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Wastewater discharge from wastewater treatment plants continuously pumps microplastics into rivers, yet their transport distances within these waterways remain unknown. Herein, we developed a conceptual framework by synthesizing the microplastic data from the Yangtze River Basin to evaluate its transport distances, quantifying a significant spatial dependence between large-scale wastewater discharge and riverine microplastics (p < 0.05). The presence of microplastics at a specific sampling site could be attributed to wastewater discharge within a large-scale range spanning >1000 km upstream, encompassing a substantial portion equivalent to one-third of the Yangtze River Basin. The dominance analysis indicated that the contribution of wastewater discharge in rivers with higher discharge (>100 m3/s) to riverine microplastic pollution exceeded 65% within the Yangtze River Basin. The spatial dependence framework of riverine microplastics on wastewater discharge advances our prior understanding of the prevention and control of riverine microplastics by demonstrating that such pollution is not limited to nearby environmental factors.
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Plastics in aquatic ecosystems rapidly undergo biofouling, giving rise to a new ecosystem on their surface, the 'plastisphere.' Few studies quantify the impact of plastics and their associated community on ecosystem traits from biodiversity and functional traits to metabolic function. It has been suspected that impacts on ecosystems may depend on its state but comparative studies of ecosystem responses are rare in the published literature. We quantified algal biomass, bacterial and algal biodiversity (16S and 18S rRNA), and metabolic traits of the community growing on the surface of different plastic polymers incubated within rivers of the Lower Mekong Basin. The rivers selected have different ecological characteristics but are similar regarding their high degree of plastic pollution. We examined the effects of plastics colonized with biofilms on ecosystem production, community dark respiration, and the epiplastic community's capability to influence nitrogen, phosphorus, organic carbon, and oxygen in water. Finally, we present conceptual models to guide our understanding of plastic pollution within freshwaters. Our findings showed limited microalgal biomass and bacterial dominance, with potential pathogens present. The location significantly influenced community composition, highlighting the role of environmental conditions in shaping community development. When assessing the effects on ecosystem productivity, our experiments showed that biofouled plastics led to a significant drop in oxygen concentration within river water, leading to hypoxic/anoxic conditions with subsequent profound impacts on system metabolism and the capability of influencing biogeochemical cycles. Scaling our findings revealed that plastic pollution may exert a more substantial and ecosystem-altering impact than initially assumed, particularly in areas with poorly managed plastic waste. These results highlighted that the plastisphere functions as a habitat for biologically active organisms which play a pivotal role in essential ecosystem processes. This warrants dedicated attention and investigation, particularly in sensitive ecosystems like the Mekong River, which supports a rich biodiversity and the livelihoods of 65 million people.
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Ecossistema , Plásticos , Rios , Rios/microbiologia , Biodiversidade , Biomassa , Poluentes Químicos da ÁguaRESUMO
Textile fibre evidence can provide important activity level information in criminal cases. To date, very few studies have investigated fibre persistence on fabrics exposed to aquatic conditions, even though items of evidence and victim's bodies can regularly be found in aquatic environments. This lack of research on whether fibres (and other trace evidence) persist on evidence submerged in water, has shown to impact practice as it is reported that crime scene examiners do not attempt to recover this evidence, due to the belief that it would not be present. The dynamic nature of aquatic environments mean that the studies are difficult to conduct in situ and variables, such as water flow rate are not possible to control and thought to be difficult to monitor. To address these challenges, artificial streams (also known as mesocosms) were employed in this study to investigate the persistence rate of polyester fibres on different fabric types (Woollen/nylon mix carpet, 100% polyester fleece, and 95% polyester/5% elastane sports vest) for a four week exposure time (1, 8, 24, 48, 120, 168, 264, 336, 504 and 672 hrs). The effect of water flow rate on the persistence of fibres was investigated by conducting the experiment with two flow velocities; 'high' (â¼2.75 L/s) or 'low' (â¼0.7 L/s). Significant differences between textile type were seen at 504 hrs under low flow conditions and 8, 24, 168 and 264 hrs under high flow conditions. When comparing flow velocities, a significant difference was seen at 1 hr exposure for the fleece textile only, indicating that the two flow rates used in this study do not significantly affect fibre persistence. Initial loss rates were highest for the first hour of submergence for the carpet, fleece and sports vest. Fibre persistence rates were highest on the carpet, followed by fleece and then sports vest. Persistence rates remained mostly constant after 24 hrs for all textiles but with redistribution of fibres between textiles being seen after this exposure time. The use of artificial flumes in this study provided a balance between realistic experimentation and a controlled study; key experimental variables could be continously and safely monitored. This study provides the first fibre persistence data in river type environments and proposes a new method for testing persistence in aquatic environments. This approach is not limited to fibres evidence and could be employed for other evidence such as glass, pollen, fingerprints and DNA.
