RESUMO
Allergen immunotherapy (AIT) is the only curative treatment for allergic diseases. However, AIT has many disadvantages related to efficiency, safety, long-term duration, and patient compliance. Dendritic cells (DCs) have an important role in antigen-specific tolerance induction; thus, DC-targeting strategies to treat allergies such as glutaraldehyde crosslinked antigen to mannoprotein (MAN) have been established. However, glutaraldehyde crosslinking may reduce the antigen presentation efficiency of DCs. To overcome this, we developed a MAN-coated ovalbumin (OVA) nanoparticle (MDO), which uses intermolecular disulfide bond to crosslink OVA and MAN. MDO effectively targeted DCs resulting in tolerogenic DCs, and promoted higher antigen presentation efficiency by DCs compared with OVA or glutaraldehyde crosslinked nanoparticles. In vitro and in vivo experiments showed that DCs exposed to MDO induced Treg cells. Moreover, MDO had low reactivity with anti-OVA antibodies and did not induce anaphylaxis in allergic mice, demonstrating its high safety profile. In a mouse model of allergic asthma, MDO had significant preventative and therapeutic effects when administered orally or subcutaneously. Therefore, MDO represents a promising new approach for the efficient and safe treatment of allergies.
Assuntos
Hipersensibilidade , Nanopartículas , Humanos , Camundongos , Animais , Mananas , Glutaral , Células Dendríticas , Alérgenos , Dessensibilização Imunológica , Nanopartículas/química , Ovalbumina , Imunoterapia/métodosRESUMO
In view of the characteristics and risks of ammonia, its removal is important for industrial production and environmental safety. In this study, viscose-based activated carbon fiber (ACF) was used as a substrate and chemically modified by nitric acid impregnation to enhance the adsorption capacity of the adsorbent for ammonia. A series of modified ACF-based adsorbents were prepared and characterized using BET, FTIR, XPS, and Boehm titration. Isotherm tests (293.15 K, 303.15 K, 313.15 K) and dynamic adsorption experiments were performed. The characterization results showed that impregnation with low concentrations of nitric acid not only increased the surface acidic functional group content but also increased the specific surface area, while impregnation with high concentrations of nitric acid could be able to decrease the specific surface area. ACF-N-6 significantly increased the surface functional group content without destroying the physical structure of the activated carbon fibers. The experimental results showed that the highest adsorption of ammonia by ACFs was 14.08 mmol-L-1 (ACF-N-6) at 293 K, and the adsorption capacity was increased by 165% compared with that of ACF-raw; by fitting the adsorption isotherm and calculating the equivalent heat of adsorption and thermodynamic parameters using the Langmuir-Freundlich model, the adsorption process could be found to exist simultaneously. Regarding physical adsorption and chemical adsorption, the results of the correlation analysis showed that the ammonia adsorption performance was strongly correlated with the carboxyl group content and positively correlated with the relative humidity (RH) of the inlet gas. This study contributes to the development of an efficient ammonia adsorption system with important applications in industrial production and environmental safety.
RESUMO
Nanoparticles (NPs) for allergen immunotherapy have garnered attention for their high efficiency and safety compared with naked antigen proteins. In this work, we present mannan-coated protein NPs, incorporating antigen proteins for antigen-specific tolerance induction. The heat-induced formation of protein NPs is a one-pot preparation method and can be applied to various proteins. Here, the NPs were formed spontaneously via heat denaturation of three component proteins: an antigen protein, human serum albumin (HSA) as a matrix protein, and mannoprotein (MAN) as a targeting ligand for dendritic cells (DCs). HSA is non-immunogenic, therefore suitable as a matrix protein, while MAN coats the surface of the NP. We applied this method to various antigen proteins and found that the self-disperse after heat denaturation was a requirement for incorporation into the NPs. We also established that the NPs could target DCs, and the incorporation of rapamycin into the NPs enhanced the induction of a tolerogenic phenotype of DC. The MAN coating provided steric hindrance and heat denaturation destroyed recognition structures, successfully preventing anti-antigen antibody binding, indicating the NPs may avoid anaphylaxis induction. The MAN-coated NPs proposed here, prepared by a simple method, have the potential for effective and safe allergies treatment for various antigens.
