TRPC6 Knockout Alleviates Renal Fibrosis through PI3K/AKT/GSK3B Pathway.

OBJECTIVE: Renal fibrosis is the ultimate pathway of various forms of acute and chronic kidney damage. Notably, the knockout of transient receptor potential channel 6 (TRPC6) has shown promise in alleviating renal fibrosis. However, the regulatory impact of TRPC6 on renal fibrosis remains unclear. METHODS: In vivo, TRPC6 knockout (TRPC6-/-) mice and age-matched 129 SvEv (WT) mice underwent unilateral renal ischemia-reperfusion (uIR) injury surgery on the left renal pedicle or sham operation. Kidneys and serum were collected on days 7, 14, 21, and 28 after euthanasia. In vitro, primary tubular epithelial cells (PTECs) were isolated from TRPC6-/- and WT mice, followed by treatment with transforming growth factor ß1 (TGFß1) for 72 h. The anti-fibrotic effect of TRPC6-/- and the underlying mechanisms were assessed through hematoxylin-eosin staining, Masson staining, immunostaining, qRT-PCR, and Western blotting. RESULTS: Increased TRPC6 expression was observed in uIR mice and PTECs treated with TGFß1. TRPC6-/- alleviated renal fibrosis by reducing the expression of fibrotic markers (Col-1, α-SMA, and vimentin), as well as decreasing the apoptosis and inflammation of PTECs during fibrotic progression both in vivo and in vitro. Additionally, we found that the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (AKT)/glycogen synthase kinase 3 beta (GSK3ß) signaling pathway, a pivotal player in renal fibrosis, was down-regulated following TRPC6 deletion. CONCLUSION: These results suggest that the ablation of TRPC6 may mitigate renal fibrosis by inhibiting the apoptosis and inflammation of PTECs through down-regulation of the PI3K/AKT/GSK3ß pathway. Targeting TRPC6 could be a novel therapeutic strategy for preventing chronic kidney disease.

Study of CuSb bimetallic flow-through gas diffusion electrodes for efficient electrochemical CO2 reduction to CO.

Electrochemical CO2 reduction (eCO2R) to industrially important feedstock has received great attention, but it faces different challenges. Among them, the poor CO2 mass transport due to low intrinsic CO2 solubility significantly limits the rate of reduction reactions, leading to lower catalytic performance; thereby, commercially relevant current densities can't be achieved. Moreover, the poor activity and selectivity of high-cost monometallic catalysts, including Cu, Zn, Ag, and Au, undermine the efficiency of eCO2R. Flow-through gas diffusion electrodes (FTGDE), a newly developed class of GDEs, can potentially solve the issue of poor CO2 mass transport because they directly feed the CO2 to the catalyst layer. In addition, abundant surface area, porous structure, and improved triple-phase interface make them an excellent candidate for extremely high rate eCO2R. Antimony, a low-cost and abundant metalloid, can be effectively tuned with Cu to produce useful products such as CO, formate, and C2H4 through eCO2R. Herein, a series of porous binary CuSb FTGDEs with different Sb compositions are fabricated for the electrocatalytic reduction of CO2 to CO. The results show that the catalytic performance of CuSb FTGDEs improved with increasing Sb content up to a certain threshold, beyond which it started to decrease. The CuSb FTGDE with 5.4 g of antimony demonstrated higher current density (206.4 mA/cm2) and faradaic efficiency (72.82 %) at relatively lower overpotentials. Compared to gas diffusion configuration, the poor catalytic activity and selectivity achieved by CuSb FTGDE in non-gas diffusion configuration signifies the importance of improved local CO2 concentration and improved triple-phase interface formation in GDE configuration. The several hours stable operation of CuSb FTGDEs during eCO2R demonstrates its potential for efficient electrocatalytic conversion applications.

Dosimetric impact of CT metal artifact reduction for spinal implants in stereotactic body radiotherapy planning.