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Global urbanization trends have led to the widespread increasing occurrence of contaminants of emerging concern (CECs) such as pharmaceuticals, personal care products, pesticides, and micro- and nano-plastics in aquatic systems. Even at low concentrations, these contaminants pose a threat to aquatic ecosystems. To better understand the effects of CECs on aquatic ecosystems, it is important to measure concentrations of these contaminants present in these systems. Currently, there is an imbalance in CEC monitoring, with more attention to some categories of CECs, and a lack of data about environmental concentrations of other types of CECs. Citizen science is a potential tool for improving CEC monitoring and to establish their environmental concentrations. However, incorporating citizen participation in the monitoring of CECs poses some challenges and questions. In this literature review, we explore the landscape of citizen science and community science projects which monitor different groups of CECs in freshwater and marine ecosystems. We also identify the benefits and drawbacks of using citizen science to monitor CECs to provide recommendations for sampling and analytical methods. Our results highlight an existing disparity in frequency of monitoring different groups of CECs with implementing citizen science. Specifically, volunteer participation in microplastic monitoring programs is higher than volunteer participation in pharmaceutical, pesticide, and personal care product programs. These differences, however, do not necessarily imply that fewer sampling and analytical methods are available. Finally, our proposed roadmap provides guidance on which methods can be used to improve monitoring of all groups of CECs through citizen science.
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Ciência do Cidadão , Praguicidas , Poluentes Químicos da Água , Humanos , Poluentes Químicos da Água/análise , Ecossistema , Plásticos , Monitoramento Ambiental/métodos , Praguicidas/análiseRESUMO
A variety of organisms can colonize microplastic surfaces through biofouling processes. Heterotrophic bacteria tend to be the focus of plastisphere research; however, the presence of epiplastic microalgae within the biofilm has been repeatedly documented. Despite the relevance of biofouling in determining the fate and effects of microplastics in aquatic systems, data about this process are still scarce, especially for freshwater ecosystems. Here, our goal was to evaluate the biomass development and species composition of biofilms on different plastic polymers and to investigate whether plastic substrates exert a strong enough selection to drive species sorting, overcoming other niche-defining factors. We added microplastic pellets of high-density polyethylene (HDPE), polyethylene terephthalate (PET), and a mix of the two polymers in 15 lentic mesocosms in five different locations of the Iberian Peninsula, and after one month, we evaluated species composition and biomass of microalgae developed on plastic surfaces. Our results, based on 45 samples, showed that colonization of plastic surfaces occurred in a range of lentic ecosystems covering a wide geographical gradient and different environmental conditions (e.g., nutrient concentration, conductivity, macrophyte coverage). We highlighted that total biomass differed based on the polymer considered, with higher biomass developed on PET substrate compared to HDPE. Microplastics supported the growth of a rich and diversified community of microalgae (242 species), with some cosmopolite species. However, we did not observe species-specificity in the colonization of the different plastic polymers. Local species pool and nutrient concentration rather than polymeric composition seemed to be the determinant factor defying the community diversity. Regardless of specific environmental conditions, we showed that many species could coexist on the surface of relatively small plastic items, highlighting how microplastics may have considerable carrying capacity, with possible consequences on the wider ecological context.