Assuntos
Mananas , Nanopartículas , Humanos , Albumina Sérica Humana , Antígenos/química , Tolerância Imunológica , Nanopartículas/químicaRESUMO
BACKGROUND: Calcific aortic valve disease (CAVD) is the most prevalent valvular disease and has high morbidity and mortality. CAVD is characterized by complex pathophysiological processes, including inflammation-induced osteoblastic differentiation in aortic valve interstitial cells (AVICs). Novel anti-CAVD agents are urgently needed. Protein tyrosine phosphatase nonreceptor type 22 (PTPN22), an intracellular nonreceptor-like protein tyrosine phosphatase, is involved in several chronic inflammatory diseases, including rheumatoid arthritis and diabetes. However, it is unclear whether PTPN22 is involved in the pathogenesis of CAVD. METHODS: We obtained the aortic valve tissue from human and cultured AVICs from aortic valve. We established CAVD mice model by wire injury. Transcriptome sequencing, western bolt, qPCR, and immunofluorescence were performed to elucidate the molecular mechanisms. RESULTS: Here, we determined that PTPN22 expression was upregulated in calcific aortic valve tissue, AVICs treated with osteogenic medium, and a mouse model of CAVD. In vitro, overexpression of PTPN22 induced osteogenic responses, whereas siRNA-mediated PTPN22 knockdown abolished osteogenic responses and mitochondrial stress in the presence of osteogenic medium. In vivo, PTPN22 ablation ameliorated aortic valve lesions in a wire injury-induced CAVD mouse model, validating the pathogenic role of PTPN22 in CAVD. Additionally, we discovered a novel compound, 13-hydroxypiericidin A 10-O-α-D-glucose (1 â 6)-ß-D-glucoside (S18), in a marine-derived Streptomyces strain that bound to PTPN22 with high affinity and acted as a novel inhibitor. Incubation with S18 suppressed osteogenic responses and mitochondrial stress in human AVICs induced by osteogenic medium. In mice with aortic valve injury, S18 administration markedly alleviated aortic valve lesions. CONCLUSION: PTPN22 plays an essential role in the progression of CAVD, and inhibition of PTPN22 with S18 is a novel option for the further development of potent anti-CAVD drugs. Therapeutic inhibition of PTPN22 retards aortic valve calcification through modulating mitochondrial dysfunction in AVICs.
Assuntos
Estenose da Valva Aórtica , Valva Aórtica , Humanos , Animais , Camundongos , Valva Aórtica/metabolismo , Valva Aórtica/patologia , Monoéster Fosfórico Hidrolases , Estenose da Valva Aórtica/tratamento farmacológico , Estenose da Valva Aórtica/genética , Células Cultivadas , Proteína Tirosina Fosfatase não Receptora Tipo 22/metabolismoRESUMO
Fatty-acid binding protein 4 (FABP4) is an essential driver for the progression of metabolic-related inflammatory diseases including obesity, diabetes, atherosclerosis, and various lipid metabolism-related tumors. However, FABP4 inhibitors are not yet available for clinical use, which may be associated with their poor selectivity of FABP3, unsatisfactory efficacy and physicochemical properties. Herein, we reported a systematic optimization of a class of biphenyl scaffold molecules as potent FABP4 inhibitors. Further in vitro and in vivo pharmacokinetic studies identified a selective and orally bioavailable compound 10g, with Ki of 0.51 µM against FABP4, Ki of 33.01 µM against FABP3 and bioavailability F% value of 89.4%. In vivo anti-inflammatory efficacy and multi-organ protection study in LPS-induced inflammatory mice model highlighted the potential of compound 10g as a therapeutic candidate in inflammation-related diseases.
