Integrated machine learning algorithms identify KIF15 as a potential prognostic biomarker and correlated with stemness in triple-negative breast cancer.

Cancer stem cells (CSCs) have the potential to self-renew and induce cancer, which may contribute to a poor prognosis by enabling metastasis, recurrence, and therapy resistance. Hence, this study was performed to identify the association between CSC-related genes and triple-negative breast cancer (TNBC) development. Stemness gene sets were downloaded from StemChecker. Based on the online databases, a consensus clustering algorithm was conducted for unsupervised classification of TNBC samples. The variations between subtypes were assessed with regard to prognosis, tumor immune microenvironment (TIME), and chemotherapeutic sensitivity. The stemness-related gene signature was established and random survival forest analysis was employed to identify the core gene for validation experiments and tumor sphere formation assays. 499 patients with TNBC were classified into three subgroups and the Cluster 1 had a better OS than others. After that, WGCNA study was performed to identify genes important for Cluster 1 subtype. Out of all 8 modules, the subtype of Cluster 1 and the yellow module with 103 genes demonstrated the largest positive association. After that, a four-gene stemness-related signature was established. Based on the yellow module, the 39 potential pivotal genes were subjected to the random forest survival analysis to find out the gene that was relatively important for OS. KIF15 was confirmed as the targeted gene by LASSO and random survival forest analyses. In vitro experiments, the downregulation of KIF15 promoted the stemness of TNBC cells. The expression levels of stem cell markers Nanog, SOX2, and OCT4 were found to be elevated in TNBC cell lines after KIF15 inhibition. A stemness-associated risk model was constructed to forecast the clinical outcomes of TNBC patients. The downregulation of KIF15 expression in a subpopulation of TNBC stem cells may promote stemness and possibly TNBC progression.

The role of extracellular organic matter on the cyanobacteria ultrafiltration process.

The membrane fouling caused by extracellular organic matter (EOM) and algal cells and organic matter removal of two typical cyanobacteria (M. aeruginosa and Pseudoanabaena sp.) during ultrafiltration (UF) process were studied in this work. The results showed that EOM had a broad molecular weight (Mw) distribution and the irreversible membrane fouling was basically caused by EOM. Moreover, humic acid and microbial metabolites were major components of EOM of two typical cyanobacteria. Since EOM could fill the voids of cake layers formed by the algal cells, EOM and algal cells played synergistic roles in membrane fouling. Fourier transform infrared spectroscopy analysis indicated that the CH2 and CH3 chemical bonds may play an important role in membrane fouling caused by EOM. Interestingly, the cake layer formed by the algal cells could trap the organic matter produced by algae and alleviate some irreversible membrane fouling. The results also showed that although the cake layer formed by the algal cells cause severe permeate flux decline, it could play a double interception role with UF membrane and increase organic matter removal efficiency. Therefore, when using UF to treat algae-laden water, the balance of membrane fouling and organic matter removal should be considered to meet the needs of practical applications.

Disinfection by-product formation and toxicity evaluation for chlorination with powered activated carbon.

With the deterioration of source water quality, pre-chlorination and pre-addition of powdered activated carbon (PAC) have been widely applied to improve water treatment efficiency, which would lead to PAC exposure to chlorine. Although previous studies reported that some emerging carbon materials (e.g., graphene) could potentially act as disinfection by-product (DBP) precursors, there were few studies paying attention to the interaction between chlorine and the most commonly used carbon material-PAC on the DBP formation. In this study, the DBPs formed by chlorination with and without PAC were investigated, and the DBP toxicities in different systems were evaluated. The results showed that the PAC could react with chlorine and form trihalomethanes (THMs) and haloacetic acids (HAAs). The amount of surface oxygen groups of the PAC increased during the chlorination, with these oxygen groups, especially the meta-positioned -OH groups, facilitating the formation of THMs and HAAs. In the presence of NOM, lower concentrations of THMs and HAAs were observed in the systems with PAC than in those without PAC, demonstrating the critical role of PAC adsorption towards DBP control. The cytotoxicity evaluation indicated that more toxic reaction products between PAC and chlorine were formed besides conventional DBPs. Moreover, the PAC with higher BET surface area and more lactonic function groups formed less toxic DBPs during chlorination, which might reduce health risk for treatment processes with pre-chlorination.

Powdered activated carbon-catalyzed chlorine oxidation of bisphenol-A and methylene blue: Identification of the free radical and effect of the carbon surface functional group.

The effect of powdered activated carbon (PAC) on chlorine oxidation is not well understood, therefore this study was designed to further investigate the chlorine oxidation mechanism with the presence of PAC. The oxidation processes of two model organic pollutants (bisphenol-A and methylene blue) with chlorine were compared in the absence and presence of PAC. The results showed a significant increase in reaction rates with the addition of PAC. Electron spin resonance indicated that the PAC catalyzed the oxidation of chlorine to generate more Cl and O2-. Additionally, the analysis of the surface characteristics of thermally modified PACs under N2 and their corresponding reaction rates revealed that there existed a significant correlation between the CO groups and the catalytic effect. PAC exhibited a much lower reaction rate under H2 modification, which indicated that the π electrons of the basal plane might be involved in the catalysis. Density functional theory calculations confirmed that the various oxygen groups on PAC reduced the activation barrier for HOCl dissociation, particularly the carboxyl group. This investigation provides a better understanding of the interactions between chlorine and activated carbon materials, which could be useful for selecting suitable water treatment agents.

The role of interactions between extracellular organic matter and humic substances on coagulation-ultrafiltration process.