Background: Metal artifacts due to spinal implants may affect the accuracy of dose calculation for radiotherapy. However, the dosimetric impact of metal artifact reduction (MAR) for spinal implants in stereotactic body radiotherapy (SBRT) plans has not been well studied. The objective of this study was to evaluate the dosimetric impact of MAR in spinal SBRT planning with three clinically common dose calculation algorithms. Methods: Gammex phantom and 10 patients' computed tomography (CT) images were studied to investigate the effects of titanium implants. A commercial orthopedic MAR algorithm was employed to reduce artifacts. Dose calculations for SBRT were conducted on both artifact-corrected and uncorrected images using three commercial algorithms [analytical anisotropic algorithm (AAA), Acuros XB (AXB), and Monte Carlo (MC)]. Dose discrepancies between artifact-corrected and uncorrected cases were appraised using a dose-volume histogram (DVH) and 3-dimensional (3D) gamma analysis with different distance to agreement (DTA) and dose difference criteria. The gamma agreement index (GAI) was denoted as G(∆D, DTA). Statistical analysis of t-test was utilized to evaluate the dose differences of different algorithms. Results: The phantom study demonstrated that titanium metal artifacts can be effectively reduced. The patient cases study showed that dose differences between the artifact-corrected and uncorrected datasets were small evaluated by gamma index and DVH. Gamma analysis found that even the strict criterion local G(1,1) had average values ≥93.9% for the three algorithms. For all DVH metrics, average differences did not exceed 0.7% in planning target volume (PTV) and 2.1% in planning risk volume of spinal cord (PRV-SC). Statistical analysis showed that the observed dose differences of MC method were significantly larger than those of AAA (P<0.01 for D98% of PTV and P<0.001 for D0.1cc of spinal cord) and AXB methods (P<0.001 for D98% and P<0.0001 for D0.1cc). Conclusions: Dosimetric impact of artifacts caused by titanium implants is not significant in spinal SBRT planning, which indicates that dose calculation algorithms might not be very sensitive to CT number variation caused by titanium inserts.

Chemical Inhibition of RPA by HAMNO Alters Cell Cycle Dynamics by Impeding DNA Replication and G2-to-M Transition but Has Little Effect on the Radiation-Induced DNA Damage Response.

Replication protein A (RPA) is the major single-stranded DNA (ssDNA) binding protein that is essential for DNA replication and processing of DNA double-strand breaks (DSBs) by homology-directed repair pathways. Recently, small molecule inhibitors have been developed targeting the RPA70 subunit and preventing RPA interactions with ssDNA and various DNA repair proteins. The rationale of this development is the potential utility of such compounds as cancer therapeutics, owing to their ability to inhibit DNA replication that sustains tumor growth. Among these compounds, (1Z)-1-[(2-hydroxyanilino) methylidene] naphthalen-2-one (HAMNO) has been more extensively studied and its efficacy against tumor growth was shown to arise from the associated DNA replication stress. Here, we study the effects of HAMNO on cells exposed to ionizing radiation (IR), focusing on the effects on the DNA damage response and the processing of DSBs and explore its potential as a radiosensitizer. We show that HAMNO by itself slows down the progression of cells through the cell cycle by dramatically decreasing DNA synthesis. Notably, HAMNO also attenuates the progression of G2-phase cells into mitosis by a mechanism that remains to be elucidated. Furthermore, HAMNO increases the fraction of chromatin-bound RPA in S-phase but not in G2-phase cells and suppresses DSB repair by homologous recombination. Despite these marked effects on the cell cycle and the DNA damage response, radiosensitization could neither be detected in exponentially growing cultures, nor in cultures enriched in G2-phase cells. Our results complement existing data on RPA inhibitors, specifically HAMNO, and suggest that their antitumor activity by replication stress induction may not extend to radiosensitization. However, it may render cells more vulnerable to other forms of DNA damaging agents through synthetically lethal interactions, which requires further investigation.

Optimized extraction of polysaccharide from Pinus elliottii: Characterization, antioxidant, and moisture-preserving activities.