Assuntos
Compostos de Bifenilo , Proteínas de Ligação a Ácido Graxo , Camundongos , Animais , Compostos de Bifenilo/farmacologia , Anti-Inflamatórios/farmacologia , Anti-Inflamatórios/uso terapêutico , Anti-Inflamatórios/metabolismoRESUMO
Higher activity and alkaline α-amylases are desired for textile desizing and detergent additive. Here, rational design was used to improve the specific activity and thermostability of the α-amylase BLA from Bacillus licheniformis. Seventeen mutants of BLA were designed based on sequence consensus analysis and folding free energy calculation, and characterized by measuring their respective activity and thermostability at pH 8.5. Among them, mutant Q360C exhibited nearly threefold improved activity than that of wild-type and retained a higher residual activity (75% vs 59% for wild-type) after preincubation at 70 â for 30 min. The modeled structures and molecular dynamics simulations analysis demonstrated that the enhanced hydrophobic interaction near residue 360 and reduced disturbance to the conformation of catalytic residues are the possible reasons for the improved thermostability and activity of Q360C. The results suggest that 360th of BLA may act as a hotspot for engineering other enzymes in the GH13 superfamily.
Assuntos
alfa-Amilases , alfa-Amilases/genética , alfa-Amilases/química , Estabilidade Enzimática , TemperaturaRESUMO
Clostridioides difficile causes nosocomial antibiotic-associated diarrhea on a global scale. Susceptibility to C. difficile infection (CDI) is influenced by the composition and metabolism of gut microbiota, which in turn are affected by diet. However, the mechanism underlying the interplay between diet and gut microbiota that modulates susceptibility to CDI remains unclear. Here, we show that a soy protein diet increases the mortality of antibiotic-treated, C. difficile-infected mice while also enhancing the intestinal levels of amino acids (aas) and relative abundance of Lactobacillus genus. Indeed, Ligilactobacillus murinus-mediated fermentation of soy protein results in the generation of aas, thereby promoting C. difficile growth, and the process involves the anchored cell wall proteinase PrtP. Thus, mutual interaction between dietary protein and the gut microbiota is a critical factor affecting host susceptibility to CDI, suggesting that dietary protein sources can be an important determinant in controlling the disease.
Assuntos
Clostridioides difficile , Infecções por Clostridium , Microbioma Gastrointestinal , Animais , Antibacterianos , Proteínas Alimentares , Camundongos , Proteínas de SojaRESUMO
Calcific aortic valve disease (CAVD) is a valvular disease frequently in the elderly individuals that can lead to the valve dysfunction. Osteoblastic differentiation of human aortic valve interstitial cells (HAVICs) induced by inflammation play a crucial role in CAVD pathophysiological processes. To date, no effective drugs for CAVD have been established, and new agents are urgently needed. Piericidin glycosides, obtained from a marine-derived Streptomyces strain, were revealed to have regulatory effects on mitochondria in previous studies. Here, we discovered that 13-hydroxypiericidin A 10-O-α-D-glucose (1â6)-ß-D-glucoside (S18), a specific piericidin diglycoside, suppresses lipopolysaccharide- (LPS) induced inflammatory responses of HAVICs by alleviating mitochondrial stress in an interleukin (IL)-37-dependent manner. Knockdown of IL-37 by siRNA not only exaggerated LPS-induced HAVIC inflammation and mitochondrial stress but also abrogated the anti-inflammatory effect of S18 on HAVICs. Moreover, S18 alleviated aortic valve lesions in IL-37 transgenic mice of CAVD model. Microscale thermophoresis (MST) and docking analysis of five piericidin analogues suggested that diglycosides, but not monoglycosides, exert obvious IL-37-binding activity. These results indicate that S18 directly binds to IL-37 to alleviate inflammatory responses in HAVICs and aortic valve lesions in mice. Piericidin diglycoside S18 is a potential therapeutic agent to prevent the development of CAVD.
