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Cellulosic biomass-based sustainable aviation fuels (SAFs) can be produced from various feedstocks. The breakeven price and carbon intensity of these feedstock-to-SAF pathways are likely to differ across feedstocks and across spatial locations due to differences in feedstock attributes, productivity, opportunity costs of land for feedstock production, soil carbon effects, and feedstock composition. We integrate feedstock to fuel supply chain economics and life-cycle carbon accounting using the same system boundary to quantify and compare the spatially varying greenhouse gas (GHG) intensities and costs of GHG abatement with SAFs derived from four feedstocks (switchgrass, miscanthus, energy sorghum, and corn stover) at 4 km resolution across the U.S. rainfed region. We show that the optimal feedstock for each location differs depending on whether the incentive is to lower breakeven price, carbon intensity, or cost of carbon abatement with biomass or to have high biomass production per unit land. The cost of abating GHG emissions with SAF ranges from $181 Mg-1 CO2e to more than $444 Mg-1 CO2e and is lowest with miscanthus in the Midwest, switchgrass in the south, and energy sorghum in a relatively small region in the Great Plains. While corn stover-based SAF has the lowest breakeven price per gallon, it has the highest cost of abatement due to its relatively high GHG intensity. Our findings imply that different types of policies, such as volumetric targets, tax credits, and low carbon fuel standards, will differ in the mix of feedstocks they incentivize and locations where they are produced in the U.S. rainfed region.
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Biomassa , Gases de Efeito Estufa , Celulose , Efeito Estufa , Biocombustíveis , AviaçãoRESUMO
Metal halide perovskite solar cells (PSCs) are infamous for their batch-to-batch and lab-to-lab irreproducibility in terms of stability and performance. Reproducible fabrication of PSCs is a critical requirement for market viability and practical commercialization. PSC irreproducibility plagues all levels of the community; from institutional research laboratories, start-up companies, to large established corporations. In this work, the critical function of atmospheric humidity to regulate the crystallization and stabilization of formamidinium lead triiodide (FAPbI3) perovskites is unraveled. It is demonstrated that the humidity content during processing induces profound variations in perovskite stoichiometry, thermodynamic stability, and optoelectronic quality. Almost counterintuitively, it is shown that the presence of humidity is perhaps indispensable to reproduce phase-stable and efficient FAPbI3-based PSCs.
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The importance of light management for perovskite solar cells (PSCs) has recently been emphasized because their power conversion efficiency approaches their theoretical thermodynamic limits. Among optical strategies, anti-reflection (AR) coating is the most widely used method to reduce reflectance loss and thus increase light-harvesting efficiency. Monolayer MgF2is a well-known AR material because of its optimal refractive index, simple fabrication process, and physical and chemical durabilities. Nevertheless, quantitative estimates of the improvement achieved by the MgF2AR layer are lacking. In this study, we conducted theoretical and experimental evaluations to assess the AR effect of MgF2on the performance of formamidinium lead-triiodide PSCs. A sinusoidal tendency to enhance the short-circuit current density (JSC) was observed depending on the thickness, which was attributed to the interference of the incident light. A transfer matrix method-based simulation was conducted to calculate the optical losses, demonstrating the critical impact of reflectance loss on theJSCimprovement. The predictedJSCs values, depending on the perovskite thickness and the incident angle, are also presented. The combined use of experimental and theoretical approaches offers notable advantages, including accurate interpretation of photocurrent generation, detailed optical analysis of the experimental results, and device performance predictions under unexplored conditions.
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The deleterious effects of ozone (O3) pollution on crop physiology, yield, and productivity are widely acknowledged. It has also been assumed that C4 crops with a carbon concentrating mechanism and greater water use efficiency are less sensitive to O3 pollution than C3 crops. This assumption has not been widely tested. Therefore, we compiled 46 journal articles and unpublished datasets that reported leaf photosynthetic and biochemical traits, plant biomass, and yield in five C3 crops (chickpea, rice, snap bean, soybean, and wheat) and four C4 crops (sorghum, maize, Miscanthus × giganteus, and switchgrass) grown under ambient and elevated O3 concentration ([O3]) in the field at free-air O3 concentration enrichment (O3-FACE) facilities over the past 20 y. When normalized by O3 exposure, C3 and C4 crops showed a similar response of leaf photosynthesis, but the reduction in chlorophyll content, fluorescence, and yield was greater in C3 crops compared with C4 crops. Additionally, inbred and hybrid lines of rice and maize showed different sensitivities to O3 exposure. This study quantitatively demonstrates that C4 crops respond less to elevated [O3] than C3 crops. This understanding could help maintain cropland productivity in an increasingly polluted atmosphere.
