3D free-assembly modular microfluidics inspired by movable type printing.

Reconfigurable modular microfluidics presents an opportunity for flexibly constructing prototypes of advanced microfluidic systems. Nevertheless, the strategy of directly integrating modules cannot easily fulfill the requirements of common applications, e.g., the incorporation of materials with biochemical compatibility and optical transparency and the execution of small batch production of disposable chips for laboratory trials and initial tests. Here, we propose a manufacturing scheme inspired by the movable type printing technique to realize 3D free-assembly modular microfluidics. Double-layer 3D microfluidic structures can be produced by replicating the assembled molds. A library of modularized molds is presented for flow control, droplet generation and manipulation and cell trapping and coculture. In addition, a variety of modularized attachments, including valves, light sources and microscopic cameras, have been developed with the capability to be mounted onto chips on demand. Microfluidic systems, including those for concentration gradient generation, droplet-based microfluidics, cell trapping and drug screening, are demonstrated. This scheme enables rapid prototyping of microfluidic systems and construction of on-chip research platforms, with the intent of achieving high efficiency of proof-of-concept tests and small batch manufacturing.

Reconstruction algorithm using 2N+1 raw images for structured illumination microscopy.

This paper presents a structured illumination microscopy (SIM) reconstruction algorithm that allows the reconstruction of super-resolved images with 2N + 1 raw intensity images, with N being the number of structured illumination directions used. The intensity images are recorded after using a 2D grating for the projection fringe and a spatial light modulator to select two orthogonal fringe orientations and perform phase shifting. Super-resolution images can be reconstructed from the five intensity images, enhancing the imaging speed and reducing the photobleaching by 17%, compared to conventional two-direction and three-step phase-shifting SIM. We believe the proposed technique will be further developed and widely applied in many fields.

Efficient Nd:YAG crystal laser with rotatory pumping.

The performance of solid-state laser is limited by the thermal effects in the gain medium. In this study, we investigated the implementation of an efficient short-cavity continuous-wave 1064 nm Nd:YAG solid-state laser by using a rotatory pumping scheme to alleviate the thermal accumulation in the gain medium. With this method, the laser power reached 25.7 W with a slope efficiency of 41.5% at a 10.0-mm pump rotation radius and an optimum rotation rate of 2300 rpm. The influence of rotatory pumping radius and rotation rates was analyzed, and the results indicated that the rotatory pumping would be promising technique for the power scaling of solid-state lasers.

Large-field lattice structured illumination microscopy.

In this paper, we present large-field, five-step lattice structured illumination microscopy (Lattice SIM). This method utilizes a 2D grating for lattice projection and a spatial light modulator (SLM) for phase shifting. Five phase-shifted intensity images are recorded to reconstruct a super-resolution image, enhancing the imaging speed and reducing the photo-bleaching both by 17%, compared to conventional two-direction and three-shift SIM. Furthermore, lattice SIM has a three-fold spatial bandwidth product (SBP) enhancement compared to SLM/DMD-based SIM, of which the fringe number is limited by the SLM/DMD pixel number. We believe that the proposed technique will be further developed and widely applied in many fields.

Phase image correlation spectroscopy for detecting microfluidic dynamics.

It is essential to quantify the physical properties and the dynamics of flowing particles in many fields, especially in microfluidic-related applications. We propose phase image correlation spectroscopy (PICS) as a versatile tool to quantify the concentration, hydro-diameter, and flow velocity of unlabeled particles by correlating the pixels of the phase images taken on flowing particles in a microfluidic device. Compared with conventional image correlation spectroscopy, PICS is minimally invasive, relatively simple, and more efficient, since it utilizes the intrinsic phase of the particles to provide a contrast instead of fluorescent labeling. We demonstrate the feasibility of PICS by measuring flowing polymethylmethacrylate (PMMA) microspheres and yeast in a microfluidic device. We can envisage that PICS will become an essential inspection tool in biomedicine and industry.

Feature recognition of a 2D array vortex interferogram using a convolutional neural network.