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Microalgas , Poluentes Químicos da Água , Ecossistema , Monitoramento Ambiental , Microplásticos , Plásticos , Polímeros/química , Poluentes Químicos da Água/análiseRESUMO
Raman spectroscopy is gaining ground in the analysis of microplastics, especially due to its high spatial resolution that allows the investigation of small plastic particles, whose numeric abundance is argued to be particularly relevant in aquatic systems. Here, we aimed at outlining the status of Raman analysis of microplastics from aquatic systems, highlighting the advantages and the drawbacks of this technique and critically presenting tools and ways to effectively employ this instrument and to improve the spectra obtained and their interpretation. In particular, we summarized procedural information for the use of Raman spectroscopy, and we discussed issues linked to fluorescence interference and the analysis of weathered polymers, which may complicate the interpretation of Raman signatures. In this context, a deep understanding of the different plastic polymers and their Raman peaks and chemical fingerprints is fundamental to avoid misidentification. Therefore, we provided a catalog with detailed information about peaks of most common plastic polymers, and this represents, to the best of our knowledge, the first comprehensive resource that systematically synthesized plastic Raman peaks. Additionally, we focused on plastic additives, which are contained in the majority of plastics. These compounds are often intense in Raman scattering and may partly or completely overlie the actual material types, resulting in the identification of additives alone or misidentification issue. For these reasons, we also presented a new R package "RamanMP" that includes a database of 356 spectra (325 of which are additives). This will help to foster the use of this technique, which is becoming especially relevant in microplastic analysis.
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Microplásticos , Poluentes Químicos da Água , Monitoramento Ambiental , Plásticos , Análise Espectral Raman , Poluentes Químicos da Água/análiseRESUMO
Climate change and other anthropogenic stressors have led to long-term changes in the thermal structure, including surface temperatures, deepwater temperatures, and vertical thermal gradients, in many lakes around the world. Though many studies highlight warming of surface water temperatures in lakes worldwide, less is known about long-term trends in full vertical thermal structure and deepwater temperatures, which have been changing less consistently in both direction and magnitude. Here, we present a globally-expansive data set of summertime in-situ vertical temperature profiles from 153 lakes, with one time series beginning as early as 1894. We also compiled lake geographic, morphometric, and water quality variables that can influence vertical thermal structure through a variety of potential mechanisms in these lakes. These long-term time series of vertical temperature profiles and corresponding lake characteristics serve as valuable data to help understand changes and drivers of lake thermal structure in a time of rapid global and ecological change.
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This study presents the findings from several field campaigns carried out in Lake Idro (Northern Italy), a deep (124 m) meromictic-subalpine lake, whose water column is subdivided in a mixolimnion (~0-40 m) and a monimolimnion (~40-124 m). Hydrochemical data highlight two main peculiarities characterizing the Lake Idro meromixis: a) presence of a high manganese/iron ratio (up to 20 mol/mol), b) absence of a clear chemocline between the two main layers. The high manganese content contributed to the formation of a stable manganese dominated deep turbid stratum (40-65 m), enveloping the redoxcline (~45-55 m) in the upper monimolimnion. The presence of this turbid stratum in Lake Idro is described for the first time in this study. The paper examines the distribution of dissolved and particulate forms of transition metals (Mn and Fe), alkaline earth metals (Ca and Mg), and other macro-constituents or nutrients (S, P, NO3-N, NH4-N), discussing their behavior over the redoxcline, where the main transition processes occur. Field measurements and theoretical considerations suggest that the deep turbid stratum is formed by a complex mixture of manganese and iron compounds with a prevalence of Mn(II)/Mn(III) in different forms including dissolved, colloidal, and fine particles, that give to the turbid stratum a white-pink opalescent coloration. The bacteria populations show a clear stratification with the upper aerobic layer dominated by the heterotrophic Flavobacterium sp., the turbid stratum hosting a specific microbiological pool, dominated by Caldimonas sp., and the deeper anaerobic layer dominated by the sulfur-oxidizing and denitrifier Sulfuricurvum sp. The occurrence in August 2010 of an anomalous lake surface coloration lasting about four weeks and developing from milky white-green to red-brown suggests that the upper zone of the turbid stratum could be eroded during intense weather-hydrological conditions with the final red-brown coloration resulting from the oxidation of Mn(II)/Mn(III) to Mn(IV) compounds.