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Oryza , Ozônio , Fotossíntese/fisiologia , Clorofila , Folhas de Planta/fisiologia , Poaceae , Zea mays/fisiologia , Produtos Agrícolas/genética , Oryza/genética , Dióxido de Carbono/farmacologiaRESUMO
There are few peer-reviewed studies documenting saturated buffer annual nitrate (NO3 ) removal or that have assessed the federal practice standard design criteria. Drainage flow, NO3 , and dissolved reactive phosphorus (DRP) were monitored at three saturated buffers in Illinois, USA, for a combined 10 site-years. Nitrate loss reduction averaged 48 ± 19% with removals of 3.5-25.2 kg NO3 -N ha-1 annually. Median DRP concentrations at all sampling locations were at the analytical detection limit of 0.01 mg L-1 . The current design paradigm (i.e., USDA practice standard) prescribes there should be no flow bypassing the saturated buffer at flow rates that are ≤5% of the peak drainage system flow rate. The drainage coefficient-based and Manning's equation-based peak flow estimates were higher and lower, respectively, than the observed annual peaks in all years. This illustrated inherent uncertainty introduced early in the design process, which can be further compounded by dynamic in-buffer hydrology. The percentage of the observed peak flow rate at which bypass initiated ranged across an order of magnitude between sites (4.4-8.1% of peak flow rate at one site and 42-49% of peak at another) despite the buffers providing relatively similar NO3 removal. Bypass at one site (SB2) was related to the concept of "antecedent buffer capacity filled," which was defined as the 5-d average water depth in the middle control structure chamber expressed as a relative percentage of the bypass stop log height. This design flow analysis serves as a call to further evaluate predictive relationships and design models for edge-of-field practices.
Core Ideas Three saturated buffers in Illinois provided an ≈50% annual reduction in NO3 load. Observed peak flow rates differed from estimation methods used for design purposes. Two sites had relatively similar nitrate removals but different bypass trends. "Antecedent buffer capacity filled" was the water depth in the middle chamber as a percent of stop log height.
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Monitoramento Ambiental , Nitratos , Agricultura , Hidrologia , Illinois , Nitratos/análise , Nitrogênio/análise , Fósforo/análiseRESUMO
Optoelectronic devices consist of heterointerfaces formed between dissimilar semiconducting materials. The relative energy-level alignment between contacting semiconductors determinately affects the heterointerface charge injection and extraction dynamics. For perovskite solar cells (PSCs), the heterointerface between the top perovskite surface and a charge-transporting material is often treated for defect passivation1-4 to improve the PSC stability and performance. However, such surface treatments can also affect the heterointerface energetics1. Here we show that surface treatments may induce a negative work function shift (that is, more n-type), which activates halide migration to aggravate PSC instability. Therefore, despite the beneficial effects of surface passivation, this detrimental side effect limits the maximum stability improvement attainable for PSCs treated in this way. This trade-off between the beneficial and detrimental effects should guide further work on improving PSC stability via surface treatments.
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We conducted a retrospective cohort study using claims data to determine the number and types of complications from coronavirus disease (COVID-19) that patients experience and which patients are more vulnerable to those complications compared with complications in patients with influenza. Among the cohort, 19.6% of COVID-19 patients and 28.5% of influenza patients had >1 new complication. In most complications, COVID-19 patients had lower or similar relative risk compared with influenza patients; exceptions were hair loss, heart failure, mood disorder, and dementia. Young to middle-aged adult COVID-19 patients and patients in COVID-19 hotspots had a higher risk for complications. Overall, COVID-19 patients had fewer complications than influenza patients, but caution is necessary in high-risk groups. If the fatality rate for COVID-19 is reduced through vaccination, management strategies for this disease could be adapted, similar to those for influenza management, such as easing restrictions on economic activity or requirements for close-contact isolation.