A vortex array has important applications in scenarios where multiple vortex elements with the same or different topological charges are required simultaneously. Therefore, the detection of the vortex array is vital. Here, the interferogram between the off-axis Walsh-phase plate and the vortex array is first obtained and then decoded through a convolution neural network (CNN), which can simultaneously determine the topological charge, chirality, and the initial angle. Both the theory and experiment prove that a CNN has a remarkable effect on the classification and detection of vortex arrays.

Generation of vector polarization in a Nd:YAG laser.

We demonstrated the generation of an azimuthally and radially polarized laser beam in a Nd:YAG laser in which a birefringent yttrium vanadate (c-cut YVO4) crystal was used as the intra-cavity polarization discriminator. AP and RP with respective output 2.4W (o-o efficiency of 35.4%, M2 = 2.3) and 2.52W (o-o efficiency of 37.2%, M2 = 2.4) were generated at absorbed pump power 6.78W. We discuss a simple method for converting between azimuthal and radial polarizations by only regulating input pump power and mechanism of mode selection in the laser. This vector laser will facilitate many applications.

High-power, efficient and azimuthally polarized ytterbium-doped fiber laser.

An ytterbium-doped fiber laser was demonstrated to emit azimuthally polarized light with high laser power and efficiency, in which a birefringent yttrium vanadate (YVO(4)) crystal was used as the intracavity polarization discriminator. The laser power reached 3.94 W with a slope efficiency of ∼71%. Our study proved that an efficient, high-power vector fiber laser would be realistic under the utilization of a simple and low-cost birefringent crystal as polarization discriminator.

Analytical solution of the transient temperature profile in gain medium of passively Q-switched microchip laser.

The transient temperature evolution in the gain medium of a continuous wave (CW) end-pumped passively Q-switched microchip (PQSM) laser is analyzed. By approximating the time-dependent population inversion density as a sawtooth function of time and treating the time-dependent pump absorption of a CW end-pumped PQSM laser as the superposition of an infinite series of short pumping pulses, the analytical expressions of transient temperature evolution and distribution in the gain medium for four- and three-level laser systems, respectively, are given. These analytical solutions are applied to evaluate the transient temperature evolution and distribution in the gain medium of CW end-pumped PQSM Nd:YAG and Yb:YAG lasers.

2 × 2 arrayed and passively Q-switched Nd:YVO4 laser under Dammann-arrayed pumping.

We reported our proof-of-concept investigation in developing an arrayed and passively Q-switched Nd:YVO4 laser. In the investigation, we used a Dammann grating to diffract the pumping light from a 808 nm laser diode into 2×2 pump beam array, and then used this pump array to pump a monolithic Nd:YVO4 laser crystal standing with a Cr4+:YAG saturable absorber inside a simple plano-plano resonator. As a result, 2×2 arrayed and nanosecond laser pulses were emitted. The laser-diode-pumped monolithic solid-state laser incorporated with a Dammann grating is promising for application in high-peak-power and multipoint pulse ignition of an automobile engine.

Radially polarized and passively Q-switched fiber laser.

We report, for the first time to our knowledge, a radially polarized and passively Q-switched Yb-doped fiber laser. By using a Cr(4+):YAG crystal as a saturable absorber and a photonic crystal grating as a polarization mirror, a radially polarized pulse is produced, which has 202 W of peak power, 75 ns duration, and ~92% polarization purity at a 56.6 kHz repetition rate. The Q-switched pulse with radial polarization from the fiber laser would facilitate numerous applications.

Efficient, high-power, and radially polarized fiber laser.

We demonstrate an ytterbium-doped fiber laser that emits high-power radially polarized light efficiently. In this study, a photonic crystal grating (PCG) was used as a polarization-selective output coupler, and the power of the radially polarized laser reached 2.42 W with a slope efficiency of 45.9% and a polarization purity of 96%. The results reveal that the inclusion of the PCG mirror into the fiber laser are particularly promising for generating high-power radially polarized light efficiently in view of its many important applications.

Radially polarized and pulsed output from passively Q-switched Nd:YAG ceramic microchip laser.