The study on the extraction conditions, purification, and biological activity of slash pine (Pinus elliottii.) is important for the development of slash pine resources. The optimal process conditions for the extraction of slash pine polysaccharide (SPP) were determined, resulting in a liquid-solid ratio of 66.94 mL/g, extraction temperature of 83.74 °C and extraction time of 2.56 h by using the response surface methodology, and the yield of SPP was 5.99% under the optimized conditions. Following the purification of SPP, the SPP-2 component was obtained and its physicochemical properties, functional group composition, antioxidant capacity, and moisturizing capacity were determined. Structural analysis suggested that SPP-2 has a molecular weight of 118.407 kDa, and was composed of rhamnose, arabinose, fucose, xylose, mannose, glucose, and galactose in a ratio of 5.98: 14.34: 1: 1.75: 13.50: 3.43: 15.79. The antioxidant activity analysis showed that SPP-2 has good free radical scavenging activity, and it was also found to have in vitro moisturizing activity and low irritation. These results suggest that SPP-2 has the potential for applications in the pharmaceutical, food, and cosmetic industries.

Low CDK Activity and Enhanced Degradation by APC/CCDH1 Abolishes CtIP Activity and Alt-EJ in Quiescent Cells.

Alt-EJ is an error-prone DNA double-strand break (DSBs) repair pathway coming to the fore when first-line repair pathways, c-NHEJ and HR, are defective or fail. It is thought to benefit from DNA end-resection-a process whereby 3' single-stranded DNA-tails are generated-initiated by the CtIP/MRE11-RAD50-NBS1 (MRN) complex and extended by EXO1 or the BLM/DNA2 complex. The connection between alt-EJ and resection remains incompletely characterized. Alt-EJ depends on the cell cycle phase, is at maximum in G2-phase, substantially reduced in G1-phase and almost undetectable in quiescent, G0-phase cells. The mechanism underpinning this regulation remains uncharacterized. Here, we compare alt-EJ in G1- and G0-phase cells exposed to ionizing radiation (IR) and identify CtIP-dependent resection as the key regulator. Low levels of CtIP in G1-phase cells allow modest resection and alt-EJ, as compared to G2-phase cells. Strikingly, CtIP is undetectable in G0-phase cells owing to APC/C-mediated degradation. The suppression of CtIP degradation with bortezomib or CDH1-depletion rescues CtIP and alt-EJ in G0-phase cells. CtIP activation in G0-phase cells also requires CDK-dependent phosphorylation by any available CDK but is restricted to CDK4/6 at the early stages of the normal cell cycle. We suggest that suppression of mutagenic alt-EJ in G0-phase is a mechanism by which cells of higher eukaryotes maintain genomic stability in a large fraction of non-cycling cells in their organisms.

Bioplastic production in terms of life cycle assessment: A state-of-the-art review.

The current transition to sustainability and the circular economy can be viewed as a socio-technical response to environmental impacts and the need to enhance the overall performance of the linear production and consumption paradigm. The concept of biowaste refineries as a feasible alternative to petroleum refineries has gained popularity. Biowaste has become an important raw material source for developing bioproducts and biofuels. Therefore, effective environmental biowaste management systems for the production of bioproducts and biofuels are crucial and can be employed as pillars of a circular economy. Bioplastics, typically plastics manufactured from bio-based polymers, stand to contribute to more sustainable commercial plastic life cycles as part of a circular economy in which virgin polymers are made from renewable or recycled raw materials. Various frameworks and strategies are utilized to model and illustrate additional patterns in fossil fuel and bioplastic feedstock prices for various governments' long-term policies. This review paper highlights the harmful impacts of fossil-based plastic on the environment and human health, as well as the mass need for eco-friendly alternatives such as biodegradable bioplastics. Utilizing new types of bioplastics derived from renewable resources (e.g., biowastes, agricultural wastes, or microalgae) and choosing the appropriate end-of-life option (e.g., anaerobic digestion) may be the right direction to ensure the sustainability of bioplastic production. Clear regulation and financial incentives are still required to scale from niche polymers to large-scale bioplastic market applications with a truly sustainable impact.

Trends in using of antihypertensive medication among US CKD adults, NHANES 2001-2018.