Assuntos
Estenose da Valva Aórtica , Valva Aórtica , Glicosídeos , Interleucina-1 , Animais , Valva Aórtica/patologia , Calcinose , Células Cultivadas , Glicosídeos/farmacologia , Humanos , Inflamação , Interleucina-1/metabolismo , Interleucinas , Lipopolissacarídeos , CamundongosRESUMO
Aim: Behavior management techniques (BMTs) efficiently deliver dental treatment to children with dental anxiety. The objective of this quasi-experimental study was to examine whether the efficacy of BMTs applied for the improvement of compliance in pediatric patients differs between children 3-10-year-olds from single-child and multi-child families. Materials and Methods: In this quasi-experimental, 197 caregiver-child couples were divided into two groups: single-child group (116 couples) and multi-child group (81 couples). Children's pre- and post-treatment anxiety levels were measured by facial mood scale (FMS) and Frankl Behavior Rating Scale (FBRS), respectively.Caregivers' dental anxiety was measured by the Chinese version of the Modified Dental Anxiety Scale (MDAS), which was included in the self-designed questionnaire. Data were analyzed by using the Mann-Whitney U-test, chi-square tests, and binary multivariate regression analysis. Results: There was no statistically significant difference in the demographic characteristics of the children between the two groups. BMTs were found to be capable of reducing children's dental anxiety (CDA): the compliance rate was 45.69-88.79% in the single-child group and 44.44-85.79% in the multi-child group pre- and post-BMTs, but there was no significant difference in the change of compliance between the two groups (p > 0.05). In the subgroup analysis, parenting style (odds ratio [OR] = 0.054, p < 0.05) and father's education (OR = 8.19, p < 0.05) affected the varies of children's compliance in the single-child group. In contrast, in the multi-child group, gender (OR = 8.004, p < 0.05) and mother's occupation (OR = 0.017, p < 0.05) were associated with these changes in compliance. Conclusions: In this study, BMTs were proved to be beneficial in improving compliance in 3- to 10-year-olds children in dental treatment. Though there was no significant difference in the change of compliance between children from single-child and multi-child families, different associated factors may affect the two groups. Therefore, the related family factors should be taken into account when professionals manage each child's behavior in dental practice.
Assuntos
Comportamento Infantil , Poder Familiar , Terapia Comportamental , Cuidadores , Criança , Humanos , Inquéritos e QuestionáriosRESUMO
Solidification of cathode ray tube (CRT) panel glass was carried out using a hydrothermal processing method. In this way, the glass powder was first compacted in a mold at 20 MPa, and then hydrothermally cured in an autoclave under saturated steam pressure at 200 â for 6 h. The CRT panel glass was then hydrothermally solidified by the formation of tobermorite (Ca5Si6O16(OH)2·4H2O), which was encouraged by the addition of slaked lime (Ca(OH)2). The strength of solidified specimen heavily depended on the amount of tobermorite formed, with higher concentrations of tobermorite producing commensurately greater mechanical strength. With the addition of Ca(OH)2 at 20-30% by mass, the specimen achieved a bending strength of approximately 16 MPa, which was sufficiently great for using as a construction material. As such, there is cause to believe that the hydrothermal processing method used here may have great potential for resource utilization of CRT panel glass, and the performance of the product is suitable for use as building materials.
Assuntos
Tubo de Raio Catódico , Materiais de Construção , VidroRESUMO
In this study, the feasibility of three methods on enhancing the recovery performance of biofilter after the interference and starvation periods was evaluated. Results show that despite the pressure drop risk, supplementation of 7.5% (w/v) Polyethylene glycol-600 (PEG-600) resulted in quick recovery on removal efficiency in both short- and long-term interference shutdown experiments. Tinidazole Tablets (2 mg/L), a Bacteroidetes-specific antibiotic, are more suitable to apply as a one-time shot to improve recovery of biofilter as the second dose of Tinidazole Tablets was no longer effective presumably caused by the increased drug resistance. It is worth noting that the maximum elimination capacity of 134 g/(m3·h) was observed with Pseudomonas putida (P. putida) BRJC1032 addition. The biodegradation kinetic, biological characteristics and microbial community evolution in biofilters were systematically analyzed for finding the suitable methods to enhance recovery performance, which is of great value for the further industrial application of the biofilter technology.