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COVID-19 , Influenza Humana , Adulto , Humanos , Influenza Humana/complicações , Influenza Humana/epidemiologia , Pessoa de Meia-Idade , Estudos Retrospectivos , SARS-CoV-2 , Estações do AnoRESUMO
BACKGROUND: In the era of coronavirus disease 2019 (COVID-19) pandemic, there is a paucity of information regarding actual prevalence of COVID-19 in pregnant women compared to non-pregnant women. The purpose of this study was to investigate the prevalence of COVID-19 infection and clinical outcome in pregnant women and non-pregnant women. METHODS: This is a nationwide cross-sectional study in South Korea between January 2020 and February 2021 using the claim database. The primary outcome was the prevalence of COVID-19 in pregnant women, and the secondary outcome was the occurrence of severe COVID-19 illness among infected patients. Severity of COVID-19 was classified into four categories according to WHO ordinal scale. RESULTS: The prevalence of COVID-19 infection was lower in pregnant women than non-pregnant women aged 20-44 (0·02% vs. 0.14%, p < 0.0001). However, among COVID-19 positive women at age 20-44, pregnant women was at higher risk of oxygen therapy after hospitalization (score 4 in WHO ordinal scale: 6.4% vs. 1.6%, p < 0.05). There were no deaths or hospitalized severe disease in pregnant women with COVID-19, although the majority of them (96·2%) were admitted to hospital. On the other hand, 42·3% of non-pregnant women at 20-44 age were admitted to hospital and 0.04% of them died and 0.1% had hospitalized severe disease. CONCLUSIONS: The prevalence of COVID-19 infection in pregnant women was lower than non-pregnant women in Korea, resulting in relatively small cases of fatality. It has implications that public health policy, such as an effective response to COVID-19 and a powerful preemptive strategy for pregnant women, can lower risk of COVID-19 infection and better clinical outcomes in pregnant women with COVID-19.
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COVID-19 , Complicações Infecciosas na Gravidez , Adulto , Estudos Transversais , Feminino , Humanos , Gravidez , Complicações Infecciosas na Gravidez/epidemiologia , Resultado da Gravidez/epidemiologia , Gestantes , Prevalência , SARS-CoV-2 , Adulto JovemRESUMO
There is tremendous interspecific variability in O3 sensitivity among C3 species, but variation among C4 species has been less clearly documented. It is also unclear whether stomatal conductance and leaf structure such as leaf mass per area (LMA) determine the variation in sensitivity to O3 across species. In this study, we investigated leaf morphological, chemical, and photosynthetic responses of 22 genotypes of four C4 bioenergy species (switchgrass, sorghum, maize, and miscanthus) to elevated O3 in side-by-side field experiments using free-air O3 concentration enrichment (FACE). The C4 species varied largely in leaf morphology, physiology, and nutrient composition. Elevated O3 did not alter leaf morphology, nutrient content, stomatal conductance, chlorophyll fluorescence, and respiration in most genotypes but reduced net CO2 assimilation in maize and photosynthetic capacity in sorghum and maize. Species with lower LMA and higher stomatal conductance tended to show greater losses in photosynthetic rate and capacity in elevated O3 compared with species with higher LMA and lower stomatal conductance. Stomatal conductance was the strongest determinant of leaf photosynthetic rate and capacity. The response of both area- and mass-based leaf photosynthetic rate and capacity to elevated O3 were not affected by LMA directly but negatively influenced by LMA indirectly through stomatal conductance. These results demonstrate that there is significant variation in O3 sensitivity among C4 species with maize and sorghum showing greater sensitivity of photosynthesis to O3 than switchgrass and miscanthus. Interspecific variation in O3 sensitivity was determined by direct effects of stomatal conductance and indirect effects of LMA. This is the first study to provide a test of unifying theories explaining variation in O3 sensitivity in C4 bioenergy grasses. These findings advance understanding of O3 tolerance in C4 grasses and could aid in optimal placement of diverse C4 bioenergy feedstock across a polluted landscape.