Objective: Blood pressure (BP) control rates among adult patients taking antihypertensive medications in the United States have not improved over the last decade. Many CKD adults require more than one class of antihypertensive agent to reach the BP target recommended by the guidelines. However, no study has quantified the proportion of adult CKD patients taking antihypertensive medication who are on monotherapy or combination therapy. Methods: National Health and Nutrition Examination Survey data during 2001-2018 was used, including adults with CKD taking antihypertensive medication (age ≥ 20 years, n = 4,453). BP control rates were investigated under the BP targets recommended by the 2021 KDIGO, the 2012 KDIGO, and the 2017 ACC/AHA guidelines. Results: The percentages of uncontrolled BP among US adults with CKD taking antihypertensive medication were 81.4% in 2001-2006 and 78.2% in 2013-2018. The proportion of monotherapy of antihypertensive regimen were 38.6, 33.3, and 34.6% from 2001 to 2006, 2007-2012, and 2013-2018, with no obvious difference. Similarly, there was no significant change in percentages of dual-therapy, triple-therapy, and quadruple-therapy. Although proportion of CKD adults not treated with ACEi/ARB decreased from 43.5% in 2001-2006 to 32.7% in 2013-2018, treatment of ACEi/ARB among patients with ACR > 300 mg/g had no significant change. Conclusion: The BP control rates among US adult CKD patients taking antihypertensive medications have not improved from 2001 to 2018. Mono-therapy accounted for about one third of adult CKD patients taking antihypertensive medication and not changed. Increasing antihypertensive medication combination therapy may help improve BP control in CKD adults in the United States.

Enhancing Biochar-Based Nonradical Persulfate Activation Using Data-Driven Techniques.

Converting biomass into biochar (BC) as a functional biocatalyst to accelerate persulfate activation for water remediation has attracted much attention. However, due to the complex structure of BC and the difficulty in identifying the intrinsic active sites, it is essential to understand the link between various properties of BC and the corresponding mechanisms promoting nonradicals. Machine learning (ML) recently demonstrated significant potential for material design and property enhancement to help tackle this problem. Herein, ML techniques were applied to guide the rational design of BC for the targeted acceleration of nonradical pathways. The results showed a high specific surface area, and O% values can significantly enhance nonradical contribution. Furthermore, the two features can be regulated by simultaneously tuning the temperatures and biomass precursors for efficient directed nonradical degradation. Finally, two nonradical-enhanced BCs with different active sites were prepared based on the ML results. This work serves as a proof of concept for applying ML in the synthesis of tailored BC for persulfate activation, thereby revealing the remarkable capability of ML for accelerating bio-based catalyst development.

Direct Z-scheme heterojunction Bi/Bi2S3/α-MoO3 photoelectrocatalytic degradation of tetracycline under visible light.

A hot research topic in visible-light-driven photoelectrocatalytic (PEC) oxidation technology is the development of superior photoanode materials. The design of the photoanode system with a direct Z-scheme charge transfer mechanism is crucial to achieving effective charge separation for sustainable photoelectrocatalysis. Here, a novel Bi/Bi2S3/α-MoO3 heterostructure was successfully assembled by a simple and feasible strategy. The direct Z-scheme heterogeneous formed between Bi2S3 and α-MoO3 has the advantages of low resistance, high optical response current and the surface plasmon resonance (SPR) effect of Bi nanoparticles (Bi NPs). Thus, the efficiency of photogenerated carrier separation and transfer is further enhanced, and the catalytic activity is significantly improved. It is impressive that the unique photoanode has achieved a maximum removal efficiency of 85.8% of tetracycline (TC) pollutants under visible light irradiation within 60 min and has excellent stability, which is expected to degrade antibiotics efficiently and environmentally in harsh environments. These characteristics give Bi/Bi2S3/α-MoO3 promising candidates for practical applications in antibiotic degradation.

Bioresource Upgrade for Sustainable Energy, Environment, and Biomedicine.

We conceptualize bioresource upgrade for sustainable energy, environment, and biomedicine with a focus on circular economy, sustainability, and carbon neutrality using high availability and low utilization biomass (HALUB). We acme energy-efficient technologies for sustainable energy and material recovery and applications. The technologies of thermochemical conversion (TC), biochemical conversion (BC), electrochemical conversion (EC), and photochemical conversion (PTC) are summarized for HALUB. Microalgal biomass could contribute to a biofuel HHV of 35.72 MJ Kg-1 and total benefit of 749 $/ton biomass via TC. Specific surface area of biochar reached 3000 m2 g-1 via pyrolytic carbonization of waste bean dregs. Lignocellulosic biomass can be effectively converted into bio-stimulants and biofertilizers via BC with a high conversion efficiency of more than 90%. Besides, lignocellulosic biomass can contribute to a current density of 672 mA m-2 via EC. Bioresource can be 100% selectively synthesized via electrocatalysis through EC and PTC. Machine learning, techno-economic analysis, and life cycle analysis are essential to various upgrading approaches of HALUB. Sustainable biomaterials, sustainable living materials and technologies for biomedical and multifunctional applications like nano-catalysis, microfluidic and micro/nanomotors beyond are also highlighted. New techniques and systems for the complete conversion and utilization of HALUB for new energy and materials are further discussed.

