PSMD1 as a prognostic marker and potential target in oropharyngeal cancer.

BACKGROUND: Despite the diverse genetic mutations in head and neck cancer, the chemotherapy outcome for this cancer has not improved for decades. It is urgent to select prognostic factors and therapeutic targets for oropharyngeal cancer to establish precision medicine. Recent studies have identified PSMD1 as a potential prognostic marker in several cancers. We aimed to assess the prognostic significance of PSMD1 expression in oropharyngeal squamous cell carcinoma (OPSCC) patients using immunohistochemistry. METHODS: We studied 64 individuals with OPSCC tissue from surgery at Seoul National University Bundang Hospital between April 2008 and August 2017. Immunostaining analysis was conducted on the tissue microarray (TMA) sections (4 µm) for p16 and PSMD1. H-score, which scale from 0 to 300, was calculated from each nucleus, cytoplasm, and cellular expression. Clinicopathological data were compared with Chi-squared test, Fisher's exact test, t-test, and logistic regression. Survival data until 2021 were achieved from national statistical office of Korea. Kaplan-Meier method and cox-regression model were used for disease-specific survival (DSS) analysis. RESULTS: H-score of 90 in nucleus was appropriate cutoff value for 'High PSMD1 expression' in OPSCC. Tonsil was more frequent location in low PSMD1 group (42/52, 80.8%) than in high PSMD1 group (4/12, 33.3%; P = .002). Early-stage tumor was more frequent in in low PSMD1 group (45/52, 86.5%) than in high PSMD1 group (6/12, 50%; P = .005). HPV was more positive in low PSMD1 group (43/52, 82.7%) than in high PSMD1 group (5/12, 41.7%; P = .016). Patients with PSMD1 high expression showed poorer DSS than in patients with PSMD1 low expression (P = .006 in log rank test). In multivariate analysis, PSMD1 expression, pathologic T staging, and specimen age were found to be associated with DSS (P = .011, P = .025, P = .029, respectively). CONCLUSIONS: In our study, we established PSMD1 as a negative prognostic factor in oropharyngeal squamous cell carcinoma, indicating its potential as a target for targeted therapy and paving the way for future in vitro studies on drug repositioning.

Ultrasonic testing of rivet in multilayer structure using a convolutional neural network on edge device.

Rivets are used to assemble layers in the air intakes, fuselages, and wings of an aircraft. After a long time of working under extreme conditions, pitting corrosion could appear in the rivets of the aircraft. The rivets could be broken down and thread the safety of the aircraft. In this paper, we proposed an ultrasonic testing method integrated with convolutional neural network (CNN) for the detection of corrosion in the rivets. The CNN model was designed to be lightweight enough to be able to run on edge devices. The CNN model was trained with a very limited sample of rivets, from 3 to 9 artificial pitting corrosive rivets. The results show that the proposed approach could detect up to 95.2% of pitting corrosion using experimental data with three training rivets. The detection accuracy could be improved to 99% by nine training rivets. The CNN model was implemented and ran on an edge device (Jetson Nano) in real-time with a small latency of 1.65 ms.

Characteristic Analysis of Homo- and Heterodimeric Complexes of Human Mitochondrial Pyruvate Carrier Related to Metabolic Diseases.

Human mitochondrial pyruvate carriers (hMPCs), which are required for the uptake of pyruvate into mitochondria, are associated with several metabolic diseases, including type 2 diabetes and various cancers. Yeast MPC was recently demonstrated to form a functional unit of heterodimers. However, human MPC-1 (hMPC-1) and MPC-2 (hMPC-2) have not yet been individually isolated for their detailed characterization, in particular in terms of their structural and functional properties, namely, whether they exist as homo- or heterodimers. In this study, hMPC-1 and hMPC-2 were successfully isolated in micelles and they formed stable homodimers. However, the heterodimer state was found to be dominant when both hMPC-1 and hMPC-2 were present. In addition, as heterodimers, the molecules exhibited a higher binding capacity to both substrates and inhibitors, together with a larger structural stability than when they existed as homodimers. Taken together, our results demonstrated that the hetero-dimerization of hMPCs is the main functional unit of the pyruvate metabolism, providing a structural insight into the transport mechanisms of hMPCs.

Two-Dimensional Solar Wind Speeds from 6 to 26 Solar Radii in Solar Cycle 24 by Using Fourier Filtering.

Measurement of the solar wind speed near the Sun is important for understanding the acceleration mechanism of the solar wind. In this Letter, we determine 2D solar wind speeds from 6 to 26 solar radii by applying Fourier motion filters to SOHO/LASCO C3 movies observed from 1999 to 2010. Our method successfully reproduces the original flow speeds in the artificially generated data as well as streamer blobs. We measure 2D solar wind speeds from one-day to one-year timescales and their variation in solar cycle 24. We find that the solar wind speeds at timescales longer than a month in the solar maximum period are relatively uniform in the azimuthal direction, while they are clearly bimodal in the minimum period, as expected from the Ulysses observations and interplanetary radio scintillation reconstruction. The bimodal structure appears at around 2006, becomes most distinctive in 2009, and abruptly disappears in 2010. The radial evolution of the solar wind speeds resembles the Parker's solar wind solution.

Prevalence and associated factors of dysphagia in nursing home residents.

Dysphagia is a major health care problem in nursing homes. It can lead to fatal complications including aspiration pneumonia and even death. The aims of this study were to evaluate the prevalence of dysphagia in nursing home residents in South Korea and to identify factors associated with dysphagia. The study was conducted in two urban nursing homes and a total of 395 older adults (aged 65-103 years old, 76.5% female) were enrolled. The presence of dysphagia was evaluated using the Gugging Swallowing Screen (GUSS) test. Out of 395 older adults, the prevalence of dysphagia was 52.7%. The following were all risk factors associated with dysphagia: Aged 75 years or older, male gender, history of dementia, feeding time longer than 20 min, severe dependent functional status, solid meal type, high nutritional risk, and underweight. To prevent complications from dysphagia in nursing home residents, systematic screening and evaluation programs for dysphagia are needed.