Removals of extracellular organic matter (EOM) derived from cyanobacterium M. aeruginosa and humic acid (HA) in single-component and bi-component systems and the interactions during the coagulation-ultrafiltration (C-UF) process were investigated in this study. In a single-component system, only 23% EOM could be removed by alum at dose as high as 6 mg/L, which induced serious membrane fouling in the following UF process. Interestingly, higher EOM removal efficiency was achieved (increase by about 20%) with the existence of HA and EOM-HA achieved less decline of permeate flux compared with individual EOM C-UF process. Zeta potential and Fourier transform infrared spectroscopy analysis indicated that the interactions of HA and EOM can strengthen charge neutralization and reduce CH2 chemical bonds, which had a positive effect on the coagulation process. In addition, EOM-HA floc had a more open and looser structure than EOM floc, which was more favorable in the UF process. The extended Derjaguin-Landau-Verwey-Overbeek theory indicated that the acid-base interaction energy was mainly reduced, thereby alleviating membrane fouling. The study showed this beneficial interaction between the HA and EOM would enhance the EOM removal efficacy by coagulation and release the membrane fouling caused by EOM.

Characterization and application of poly-ferric-titanium-silicate-sulfate in disperse and reactive dye wastewaters treatment.

A novel coagulant poly-ferric-titanium-silicate-sulfate (PFTS) was synthesized and employed to treat two typical kinds of dye wastewaters-disperse blue and reactive yellow. The results indicated that PFTS with a Si/Fe molar ratio of 0.02 exhibited superior coagulation performance, especially under alkaline condition. The residual turbidity after coagulation by PFTS was only half of that after coagulation by poly-ferric-titanium sulfate (T-PSF). The sludge volume index was also reduced by PFTS compared to T-PSF in reactive dye treatment. Through the structure and morphology investigation of PFTS, it was found that new bonds of Si-O-Fe, Si-O-Ti and Fe-OH (Si-OH) were formed, and multi-branched structures and expanded surface area were generated. Additionally, compared with T-PSF, the floc strength and the floc size were also enhanced by PFTS, which was attributed to the polymerization between polysilicic acid and Fe/Ti which formed multi-branched structures, and finally adsorption and bridging ability of the coagulant was improved. Furthermore, the floc formed in reactive yellow wastewater treatment was larger and looser than that formed in disperse blue wastewater, with poorer strength and recovery ability, which can also interpret the better coagulation efficiency in disperse dye water treatment. From the results of coagulant characterization, zeta potential and flocs properties, it can be inferred that charge neutralization by the positive charged hydrolysate of coagulant was identified as the critical effect in disperse dyes removal, while the sweep and adsorption of metal hydroxyl compound formed during the hydrolysis of coagulants were considered to play a key role in reactive dye removal.

A comparison study of in-situ coagulation and magnetic ion exchange (MIEX) as pre-treatments for ultrafiltration: Evaluating effectiveness of organic matters removals and fouling mitigation.

This work was designed to compare the effectiveness of in-situ coagulation and MIEX as pre-treatments prior to ultrafiltration (UF) to improve organic matter (OM) removal and mitigate membrane fouling. Three kinds of OMs, i.e. salicylic acid (SA), humic acid (HA) and bovine serum albumin (BSA) were employed. The experimental results show that coagulation-UF led to most effective removal of HA (almost 90%), while the SA was uncoagulated and least removable, with the rejection rate of about 55%. Conversely, MIEX present superior ability for removing SA, contributing to additional efficiency of 71.95-77.21% than UF alone. Proper dosage of Al-based coagulants could alleviate flux loss, especially in the cases of HA and BSA. Increasing coagulant dose resulted in continuous decrement of irreversible resistance (Rir), which dominated the membrane fouling development by the SA water. For HA and BSA waters, alternatively, variations of Rr determined the flux declines. Floc compact degree was the decisive factor for Rr for coagulated SA; while for HA and BSA, Rr was most related to the floc size and foulant-foulant interaction. MIEX was most effective for alleviating flux loss when treating the hydrophilic SA with small molecules and for all the cases, MIEX exerted little influence on the Rr values.

Fabrication of a novel Z-scheme g-C3N4/Bi4O7 heterojunction photocatalyst with enhanced visible light-driven activity toward organic pollutants.

In this work, highly efficient g-C3N4/Bi4O7 heterojunction photocatalysts have been successfully fabricated by a facile method. Compared with the bare photocatalysts, the obtained g-C3N4/Bi4O7 hybrid photocatalysts exhibited efficient degradation activity toward methylene blue (MB), phenol, rhodamine B (RhB), and bisphenol A (BPA) under visible light irradiation. The influences of different g-C3N4 contents on the photocatalytic efficiency of the hybrid photocatalysts have been investigated. The results revealed that the g-C3N4/Bi4O7 with g-C3N4 mass ratio of 30% exhibited the best photocatalytic activity. The activity enhancement should be ascribed to the improved visible light adsorption as well as the effective Z-scheme charge transfer according to the energy band theory. The UV-vis diffuse reflectance spectra (DRS) shows that the absorption edge of g-C3N4 move towards longer wavelength with the increment of Bi4O7 component. The strong connection between g-C3N4 and Bi4O7 was investigated using X-ray powder diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), and X-ray photoelectron spectroscopy (XPS). Subsequently, the effective Z-scheme charge transfer has also been verified by using transient photocurrent measurements and electrochemical impedance spectroscopy. Controlled experiments proved that active species of O2- and h+ were produced in the degradation system, which played the major role in the degradation of MB. A possible Z-scheme degradation mechanism over g-C3N4/Bi4O7 hybrid photocatalysts was proposed.