Comparative study of the genomic landscape and tumor microenvironment among large cell carcinoma of the lung, large cell neuroendocrine of the lung, and small cell lung cancer.

Deciphering the genomic profiles and tumor microenvironment (TME) in large cell carcinomas of the lung (LCC), large cell neuroendocrine of the lung (LCNEC), and small cell lung cancer (SCLC) might contribute to a better understanding of lung cancer and then improve outcomes. Ten LCC patients, 12 LCNEC patients, and 18 SCLC patients were enrolled. Targeted next-generation sequencing was used to investigate the genomic profiles of LCC, LCNEC, and SCLC. Tumor-infiltrating lymphocytes (TILs) within cancer cell nests and in cancer stroma were counted separately. Precise 60% of LCNEC patients harbored classical non-small cell lung cancer driver alterations, occurring in BRAF, KRAS, ROS1, and RET. More than 70% of SCLC patients harbored TP53-RB1 co-alterations. Moreover, 88.9%, 40%, and 77.8% of LCC, LCNEC, and SCLC cases had a high tumor mutation burden level with more than 7 mutations/Mb. Furthermore, high index of CD68+ CD163+ (TILs within cancer cell nests/ TILs within cancer cell nests and in cancer stroma, P = .041, 548 days vs not reached) and CD163+ TILs (P = .041, 548 days vs not reached) predicted a shorter OS in SCLC. Our findings revealed the distinct genomic profiles and TME contexture among LCC, LCNEC, and SCLC. Our findings suggest that stratifying LCNEC/SCLC patients based on TME contexture might help clinical disease management.

Membrane-coated nanoparticles for direct recognition by T cells.

The direct modulation of T cell responses is an emerging therapeutic strategy with the potential to modulate undesired immune responses including, autoimmune disease, and allogeneic cells transplantation. We have previously demonstrated that poly(lactide-co-glycolide) particles were able to modulate T cell responses indirectly through antigen-presenting cells (APCs). In this report, we investigated the design of nanoparticles that can directly interact and modulate T cells by coating the membranes from APCs onto nanoparticles to form membrane-coated nanoparticles (MCNPs). Proteins within the membranes of the APCs, such as Major Histocompatibility Complex class II and co-stimulatory factors, were effectively transferred to the MCNP. Using alloreactive T cell models, MCNP derived from allogeneic dendritic cells were able to stimulate proliferation, which was not observed with membranes from syngeneic dendritic cells and influenced cytokine secretion. Furthermore, we investigated the engineering of the membranes either on the dendritic cells or postfabrication of MCNP. Engineered membranes could be to promote antigen-specific responses, to differentially activate T cells, or to directly induce apoptosis. Collectively, MCNPs represent a tunable platform that can directly interact with and modulate T cell responses.

Clean Household Energy Consumption and Residents' Well-Being: Empirical Analysis and Mechanism Test.

Improving well-being is a critical problem for worldwide social progress. Research on well-being effects of clean household energy consumption is of great significance for the realization of United Nation's Sustainable Development Goals (SDGs). Due to the multifaceted role of clean household energy in enhancing well-being as a sustainable development goal, it has attracted extensive academic attention and research but still needs to be further refined through new perspectives. This paper uses data from the 2018 China Health and Retirement Longitudinal Study to conduct an empirical analysis of clean household energy consumption and residents' well-being using an ordered probit model, the instrumental variables method, a conditional mixed process (CMP) method, and a mechanism analysis model. The results show that (1) household clean energy consumption contributes to residents' well-being, and the results remained significant after selecting "Do you have an electric Bicycle?" as an instrumental variable to address the endogeneity question. (2) According to heterogeneity research, women's wellbeing is impacted by clean energy consumption in the household more than men's. Only in rural locations can clean household energy consumption significantly boost well-being; urban and suburban areas are unaffected. (3) In the mechanism analysis, health condition and depression played a mediating role on the impact of clean household energy consumption on well-being, and social contact played a moderating role on the impact of clean household energy consumption on well-being. The findings and policy recommendations in this paper are suggestive of how we can improve the well-being of residents in low- and middle-income countries and provide reference values for research in related fields around the world.