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Ozônio , Fotossíntese/fisiologia , Folhas de Planta/fisiologia , Zea mays/genéticaRESUMO
Prairie cordgrass (Spartina pectinata Link) is a native perennial warm-season (C4) grass common in North American prairies. With its high biomass yield and abiotic stress tolerance, there is a high potential of developing prairie cordgrass for conservation practices and as a dedicated bioenergy crop for sustainable cellulosic biofuel production. However, as with many other undomesticated grass species, little information is known about the genetic diversity or population structure of prairie cordgrass natural populations as compared to their ecotypic and geographic adaptation in North America. In this study, we sampled and characterized a total of 96 prairie cordgrass natural populations with 9315 high quality SNPs from a genotyping-by-sequencing (GBS) approach. The natural populations were collected from putative remnant prairie sites throughout the Midwest and Eastern USA, which are the major habitats for prairie cordgrass. Partitioning of genetic variance using SNP marker data revealed significant variance among and within populations. Two potential gene pools were identified as being associated with ploidy levels, geographical separation, and climatic separation. Geographical factors such as longitude and altitude, and environmental factors such as annual temperature, annual precipitation, temperature of the warmest month, precipitation of the wettest month, precipitation of Spring, and precipitation of the wettest month are important in affecting the intraspecific distribution of prairie cordgrass. The divergence of prairie cordgrass natural populations also provides opportunities to increase breeding value of prairie cordgrass as a bioenergy and conservation crop.
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Ecossistema , Variação Genética , Genoma de Planta , Poaceae/genética , Filogeografia , Poaceae/classificação , Estados UnidosRESUMO
Elevated tropospheric ozone concentration (O3 ) significantly reduces photosynthesis and productivity in several C4 crops including maize, switchgrass and sugarcane. However, it is unknown how O3 affects plant growth, development and productivity in sorghum (Sorghum bicolor L.), an emerging C4 bioenergy crop. Here, we investigated the effects of elevated O3 on photosynthesis, biomass and nutrient composition of a number of sorghum genotypes over two seasons in the field using free-air concentration enrichment (FACE), and in growth chambers. We also tested if elevated O3 altered the relationship between stomatal conductance and environmental conditions using two common stomatal conductance models. Sorghum genotypes showed significant variability in plant functional traits, including photosynthetic capacity, leaf N content and specific leaf area, but responded similarly to O3 . At the FACE experiment, elevated O3 did not alter net CO2 assimilation (A), stomatal conductance (gs ), stomatal sensitivity to the environment, chlorophyll fluorescence and plant biomass, but led to reductions in the maximum carboxylation capacity of phosphoenolpyruvate and increased stomatal limitation to A in both years. These findings suggest that bioenergy sorghum is tolerant to O3 and could be used to enhance biomass productivity in O3 polluted regions.
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Ozônio/farmacologia , Fotossíntese/efeitos dos fármacos , Sorghum/metabolismo , Clorofila/metabolismo , Folhas de Planta/metabolismo , Folhas de Planta/fisiologia , Transpiração Vegetal/efeitos dos fármacos , Sorghum/efeitos dos fármacos , Sorghum/crescimento & desenvolvimento , Sorghum/fisiologiaRESUMO
Additive engineering is known to be an effective method for inducing a simultaneous effect of enlarging the grain size and surface passivation. As compared to the monovalent halides frequently used as additives, divalent halides are relatively less investigated in the role of additives. In this work, we report effects of alkaline earth metal halides BCl2 (B = Mg, Ca, Sr, Ba) as additives on the opto-electronic properties and photovoltaic performance of FAPbI3 based perovskite solar cells (PSCs). A significant improvement in power conversion efficiency (PCE) from 17.27% to 21.11% is observed by MgCl2 addition in the FAPbI3 precursor solution, while a marginal increment for CaCl2 or BaCl2 and a negative effect for SrCl2 is observed. The lattice constant of cubic FAPbI3 is hardly changed by additives, while the crystallinity is improved by MgCl2. The carrier lifetime increases from 40 ns to 287 ns and the trap density is reduced from 1.08 × 1016 cm-3 to 3.19 × 1015 cm-3 by addition of 5 mol% MgCl2, which is responsible for the enhancement in photovoltaic parameters. The steady-state PCE of the PSC with the MgCl2-additive-treated FAPbI3 measured under continuous illumination at the maximum power point remains unchanged for 1500 s.
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Lead halide perovskite (APbX3) has recently emerged as a promising active layer in light-emitting diodes (LEDs) as well as an absorber for photovoltaic devices. For better LED properties, it is important to understand the fundamental mechanism of the optoelectronic behaviors, e.g., how the nanostructure of the APbX3 thin film correlates with its emitting properties. We investigated the effect of APbBr3 (A = CH3NH3, Cs) crystallite size on the photophysical properties regarding its crystallographic changes and spin-orbit coupling. Photoluminescence lifetime measurements, X-ray and electron diffraction analyses, and density functional theory calculations were performed. We demonstrate that the emitting properties of mesoscale APbBr3 crystallites are improved due to the formation of a pure cubic phase that leads to the spin- and momentum-allowed carrier recombination. Our findings provide fundamental insights into the emitting behavior of APbBr3, which suggests a control of its optoelectronic properties by means of modulating the crystal morphology and resultant electronic band structures.