Assuntos
Microbiota , Tolueno , Biodegradação Ambiental , FiltraçãoRESUMO
Intergenerational support is bidirectional, and reverse intergenerational support refers to parents providing financial support, time support, and spiritual support to their offspring. The emergence of reverse intergenerational support has created role conflicts among different groups of older adults. Based on survey data from 3,170 elderly people in eight sample provinces in China, this paper empirically investigates the relationship between reverse intergenerational support and the happiness of the elderly in contemporary China and the moderating effect of role conflict in it, using an ordered logit model. It was found that, first, reverse economic support reduces the happiness of the elderly, and reverse time support and reverse spiritual support can significantly enhance the happiness of the elderly. Second, in the presence of role conflict, the effect of reverse time support and reverse spiritual support on the enhancement of older adults' happiness was suppressed; in the presence of role enhancement, the effect of reverse economic support on the reduction of older adults' happiness was mitigated. The above findings provide new empirical evidence for understanding the relationship between reverse intergenerational support and the happiness of the elderly, which is prevalent in contemporary China, and offer new insights for enhancing happiness.
RESUMO
The fraction of antibiotics that are excreted from the intestine during administration leads to disruption of commensal bacteria as well as resulting in dysbiosis and various diseases. To protect the gut microbiota during treatment with antibiotics, use of activated carbon (AC) has recently been reported as a method to adsorb antibiotics. However, the antibiotic adsorption by AC is nonspecific and may also result in the adsorption of essential biological molecules. In this work, we reported that an anion exchange resin (AER) has better specificity than AC for adsorbing the ß-lactam antibiotic cefoperazone (CEF). Because CEF has a negatively charged carboxylate group and a conjugated system, the AER was used to adsorb CEF through electrostatic and π-π interactions. The AER was specific for CEF over biological molecules such as bile acids and vitamins in the intestine. The AER protected Escherichia coli from CEF in vitro. Furthermore, oral administration of the AER reduced the fecal free CEF concentration, and protected the gut microbiota from CEF-induced dysbiosis.
Assuntos
Microbioma Gastrointestinal , Adsorção , Resinas de Troca Aniônica , Ânions , Antibacterianos , beta-LactamasRESUMO
The Chinese government has developed an ambitious project to promote the application of ethanol gasoline (E10) on a national scale since 2017. Given the difference in fuel properties between E10 and traditional gasoline, it is necessary to evaluate the volatile organic compound (VOC) emissions from E10-fuelled vehicles. In this study, a two-week sampling campaign was conducted in an urban tunnel, in which E10-fuelled vehicles were dominant, to evaluate the characteristics of VOC emissions from the mixed fleet. In total, 105 VOC species were identified, and the ozone formation potential (OFP) and secondary organic aerosol formation potential (SOAFP) were estimated. The results showed that for vehicular VOC concentrations in the tunnel, alkanes, oxygenated VOCs (OVOCs) and alkenes were the most abundant VOC groups, with the average proportion being more than 80% of the total VOCs. The fleet-average VOC emission factor (EF) was 14.8 mg/km/veh, which was much lower than that from traditional gasoline-fuelled vehicle fleets, and alkanes, OVOCs, alkenes and aromatics were the major VOC groups. Because of the large number of E10-fuelled vehicles in the mixed fleet, a high proportion of OVOCs among the vehicular VOC emissions was observed. Ethane, acrolein, ethanol, ethylene and toluene were the top five VOC species with the largest EF in VOC emissions from the fleet. Alkenes were the main contributors with an average contribution of 43.9% of the total OFP, whereas aromatics dominated the total SOAFP by 95.8% on average. These results may provide a reference for the extensive application of ethanol gasoline and the development of vehicular emission models.