PTEN Loss Enhances Error-Prone DSB Processing and Tumor Cell Radiosensitivity by Suppressing RAD51 Expression and Homologous Recombination.

PTEN has been implicated in the repair of DNA double-strand breaks (DSBs), particularly through homologous recombination (HR). However, other data fail to demonstrate a direct role of PTEN in DSB repair. Therefore, here, we report experiments designed to further investigate the role of PTEN in DSB repair. We emphasize the consequences of PTEN loss in the engagement of the four DSB repair pathways-classical non-homologous end-joining (c-NHEJ), HR, alternative end-joining (alt-EJ) and single strand annealing (SSA)-and analyze the resulting dynamic changes in their utilization. We quantitate the effect of PTEN knockdown on cell radiosensitivity to killing, as well as checkpoint responses in normal and tumor cell lines. We find that disruption of PTEN sensitizes cells to ionizing radiation (IR). This radiosensitization is associated with a reduction in RAD51 expression that compromises HR and causes a marked increase in SSA engagement, an error-prone DSB repair pathway, while alt-EJ and c-NHEJ remain unchanged after PTEN knockdown. The G2-checkpoint is partially suppressed after PTEN knockdown, corroborating the associated HR suppression. Notably, PTEN deficiency radiosensitizes cells to PARP inhibitors, Olaparib and BMN673. The results show the crucial role of PTEN in DSB repair and show a molecular link between PTEN and HR through the regulation of RAD51 expression. The expected benefit from combination treatment with Olaparib or BMN673 and IR shows that PTEN status may also be useful for patient stratification in clinical treatment protocols combining IR with PARP inhibitors.

Data-driven analysis on immobilized microalgae system: New upgrading trends for microalgal wastewater treatment.

Microalgal immobilization is receiving increasing attention as one of the most viable alternatives for upgrading conventional wastewater treatment. However, an in-depth discussion of the state-of-the-art and limitations of available technologies is currently lacking. More importantly, the reason for the hesitant development of immobilized microalgae for wastewater treatment remains unclear, which hinders its practical application. Thus, comprehensively understanding and evaluating details on immobilized microalgae is urgently needed, especially for the current advances of immobilization of microalgae in wastewater treatment over the last few decades. In this review, scientometric approach is used to explore research hotspots and visualize emerging trends. Data-driven analysis is used to scientifically and methodically determine hotspots in the current research on immobilized microalgal wastewater treatment, along with that the implicit inner connection underlying the frequent co-occurring terms was explored in depth. Four hotspots focusing on immobilized microalgae for wastewater treatment were identified, mainly demonstrating: (1) main factors including light, temperature and immobilization methods would majorly affect the treatment performance of immobilized microalgae; (2) immobilized microalgae membrane bioreactor, immobilized microalgae-based microbial fuel cell and immobilized microalgae-based bed reactor are three dominant treatment systems; (3) immobilized microalgae have a higher robustness and tolerance for treating various types of wastewater; and (4) a complete sustainable circle from wastewater treatment to resource conversion via the immobilized microalgae can be achieved. Finally, several new directions and new perspectives that expose the necessity for fulfilling further research and fundamental gaps are pointed out. Taken together, this review provides helpful information to facilitate the development of innovative and feasible immobilized microalgal technologies thus increasing their viability and sustainability.

Remediation technology towards zero plastic pollution: Recent advance and perspectives.