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Lagos , Manganês , Bactérias , Oxirredução , EnxofreRESUMO
The concentration of dissolved oxygen in aquatic systems helps to regulate biodiversity1,2, nutrient biogeochemistry3, greenhouse gas emissions4, and the quality of drinking water5. The long-term declines in dissolved oxygen concentrations in coastal and ocean waters have been linked to climate warming and human activity6,7, but little is known about the changes in dissolved oxygen concentrations in lakes. Although the solubility of dissolved oxygen decreases with increasing water temperatures, long-term lake trajectories are difficult to predict. Oxygen losses in warming lakes may be amplified by enhanced decomposition and stronger thermal stratification8,9 or oxygen may increase as a result of enhanced primary production10. Here we analyse a combined total of 45,148 dissolved oxygen and temperature profiles and calculate trends for 393 temperate lakes that span 1941 to 2017. We find that a decline in dissolved oxygen is widespread in surface and deep-water habitats. The decline in surface waters is primarily associated with reduced solubility under warmer water temperatures, although dissolved oxygen in surface waters increased in a subset of highly productive warming lakes, probably owing to increasing production of phytoplankton. By contrast, the decline in deep waters is associated with stronger thermal stratification and loss of water clarity, but not with changes in gas solubility. Our results suggest that climate change and declining water clarity have altered the physical and chemical environment of lakes. Declines in dissolved oxygen in freshwater are 2.75 to 9.3 times greater than observed in the world's oceans6,7 and could threaten essential lake ecosystem services2,3,5,11.
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Lagos/química , Oxigênio/análise , Oxigênio/metabolismo , Temperatura , Animais , Mudança Climática , Ecossistema , Oceanos e Mares , Oxigênio/química , Fitoplâncton/metabolismo , Solubilidade , Fatores de TempoRESUMO
With the widespread occurrence of microplastics in aquatic ecosystems having been firmly established, the focus of research has shifted towards the assessments of their influence on ecosystem functions and food webs. This includes interactions between microplastics and microalgae, as fundamental components at the base of aquatic food webs and pivotal organisms in a wide range of ecosystem functions. In this review, we present the current state of knowledge on microalgae-microplastic interactions and summarize the potential effect on their respective fate. Microplastics can and do interact with microalgae and the available literature has suggested that the epiplastic community of microalgae differs consistently from the surrounding aquatic communities; however, it is still not clear whether this different colonization is linked to the composition of the surface or more to the availability of a "hard" substrate on which organisms can attach and grow. Further studies are needed to understand to what extent the properties of different plastic materials and different environmental factors may affect the growth of microalgae on plastic debris. Biofouling may alter microplastic properties, especially increasing their density, consequently affecting the vertical fluxes of plastics. Moreover, microplastics may have toxic effects on microalgae, which could be physical or related to chemical interactions with plasticizers or other chemicals associated with plastics, with consequences for algal growth, photosynthetic activity, and morphology. Microplastics seems to have the potential to affect not only the quality (e.g., fatty acids and lipids composition, food dilution effect) but also the quantity of algal production, both positively and negatively. This may have consequences for energy fluxes, which may propagate throughout the whole food web and alter aquatic productivity. Even though experimental results have indicated reciprocal impacts between plastics and microalgae, it is currently difficult to predict how these impacts may manifest themselves at the ecosystem level. Therefore, further studies are needed to address this important topic.