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PURPOSE: The aim of this study is to assess the accuracy of alignment determined by patient-specific instrumentation system in total knee arthroplasty(TKA). MATERIALS AND METHODS: Twenty-seven TKAs using patient-specific instrument were reviewed. The intraoperative pin location determined by the patient-specific guide was recorded using imageless navigation software. Data recorded included tibial coronal alignment and posterior slope, femoral coronal alignment and sagittal alignment, and transepicondylar axis. A discrepancy within ±3° in each plane was considered an acceptable result. RESULTS: On the tibia, an acceptable alignment was obtained in 24 (88.1%) in the coronal plane and 21 (77.8%) in the sagittal plane. On the femur, a satisfactory alignment was obtained in 25 (92.6%) in the coronal plane and 24 (88.1%) in the sagittal plane. Based on the transepicondylar axis, a satisfactory alignment was obtained in 23 (85.1%). CONCLUSIONS: Satisfactory alignment was obtained in more than 85% of each plane of the femur and in the coronal plane of the tibia and relative to the transepicondylar axis. Sufficeint experience and precise preoperative planning are required to improve the accuracy of sagittal alignment of the tibia.
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High-efficiency perovskite solar cells are generally fabricated by using highly pure (>99.99 %) PbI2 mixed with an organic iodide in polar aprotic solvents. However, the use of such an expensive chemical may impede progress toward large-scale industrial applications. Here, we report on the synthesis of perovskite powders by using inexpensive low-grade (99 %) PbI2 and on the photovoltaic performance of perovskite solar cells prepared from a powder-based single precursor. Pure APbI3 [A=methylammonium (MA) or formamidinium (FA)] perovskite powders were synthesized by treating low-grade PbI2 with MAI or FAI in acetonitrile at ambient temperature. The structural phase purity was confirmed by X-ray diffraction. The solar cell with a MAPbI3 film prepared from the synthesized perovskite powder demonstrated a power conversion efficiency (PCE) of 17.14 %, which is higher than the PCE of MAPbI3 films prepared by using both MAI and PbI2 as precursors (PCE=13.09 % for 99 % pure PbI2 and PCE=16.39 % for 99.9985 % pure PbI2 ). The synthesized powder showed better absorption and photoluminescence, which were responsible for the better photovoltaic performance. For the FAPbI3 powder, a solution with a yellow non-perovskite δ-FAPbI3 powder synthesized at room temperature was found to lead to a black perovskite film, whereas a solution with the black perovskite α-FAPbI3 powder synthesized at 150 °C was not transformed into a black perovskite film. The αâδ transition between the powder and film was assumed to correlate with the difference in the iodoplumbates in the powder-dissolved solution. An average PCE of 17.21 % along with a smaller hysteresis [ΔPCE=PCEreverse -PCEforward )=1.53 %] was demonstrated from the perovskite solar cell prepared by using δ-FAPbI3 powder; this PCE is higher than the average PCE of 17.05 % with a larger hysteresis (ΔPCE=2.71 %) for a device based on a conventional precursor solution dissolving MAI with high-purity PbI2 . The smaller hysteresis was indicative of fewer defects in the resulting FAPbI3 film prepared by using the δ-FAPbI3 powder.
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We report on the synthesis of porous silica particles by self-assembly routes in a continuous manner for application to thermal insulators. A continuous process was employed to produce tiny droplets containing precursor materials such as silica and organic templates for self-organization to fabricate particles with well defined pores. A rotating cylinder system or a spray drying process was adopted to form emulsions or aerosol droplets as micro-reactors for self-assembly, and the physical properties including the thermal conductivity of the resulting porous particles were compared between the two methods. The porous particles could be coated as a thick film by solution dripping, and the fluorination treatment using a silane coupling agent was performed to produce superhydrophobic surfaces of insulating layers by a lotus effect.