The rapid growth of plastic wastes exceeds efforts to eliminate plastic pollution owing to the outbreak of COVID-19 in 2020 and then aggravates inherent environmental threats to the ecosystem. The paper provided a short introduction relating to the hazards of plastic wastes on environment and a detailed statement about plastic toxicity on human. The article stated on plastic how to enter the body and cause harm for us step by step. Given the toxicity and harm of plastic wastes on human, the degradation of plastic wastes via the physical, chemical and biotic methodologies is looked back. The advanced physical techniques are introduced briefly at firstly. Additionally, evaluate on chemical method for plastic decomposition and review on biotic degradation of plastic. The reactive oxygen species and the enzymes play a crucial role in chemical and biotic degradation processes, respectively. The reactive oxygen species are derived from the activated state of oxides, and the enzymes that aid the microorganism to ingest plastic through its metabolic mechanism are secreted by the microorganism. Subsequently, the potential possibility of upcycling plastic is analyzed from two aspects of the technology and application. The innovative technology utilizes sunlight as driver-power of plastic upcycling. And the carbon capture, utilization and sequestration and the growth substrate provided the novel guided directions for plastic recycle. Lastly, the three suggestions on plastic waste management are expected to establish an economy and efficient plastic sorting system, and two engineering solutions on plastic recycle are to make a contribution for sustainable upcycling of plastic.

Biowastes for biodegradable bioplastics production and end-of-life scenarios in circular bioeconomy and biorefinery concept.

Due to global urbanization, industrialization, and economic development, biowastes generation represents negative consequences on the environment and human health. The use of generated biowastes as a feedstock for biodegradable bioplastic production has opened a new avenue for environmental sustainability from the circular (bio)economy standpoint. Biodegradable bioplastic production can contribute to the sustainability pillars (environmental, economic, and social). Furthermore, bioenergy, biomass, and biopolymers production after recycling of biodegradable bioplastic can help to maintain the energy-environment balance. Several types of biodegradable bioplastic, such as starch-based, polyhydroxyalkanoates, polylactic acid, and polybutylene adipate terephthalate, can achieve this aim. In this review, an overview of the main biowastes valorization routes and the main biodegradable bioplastic types of production, application, and biodegradability are discussed to achieve the transition to the circular economy. Additionally, end-of-life scenarios (up-cycle and down-cycle) are reviewed to attain the maximum environmental, social, and economic benefit from biodegradable bioplastic products under biorefinery concept.

Household clean energy consumption and health: Theoretical and empirical analysis.

The impact of energy consumption on health has become a widely debated topic around the world. However, much of the current research on this topic lacks a theoretical basis. As a result, this paper employs both theoretical and empirical analysis to investigate the impact of household clean energy consumption on residents' health. First, based on the theories of health economics and energy economics, this paper believes that the usage of clean energy can improve the health of residents. Then, the sample for this study is comprised of data from the 2018 China Health and Retirement Longitudinal Study, and the Order Probit Model is applied for the empirical analysis. The outcomes of basic regression, robustness testing, and the treatment of endogenous factors reveal that the usage of clean energy has greatly benefited the health of residents. Furthermore, the heterogeneity analysis shows that long-term use of clean energy greatly improved the health of non-religious people and had a more pronounced impact on the health of women and low-income residents. In addition, the mechanistic analysis indicates that subjective happiness and air quality played a partial mediating role in the impact of cleaner energy consumption on health. Finally, cleaner household energy reduced the prevalence of hypertension, hyperlipidemia, lung disease, asthma, and depression. The conclusion of this paper supports the view of some existing literature, and several policy recommendations are made based on the research findings.

Does Cleaner Household Energy Promote Agricultural Green Production? Evidence from China.

Cleaner household energy for agricultural green production can significantly alleviate energy poverty and food security, thus contributing to global sustainable development. Using survey micro-data collected from Sichuan Province, the ordered probit model, OLS model, and instrumental variables approach were applied for empirical analysis. The results show that: (1) cleaner household energy significantly enhances farmer's agricultural green production awareness and improves agricultural green production levels, which is still significant after treating endogenous issues with the conditional mixing process estimation method and 2SLS model; (2) health plays a partially mediating effect of cleaner household energy on agricultural green production awareness and agricultural green production levels; (3) environmental protection awareness and digital literacy have a moderating effect and reinforce the positive impact of cleaner household energy on agricultural green production awareness and agricultural green production levels. This research suggests that governments can enhance the impact of cleaner household energy on agricultural green production through price and subsidy mechanisms.