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Microalgas , Poluentes Químicos da Água , Organismos Aquáticos , Ecossistema , Monitoramento Ambiental , Microplásticos , Plásticos , Poluentes Químicos da Água/análiseRESUMO
Understanding the factors that control As concentrations in groundwater is vital for supplying safe groundwater in regions with As-polluted aquifers. Despite much research, mainly addressing Holocene aquifers hosting young (<100 yrs) groundwater, the source, transport, and fate of As in Pleistocene aquifers with fossil (>12,000 yrs) groundwaters are not yet fully understood and so are assessed here through an evaluation of the redox properties of the system in a type locality, the Po Plain (Italy). Analyses of redox-sensitive species and major ions on 22 groundwater samples from the Pleistocene arsenic-affected aquifer in the Po Plain shows that groundwater concentrations of As are controlled by the simultaneous operation of several terminal electron accepters. Organic matter, present as peat, is abundant in the aquifer, allowing groundwater to reach a quasi-steady-state of highly reducing conditions close to thermodynamic equilibrium. In this system, simultaneous reduction of Fe-oxide and sulfate results in low concentrations of As (median 7 µg/L) whereas As reaches higher concentrations (median of 82 µg/L) during simultaneous methanogenesis and Fe-reduction. The position of well-screens is an additional controlling factor on groundwater As: short screens that overlap confining aquitards generate higher As concentrations than long screens placed away from them. A conceptual model for groundwater As, applicable worldwide in other Pleistocene aquifers with reducible Fe-oxides and abundant organic matter is proposed: As may have two concentration peaks, the first after prolonged Fe-oxide reduction and until sulfate reduction takes place, the second during simultaneous Fe-reduction and methanogenesis.
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In this study, sources of groundwater pollution in a landfill site were identified, using artificial sweeteners as chemical tracers, multivariate statistical analysis and a quantitative analysis of the groundwater flow system through particle tracking and transport modeling. The study area, located in northern Italy, hosts an older unlined landfill and a newer lined municipal solid waste landfill placed downstream of the former. Groundwater, surface water, treated wastewater, and leachate samples were collected in March 2017 for analysis of the artificial sweeteners saccharin, cyclamate, acesulfame and sucralose together with major cations and anions, inorganic nitrogen compounds, total phosphorus, COD and some further parameters. The interpretation of the results suggests that two main leachate leaks/spills are affecting the study area. The first one concerns leachate probably spilling out of the leachate collection system serving the younger lined landfill, the other one involves leachate from the older unlined landfill that also seems to affect an area downstream of the lined landfill. Direct leachate leaks from the lined landfill seem unlikely, although they cannot be definitively excluded. This work underlines the importance of a multi-methods approach, which integrates here chemical tracers, multivariate analysis and transport modeling, for assessing groundwater pollution sources generated from complex landfill sites, where multiple and different sources may exist. In particular, this work highlights how artificial sweeteners can be used for tracing leachate plumes from landfills. The methodology applied in this study can have a broad applicability also in other polluted landfill sites worldwide.
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Água Subterrânea , Eliminação de Resíduos , Poluentes Químicos da Água , Monitoramento Ambiental , Itália , Análise Multivariada , Edulcorantes , Instalações de Eliminação de ResíduosRESUMO
The impact of microplastics (MPs) on reef-building corals are still largely unknown. The scientific literature provides evidence from lab feeding trials that coral may ingest MPs. Several adverse effects, i.e., necrosis and bleaching, have also been highlighted. However, field studies are limited. Here, we investigated for the first time the possible correlation between MP seawater contamination and the presence of phthalic acid esters (PAEs), a class of MP-associated contaminants, in scleractinian corals. The survey was carried out in a remote coral reef atoll in the Indian Ocean located in the Maldivian archipelago, considered as a case study. MPs and PAEs were monitored in subsurface neustonic tow samples and scleractinian corals across twelve sampling sites. The results showed widespread MP contamination and the presence of appreciable levels of PAEs in the scleractinian corals sampled inside the atoll rim near an inhabited island, which correlated with the highest MP concentration.