Assuntos
Poluentes Atmosféricos , Ozônio , Compostos Orgânicos Voláteis , Aerossóis/análise , Poluentes Atmosféricos/análise , China , Monitoramento Ambiental , Ozônio/análise , Emissões de Veículos/análise , Compostos Orgânicos Voláteis/análiseRESUMO
Absorption is an effective way to control volatile organic compound (VOC) industrial air pollution, and the key variable in this process is the selection of suitable liquid absorbents to absorb as many organic pollutants as possible. The objective of this study was to prepare a series of high-efficiency absorbents with different proportions of vegetal oil, mineral oil, and waste engine oil, which can be used for toluene absorption. The absorption efficiency (AE), saturated absorption (SA), and effective absorption time (EAT) of various absorbents were systematically analyzed. The results showed that when the inlet concentration of toluene was 8000 mg/m3 and the inlet flow was 1 L/min, the SA capability of vegetal oil, mineral oil, and waste engine oil was 7.15, 12.43, and 18.16 mg/g, respectively. With the 4000 mg/m3 inlet concentration, the SA of the absorber which was made in the ratio of 2:3:1 was increased to 50.93 mg/g. According to the thermodynamic equilibrium and absorption results, it is proved that the influence of the composition of the absorbent on absorption is greater than viscosity. It is also to be noted that the AE of the composite absorbent can still reach more than 80% after three times of heating and air purification.
Assuntos
Poluição do Ar , Compostos Orgânicos Voláteis , Termodinâmica , ToluenoRESUMO
Textile wastewater has been recognized as one of the most difficult to treat environmental problems. Aiming to acquire an excellent treatment effect that could meet the stringent discharge regulations, a series of Cu- and Fe-doped Al-MCM-41 heterogeneous Fenton catalysts with different metal contents (1.21-3.45 wt%) were successfully synthesized by co-precipitation method to degrade Rhodamine B. Their physicochemical properties were analysed by X-ray diffraction, X-ray photoelectron spectroscopy, Fourier transform infrared spectroscopy, nitrogen physisorption and scanning electron microscopy. The incorporation of metal did not alter MCM-41's mesostructure, but increasing the contents of metal would decrease the order of MCM-41s' structure. The effects of temperature, pH, H2O2 dosage, dye concentration and the dosage of catalysts on Rhodamine B degradation were also investigated. It was found that M2 with 2.71 wt% of active metals performed best on Rhodamine B degradation. For the high concentration of Rhodamine B (400 mg/L), the decolorization efficiency could reach 96.0% using only 40 mM H2O2 within 50 min at 60 °C. Further adding 40 mM of H2O2, the chemical oxygen demand removal reached 75.1% after 100 min. M2 showed excellent stability and could be reused at least three times without any obvious deterioration in catalytic activity. M2 fitted well with the Freundlich isotherms and the first-order rate model.
Assuntos
Peróxido de Hidrogênio , Dióxido de Silício , Catálise , RodaminasRESUMO
Vehicular emissions have become a primary anthropogenic source of urban atmospheric volatile halogenated hydrocarbons (VHCs) with the rapid increase of vehicle population, while characteristics of the VHC emissions from different vehicles were rarely systematically investigated. In this study, the on-road tailpipe emissions were sampled from seven in-use vehicles, including two light-duty gasoline vehicles (LDGV), three light-duty diesel trucks (LDDT), one heavy-duty diesel truck (HDDT), and a liquefied petroleum gas-electric hybrid bus (LPGB), using a portable emission measurement system (PEMS) combined with summa canisters, and 35 individual VHC species were identified by a gas chromatography mass spectrometry detector (GC-MSD). Results showed that VHC emissions under urban driving conditions were much higher than those on the suburban roads and highways. The VHC emission factors of LDGV were 1.2 ± 0.34 mg/km and 3.6 ± 1.5, 6.8 ± 0.89, and 1.6 ± 0.28 mg/km for LDDT, HDDT, and LPGB, respectively. For the LDGV, chlorobenzene, 1,2-dichloroethane, and hexachlorobutadiene were the top three VHC species. 1,2-Dichloroethane, trichloromethane, and methyl chloride were the main VHC constituents in the LDDT. Chlorobenzene was the most abundant VOC species for the HDDT, followed by 1,2-dichloroethane and 1,4-dichlorobenzene. The major species for LPGB were 1,2,4-trichlorobenzene, carbon tetrachloride, and benzyl chloride. The major tailpipe VHC species obtained in this study were partial consistent with previous studies with different test methods. The results provide an initial evaluation of the tailpipe VHC emissions, which may provide experimental data support for the refined source apportionment of atmospheric VHCs and the control of vehicular VHCs.
