conducted between the blood samples of 100 women with a history of RM (case group) and the blood samples of another 100 healthy women (control group). HRG rs10770 genotyping was performed through polymerase chain reaction restriction-fragment length polymorphism (PCR-RFLP), followed by statistical analysis to evaluate the relationship between HRG rs10770 genotype and RM. Results: The results indicated a significant statistical difference between the C/C genotype (OR = 3.32, CI: 1.22-9.04, p = 0.01) and the C/T genotype (OR = 1.24, CI: 0.67-2.30, p = 0.47) in both the case and control groups. Additionally, a significant correlation was observed in the C allelic frequency among RM participants compared to the control group (OR = 1.65, CI: 1.06-2.58, p = 0.02). Conclusion: The study highlights the importance of HRG rs10770 in understanding RM, shedding light on its implications for reproductive health. Furthermore, it became evident that women carrying the homozygous C/C genotype exhibited increased susceptibility to the risk of RM.


Assuntos
Aborto Habitual , Predisposição Genética para Doença , Adulto , Feminino , Humanos , Gravidez , Aborto Habitual/genética , Alelos , Estudos de Casos e Controles , Estudos de Associação Genética , Genótipo , Irã (Geográfico) , Polimorfismo de Nucleotídeo Único/genética , Proteínas/genética
3.
Biomed Res Int ; 2021: 3422138, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34778450

RESUMO

PURPOSE: Recurrent miscarriage applies to pregnancy loss expulsion of the fetus within the first 24 weeks of pregnancy. This study is aimed at comparatively investigating the sera of women with RM with those who have no record of miscarriages to identify if there were any metabolite and metabolic pathway differences using 1H NMR spectroscopy. METHODS: Serum samples were collected from women with RM (n = 30) and those who had no records of RM (n = 30) to obtain metabolomics information. 1H NMR spectroscopy was carried out on the samples using Carr Purcell Meiboom Gill spin echo; also, Partial Least Squares Discriminant Analysis was performed in MATLAB software using the ProMetab program to obtain the classifying chemical shifts; the metabolites were identified by using the Human Metabolome Database (HMDB) in both the experimental and control groups. The pathway analysis option of the Metaboanalyst.ca website was used to identify the changed metabolic pathways. RESULTS: The results of the study revealed that 14 metabolites were different in the patients with RM. Moreover, the pathway analysis showed that taurine and hypotaurine metabolism along with phenylalanine, tyrosine, and tryptophan biosynthesis was significantly different in patients with RM. CONCLUSION: The present study proposes that any alteration in the above metabolic pathways might lead to metabolic dysfunctions which may result in a higher probability of RM.


Assuntos
Aborto Habitual/metabolismo , Gravidez/metabolismo , Aborto Habitual/sangue , Adulto , Análise Discriminante , Feminino , Humanos , Irã (Geográfico)/epidemiologia , Análise dos Mínimos Quadrados , Espectroscopia de Ressonância Magnética/métodos , Redes e Vias Metabólicas/fisiologia , Metaboloma , Metabolômica/métodos , Espectroscopia de Prótons por Ressonância Magnética , Soro/química , Soro/metabolismo
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