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Antozoários/química , Ácidos Ftálicos/análise , Plásticos/análise , Poluentes Químicos da Água/análise , Animais , Antozoários/efeitos dos fármacos , Recifes de Corais , Ecotoxicologia/métodos , Ésteres/análise , Oceano Índico , Ilhas do Oceano Índico , Ácidos Ftálicos/toxicidade , Plásticos/toxicidade , Água do Mar , Poluentes Químicos da Água/toxicidade , Poluição Química da Água/análiseRESUMO
For several hundred years, farming in the Po Plain of Italy (46,000â¯km2, 20 million inhabitants) has been supported by intensive surface irrigation with lake and river water. Despite the longevity of irrigation, its effects on the quality and quantity of groundwater is poorly known and so is investigated here through seasonal measurements of hydraulic heads and water quality in groundwaters, rivers, lake, springs and rainwaters. In the north of the study region, an unconfined coarse-grained alluvial aquifer, infiltration of surface irrigation water, sourced from the Oglio River and low in NO3, contributes much to aquifer recharge (up to 88%, as evidenced by a δ2H-Cl/Br mixing model) and has positive effects on groundwater quality by diluting high concentrations of NO3 (decrease by 17% between June and September). This recharge also helps to maintain numerous local springs that form important local micro-environments. Any increase in water-use efficiency in irrigation will reduce this recharge, imperil the spring environments, and lessen the dilution of NO3 leading to increasing NO3 concentrations in groundwater. These findings can be extended by analogy to the entire Po Plain region and other surface-water-irrigated systems worldwide where inefficient irrigation methods are used and similar hydrogeological features occur.
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A first synoptic and trans-domain overview of plankton dynamics was conducted across the aquatic sites belonging to the Italian Long-Term Ecological Research Network (LTER-Italy). Based on published studies, checked and complemented with unpublished information, we investigated phytoplankton and zooplankton annual dynamics and long-term changes across domains: from the large subalpine lakes to mountain lakes and artificial lakes, from lagoons to marine coastal ecosystems. This study permitted identifying common and unique environmental drivers and ecological functional processes controlling seasonal and long-term temporal course. The most relevant patterns of plankton seasonal succession were revealed, showing that the driving factors were nutrient availability, stratification regime, and freshwater inflow. Phytoplankton and mesozooplankton displayed a wide interannual variability at most sites. Unidirectional or linear long-term trends were rarely detected but all sites were impacted across the years by at least one, but in many case several major stressor(s): nutrient inputs, meteo-climatic variability at the local and regional scale, and direct human activities at specific sites. Different climatic and anthropic forcings frequently co-occurred, whereby the responses of plankton communities were the result of this environmental complexity. Overall, the LTER investigations are providing an unparalleled framework of knowledge to evaluate changes in the aquatic pelagic systems and management options.
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Ecossistema , Monitoramento Ambiental , Plâncton/fisiologia , Animais , Itália , Fitoplâncton , Dinâmica Populacional , ZooplânctonRESUMO
BTEX compounds (Benzene, Toluene, Ethylbenzene and Xylenes) are toxic hydrocarbons that can be found in groundwater due to accidental spills. Bioelectrochemical systems (BES) are an innovative technology to stimulate the anaerobic degradation of hydrocarbons. In this work, single chamber BESs were used to assess the degradation of a BTEX mixture at different applied voltages (0.8V, 1.0V, 1.2V) between the electrodes. Hydrocarbon degradation was linked to current production and to sulfate reduction, at all the tested potentials. The highest current densities (about 200mA/m2 with a maximum peak at 480mA/m2) were observed when 0.8V were applied. The application of an external voltage increased the removal of toluene, m-xylene and p-xylene. The highest removal rate constants at 0.8V were: 0.4±0.1days-1, 0.34±0.09days-1 and 0.16±0.02days-1, respectively. At the end of the experiment, the microbial communities were characterized by high throughput sequencing of the 16S rRNA gene. Microorganisms belonging to the families Desulfobulbaceae, Desulfuromonadaceae and Geobacteraceae were enriched on the anodes suggesting that both direct electron transfer and sulfur cycling occurred. The cathodic communities were dominated by the family Desulfomicrobiaceae that may be involved in hydrogen production.
