RESUMO
OBJECTIVE: To study the correlation between the GP78 gene polymorphism and blood fat, blood glucose, blood pressure and coronary atherosclerotic heart disease. METHODS: A total of 72 patients with coronary atherosclerotic heart disease were selected as the observation group, and 68 healthy participants were selected as the control group. The gp78 gene polymorphism of both groups was studied via polymerase chain reaction-restriction fragment length polymorphism (RFLP). At the same time, the multiple expression quantities of the GP78 gene in the tissues of both groups were tested via fluorogenic quantitative PCR, enzyme-linked immunosorbent assay (ELISA) and Western-blotting assay. Furthermore, the blood fat, blood glucose and blood pressure of subjects in both groups were tested. RESULTS: The percentages of the gp78 gene polymorphisms of Arg/Arg, Arg/Gly and Gly/Gly at the 145 locus of the study subjects in the observation group were 12.3%, 43.2% and 44.5%, respectively, while those in the control group were 74.3%, 11.2% and 14.5%, respectively, and there were significant differences between both groups. Based on the test results of the blood fat, blood glucose and blood pressure of the objects in the observation group and control group, significant differences were found between the two groups (P<0.05). CONCLUSION: There was a significant correlation between the 145 locus of the gp89 gene and coronary atherosclerotic heart disease, indexes of blood fat, blood glucose and blood pressure.
Assuntos
Aterosclerose/genética , Doença da Artéria Coronariana/genética , DNA/genética , Polimorfismo Genético , Receptores do Fator Autócrino de Motilidade/genética , Adulto , Aterosclerose/metabolismo , Western Blotting , Doença da Artéria Coronariana/metabolismo , Ensaio de Imunoadsorção Enzimática , Feminino , Seguimentos , Genótipo , Humanos , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Receptores do Fator Autócrino de Motilidade/metabolismo , Estudos RetrospectivosRESUMO
The study of public health history, bound to follow the development of public health facilities, is an important branch among all the studies of western social history of medicine. The development of public health history has experienced a course from optimism to objectivity as public health facilities. The study of public health history embraces three major aspects, hygiene, social welfare and philanthropy, those all have a distinct sociality and reality.