For the first time, to the best of our knowledge, a radially polarized laser pulse was produced from a passively Q-switched Nd:YAG ceramic microchip laser with a piece of Cr4+:YAG crystal as the saturable absorber and multilayer concentric subwavelength grating as the polarization-selective output coupler. The averaged laser power reached 450 mW with a slope efficiency of 30.2%. The laser pulse had a maximum peak power of 759 W, a minimum pulse duration of 86 ns, and a 6.7 kHz repetition rate at 3.7 W absorbed pump power. The polarization degree of the radially polarized pulse was measured to be as high as 97.4%. Such a radially polarized laser pulse with a high peak power and a short width is important to numerous applications such as metal cutting.

Efficient excitations of radially and azimuthally polarized Nd3+:YAG ceramic microchip laser by use of subwavelength multilayer concentric gratings composed of Nb2O5/SiO2.

Cylindrical vector beams were produced from laser diode end-pumped Nd:YAG ceramic microchip laser by use of two types of subwavelength multilayer gratings as the axisymmetric-polarization output couplers respectively. The grating mirrors are composed of high- and low-refractive- index (Nb(2)O(5)/SiO(2)) layers alternately while each layer is shaped into triangle and concentric corrugations. For radially polarized laser output, the beam power reached 610mW with a polarization extinction ratio (PER) of 61:1 and a slope efficiency of 68.2%; for azimuthally polarized laser output, the beam power reached 626mW with a PER of 58:1 and a slope efficiency of 47.6%. In both cases, the laser beams had near-diffraction limited quality. Small differences of beam power, PER and slope efficiency between radially and azimuthally polarized laser outputs were not critical, and could be minimized by further optimized adjustment to laser cavity and the reflectances of respective grating mirrors. The results manifested, by use of the photonic crystal gratings mirrors and end-pumped microchip laser configuration, CVBs can be generated efficiently with high modal symmetry and polarization purity.

Converging-axicon-based radially polarized ytterbium fiber laser and evidence on the mode profile inside the gain fiber.

The radially polarized mode is achieved from an active Yb fiber by utilizing of an intracavity converging axicon, where the axicon acts as a TM(01) mode selector based on not only its Brewster convex surface but also the distance between its vertex and plane output coupler. The polarization state of the annular laser beam is checked by using a home-made eight-hole aperture. Furthermore, an uncoated plane glass plate is inserted into the cavity, and the reflected beam points to the existence of an annular lasing mode inside the gain fiber. The issues for developing high-power radially polarized fiber lasers also are considered.

Generation of radially polarized mode in Yb fiber laser by using a dual conical prism.

For the first time to our knowledge, a radially polarized beam is generated in an Yb-doped multimode double-clad fiber laser by using an intracavity dual conical prism. Up to 6.2 mW of output power is obtained from a 2 m long gain fiber with 7.4% slope efficiency. This research opens a new window to obtaining a radially polarized beam directly from an active fiber.

Maximum value of the pulse energy of a passively Q-switched laser as a function of the pump power.

The finite recovery time Ts of the bleached absorber is presented as one of the possible mechanisms accounting for the increase-maximum-decrease in pulse energy E with the pumping rate Wp in cw-pumped passively Q-switched solid-state lasers, by analytically evaluating the sign of the derivative partial differentialE/ partial differentialWP. The results show that, in the low pump regime (T>Ts, T is the interpulse period), the initial population density ni remains constant, the final population density nf decreases with Wp, and this results in a monotonic increase of E with Wp. In the high pump regime (T

Residual pump light as a probe of self-pulsing instability in an ytterbium-doped fiber laser.

Self-pulsing instability in the ytterbium-doped fiber (YDF) laser is investigated with the pump-bypassed cavity configuration. The residual pump light acts as a probe of the intracavity dynamics, and the temporal behavior of the light shows correlations with the self-mode locking instability in the original cavity and the sustained self-pulsing instability in the modified cavity of the YDF laser. The results suggest that the interactions among stimulated emission, pump absorption and/or reabsorption could account for self-pulsing instability in the YDF laser. The pump-bypassed laser cavity configuration can be used to investigate the instabilities of various kinds of fiber lasers.