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Bactérias/metabolismo , Derivados de Benzeno , Benzeno , Tolueno , Poluentes Químicos da Água , Xilenos , Bactérias/genética , Benzeno/química , Benzeno/metabolismo , Derivados de Benzeno/química , Derivados de Benzeno/metabolismo , Biodegradação Ambiental , Técnicas Eletroquímicas , Eletrodos/microbiologia , Oxirredução , RNA Ribossômico 16S/genética , Tolueno/química , Tolueno/metabolismo , Poluentes Químicos da Água/química , Poluentes Químicos da Água/metabolismo , Xilenos/química , Xilenos/metabolismoRESUMO
In this work, a fixed-dose combination of gabapentin and flurbiprofen formulated as multilayer tablets has been designed, developed and studied in vitro and in vivo. The aim was to construct a single dosage form of the two drugs, able to perform a therapeutic program involving three release kinetics and two delivery sites, i.e., immediate release of gabapentin, intra-gastric prolonged release of gabapentin and intestinal (delayed) release of flurbiprofen. An oblong three-layer tablet was manufactured having as top layer a floating hydrophilic polymeric matrix for gastric release of gabapentin, as middle layer a disintegrating formulation for immediate release of a gabapentin loading dose and as bottom layer, an uncoated hydrophilic polymeric matrix, swellable but insoluble in gastric fluids, for delayed and prolonged release of flurbiprofen in intestinal environment. The formulations were studied in vitro and in vivo in healthy volunteers. The in vitro release rate assessment confirmed the programmed delivery design. A significant higher bioavailability of gabapentin administered 30min after meal, compared to fasting conditions or to dose administration 10min before meal, argued in favor of the gastro-retention of gabapentin prolonged release layer. The two drugs were delivered at different anatomical sites, since the food presence prolonged the gastric absorption of gabapentin from the floating layer and delayed the flurbiprofen absorption. The attainment of a successful delayed release of flurbiprofen was realized by a matrix based on a polymers' combination. The combined use of three hydrophilic polymers with different pH sensitivity provided the dosage form layer containing flurbiprofen with gastro-resistant characteristics without the use of film coating.
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Aminas/administração & dosagem , Aminas/farmacocinética , Ácidos Cicloexanocarboxílicos/administração & dosagem , Ácidos Cicloexanocarboxílicos/farmacocinética , Flurbiprofeno/administração & dosagem , Flurbiprofeno/farmacocinética , Ácido gama-Aminobutírico/administração & dosagem , Ácido gama-Aminobutírico/farmacocinética , Administração Oral , Adolescente , Adulto , Analgésicos/administração & dosagem , Analgésicos/farmacocinética , Anti-Inflamatórios não Esteroides/administração & dosagem , Anti-Inflamatórios não Esteroides/farmacocinética , Estudos Cross-Over , Preparações de Ação Retardada/administração & dosagem , Preparações de Ação Retardada/farmacocinética , Combinação de Medicamentos , Liberação Controlada de Fármacos , Feminino , Interações Alimento-Droga , Agonistas GABAérgicos/administração & dosagem , Agonistas GABAérgicos/farmacocinética , Gabapentina , Mucosa Gástrica/metabolismo , Humanos , Mucosa Intestinal/metabolismo , Masculino , Pessoa de Meia-Idade , Polímeros/administração & dosagem , Polímeros/farmacocinética , Comprimidos , Adulto JovemRESUMO
The fat content and the fatty acid composition of the roe of European catfish (Silurus glanis) wild specimens captured in the Lambro river basin (Como, Northern Italy) were investigated in order to assess their nutritional value. Total extracted lipid values on roe weight were 5.8-6.3%. Phospholipids (40.2-43.6%) and triacylglycerols (31.8-34.7%) were the most represented lipid classes. Relevant amount of cholesteryl esters (14.3-15.6%) were also detected. The main fatty acids were palmitic, oleic and cis-4,7,10,13,16,19-docosahexaenoic acid (DHA). Total saturated fatty acids were 28.1-30.9%, monounsaturated fatty acids were 28.2-30.6%, and polyunsaturated fatty acids (PUFAs) were 26.7-29.1%. The good found content of phospholipids and ω3-polyunsaturated fatty acids encourage further investigation for the exploitation of Silurus glanis roe in food products and/or supplements.