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Anomalous current-voltage (J-V) hysteresis in perovskite (PSK) solar cell is open to dispute, where hysteresis is argued to be due to electrode polarization, dipolar polarization, and/or native defects. However, a correlation between those factors and J-V hysteresis is hard to be directly evaluated because they usually coexist and are significantly varied depending on morphology and crystallinity of the PSK layer, selective contacts, and device architecture. In this study, without changing morphology and crystallinity of PSK layer in a planar heterojunction structure employing FA0.9Cs0.1PbI3, a correlation between J-V hysteresis and trap density is directly evaluated by means of thermally induced PbI2 regulating trap density. Increase in thermal annealing time at a given temperature of 150 °C induces growth of PbI2 on the PSK grain surface, which results in significant reduction of nonradiative recombination. Hysteresis index is reduced from 0.384 to 0.146 as the annealing time is increased from 5 to 100 min due to decrease in the amplitude of trap-mediated recombination. Reduction of hysteresis by minimizing trap density via controlling thermal annealing time leads to the stabilized PCE of 18.84% from the normal planar structured FA0.9Cs0.1PbI3 PSK solar cell.
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The complete chloroplast (cp) genomes of three Echinochloa crus-galli accessions (KR822684, KR822685, and KR822686) are reported in this work. The cp genome size is similar in three accessions, ranging from 139 846 bp to 139 860 bp. All three genomes have two inverted repeats (IR) of 22 748 bp per each IR with a large single copy (LSC) region of 81 833-81 844 bp and a small single copy (SSC) region of 12 517-12 520 bp. The total of 131 genes was identified in individual accession. Phylogenetic analysis revealed three Korean Echinochloa accessions belonged to E. crus-galli, and diverged less than 0.1 million years ago (Mya).
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Echinochloa/genética , Genoma de Cloroplastos/genética , Genoma Mitocondrial/genética , Composição de Bases/genética , Sequência de Bases/genética , Evolução Biológica , Cloroplastos/genética , DNA de Cloroplastos/genética , Ordem dos Genes , Genes Mitocondriais/genética , Genes de Plantas , Genoma/genética , Genoma de Planta/genética , Mitocôndrias/genética , Filogenia , Análise de Sequência de DNA/métodosRESUMO
Soil samples were collected on farmland in a lead-zinc mining area in the Karst region of Guangxi, China. The contamination of the soil by eight metals (Cd, Hg, As, Cu, Pb, Cr, Zn, Ni) was determined. Among all these metals, Cd is the most serious pollutant in this area. Zn, Hg as well asPb can also be measured at high levels, which may affect the crop production. All other metals contributed marginally to the overall soil contamination. Besides the evaluation of single metals, the Nemerow synthetic index indicated that the soil is not suitable for agricultural use.
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Chumbo , Metais Pesados/análise , Mineração , Poluentes do Solo/análise , Zinco , China , Chumbo/análise , Zinco/análiseRESUMO
PURPOSE: Bioreducible crosslinked polyplexes were prepared via disulfide bond formation after siRNA condensation with polyethylenimine-modified by deoxycholic acid (PEI-DA) to stabilize polyplex structure in an extracellular environment and to promote transfection efficiency in human smooth muscle cells (hSMCs). METHODS: The PEI-DA/siRNA polyplexes were further modified by crosslinking the primary amines of PEI with thiol-cleavable crosslinkers. The effect of disulfide crosslinked PEI-DA/siRNA (Cr PEI-DA/siRNA) polyplexes on target gene silencing was investigated by transfecting hSMCs with matrix metalloproteinase-2 (MMP-2) siRNA under serum conditions. The MMP-2 levels in the conditioned medium were examined using gelatin zymography. RESULTS: The Cr PEI-DA/siRNA polyplexes showed increased stability against heparin exchange reactions, while their disulfide linkages were successfully cleaved under reducing conditions. The polyplex crosslinking reaction led to a slight decrease in MMP-2 gene silencing activity in hSMCs due to the insufficient redox potential. However, the gene silencing efficiency of the Cr PEI-DA/siRNA polypexes was gradually improved in response to increasing intracellular reduction potential. The increased serum stability of the Cr PEI-DA/siRNA polyplexes resulted in significant enhancement of the intracellular delivery efficiency especially under serum conditions. CONCLUSION: The Cr PEI-DA/siRNA polyplex formulation may be a promising siRNA delivery system for the treatment of incurable genetic disorders.