RESUMO
A single subcutaneous injection of a long-acting immobilized insulin preparation activated biosynthetic events in the liver and skin of the burnt animals and slowed down tissue degradation as evidenced by creatine excretion. This insulin preparation can be successfully used for the treatment of burnt patients in order to stimulate metabolism and maintain normoglycemia.
Assuntos
Queimaduras/tratamento farmacológico , Insulina/uso terapêutico , Animais , Queimaduras/metabolismo , Colinesterases/sangue , Creatina/urina , Preparações de Ação Retardada , Avaliação Pré-Clínica de Medicamentos , Fígado/metabolismo , Masculino , Ratos , Pele/metabolismo , SuspensõesRESUMO
As compared with the known long-acting insulin preparations, immobilized insulin produces an earlier (starting from the first hour) and more prolonged hypoglycemic action in experimental animals (in the intact and in those with induced diabetes) after a single subcutaneous injection.
Assuntos
Diabetes Mellitus Experimental/tratamento farmacológico , Insulina/uso terapêutico , Animais , Glicemia/análise , Preparações de Ação Retardada , Eritrócitos , Humanos , Insulina/administração & dosagem , Coelhos , Ratos , Fatores de TempoAssuntos
Arginase , Plaquetas , Eritrócitos , Glucose Oxidase , Peroxidases , Adsorção , Animais , Enzimas Imobilizadas , Temperatura Alta , Humanos , Técnicas In Vitro , CoelhosRESUMO
Tests set up on rats and rabbits showed the ability of phospho-enolpyruvate and pyruvate-kinase to lower the aggregation of thrombocytes and prevent formation of experimental microthrombosis. The joint introduction of these compounds in most experiments resulted in potentiation of the antithrombotic effect.
Assuntos
Fibrinolíticos , Fosfoenolpiruvato/farmacologia , Agregação Plaquetária/efeitos dos fármacos , Piruvato Quinase/farmacologia , Trombose/prevenção & controle , Animais , Sinergismo Farmacológico , Fibrinolíticos/uso terapêutico , Fosfoenolpiruvato/uso terapêutico , Piruvato Quinase/uso terapêutico , Coelhos , Ratos , Trombose/sangueRESUMO
Activities of lactate dehydrogenase, glucose-6-phosphate dehydrogenase, transketolase and creatine phosphokinase were studied in tissue of aneurysm and in extrainfarctional part of rat heart muscle after experimental infarction. Activity of total LDH, content of its anode isoenzymes and creatine phosphokinase activity were decreased. Enzymes of pentose phosphate pathway were activated and amount of cathode LDH isoenzymes was increased. The most distinct and apparently irreversible alterations in the enzymatic activity were observed in the aneurysm tissues.
Assuntos
Creatina Quinase/metabolismo , Glucosefosfato Desidrogenase/metabolismo , Aneurisma Cardíaco/enzimologia , L-Lactato Desidrogenase/metabolismo , Infarto do Miocárdio/enzimologia , Miocárdio/enzimologia , Transcetolase/metabolismo , Animais , Aneurisma Cardíaco/etiologia , Infarto do Miocárdio/complicações , RatosRESUMO
Distribution of peroxidase was studied in structures and soluble fractions of cytoplasma and nuclei of washed cells of Erhlich carcinoma, obtained from mice, previously administered intraperitoneally with the enzyme. Peroxidase was shown to penetrate in all the cell fractions studied; the most specific activity of the extraneous enzyme was observed in mitochondria.