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Peixes-Gato , Embrião não Mamífero/química , Animais , Peixes-Gato/embriologia , Peixes-Gato/metabolismo , Ácidos Graxos , Fosfolipídeos , TriglicerídeosRESUMO
The oxidized version of Avidin, known as AvidinOX, was previously shown to link to tissue proteins upon injection or nebulization, thus becoming a stable receptor for biotinylated therapeutics. AvidinOX is currently under clinical investigation to target radioactive biotin to inoperable tumor lesions (ClinicalTrials.gov NCT02053324). Presently, we show that the anti-ErbB2 monoclonal antibodies Trastuzumab and Pertuzumab can be chemically biotinylated while maintaining their biochemical and biological properties. By using several and diverse experimental conditions, we show that when AvidinOX is conjugated to tumor cells, low antibody concentrations of biotinylated Trastuzumab (bTrast) or Pertuzumab (bPert) prevent internalization of ErbB2, induce endoplasmic reticulum stress, cell cycle arrest and apoptosis leading to inhibition of proliferation and ErbB2 signaling. Moreover, we found that the treatment is able to induce down-modulation of ErbB2 thus bypassing the known resistance of this receptor to degradation. Interestingly, we show that AvidinOX anchorage is a way to counteract agonistic activities of Trastuzumab and Pertuzumab. Present data are in agreement with previous observations from our group indicating that the engagement of the Epidermal Growth Factor Receptor (EGFR) by AvidinOX-bound biotinylated Cetuximab or Panitumumab, leads to potent tumor inhibition both in vitro and in animal models. All results taken together encourage further investigation of AvidinOX-based treatments with biotinylated antibodies directed to the members of the EGFR family.
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Protocolos de Quimioterapia Combinada Antineoplásica/farmacologia , Apoptose/efeitos dos fármacos , Avidina/metabolismo , Neoplasias da Mama/tratamento farmacológico , Receptor ErbB-2/antagonistas & inibidores , Anticorpos Monoclonais Humanizados/administração & dosagem , Avidina/química , Biomarcadores Tumorais/metabolismo , Biotinilação , Neoplasias da Mama/metabolismo , Neoplasias da Mama/patologia , Proliferação de Células/efeitos dos fármacos , Feminino , Humanos , Receptor ErbB-2/imunologia , Transdução de Sinais , Trastuzumab/administração & dosagem , Células Tumorais CultivadasRESUMO
The availability of more than thirty years of historical satellite data is a valuable source which could be used as an alternative to the sparse in-situ data. We developed a new homogenised time series of daily day time Lake Surface Water Temperature (LSWT) over the last thirty years (1986-2015) at a spatial resolution of 1km from thirteen polar orbiting satellites. The new homogenisation procedure implemented in this study corrects for the different acquisition times of the satellites standardizing the derived LSWT to 12:00 UTC. In this study, we developed new time series of LSWT for five large lakes in Italy and evaluated the product with in-situ data from the respective lakes. Furthermore, we estimated the long-term annual and summer trends, the temporal coherence of mean LSWT between the lakes, and studied the intra-annual variations and long-term trends from the newly developed LSWT time series. We found a regional warming trend at a rate of 0.017°Cyr-1 annually and 0.032°Cyr-1 during summer. Mean annual and summer LSWT temporal patterns in these lakes were found to be highly coherent. Amidst the reported rapid warming of lakes globally, it is important to understand the long-term variations of surface temperature at a regional scale. This study contributes a new method to derive long-term accurate LSWT for lakes with sparse in-situ data thereby facilitating understanding of regional level changes in lake's surface temperature.