Assuntos
Poluentes Atmosféricos , Hidrocarbonetos Halogenados , Poluentes Atmosféricos/análise , Monitoramento Ambiental , Gasolina/análise , Veículos Automotores , Emissões de Veículos/análiseRESUMO
Polyethylene glycol-600 (PEG-600), as a carrier for slow release of organic substances, can improve the biocompatibility of packing fillers and the construction of biofilms. The gradient experiments were established to evaluate the feasibility of adding different content of PEG-600 to the biofilter for enhancing toluene removal. In particular, the evolution trend of microbial community embedded in packing fillers was measured by 16S rRNA-based gene sequencing. Results showed that the toluene removal efficiency of biofilter with 7.5% adding content of the PEG-600 was greatly improved, and the maximum elimination capacity of 152 g/(m3·h) was obtained. The introduction of PEG-600 enhanced the tolerance ability to withstand the transient impact loading and intensified the production of extracellular polymeric substances and bonding strength of biofilms. It should be noted that the abundance of Pseudomonas and Steroidobacter at genus level increased significantly. The microbial community evolved into a co-degradation system of toluene and PEG-600.
Assuntos
Poluentes Atmosféricos , Microbiota , Biodegradação Ambiental , Filtração , Nutrientes , Polietilenoglicóis , RNA Ribossômico 16S/genética , ToluenoRESUMO
In this study, nitritation-denitrification SBR was successfully begun within 5 days by maintaining a proper condition (pH > 8, DO 0.1-0.5â mg/L and 29.5 ± 0.5°C) and transient excessive aeration would not cause N-NO3 - accumulation. In the start-up stage, FA had an upward trend and reached to 10.98â mg NH3/L, which could entirely inhibit NOB. On the basis of experimental evidence, DO and ORP showed regular trends of variation and the first derivatives of DO and ORP as process control parameters in a nitritation-denitrification SBR was proposed. During the real-time control period, N-NH4 + in the outlet was less than 2â mg N-NH4 +/L and N-NH4 + removal efficiency was 97%, with nitrite accumulation rate (NAR) reaching 98%. After algorithm optimization by using 'Slope', the first derivatives of DO and ORP curves became smooth and interference signals were eliminated. Pre-aeration could promote nitritation rate from 23.76â mg/L/h to 26.27â mg/L/h and increase the transformation rate of N-NH4 + to N-NO2 - from 48.% to 79.6%. The 16S rDNA analysis showed that real-time control could cause a significant difference in microbial community. Nitrosomonas was the dominant strain in AOB and its relative abundance increased from 0.13% to 0.786%. Nitrospira was inhibited and washed out in nitritation-denitrification sludge.
Assuntos
Reatores Biológicos , Desnitrificação , Nitritos , Nitrogênio , EsgotosRESUMO
Corncob-based activated carbon has very good adsorption performance and can provide a favourable growing environment for microorganisms. In this study, a biofilter packed with corncob-based activated carbon was constructed to remove grease and total volatile organic compounds (TVOCs) in kitchen exhaust fume. Results show that the biofilter was suitable for the biodegradation of grease and VOCs, and the maximum elimination capacities (ECmax) were 112 and 235 g/(m3·h) at an empty bed residence time of 3.24 s, respectively. When the pH of the filler dropped to 5.0 ± 0.2, the removal efficiencies (RE) of grease and TVOCs in the biofilter decreased to the minimum values (75% and 77%, respectively). The REmax were respectively 88 ± 4% (for TVOC) at 70% filler moisture content and 90 ± 3% (for grease) at 76% filler moisture content. Molecular characterization results showed Thermobacillus sp. as dominating microbial group in the packing media.