Forecasting German crash numbers: The effect of meteorological variables.

At the end of each year, the German Federal Highway Research Institute (BASt) publishes the road safety balance of the closing year. They describe the development of accident and casualty numbers disaggregated by road user types, age groups, type of road and the consequences of the accidents. However, at the time of publishing, these series are only available for the first eight or nine months of the year. To make the balance for the whole year, the last three or four months are forecasted. The objective of this study was to improve the accuracy of these forecasts through structural time-series models that include effects of meteorological conditions. The results show that, compared to the earlier heuristic approach, root mean squared errors are reduced by up to 55% and only two out of the 27 different data series yield a modest rise of prediction errors. With the exception of four data series, prediction accuracies also clearly improve incorporating meteorological data in the analysis. We conclude that our approach provides a valid alternative to provide input to policy makers in Germany.

Formation of a physiological reverse shoulder joint.

Congenital shoulder deformities are rarely seen in orthopaedic practice. Proximal humeral defects and glenoid hypoplasia have been reported separately in the literature. We present a case involving a 31-year-old woman having a cosmetic problem with her upper arm who was diagnosed with reverse shoulder joint deformity. This article presents the clinical, radiological and biomechanical findings of a physiological reverse shoulder joint. This is the first such reported case.

An extracellular loop of the human non-gastric H,K-ATPase alpha-subunit is involved in apical plasma membrane polarization.

The human non-gastric H,K-ATPase, ATP1AL1, belongs to the gene family of P-type ATPases. Consistent with their physiological roles in ion transport, members of this group, including the Na,KATPase and the gastric and non-gastric H,K-ATPases, are differentially polarized to either the basolateral or apical plasma membrane in epithelial cells. However, their polarized distribution is highly complex and depends on specific sorting signals or motifs which are recognized by the subcellular targeting machinery. For the gastric H,K-ATPase it has been suggested that the 4(th) transmembrane spanning domain (TM4) and its flanking regions induce conformational sorting motifs which direct the ion pump exclusively to the epithelial apical membrane. Here, we show in transfected Madin-Darby canine kidney (MDCK) cells that the related non-gastric H,KATPase, ATP1AL1, does contain similar sorting motifs in close proximity to TM4. A short extracellular loop between TM3 and TM4 is critical for this pump's apical delivery. A single point mutation in the corresponding region redirects ATP1AL1 to the basolateral membrane. In conclusion, our work provides further evidence that the cellular distribution of P-type ATPases is determined by conformational sorting motifs.